scholarly journals Estrus Behaviour and Reproductive Traits of Pulawska Gilts Associated with Selected Gene Polymorphisms

2021 ◽  
Vol 21 (1) ◽  
pp. 159-172
Author(s):  
Marek Babicz ◽  
Marcin Pastwa ◽  
Magdalena Szyndler-Nędza ◽  
Anna Kozubska-Sobocińska ◽  
Barbara Danielak-Czech ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Gene Polymorphisms ◽  
Rflp Analysis ◽  
Reproductive Traits ◽  
The Other ◽  
Molecular Analyses ◽  
Native Breed ◽  
Hair Roots ◽  
Pcr Rflp ◽  
Pcr Conditions

Abstract Searching for the associations between the gene polymorphism and the reproductive traits is essential in defining the genetic native breed specificity, which distinguishes them from the other breeds. The aim of our study was to determine the associations between mutations in the PRL, PRLR, PTGS2, FUT1 genes and sexual and periparturient activity in native Pulawska gilts. The analysis included 72 animals which gave birth to the first litter. Evaluation of the productive value of gilts accounted for indicators of sexual and periparturient activity as well as reproductive traits. The biological material for molecular analyses was obtained from the hair roots of the gilts. The genotype was verified by PCR RFLP analysis. The primers and PCR conditions were determined on the basis of available literature data. Statistically significant differences (P≤0.05) were found at the PRL locus: gilts of AA genotypes (Ins/Ins) at the PRL locus were characterised by longest farrowing duration compared to gilts of AB genotype (P≤0.05). The analysis of PRLR gene showed that gilts of TT genotype revealed a tendency for later occurrence of estrus signs (first and second estrus) and for the markedly longest farrowings (P≤0.05). With regard to PTGS2 and FUT1 loci, no significant differences were found in the parameters of sexual and periparturient activity of the gilts. However, gilts of FUT1 GG genotype gave birth to and reared the largest first litters (P≤0.05). The results of the studies expand the knowledge about the genetic structure and productivity of Pulawska gilts.

scholarly journals Characterisation of avian Pasteurella multocida strains with PCR-RFLP analysis of the ompH gene

2013 ◽  
Vol 61 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Boglárka Sellyei ◽  
Éva Ivanics ◽  
Tibor Magyar
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Pasteurella Multocida ◽  
Fragment Length Polymorphism ◽  
Rflp Analysis ◽  
The Other ◽  
Pcr Rflp ◽  
H Gene ◽  
Geographic Regions ◽  
Type Strains ◽  
Restriction Fragment Length

The 16 somatic serotype type strains and 60 field isolates of Pasteurella multocida, representing various avian species and geographic regions in Hungary, were characterised by PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the ompH gene with DraI restriction endonuclease. The type strains yielded eight different (I-VIII) profiles. Strains whose PCR fragment was uncut by DraI (profile IV) could be differentiated with HindIII and PvuII restriction endonucleases. Five of the eight PCR-RFLP profiles (I, III, V, VI and VII) were detected among the field strains. Only a correlation of limited strength was found between the classical somatic serotypes and the PCR-RFLP profiles. However, the results confirmed that molecular methods could confidently distinguish serotype A:1 strains from the other serotypes. Moreover, the specific relationship between somatic serotypes and PCR-RFLP types among isolates from turkey raises the possibility of the existence of host-specific clones within the P. multocida population.

scholarly journals The Incidence of Ovulation and Detection of Genes Associated with Ovulation and Twinning Rates in Livestock

2021 ◽  
Author(s):  
Ozden Cobanoglu
Keyword(s):  
Genetic Structure ◽  
Environmental Factors ◽  
Fine Mapping ◽  
Reproductive Traits ◽  
Genomic Region ◽  
Economic Importance ◽  
The Other ◽  
Causative Mutation ◽  
Livestock Species ◽  
Multiple Ovulation

Cattle is a monotocous species that generally produce only one offspring per conception. However, multiple ovulations are a naturally emerging reproductive phenomenon typically controlled by genetic structure and environmental factors. On the other hand, few genes or causative mutations might explain significant genetic variations between animals for the reproductive traits. Studies report different methods, including QTL analysis, fine mapping, GWAS, and MAS selection, to improve such traits due to their economic importance. The recent fine-mapping study, which narrows the genomic region, indeed, influencing multiple ovulation, gives positive signals that causative mutation controlling high ovulation rate may be identified shortly. In conclusion, identifying the major genes that considerably affect ovulation and twinning rates provides the opportunity to increase reproduction efficiency by improving genetic gain in livestock species.

scholarly journals Abiotrophia elegans Strains Comprise 8% of the Nutritionally Variant Streptococci Isolated from the Human Mouth

1999 ◽  
Vol 37 (8) ◽  
pp. 2553-2556 ◽  
Author(s):  
Setsuko Sato ◽  
Taisei Kanamoto ◽  
Masakazu Inoue
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Rflp Analysis ◽  
The Other ◽  
Rrna Gene ◽  
Gene Sequences ◽  
16S Rrna Gene Sequences ◽  
Biochemical Tests ◽  
Pcr Rflp ◽  
Adh Activity

