scholarly journals Isolation and characterization of a species-specific DNA fragment for detection of Candida albicans by polymerase chain reaction.

1992 ◽  
Vol 30 (4) ◽  
pp. 894-900 ◽  
Author(s):  
Y Miyakawa ◽  
T Mabuchi ◽  
K Kagaya ◽  
Y Fukazawa
Keyword(s):  
Polymerase Chain Reaction ◽  
Candida Albicans ◽  
Chain Reaction ◽  
Isolation And Characterization ◽  
Dna Fragment ◽  
Polymerase Chain ◽  
Species Specific

scholarly journals Isolation and Characterization of Micro-organisms with Industrial Importance From Sisal Bole Rots

2013 ◽  
Vol 34 (1) ◽  
pp. 26-35
Author(s):  
Consolatha J.N. Mhaiki ◽  
Enock Masanja ◽  
Jamidu H.Y. Katima ◽  
Gunaraths Rajarao ◽  
Gunnel Dalhammar
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Isolation And Characterization ◽  
Brevundimonas Diminuta ◽  
Industrial Use ◽  
Brevundimonas Vesicularis ◽  
Polymerase Chain ◽  
The Individual ◽  
Micro Organisms

Investigation of microorganisms naturally acclimatized to Agave hybrid H 11648 (sisal bole rot) was conducted, with the aim of isolating and characterizing Aspergillus niger strains for industrial use. Microorganism were identified morphologically and then confirmation made by polymerase chain reaction (PCR). Results showed the existence of four major groups, listed in order of abundances as follows; Aspergilli (36.0±0.8) %, Penicillin (28.0±0.1) %, Yeast (15.0±1.6) %and Fusarium (10.0±0.12) %. The main groups of Aspergilli strains were A. nidulans, A. tamari and A. niger in ratios (3:2:2), respectively. Several endo-spore forming non-enteric gram (-) rods and coccid bacteria identified by API20 NE identification systemincluded,Brevundimonas diminuta sp, Shewanella putrefaciens sp, Brevundimonas vesicularis sp and Pasteurella sp. Results showed that sisal bole rot stems hosts a high bio-diversity of microorganism species other than A. niger. Exploitation of the individual strains is recommended. This could eventually produce strains forprecursors of industrially and therapeutically metabolites.

Routine identification of four sympatric species of calanoid copepods Pseudocalanus spp. in the Atlantic Arctic using a species-specific polymerase chain reaction

2020 ◽  
Vol 48 (1) ◽  
pp. 62-72
Author(s):  
E. A. Ershova
Keyword(s):  
Polymerase Chain Reaction ◽  
Life Cycles ◽  
The Arctic ◽  
Relative Distribution ◽  
Chain Reaction ◽  
Specific Polymerase Chain Reaction ◽  
Routine Identification ◽  
Polymerase Chain ◽  
Species Specific ◽  
Plankton Communities

Сalanoid copepods of the genus Pseudocalanus play an important role in the plankton communities of the Arctic and boreal seas, often dominating in numbers and constituting a significant proportion of the biomass of zooplankton. Despite their high presence and significance in the shelf plankton communities, species-specific studies of the biology of these are significantly hampered by extremely small morphological differences between them, especially at the juvenile stages, at which they are virtually indistinguishable. In this paper, we describe a new, routine and low-cost molecular method for identifying all Pseudocalanus species found in the Atlantic sector of the Arctic: the Arctic P. acuspes, P. minutus and the boreal P. moultoni and P. elongatus, and apply it to describe the relative distribution of these species in four locations of the Arctic and sub-Arctic. With this method, species-specific polymerase chain reaction (ssPCR), mass identification of individuals of any developmental stage, including nauplii, is possible. This method can serve as an excellent tool for studying the species-specific biology of this group, describing their life cycles, as well as monitoring changes in Arctic marine ecosystems under the influence of changing climate.

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