Brevundimonas vesicularis Peritonitis in a Chronic Peritoneal Dialysis Patient

Gram-negative peritonitis in chronic peritoneal dialysis patients is difficult to treat and may result in catheter loss. <i>Brevundimonas vesicularis</i> is a Gram-negative rod bacterium which rarely causes infections in humans. A 41-year-old male receiving continuous cycling peritoneal dialysis for 5 months developed culture-negative peritonitis. He failed initial empiric treatment with intraperitoneal vancomycin and levofloxacin and thereafter intravenous gentamicin. <i>B. vesicularis</i> resistant to levofloxacin was isolated from the peritoneal fluid 21 days after his initial symptoms. Despite treatment with intravenous ceftriaxone and oral amoxicillin-clavulanate, the infection persisted, which required removal of the peritoneal catheter in order to cure this infection. We describe the features of <i>B. vesicularis</i> infection in our patient and the rarely reported additional cases.

Plant debris are hotbeds for pathogenic bacteria on recreational sandy beaches

On recreational sandy beaches, there are guidelines for the management of bacterial pollution in coastal waters regarding untreated sewage, urban wastewater, and industrial wastewater. However, terrestrial plant debris on coastal beaches can be abundant especially after floods and whilst it has rarely been considered a concern, the bacterial population associated with this type of pollution from the viewpoint of public health has not been adequately assessed. In this study, microbes associated with plant debris drifting onto Kizaki Beach in Japan were monitored for 8 months throughout the rainy season, summer, typhoon season, and winter. Here we show that faecal-indicator bacteria in the plant debris and sand under the debris were significantly higher than the number of faecal bacteria in the sand after a 2015 typhoon. When we focused on specific pathogenic bacteria, Brevundimonas vesicularis and Pseudomonas alcaligenes were commonly detected only in the plant debris and sand under the debris during the survey period. The prompt removal of plant debris would therefore help create safer beaches.

Brevundimonas Vesicularis: Unusual Cause of Bacteraemia in Otherwise Healthy 8 Months old Premature Child: Case Report

Brevundimonas vesicularis, a non-fermenting gram-negative bacterium, has rarely been diagnosed as a cause of infection in an otherwise healthy child. In this report,we describe Brevundimonas vesicularis bacteremia, in an 8months old healthy girl who was treated successfully with intravenous piperacillin-tazobactam.

Contrasting Patterns of Microbial Communities in Glacier Cryoconite of Nepali Himalaya and Greenland, Arctic

To understand the microbial composition and diversity patterns, cryoconite granules were collected from two geographical areas, i.e., Nepali Himalaya and Greenland, Arctic. 16S rRNA, ITS and the D1/D2 domain sequencing techniques were used for characterization of microbial communities of the four glaciers. The total 13 species of bacteria such as Bacillus aryabhattai, Bacillus simplex, Brevundimonas vesicularis, Cryobacterium luteum, Cryobacterium psychrotolerans, Dermacoccus nishinomiyaensis, Glaciihabitans tibetensis, Leifsonia kafniensis, Paracoccus limosus, Polaromonas glacialis, Sporosarcina globispora, Staphylococcus saprophyticus, Variovorax ginsengisoli, and 4 species of fungi such as Goffeauzyma gilvescens, Mrakia robertii, Dothideomycetes sp., Helotiales sp. were recorded from Nepali Himalaya. Among these, 12 species of bacteria and 4 species of fungi are new contributions to Himalaya. In contrast to this, six species of bacteria such as Bacillus cereus, Cryobacterium psychrotolerans, Dermacoccus nishinomiyaensis, Enhydrobacter aerosaccus, Glaciihabitans tibetensis, Subtercola frigoramans, and nine species of fungi such as Goffeauzyma gilvescens, Mrakia robertii, Naganishia vaughanmartiniae, Piskurozyma fildesensis, Rhodotorula svalbardensis, Alatospora acuminata, Articulospora sp., Phialophora sp., Thelebolus microspores, and Dothideomycetes sp.), were recorded from Qaanaaq, Isunnguata Sermia and Thule glaciers, Greenland. Among these, five species of bacteria and seven species of fungi are new contributions to Greenland cryoconite. Microbial analyses indicate that the Nepali Himalayan cryoconite colonize higher numbers of microbial species compared to the Greenland cryoconite.

