scholarly journals Performance of MALDI-TOF Mass Spectrometry to Determine the Sex of Mosquitoes and Identify Specific Colonies from French Polynesia

2021 ◽  
Author(s):  
Fatou Kiné Fall ◽  
Maureen Laroche ◽  
Hervé Bossin ◽  
Didier Musso ◽  
Philippe Parola
Keyword(s):  
Mass Spectrometry ◽  
Geographic Origin ◽  
French Polynesia ◽  
Correct Identification ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Aedes Polynesiensis ◽  
Tof Ms

Mosquitoes are the main arthropod vectors of infectious diseases in humans. The current methods for mosquito identification include morphological and molecular methods. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), now routinely used for bacterial identification, has recently emerged in the field of entomology. The aim of this study was to use MALDI-TOF MS to identify mosquito colonies from French Polynesia. Five hundred specimens from French Polynesia belonging to three species, Aedes aegypti, Aedes polynesiensis, and Culex quinquefasciatus, were included in the study. Testing the legs of these mosquitoes by MALDI-TOF MS revealed a 100% correct identification of all specimens at the species level. The MALDI-TOF MS profiles obtained allowed differentiation of male from female mosquitoes and the specific identification of female mosquito colonies of the same species but different geographic origin.

scholarly journals Application of MALDI-TOF mass spectrometry in clinical diagnostic microbiology

2014 ◽  
Vol 8 (09) ◽  
pp. 1081-1088 ◽  
Author(s):  
Elena De Carolis ◽  
Antonietta Vella ◽  
Luisa Vaccaro ◽  
Riccardo Torelli ◽  
Teresa Spanu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Drug Susceptibility ◽  
Biochemical Tests ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Bacteria And Fungi ◽  
Diagnostic Microbiology ◽  
Tof Ms

Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) has recently emerged as a powerful technique for identification of microorganisms, changing the workflow of well-established laboratories so that its impact on microbiological diagnostics has been unparalleled. In comparison with conventional identification methods that rely on biochemical tests and require long incubation procedures, MALDI-TOF MS has the advantage of identifying bacteria and fungi directly from colonies grown on culture plates in a few minutes and with simple procedures. Numerous studies on different systems available demonstrate the reliability and accuracy of the method, and new frontiers have been explored besides microbial species level identification, such as direct identification of pathogens from positive blood cultures, subtyping, and drug susceptibility detection.

scholarly journals Current Status of Matrix-Assisted Laser Desorption/Ionization–Time-of-Flight Mass Spectrometry (MALDI-TOF MS) in Clinical Diagnostic Microbiology

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4775
Author(s):  
Sachio Tsuchida ◽  
Hiroshi Umemura ◽  
Tomohiro Nakayama
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Clinical Applications ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Tof Ms ◽  
Matrix Assisted Laser

Mass spectrometry (MS), a core technology for proteomics and metabolomics, is currently being developed for clinical applications. The identification of microorganisms in clinical samples using matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry (MALDI-TOF MS) is a representative MS-based proteomics application that is relevant to daily clinical practice. This technology has the advantages of convenience, speed, and accuracy when compared with conventional biochemical methods. MALDI-TOF MS can shorten the time used for microbial identification by about 1 day in routine workflows. Sample preparation from microbial colonies has been improved, increasing the accuracy and speed of identification. MALDI-TOF MS is also used for testing blood, cerebrospinal fluid, and urine, because it can directly identify the microorganisms in these liquid samples without prior culture or subculture. Thus, MALDI-TOF MS has the potential to improve patient prognosis and decrease the length of hospitalization and is therefore currently considered an essential tool in clinical microbiology. Furthermore, MALDI-TOF MS is currently being combined with other technologies, such as flow cytometry, to expand the scope of clinical applications.

scholarly journals Iodine-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Sai Raghuveer Chava
Keyword(s):  
Mass Spectrometry ◽  
Time Of Flight ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Desorption Ionization ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Tof Ms

Adsorption and desorption of iodine-containing α-cyano-4-hydroxycinnamic acid (aCCa) matrix species were studied using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The MALDI-TOF MS method showed that ca. 0.8- 1.4 monolayer (~ 100 ppm) of iodine-containing species was adsorbed at the surface and assisted in desorption and ionization of a protein digest peptides or peptides varying in isoelectric potential. At low laser power, desorption of analytes in protonated and sodiated form was observed but not iodine cluster relative to aCCa without iodine, suggesting a two-electron reduction process to form the protonated pseudo molecular ion, although adsorption on the surface would lead to oxidation of iodide to iodine. The addition of iodine to matrix has been demonstrated to greatly facilitate the MALDI-TOF MS process and is a valuable tool when complex protein mixtures need to be analyzed.

