Acute Appendicitis Associated with Hantaan Virus Infection

2021 ◽  
Author(s):  
Sung-Chul Lim ◽  
Young Min Lee ◽  
Choon-Mee Kim ◽  
Na Ra Yun ◽  
Dong-Min Kim
Keyword(s):  
Virus Infection ◽  
Peripheral Nerve ◽  
Hemorrhagic Fever ◽  
Hantaan Virus ◽  
Nerve Bundle ◽  
Rt Pcr ◽  
Antibody Assay ◽  
Immunofluorescent Antibody ◽  
Virus Antigens ◽  
Indirect Immunofluorescent

Hantaviruses are Bunyaviridae viruses that cause hemorrhagic fever with renal syndrome (HFRS). Appendicitis caused by Hantaan virus has not been reported previously. An 81-year-old man who underwent laparoscopic appendectomy for suspected appendicitis based on abdominal pain, fever, hypotension, and computed tomography findings. Based on a suspicion of hemorrhagic fever with renal syndrome, the patient’s plasma was simultaneously analyzed using an indirect immunofluorescent antibody assay and nested reverse transcription–polymerase chain reaction (RT-PCR). The appendix tissue was also analyzed using nested RT-PCR and immunohistochemical (IHC) staining to identify the presence of Hantaan virus. Nested RT-PCR detected the presence of Hantaan virus, and indirect immunofluorescent antibody assay results revealed the presence of elevated antibody levels. Furthermore, IHC staining of the appendix tissue confirmed Hantaan virus antigens in the peripheral nerve bundle. Based on these findings, we confirmed the nerve tropism of the Hantaan virus. Hantaan virus in plasma and appendix tissue samples was confirmed using PCR and phylogenetic tree analysis. Moreover, we detected hypertrophy of the submucosa and periappendiceal adipose tissue nerve bundle along with Hantaan virus antigens in peripheral nerve bundles using IHC staining. Hence, we report that Hantaan virus infection may be accompanied by appendicitis.

scholarly journals Indirect ELISA and Indirect Immunofluorescent Antibody Assay for Detecting the Antibody against Murine Norovirus S7 in Mice

2010 ◽  
Vol 59 (1) ◽  
pp. 47-55 ◽  
Author(s):  
Yota KITAGAWA ◽  
Yukinobu TOHYA ◽  
Fumio IKE ◽  
Ayako KAJITA ◽  
Sang-Jin PARK ◽  
...  
Keyword(s):  
Indirect Elisa ◽  
Murine Norovirus ◽  
Antibody Assay ◽  
Immunofluorescent Antibody ◽  
Indirect Immunofluorescent

scholarly journals Simultaneous rapid detection of Hantaan virus and Seoul virus using RT-LAMP in rats

PeerJ ◽  
2019 ◽  
Vol 6 ◽  
pp. e6068 ◽  
Author(s):  
Xin Sui ◽  
Xu Zhang ◽  
Dongliang Fei ◽  
Zhen Zhang ◽  
Mingxiao Ma
Keyword(s):  
Rapid Detection ◽  
Hepatitis Virus ◽  
Lamp Assay ◽  
Cost Effective ◽  
Hemorrhagic Fever ◽  
Hantaan Virus ◽  
Rt Pcr ◽  
Monitoring Method ◽  
Clinical Specimens ◽  
Seoul Virus

Background Hemorrhagic fever with renal syndrome is in most cases caused by the Hantaan virus (HTNV) and Seoul virus (SEOV). To develop and apply reverse transcription loop-mediated isothermal amplification (RT-LAMP) to detect HTNV and SEOV simultaneously, which was faster, more cost effective, and easier to perform as the target gene amplified rapidly. In this article an assay based on LAMP is demonstrated, which only employs such apparatus as a water bath or a heat block. Methods A chromogenic method using the calcein/Mn2+ complex and real-time turbidity monitoring method were used to assess reaction progress of the reaction, and the specificity of the RT-LAMP-based assay was assessed by detecting cDNAs/cRNAs generated from Coxsackievirus A16, Influenza virus, lymphocytic choriomeningitis virus, mouse poxvirus, rotavirus, mouse hepatitis virus. In addition, 23 clinical specimens were used to determine the agreement between the RT-LAMP assay with reverse transcriptase polymerase chain reaction (RT-PCR) and immunofluorescence (IFT) method. Results The detection limit of RT-LAMP to HNTV and SEOV was as low as 10 copies/μL with optimized reaction conditions, which was much more sensitive than the RT-PCR method (100–1,000 copies/μL). At the same time, the detection results of 23 clinical specimens have also illustrated the agreement between this the RT-LAMP assay with RT-PCR and IFT. Discussion This RT-LAMP assay could be used to perform simultaneous and rapid detection of HTNV and SEOV to the clinical specimens.

