scholarly journals Comparison of Chromogenic Selective Media for the Detection of Cronobacter spp. (Enterobacter sakazakii)

2018 ◽  
Vol 23 (1) ◽  
pp. 27-33 ◽  
Author(s):  
HAJIME TERAMURA ◽  
NORIKO FUKUDA ◽  
YUMIKO OKADA ◽  
HIROKAZU OGIHARA
Keyword(s):  
Enterobacter Sakazakii ◽  
Selective Media

Enterobacteriaceae in Dehydrated Powdered Infant Formula Manufactured in Indonesia and Malaysia

2006 ◽  
Vol 69 (12) ◽  
pp. 3013-3017 ◽  
Author(s):  
SRI ESTUNINGSIH ◽  
CLAUDIA KRESS ◽  
ABDULWAHED A. HASSAN ◽  
ÖMER AKINEDEN ◽  
ELISABETH SCHNEIDER ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Health Risk ◽  
Southeast Asia ◽  
Infant Formula ◽  
Enterobacter Cloacae ◽  
Powdered Infant Formula ◽  
Enterobacter Sakazakii ◽  
Opportunistic Pathogens ◽  
Selective Media

To determine the occurrence of Salmonella and Shigella in infant formula from Southeast Asia, 74 packages of dehydrated powdered infant follow-on formula (recommended age, >4 months) from five different manufacturers, four from Indonesia and one from Malaysia, were analyzed. None of the 25-g test portions yielded Salmonella or Shigella. However, further identification of colonies growing on selective media used for Salmonella and Shigella detection revealed the frequent occurrence of several other Enterobacteriaceae species. A total of 35 samples (47%) were positive for Enterobacteriaceae. Ten samples (13.5%) from two Indonesian manufacturers yielded Enterobacter sakazakii. Other Enterobacteriaceae isolated included Pantoea spp. (n = 12), Escherichia hermanii (n = 10), Enterobacter cloacae (n = 8), Klebsiella pneumoniae subsp. pneumoniae (n = 3), Citrobacter spp. (n = 2), Serratia spp. (n = 2), and Escherichia coli (n = 2). To our knowledge, this is the first report to describe the contamination of dehydrated powdered infant formula from Indonesia with E. sakazakii and several other Enterobacteriaceae that could be opportunistic pathogens. Improper preparation and conservation of these products could result in a health risk for infants in Indonesia.

scholarly journals Performance of Media for Recovering Stressed Cells of Enterobacter sakazakii as Determined Using Spiral Plating and Ecometric Techniques

2005 ◽  
Vol 71 (12) ◽  
pp. 7661-7669 ◽  
Author(s):  
J. B. Gurtler ◽  
L. R. Beuchat
Keyword(s):  
Infant Formula ◽  
Fecal Coliform ◽  
Colony Development ◽  
Powdered Infant Formula ◽  
Enterobacter Sakazakii ◽  
Order Of Growth ◽  
Growth Indices ◽  
Selective Media ◽  
General Order ◽  
Stressed Cells