Ninety-one isolates of nutritionally variant streptococci (NVS) that were previously isolated from the human mouth were regarded as consisting of 7 Streptococcus defectivus isolates, 78Streptococcus adjacens isolates, and 6 Gemella morbillorum isolates. However, recent references to the taxonomic reclassification of NVS, from S. defectivusto Abiotrophia defectiva and from S. adjacensto Abiotrophia adiacens, and the newly introduced speciesAbiotrophia elegans as a third Abiotrophiaspecies, emphasize the need for genetic analyses for identification of NVS. When PCR-restriction fragment length polymorphism (RFLP) and phylogenetic distances were examined based on 16S rRNA gene sequences, the results indicated that 7 of the 91 NVS isolates were closely related to A. elegans. These seven isolates consisted of four isolates previously identified as G. morbillorum and three isolates previously identified as S. adjacens. Two isolates previously identified as G. morbillorum were related to A. adiacens. In biochemical tests, A. elegans and the seven isolates related to it possessed arginine dihydrolase (ADH) activity but the other Abiotrophia species did not. As a result, A. elegans strains comprised 8% of the 91 NVS isolates. Our findings suggest that A. elegans, A. adiacens, and A. defectiva exist in the human mouth in proportions of about 1:11:1 and that A. elegans can be genetically distinguished from the other twoAbiotrophia species by PCR-RFLP analysis of 16S rRNA gene sequences and can be biochemically distinguished by ADH activity.

Association of CYP2C19 and UGT1A4 polymorphisms with voriconazole-induced liver injury

2020 ◽  
Vol 17 (1) ◽  
pp. 15-22 ◽  
Author(s):  
Yan Song ◽  
Miao-xin Jia ◽  
Guang Yang ◽  
Xin-yuan Feng ◽  
Dong-hong Yin ◽  
...  
Keyword(s):  
Liver Injury ◽  
Gene Polymorphisms ◽  
Rflp Analysis ◽  
Control Group ◽  
Case Group ◽  
Drug Induced ◽  
Drug Induced Liver Injury ◽  
Significant Difference ◽  
Pcr Rflp ◽  
Genotype And Allele Frequencies

Aim: This study investigated the association between voriconazole-induced liver injury and gene polymorphisms of CYP2C19 and UGT1A4. Materials & methods: Thirty-eight adult patients who received voriconazole therapy were included in the study. Genotype of CYP2C19 was detected using gene chip hybrid analysis. The UGT1A4 142T>G was genotyped using PCR-RFLP analysis. Results: Ten patients (26.3%) had voriconazole-induced liver injury and were considered as the case group There was no significant difference between the two groups in genotype and allele frequencies of CYP2C19*2 and UGT1A4 142T>G (p > 0.05), however, the GA frequency of CYP2C19 *3 in the drug-induced liver injury case group was higher than that in the control group (p < 0.05). Compared with patients carrying *1/*1 or *1/*2, there was no significant difference in voriconazole trough concentration of the patients with *1/*3 (p > 0.05). Conclusion: There was no significant correlation between voriconazole-induced liver injury and gene polymorphisms of CYP2C19 and UGT1A4.

scholarly journals Genotyping of the prevalent Chlamydia trachomatis strains involved in cervical infections in women in Ahvaz, Iran

2010 ◽  
Vol 59 (9) ◽  
pp. 1023-1028 ◽  
Author(s):  
Behrouz Taheri Beni ◽  
Hossein Motamedi ◽  
Mohammad Roayaei Ardakani
Keyword(s):  
Chlamydia Trachomatis ◽  
Clinical Symptoms ◽  
Age Groups ◽  
Lower Abdominal Pain ◽  
Rflp Analysis ◽  
The Other ◽  
Pcr Rflp ◽  
First Time ◽  
Genotype F ◽  
Genotype E

To determine the prevalence of cervical Chlamydia trachomatis genotypes in Iran for the first time and their association with three clinical symptoms/signs, i.e. abnormal vaginal discharge, lower abdominal pain (LAP) and swab-induced bleeding, and patient age, 620 cervical specimens were obtained from women with symptomatic genital infection referred to gynaecological clinics and 108 C. trachomatis-positive specimens were genotyped by direct omp1 gene PCR-RFLP analysis. Eight genotypes were identified. The most prevalent genotype was E (31.5 %), followed by F (23.1 %), D/Da (13 %), K (9.2 %), I (8.3 %), G (7.5 %), H (5.5 %) and J (1.9 %). For analysing the association of C. trachomatis genotypes with symptoms/signs and age, P-values were separately evaluated for genogroups and genotypes. The analysis of genogroups showed that women infected with genogroup F/G manifested the symptom of LAP significantly more often than those infected with the other genogroups (P=0.02), while the analysis of genotypes revealed that women infected with genotype F reported LAP slightly more often than women infected with the other genotypes (P=0.08). No significant correlation between genogroups and age was found; however, genotype E was somewhat less prevalent among women aged 25–34 years than among other age groups (P=0.08).

