scholarly journals Effect of aqueous extract of Brysocarpus coccineus (Connaraceae) on oxidative stress biomarkers in isoniazid induced oxidative stress in adult male wistar rats

2017 ◽  
Vol 10 (1) ◽  
pp. 51
Author(s):  
K Andrew ◽  
F.A. Dawud ◽  
J Yau
Keyword(s):  
Oxidative Stress ◽  
Aqueous Extract ◽  
Adult Male ◽  
Wistar Rats ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Male Wistar Rats

scholarly journals Effects of Aqueous Leaf Extract of Simarouba glauca DC (Simaroubaceae) on Lipoprotein Homeostasis and Oxidative Stress Biomarkers

2021 ◽  
Vol 1 (1) ◽  
pp. 20-29
Author(s):  
Sammydavies E. Osagie-Eweka ◽  
Noghayin J. Orhue ◽  
Eric I. Omogbai
Keyword(s):  
Oxidative Stress ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Biologically Active ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Male Wistar Rats ◽  
Aqueous Leaf Extract ◽  
Simarouba Glauca ◽  
And Oxidative Stress

Background and Purpose: Simarouba glauca is widely reported to contain a number of biologically active compounds with potentials in the treatment of numerous diseases. The study was conducted to evaluate the sub-acute effects of the aqueous leaf extract of Simarouba glauca (AESG) on lipoproteins and oxidative stress biomarkers in male Wistar rats. Methods: Oral administration of AESG was carried out in line with the guidelines of the Organization for Economic Co-operation and Development (OECD), No. 425 using a total of 24 male Wistar rats allotted to four groups (n=6); given distilled water, 500, 1000, and 2000 mg/kg/day of AESG respectively for 30 days. Results: In plasma, there was a significant reduction (P?0.05) in HDL-cholesterol; elevated (P?0.05) triglycerides (TG) at 1000 and 2000 mg/kg/day; elevated (P?0.05), and LDL-cholesterol at 500 and 1000 mg/kg/day, relative to the control. While the level of liver total cholesterol (TC) reduced significantly, it increased in the heart. Catalase (CAT) activity in the liver increased significantly (P?0.05) at all doses. The dose of 1000 mg/kg/day significantly (P?0.05) elevated kidney CAT activity. The activities of superoxide dismutase (SOD) in liver and heart reduced (P?0.05) at 500 mg/kg/day. At all doses, the levels of reduced glutathione (GSH) in plasma, liver and heart were comparable with the control. Although, there were no significant changes in plasma and liver glutathione peroxidase (GSH-PX) activity at all doses, animals given 500 mg/kg had reduction (P?0.05) in the heart GSH-PX activity compared to the control. Conclusion: Oral sub-acute AESG at high doses altered lipid homeostasis in plasma and heart without lipid peroxidation or oxidative stress. The extract has the potential to cause hyperlipidemia.

scholarly journals Effect of sodium (S)-2-hydroxyglutarate in male, and succinic acid in female Wistar rats against renal ischemia-reperfusion injury, suggesting a role of the HIF-1 pathway

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9438
Author(s):  
Eduardo Cienfuegos-Pecina ◽  
Tannya R. Ibarra-Rivera ◽  
Alma L. Saucedo ◽  
Luis A. Ramírez-Martínez ◽  
Deanna Esquivel-Figueroa ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Succinic Acid ◽  
Wistar Rats ◽  
Ischemia Reperfusion ◽  
The Other ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Female Wistar Rats ◽  
Dose Dependent ◽  
Nephroprotective Effect

Background Ischemia–reperfusion (IR) injury is the main cause of delayed graft function in solid organ transplantation. Hypoxia-inducible factors (HIFs) control the expression of genes related to preconditioning against IR injury. During normoxia, HIF-α subunits are marked for degradation by the egg-laying defective nine homolog (EGLN) family of prolyl-4-hydroxylases. The inhibition of EGLN stabilizes HIFs and protects against IR injury. The aim of this study was to determine whether the EGLN inhibitors sodium (S)-2-hydroxyglutarate [(S)-2HG] and succinic acid (SA) have a nephroprotective effect against renal IR injury in Wistar rats. Methods (S)-2HG was synthesized in a 22.96% yield from commercially available L-glutamic acid in a two-step methodology (diazotization/alkaline hydrolysis), and its structure was confirmed by nuclear magnetic resonance and polarimetry. SA was acquired commercially. (S)-2HG and SA were independently evaluated in male and female Wistar rats respectively after renal IR injury. Rats were divided into the following groups: sham (SH), nontoxicity [(S)-2HG: 12.5 or 25 mg/kg; SA: 12.5, 25, or 50 mg/kg], IR, and compound+IR [(S)-2HG: 12.5 or 25 mg/kg; SA: 12.5, 25, or 50 mg/kg]; independent SH and IR groups were used for each assessed compound. Markers of kidney injury (BUN, creatinine, glucose, and uric acid) and liver function (ALT, AST, ALP, LDH, serum proteins, and albumin), proinflammatory cytokines (IL-1β, IL-6, and TNF-α), oxidative stress biomarkers (malondialdehyde and superoxide dismutase), and histological parameters (tubular necrosis, acidophilic casts, and vascular congestion) were assessed. Tissue HIF-1α was measured by ELISA and Western blot, and the expression of Hmox1 was assessed by RT-qPCR. Results (S)-2HG had a dose-dependent nephroprotective effect, as evidenced by a significant reduction in the changes in the BUN, creatinine, ALP, AST, and LDH levels compared with the IR group. Tissue HIF-1α was only increased in the IR group compared to SH; however, (S)-2HG caused a significant increase in the expression of Hmox1, suggesting an early accumulation of HIF-1α in the (S)-2HG-treated groups. There were no significant effects on the other biomarkers. SA did not show a nephroprotective effect; the only changes were a decrease in creatinine level at 12.5 mg/kg and increased IR injury at 50 mg/kg. There were no effects on the other biochemical, proinflammatory, or oxidative stress biomarkers. Conclusion None of the compounds were hepatotoxic at the tested doses. (S)-2HG showed a dose-dependent nephroprotective effect at the evaluated doses, which involved an increase in the expression of Hmox1, suggesting stabilization of HIF-1α. SA did not show a nephroprotective effect but tended to increase IR injury when given at high doses.

Chronic consumption of Hypericum humifusum leaf extracts impairs epididymis spermatozoa characters in association with oxidative stress in adult male Wistar rats

2017 ◽  
Vol 93 ◽  
pp. 616-625 ◽  
Author(s):  
Imen Hammami ◽  
Ridha Ben Ali ◽  
Afef Nahdi ◽  
Olfa Kallech-Ziri ◽  
Marwa Boussada ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Adult Male ◽  
Wistar Rats ◽  
Leaf Extracts ◽  
Male Wistar Rats

TiO2 Nanoparticles Induced Oxidative Stress Mediated DNA Damage In The Liver Of Adult Male Wistar Rats

2018 ◽  
Vol 10 (2) ◽  
pp. 145-150
Author(s):  
Dasal Vasantharaja ◽  
◽  
Venugopal Ramalingam ◽  
Shanmugam Thangapandiyan ◽  
Nagarajan Sridhar ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Tio2 Nanoparticles ◽  
Adult Male ◽  
Wistar Rats ◽  
Male Wistar Rats
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