ABSTRACT A study was done to determine the performance of differential, selective media for supporting resuscitation and colony development by stressed cells of Enterobacter sakazakii. Cells of four strains of E. sakazakii isolated from powdered infant formula were exposed to five stress conditions: heat (55°C for 5 min), freezing (−20°C for 24 h, thawed, frozen again at −20°C for 2 h, thawed), acidic pH (3.54), alkaline pH (11.25), and desiccation in powdered infant formula (water activity, 0.25; 21°C for 31 days). Control and stressed cells were spiral plated on tryptic soy agar supplemented with 0.1% pyruvate (TSAP, a nonselective control medium); Leuschner, Baird, Donald, and Cox (LBDC) agar (a differential, nonselective medium); Oh and Kang agar (OK); fecal coliform agar (FCA); Druggan-Forsythe-Iversen (DFI) medium; violet red bile glucose (VRBG) agar; and Enterobacteriaceae enrichment (EE) agar. With the exception of desiccation-stressed cells, suspensions of stressed cells were also plated on these media and on R&F Enterobacter sakazakii chromogenic plating (RF) medium using the ecometric technique. The order of performance of media for recovering control and heat-, freeze-, acid-, and alkaline-stressed cells by spiral plating was TSAP > LBDC > FCA > OK, VRBG > DFI > EE; the general order for recovering desiccated cells was TSAP, LBDC, FCA, OK > DFI, VRBG, EE. Using the ecometric technique, the general order of growth indices of stressed cells was TSAP, LBDC > FCA > RF, VRBG, OK > DFI, EE. The results indicate that differential, selective media vary greatly in their abilities to support resuscitation and colony formation by stressed cells of E. sakazakii. The orders of performance of media for recovering stressed cells were similar using spiral plating and ecometric techniques, but results from spiral plating should be considered more conclusive.

scholarly journals Evaluation of Selective Media Containing Iron Source and Alpha-Glucosidase Substrates for Enterobacter sakazakii (Cronobacter spp.) Detection

2021 ◽  
Vol 39 (1) ◽  
pp. 9-19
Author(s):  
Jung-Whan Chon ◽  
Kun-Ho Seo ◽  
Jin-Hyeok Yim ◽  
Dongryeoul Bae ◽  
Binn Kim ◽  
...  
Keyword(s):  
Enterobacter Sakazakii ◽  
Selective Media ◽  
Iron Source ◽  
Alpha Glucosidase

Detection of OMPA gene of Enterobacter sakazakii from dried milk sample in Iraq

2020 ◽  
Vol 23 (02) ◽  
pp. 155-165
Author(s):  
Mohammed Mosa Jaafar ◽  
Salwa Khudadad Kalid ◽  
Mustafa Hasan Zaine khetam ◽  
Habeeb Rasooll
Keyword(s):  
Milk Sample ◽  
Enterobacter Sakazakii ◽  
Ompa Gene

Use of gene probes for the detection of quiescent enteric bacteria in marine and fresh waters

1995 ◽  
Vol 31 (5-6) ◽  
pp. 291-298
Author(s):  
Sally A. Anderson ◽  
Gillian D. Lewis ◽  
Michael N. Pearson
Keyword(s):  
Membrane Filtration ◽  
Enteric Bacteria ◽  
Probe Method ◽  
Detection Methods ◽  
23S Rrna ◽  
Specific Gene ◽  
Gene Probe ◽  
Selective Media ◽  
Selective Enrichment ◽  
E Coli

Specific gene probe detection methods that utilise a non-selective culturing step were tested for the ability to recognise the presence of quiescent enteric bacteria (Escherichia coli and Enterococcus faecalis ) within illuminated freshwater and seawater microcosms. An E. coli specific uidA gene probe and a 23S rRNA oligonucleotide probe for Enterococci were compared with recoveries using membrane filtration and incubation on selective media (mTEC and mE respectively). From these microcosm experiments a greater initial detection (from 4 hours to 1 day) of E. coli and Ent. faecalis using gene probe methods was observed. Additionally, a comparison of E. coli direct viable counts (DVC) in sunlight exposed microcosms with recoveries by selective media and gene probe methods revealed a large number of viable non-culturable cells. This suggests that enumeration of E. coli by a gene probe method is limited by the replication of the bacteria during the initial non-selective enrichment step. The detection of stressed Ent. faecalis by the oligonucleotide gene probe method was significantly greater than recovery on selective mE agar, indicating an Enterococci non-growth phase.