Growth Experiment of Hantaan Virus in A549 Cells: An Attempt to Improve the Immunofluorescent Antibody Technique for Hemorrhagic Fever with Renal Syndrome

1986 ◽  
Vol 35 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Chuya HAMADA ◽  
Norimitsu L. SATO ◽  
Sueo NIIMURA ◽  
Akiko KATO ◽  
Nobuyoshi FUJISAWA ◽  
...  
Keyword(s):  
A549 Cells ◽  
Hemorrhagic Fever ◽  
Hantaan Virus ◽  
Growth Experiment ◽  
Antibody Technique ◽  
Immunofluorescent Antibody

Positive serological evidence that Hantaan virus, the etiologic agent of hemorrhagic fever with renal syndrome, is endemic in Canada

1984 ◽  
Vol 30 (9) ◽  
pp. 1137-1140 ◽  
Author(s):  
Ho Wang Lee ◽  
In Wha Seong ◽  
Luck Ju Baek ◽  
Donald A. McLeod ◽  
Jeong Sun Seo ◽  
...  
Keyword(s):  
Age Groups ◽  
Hemorrhagic Fever ◽  
Etiologic Agent ◽  
National Average ◽  
Hantaan Virus ◽  
Serological Evidence ◽  
Atlantic Region ◽  
Antibody Technique ◽  
The World ◽  
Indirect Immunofluorescent

Hemorrhagic fever with renal syndrome is endemic in most parts of the world. The etiological agent in known as Hantaan virus. More than 2000 Canadian sera from all age groups and provinces were tested for the presence of antibody to this virus by the indirect immunofluorescent antibody technique. The frequency of seropositivity ranged from 0.3 to 4.0%, with the national average being 1.4%. Newfoundland and New Brunswick in the Atlantic region had the highest positivity, being higher than hyperendemic areas in Korea. The inland provinces of Ontario, Manitoba, and Alberta had only 0.5% positives to Hantaan virus. In contrast, approximately 1.5% of sera from Saskatchewan, Quebec, and British Columbia were positive. No correlation in positivity was found between geography or sex, but positivity did increase with age. The highest titres were found in the group aged 65 to 93 years.

Hemorrhagic fever with renal syndrome caused by Hantaan virus infection in four pregnant Chinese women

2017 ◽  
Vol 89 (10) ◽  
pp. 1865-1870 ◽  
Author(s):  
Fanpu Ji ◽  
Wenxuan Zhao ◽  
Hailing Liu ◽  
Hongbo Zheng ◽  
Shengbang Wang ◽  
...  
Keyword(s):  
Virus Infection ◽  
Chinese Women ◽  
Hemorrhagic Fever ◽  
Hantaan Virus

Cytokine response to Hantaan virus infection in patients with hemorrhagic fever with renal syndrome

2017 ◽  
Vol 89 (7) ◽  
pp. 1139-1145 ◽  
Author(s):  
Jing Guo ◽  
Xuli Guo ◽  
Yong Wang ◽  
Fang Tian ◽  
Weiguang Luo ◽  
...  
Keyword(s):  
Virus Infection ◽  
Hemorrhagic Fever ◽  
Cytokine Response ◽  
Hantaan Virus

scholarly journals A Case Report of Empty Sella Syndrome secondary to Hantaan Virus Infection and Review of the Literature

2019 ◽  
Author(s):  
Lixin Lou ◽  
Peng Zhang ◽  
Bin Wang ◽  
Yang Wang
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Virus Infection ◽  
Anterior Pituitary ◽  
Hemorrhagic Fever ◽  
Empty Sella ◽  
Pituitary Function ◽  
Hantaan Virus ◽  
Resonance Imaging ◽  
Empty Sella Syndrome

Abstract Background Bleeding in the anterior pituitary lobe leading to tissue necrosis occurs in acute stage of severe clinical forms of hemorrhagic fever with renal syndrome (HFRS), while atrophy of the anterior pituitary lobe with diminution of the gland function occurs after recovery stage. The relationship between hantaan virus infection and empty sella syndrome (EES) was reported rarely. Case presentation We herein report a case of EES secondary to hantaan virus infection. This patient was a 54-year-old previously healthy Chinese male. He presented with fever, headache and backache with dizziness and oliguria. Physical examination was notable for hypotention and the signs of conjunctival suffusion. His platelet dropped and the urine protein was positive. The IgM to Hantaan virus and virus RNA were positive. Then he was diagnosed as hemorrhagic fever with renal syndrome. But in his diuretic phase, his 24-hour urine volume was still kept on the level of 10, 000 ml and his blood pressure was obviously higher for a week. Then the examination of pituitary function and brain magnetic resonance imaging were performed. The levels of the hormones were much lower and the magnetic resonance imaging results indicated empty sella. The patient’s symptoms were improved and he was discharged from the hospital soon after hormone replacement therapy. Conclusions The examination of pituitary function and MRI of brain need to be considered to scan ESS and panhypopituitarism in the patients with HFRS accompanied with diabetes insipidus.

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