Mikroorganisme sebagai agen bioremediasi limbah merkuri (Hg) penambangan emas

Banyaknya kegiatan Penambangan Emas Tanpa Ijin (PETI) di Indonesia menjadi sumber utama terjadinya pencemaran limbah merkuri. Merkuri merupakan salah satu jenis polutan logam berat yang bersifat toksik. Merkuri menimbulkan masalah serius pada kesehatan manusia karena dapat terakumulasi pada tubuh dan bersifat neurotoksin. Beberapa bakteri memiliki kemampuan resisten terhadap merkuri. Sejumlah bakteri yang resisten terhadap limbah merkuri telah diisolasi dari berbagai lokasi pertambangan emas di Indonesia. Beberapa bakteri lokal tersebut dirangkum dalam penelitian ini. Bakteri tersebut diantaranya yaitu : Bacillus sp., Escherichia coli, Enterobacter cloacea, Enterobacter aerogenes, Klebsiella pneumoniae, Alcaligenes sp., Pseudomonas sp., Enterobacter agglomerans, Proteus vugaris, Proteus mirabilis, Enterobacter hafniae, Kliebsella sp., Lactobacillus sp., Morganella morganii, Brevundimonas vesicularis, Nitrococcus mobilis, Fusobacterium aquatile, dan Fusobacterium necrogenes. Bakteri yang resisten terhadap merkuri tersebut dapat dimanfaatkan sebagai agen untuk remediasi wilayah yang tercemar limbah merkuri

Scombroid Poisoning with Concurrent Brevundimonas Septicemia: A Unique Case Report and Brief Literature Review

Scombroid poisoning is a predominantly self-limited illness associated with ingestion of poorly handled fish. It is not frequently associated with bacteremia and has never been described with Brevundimonas septicemia. We describe a case of a man who presented in shock with histamine poisoning after ingesting sushi. Blood cultures grew an uncommon pathogen, Brevundimonas vesicularis. This case demonstrates systemic bacterial infection in the setting of histamine poisoning, which is an atypical presentation for a well-known foodborne illness.

A Systematic Detection for Brucellosis at Chronic Stage of Infection in Semen of Sheep and Saanen Goats

The study was conducted in a herd (n: 244) in which goats (n: 206) and sheep (n:38) had a history of brucellosis in Bursa which is located in Northwestern of Turkey between the years 2012-2014. For the detection of Brucella spp. and the other zoonotic bacterial agents, semen samples were taken from Saanen goats (n: 35) and rams (n: 8). Samples were tested by routine diagnostic procedures and PCR. The serum samples of male animals were also tested for Brucellosis by C-ELISA and I-ELISA. The culture results represented Trueperella pyogenes (n:2), Pasteurella pneumotopica (n: 5), Esherichia coli (n: 3), Aeromonas salmonicida subs. Salmonicida (1), Brevundimonas vesicularis (n: 2) and Mycoplasma bovigenitalium (n: 1) and Mycoplasma arginini (n: 1) from semen samples. Rams had no symptoms due to epididymitis or epididymoorchitis in clinical examination, but two bucks showed orchitis and they were serologically positive for brucellosis. Also, one seronegative buck showed epididymitis in a flock. There were no statistically significant differences between the serologically positive and negative animals in an examination of semen samples in terms of their volume, concentration, mass activity, motility and defectivity rate for acrosome. Although 20 of the serum samples were negative for anti-Brucella antibody, 23 of them were serologically positive for brucellosis. As a result of this study, Brucellae were not detected by bacteriologically and molecularly while there were some positive serum samples for brucellosis. This could be attributed that these samples might have been collected from chronically infected animals in which animals generally do not shed the organisms. Therefore, it was thought that sampling with regular intervals might help for the definitive incidence of brucellosis.

Genetic potential of bacteria isolated from cadmium contaminated soils

Aim. Determination of taxonomic position and resistance to Cd2+, Cu2+, Ni2+, Co2+, CrO42- of isolated bacteria from cadmium-contaminated soils. Methods. Bacterial cultures were isolated by ten-fold dilutions and further cultivation on agarized nutrient medium. Taxonomic position of isolated cultures was determined by molecular-genetic methods using GenBank and BLAST. Bacterial resistance to toxic metals was determined by strains cultivation in liquid medium in the presence of concentration gradient of Cd2+, Cu2+, Ni2+, Co2+, CrO42-. Results. Isolated cultures were highly resistance to cadmium. These bacterial cultures were identified as Brevundimonas vesicularis and Cupriavidus gilardii by phenotypic and molecular-genetic characteristics. Their resistance was in one order higher comparing to generally accepted. Conclusions. Isolated strains are promising for using in novel industrial biotechnologies for metal containing wastewater treatment. Keywords: metalresistance, toxic metals, taxonomic position, Cupriavidus gilardii, Brevundimonas vesicularis.