Microbiological performance and salmonella dynamics in a wastewater depuration pond system of southeastern spain

1995 ◽  
Vol 31 (12) ◽  
pp. 239-248 ◽  
Author(s):  
Ana Emparanza-Knörr ◽  
Francisco Torrella
Keyword(s):  
Fecal Coliform ◽  
No Reduction ◽  
Agar Medium ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Fecal Coliforms ◽  
Selective Media ◽  
E Coli ◽  
The Village ◽  
Final Effluent

The Salmonella presence and the microbiological quality indicators, total and fecal coliforms and coliphages of E. coli C, have been studied in a overloaded wastewater lagoon system treating urban wastewatrers of the village of Guardamar del Segura (Alicante, Spain). Classical microbiological technology to detect salmonellae was used, including pre-enrichment, enrichment, selective media plating and biochemical and serological confirmation. Water was physicochemically characterized according to COD, SS, temperature, pH and dissolved oxygen. The selective migration step through Rappaport-Vassiliadis semisolid agar medium was essential for the consistent detection of Salmonella in the different lagoon effluents. Total and fecal coliform levels of up to 105-106 MPN/100 ml were detected in the final effluent. High coliphage concentrations of 103-104 pfu/ml were also found in the effluent waters. Salmonella was always detected in 100 ml samples and eventually reached an order of value of 103 MPN/100 ml. Total coliform reduction was higher in the anaerobic ponds whereas fecal coliforms were more efficiently eliminated in the facultative (mostly “anoxic”) lagoons. Coliphage reduction was higher in the facultative lagoons when compared to the anaerobic ponds. On many occasions, no reduction or eventual increment of the concentration of salmonellae was detected in the effluents from the anaerobic ponds compared to concentrations of the patohogen in the influent raw wasterwaters. The possibility exists for a capacity of Salmonella to multiply in the anoxic phase of the wastewater treatment, but the presence of microorganisms in raw sewage waters which could maskSalmonella detection with the enrichment methodology employed cannot be ruled out.

Biotipe Isolat Lokal Enterobacter sakazakii

2016 ◽  
Vol 10 (1) ◽  
pp. 29-38
Author(s):  
Iza Saufani
Keyword(s):  
Enterobacter Sakazakii

Microbiological Examination of Vegetable Seed Sprouts in Korea

2009 ◽  
Vol 72 (4) ◽  
pp. 856-859 ◽  
Author(s):  
HOIKYUNG KIM ◽  
YOUNGJUN LEE ◽  
LARRY R. BEUCHAT ◽  
BONG-JUNE YOON ◽  
JEE-HOON RYU
Keyword(s):  
Microbiological Quality ◽  
Aerobic Bacteria ◽  
Escherichia Coli O157 ◽  
Enterobacter Sakazakii ◽  
Department Stores ◽  
E Coli ◽  
Traditional Markets ◽  
Radish Sprouts ◽  
Online Stores

Sprouted vegetable seeds used as food have been implicated as sources of outbreaks of Salmonella and Escherichia coli O157:H7 infections. We profiled the microbiological quality of sprouts and seeds sold at retail shops in Seoul, Korea. Ninety samples of radish sprouts and mixed sprouts purchased at department stores, supermarkets, and traditional markets and 96 samples of radish, alfalfa, and turnip seeds purchased from online stores were analyzed to determine the number of total aerobic bacteria (TAB) and molds or yeasts (MY) and the incidence of Salmonella, E. coli O157:H7, and Enterobacter sakazakii. Significantly higher numbers of TAB (7.52 log CFU/g) and MY (7.36 log CFU/g) were present on mixed sprouts than on radish sprouts (6.97 and 6.50 CFU/g, respectively). Populations of TAB and MY on the sprouts were not significantly affected by location of purchase. Radish seeds contained TAB and MY populations of 4.08 and 2.42 log CFU/g, respectively, whereas populations of TAB were only 2.54 to 2.84 log CFU/g and populations of MY were 0.82 to 1.69 log CFU/g on alfalfa and turnip seeds, respectively. Salmonella and E. coli O157:H7 were not detected on any of the sprout and seed samples tested. E. sakazakii was not found on seeds, but 13.3% of the mixed sprout samples contained this potentially pathogenic bacterium.

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