scholarly journals A Moldy Application of MALDI: MALDI-ToF Mass Spectrometry for Fungal Identification

2019 ◽  
Vol 5 (1) ◽  
pp. 4 ◽  
Author(s):  
Robin Patel
Keyword(s):  
Mass Spectrometry ◽  
Filamentous Fungi ◽  
Bacterial Identification ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Fungal Identification ◽  
Routine Identification ◽  
Tof Ms

As a result of its being inexpensive, easy to perform, fast and accurate, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-ToF MS) is quickly becoming the standard means of bacterial identification from cultures in clinical microbiology laboratories. Its adoption for routine identification of yeasts and even dimorphic and filamentous fungi in cultures, while slower, is now being realized, with many of the same benefits as have been recognized on the bacterial side. In this review, the use of MALDI-ToF MS for identification of yeasts, and dimorphic and filamentous fungi grown in culture will be reviewed, with strengths and limitations addressed.

scholarly journals Use of matrix-assisted laser desorption ionization time-of-flight mass spectrometry analysis of serum peptidome to classify and predict COVID-19 severity

2021 ◽  
Author(s):  
Rosa M Gomila ◽  
Gabriel Martorell ◽  
Pablo A Fraile-Ribot ◽  
Antonio Doménech-Sánchez ◽  
Miguel Albertí ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Critical Patients ◽  
Tof Ms ◽  
Matrix Assisted Laser

Abstract Background Classification and early detection of severe COVID-19 patients is required to establish an effective treatment. We tested the utility of matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to classify and predict the severity of COVID-19. Methods We used MALDI-TOF MS to analyse the serum peptidome from 72 COVID-19 patients (training cohort), clinically classified as mild (28), severe (23) and critical (21), and 20 healthy controls. The resulting matrix of peak intensities was used for Machine Learning (ML) approaches to classify and predict COVID-19 severity of 22 independent patients (validation cohort). Finally, we analysed all sera by liquid chromatography mass spectrometry (LC MS/MS) to identify the most relevant proteins associated to disease severity. Results We found a clear variability of the serum peptidome profile depending on COVID-19 severity. Forty-two peaks exhibited a log fold change ≥ 1 and 17 were significantly different and at least four-fold more intense in the set of critical patients than in the mild ones. ML approach classified clinical stable patients according to their severity with a 100% of accuracy and predicted correctly the evolution of the non-stable patients in all cases. LC MS/MS identified five proteins that were significantly upregulated in the critical patients. They included the serum amyloid protein A2, which probably yielded the most intense peak detected by MALDI-TOF MS. Conclusion We demonstrated the potential of the MALDI-TOF MS as a bench to bedside technology to aid clinicians in their decisions on COVID-19 patients.

Limits for the detection of (poly-)phosphoinositides by matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS)

2001 ◽  
Vol 110 (2) ◽  
pp. 151-164 ◽  
Author(s):  
Matthias Müller ◽  
Jürgen Schiller ◽  
Marijana Petković ◽  
Wolf Oehrl ◽  
Regina Heinze ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Time Of Flight ◽  
Laser Desorption ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Tof Ms ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

CsCl as an auxiliary reagent for the analysis of phosphatidylcholine mixtures by matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS)

2001 ◽  
Vol 113 (1-2) ◽  
pp. 123-131 ◽  
Author(s):  
Jürgen Schiller ◽  
Rosmarie Süß ◽  
Marijana Petković ◽  
Nicole Hilbert ◽  
Matthias Müller ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Time Of Flight ◽  
Laser Desorption ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Tof Ms ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

scholarly journals Τυποποίηση στελεχών Legionella Pneumophila από περιβαλλοντικά δείγματα και συρροές κρουσμάτων με Matrix assisted laser desortion ionization Time-of-Flight Mass Spectrometry (ΜALDI-TΟF MS)

2019 ◽  
Author(s):  
Μαρία Κυρίτση
Keyword(s):  
Mass Spectrometry ◽  
Monoclonal Antibodies ◽  
Legionella Pneumophila ◽  
Time Of Flight ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Tof Ms ◽  
Matrix Assisted Laser