Genetic polymorphisms of cytochrome P450 enzymes: CYP2C9, CYP2C19, CYP2D6, CYP3A4, and CYP3A5 in the Croatian population

2017 ◽  
Vol 32 (1) ◽  
Author(s):  
Lana Ganoci ◽  
Tamara Božina ◽  
Nikica Mirošević Skvrce ◽  
Mila Lovrić ◽  
Petar Mas ◽  
...  
Keyword(s):  
Rflp Analysis ◽  
The Other ◽  
Published Data ◽  
Cytochrome P450 Enzymes ◽  
Chain Reaction ◽  
Allelic Variants ◽  
Mediterranean Populations ◽  
Pcr Rflp ◽  
Polymerase Chain ◽  
European Populations

AbstractBackground:Data on the frequency of pharmacogenetic polymorphisms in the Croatian population are limited. We determined and analyzed frequencies for the most importantMethods:2637 subjects were included. Genotyping was performed by real-time polymerase chain reaction (PCR) using TaqMan® DME or TaqMan® SNP Genotyping Assays, and by PCR, and PCR-RFLP analysis.Results:ForConclusions:The frequency of the CYP allelic variants, genotypes, and predicted phenotypes in the Croatian population is in accordance with the other European populations, between the values of published data for Middle European and Mediterranean populations.

Molecular epidemiology ofBartonellaspecies isolated from ground squirrels and other rodents in northern California

2016 ◽  
Vol 144 (9) ◽  
pp. 1837-1844 ◽  
Author(s):  
A. C. ZIEDINS ◽  
B. B. CHOMEL ◽  
R. W. KASTEN ◽  
A. M. KJEMTRUP ◽  
C.-C. CHANG
Keyword(s):  
Rflp Analysis ◽  
Ground Squirrels ◽  
The Other ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Northern California ◽  
Wild Rodents ◽  
Neotoma Fuscipes ◽  
Pcr Rflp ◽  
California Ground Squirrels

SUMMARYBartonellaspp. are endemic in wild rodents in many parts of the world. A study conducted in two northern California counties (Sonoma and Yolo) sampling California ground squirrels (Otospermophilus beecheyi) and four other rodent species (Peromyscus maniculatus, P. boylii, P. trueiandNeotoma fuscipes) led to the isolation of small Gram-negative bacilli which were identified asBartonellaspp. based on colony morphology, polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) and partial gene sequencing. Overall,Bartonellaspp. were isolated from the blood of 71% (32/45) of the ground squirrels and one third (22/66) of the other rodents. PCR–RFLP analysis of thegltA and 16S rRNA genes yielded seven unique profiles, four for the ground squirrels and three for the other rodents. Isolates from each PCR–RFLP profiles were submitted for partial sequencing. Ground squirrel isolates were most closely related toB. washoensis, whereas the other rodent isolates were closest toB. vinsoniisubsp.vinsoniiandB. vinsoniisubsp.arupensis. Two of these three species or subspecies are known zoonotic agents.

scholarly journals Molecular Typing of Methicillin ResistantStaphylococcus aureusClinical Isolates on the Basis of Protein A and Coagulase Gene Polymorphisms

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Nancy Younis Omar ◽  
Hala Abdel Salam Ali ◽  
Reem Abdel Hameed Harfoush ◽  
Engy Hamdy El Khayat
Keyword(s):  
Molecular Typing ◽  
Gene Polymorphisms ◽  
Protein A ◽  
Rflp Analysis ◽  
Restriction Digestion ◽  
Banding Patterns ◽  
Pcr Rflp ◽  
Pcr Products ◽  
Mrsa Strains

Increased frequency of methicillin-resistantStaphylococcus aureus(MRSA) in hospitalized patients requires rapid and reliable characterization of isolates for control of MRSA spread in hospitals. This study evaluated polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) as a molecular typing technique for MRSA strains on the basis of protein A (spa) and coagulase (coa) gene polymorphisms to verify their ability in assessing the relatedness of isolates. Seventy-five MRSA isolates, from different ICUs of Alexandria University Main Hospital, were characterized using antibiotyping and PCR-RFLP analysis ofcoaandspagenes. Thirty-two antibiotypes were identified.coagene PCR generated 3 types and 10 subtypes of band patterns.HaeIIIrestriction digestion of amplifiedcoagene products produced 5 major banding patterns and 12 subtypes.spagene PCR products generated 4 major and 11 minor types, and theirHaeIIrestriction digestion showed 5 major and 12 minor banding patterns. The combinedcoaandspaRFLP patterns generated 22 combined R types. Typing usingcoaPCR and PCR-RFLP had the same discriminatory index (DI) value (0.64), which was comparable to that of bothspaPCR and PCR-RFLP techniques (0.68). The combined grouping increased the DI value to 0.836. The current study revealed that testing for multiple gene polymorphisms is more useful for local epidemiologic purposes.

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