Η εκτίμηση κινδύνου και η πρόληψη της Νόσου των Λεγεωναρίων βασίζονται κυρίως στην ανίχνευση του μικροοργανισμού σε δείγματα νερού. Νόσο στον άνθρωπο προκαλεί κυρίως η Legionella pneumophila οροτύπου 1 (Lp1) και ιδιαίτερα στελέχη τα οποία φέρουν τις περιοχές παθογονικότητας lvh και rtxA. Επομένως, η αξιολόγηση τόσο του ορότυπου όσο και των προαναφερθέντων παραγόντων παθογονικότητας είναι εξαιρετικά σημαντική προκειμένου να υπάρχει ολοκληρωμένη εκτίμηση κινδύνου ενός συστήματος νερού. Σκοπός της διατριβής: 1. Η ανάπτυξη μίας απλής, ταχείας, φθηνής και αξιόπιστης μεθοδολογίας για τον προσδιορισμό των οροτύπων στελεχών Lp, η οποία να μπορεί να εφαρμοστεί ως μέθοδος ρουτίνας σε ένα μικροβιολογικό εργαστήριο.2. Ο προσδιορισμός αξιόπιστων βιοδεικτών με σκοπό την ανίχνευση των περιοχών παθογονικότητας lvh και rtxA σε στελέχη τα οποία απομονώνονται κατά τη διάρκεια τακτικών ελέγχων. 3. Η επικύρωση των ανωτέρω μεθόδων. Υλικά και μέθοδοι: Για τη διατριβή χρησιμοποιήθηκαν 15 στελέχη αναφοράς και 150 περιβαλλοντικά στελέχη Lp (70 Lp1 και 80 Lp2-15 στελέχη). Οκτώ (8) Lp2-15 στελέχη οροτυπήθηκαν με τη χρήση μονοκλωνικών αντισωμάτων (monoclonal antibodies-Mab), ενώ τα υπόλοιπα περιβαλλοντικά στελέχη οροτυπήθηκαν με τη χρήση δοκιμασίας ανοσοσυγκόλησης. Σε όλα τα στελέχη πραγματοποιήθηκε PCR προς ανίχνευση των περιοχών lvh και rtxA. Κατόπιν, δημιουργήθηκαν ομάδες στελεχών αναφοράς και στελεχών επικύρωσης. Όλα τα στελέχη υποβλήθηκαν σε εξαγωγή των ριβοσωμικών και επιφανειακών πρωτεϊνών και ακολούθησε ανάλυση με MALDI-TOF MS. Στα πρωτογενή φάσματα των στελεχών των ομάδων αναφοράς έγινε επεξεργασία με το λογισμικό Mass-Up για την ανάδειξη βιοδεικτών τόσο για τον προσδιορισμό του οροτύπου όσο και για την ανίχνευση των περιοχών παθογονικότητας. Ακολούθησε επικύρωση της μεθόδου με τη χρήση των στελεχών των ομάδων επικύρωσης.Αποτελέσματα: Για τον προσδιορισμό του οροτύπου η ανάλυση με το λογισμικό Mass-up υπέδειξε πέντε (5) πρωτεϊνικές κορυφές ως πιθανούς βιοδείκτες για τον ορότυπο. Ο αλγόριθμος ταξινόμησε ορθά 115 από τα 132 στελέχη της ομάδας επικύρωσης με ακρίβεια 87,12% (95%CI:80,18-92,32%). Η ευαισθησία της μεθόδου προσδιορίστηκε στο 87,5% (95%CI:77.59-94.12%) και η ειδικότητα στο 86,67% (95%CI:75,41-94,06%). Σχετικά με την ανίχνευση των περιοχών παθογονικότητας προσδιορίστηκαν δύο πρωτεϊνικές κορυφές που μπορούν να χρησιμεύσουν ως βιοδείκτες για την rtxA περιοχή και μία για την lvh. Σε ό,τι αφορά την ανίχνευση της lvh περιοχής, με τη χρήση των βιοδεικτών, ταξινομήθηκαν ορθά τα 113 από τα 137 θετικά και όλα τα αρνητικά στελέχη (14 συνολικά). Η μέθοδος επέδειξε ευαισθησία 82,48% (95%CI:75,06-88,44%), ειδικότητα 100,00% (95%CI:76,84-100,00%) και ακρίβεια 84,11% (95%CI:77,28-89,54%). Ομοίως, για την rtxA περιοχή, 134 από τα 134 θετικά στελέχη και 14 από τα 17 αρνητικά στελέχη ταξινομήθηκαν ορθά. Η μέθοδος παρουσίασε ευαισθησία 100,00% (95%CI:97,28-100,00%), ειδικότητα 76,47% (95%CI:50,10-93,19%) και ακρίβεια 97,35% (95%CI:93,36-99,27%). Συμπεράσματα: Η μέθοδος MALDI-TOF MS, με τη χρήση του πρωτοκόλλου της πρωτεϊνικής εξαγωγής, αποδείχθηκε αξιόπιστη τόσο στον προσδιορισμό του οροτύπου της Lp όσο και στην ανίχνευση των περιοχών παθογονικότητας lvh και rtxA. Αυτοματοποίηση της μεθόδου και περαιτέρω αξιολόγηση με τη χρήση της τεχνικής της απευθείας επίστρωσης του μικροοργανισμού, ώστε να απλοποιηθεί ακόμη περισσότερο το πρωτόκολλο, θα μπορούσε να συνεισφέρει σε μεγαλύτερο βαθμό στην ταχεία, φθηνή και απλή αξιολόγηση κινδύνου, καθώς και στη διερεύνηση κρουσμάτων της Νόσου των Λεγεωναρίων.

scholarly journals Evaluation of MALDI-TOF Mass Spectrometry and Sepsityper Kit™ for the Direct Identification of Organisms from Sterile Body Fluids in a Canadian Pediatric Hospital

2013 ◽  
Vol 24 (4) ◽  
pp. 191-194 ◽  
Author(s):  
Manal Tadros ◽  
Astrid Petrich
Keyword(s):  
Mass Spectrometry ◽  
Blood Culture ◽  
Software Tool ◽  
Rapid Identification ◽  
Correct Identification ◽  
Direct Identification ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Tof Ms

Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) can be used to identify bacteria directly from positive blood and sterile fluid cultures. The authors evaluated a commercially available kit – the Sepsityper Kit (Bruker Daltonik, Germany) – and MALDI-TOF MS for the rapid identification of organisms from 80 flagged positive blood culture broths, of which 73 (91.2%) were blood culture specimens and seven (8.7%) were cerebrospinal fluid specimens, in comparison with conventional identification methods. Correct identification to the genus and species levels was obtained in 75 of 80 (93.8%) and 39 of 50 (78%) blood culture broths, respectively. Applying the blood culture analysis module, a newly developed software tool, improved the species identification of Gram-negative organisms from 94.7% to 100% and of Gram-positive organisms from 66.7% to 70%.MALDI-TOF MS is a promising tool for the direct identification of organisms cultured from sterile sites.

scholarly journals A New Filter Based Cultivation Approach for Improving Aspergillus Identification using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS)

2022 ◽  
Author(s):  
Husam Salah ◽  
Anna Kolecka ◽  
Anna Rozaliyani ◽  
Retno Wahyuningsih ◽  
Saad J. Taj-Aldeen ◽  
...  
Keyword(s):  
Filamentous Fungi ◽  
Laser Desorption ◽  
Correct Identification ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Cultivation Method ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Tof Ms

AbstractMatrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) is widely used in clinical laboratories for routine identification of bacteria and yeasts. However, methodological difficulties are still apparent when applied to filamentous fungi. The liquid cultivation method recommended by Bruker Daltonics GmbH for identification of filamentous fungi by MALDI-TOF MS is labour intensive and time-consuming. In this study, growth of Aspergillus species on different (porous) surfaces was investigated with the aim to develop a more reliable, quicker and less laborious identification method using MALDI-TOF MS. Mycelial growth without sporulation mimicking liquid cultivation and reliable MALDI-TOF MS spectra were obtained when A. fumigatus strains were grown on and in between a polycarbonate membrane filter on Sabouraud dextrose agar. A database of in-house reference spectra was created by growing Aspergillus reference strains (mainly focusing on sections Fumigati and Flavi) under these selected conditions. A test set of 50 molecularly identified strains grown under different conditions was used to select the best growth condition for identification and to perform an initial validation of the in-house database. Based on these results, the cultivation method on top of a polycarbonate filter proved to be most successful for species identification. This method was therefore selected for the identification of two sets of clinical isolates that mainly consisted of Aspergilli (100 strains originating from Indonesia, 70 isolates from Qatar). The results showed that this cultivation method is reliable for identification of clinically relevant Aspergillus species, with 67% and 76% correct identification of strains from Indonesia and Qatar, respectively. In conclusion, cultivation of Aspergilli on top of a polycarbonate filter showed improved results compared to the liquid cultivation protocol recommended by Bruker in terms of percentage of correct identification, ease of MSP creation, time consumption, cost and labour intensity. This method can be reliably applied for identification of clinically important Aspergilli and has potential for identification of other filamentous fungi.

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