Effect Of Culture Media On The Plant Growth And Establishment Of Myrianthus arboreous (P. beauv) vegetative propagule

Marumi kumquat (Fortunella Japonica) is culture for its valuable nutritional value and medicinal importance in many regions of the world. The current study aimed to evaluate the effect of two types of media enriched with different concentrations of fructose and different plant growth regulators and different fructose concentration on in vitro propagation of Fortunella Japonica. The findings showed that the most effective treatment for explant surface sterilization was by using 0.1% HgCl2 for ten minutes which give best results for production contamination-free explants at the initiation cultures. At multiplication stage, WPM medium gave better results at all tested BA levels as compared with MS medium. No significant differences were showed by using BA alone or in combination with GA3 in the measured parameters. It has been observed that WPM medium supplemented with 0.5mgl-1 BA with the presence of 30mgl-1 fructose was able to give the highest shoot length (1.56cm) with maximum shoots number/explant 9.0 and highest leaves number/explant (21.0). The proliferated shoots were exposed to full strength MS medium salts supplemented with 2mgl-1 NAA which showed the highest ratio of rooting. In vitro rooted plantlets were gradually acclimatized and transferred to open air conditions, which recorded a high survive rate reached to 92%

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Effect of plant growth-promoting rhizobacterial composite culture on the growth of chickpea seedlings

Biologija ◽

10.6001/biologija.v64i2.3736 ◽

2018 ◽

Vol 64 (2) ◽

Cited By ~ 1

Author(s):

Jitendra Nag ◽

Biplab Dash ◽

Anup Kumar Singh ◽

Tapas Chowdhury ◽

Shyam Bihari Gupta ◽

...

Keyword(s):

Plant Growth ◽

Mixed Culture ◽

Culture Media ◽

Culture Collection ◽

Pot Experiment ◽

Seedling Test ◽

Departmental Culture ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Plant Growth Promoting Traits

In the present study, 20 soil and plant samples from 13 villages of Raipur, Durg, and Balod District of Chhattisgarh (India) were collected from chickpea fields. From these samples, a total of 86 isolates including 16 Rhizobium, 40 Azotobacter, 29 Azosprillum, and one PSB were obtained on selective culture media. All the isolates were screened for their plant growth-promoting traits. Three (GmR8, ASL3 & ASL4) out of 86 were finally selected for further studies. One Azotobacter isolate, i.e., Azo137, was selected from the departmental culture collection. Finally, four isolates including GmR8 (Rhizobium), ASL3, ASL4 (Azospirillum), and Azo137 were selected for composite culture formulations. GmR and ASL4 were siderophore-producing isolates, whereas ASL3 and Azo137 were IAA producer along with their ability to fix nitrogen. Five composite cultures were prepared randomly and tested for effect on the growth of chickpea (the seedling test and the pot experiment). Among all the composite culture groups, C2 (GmR8, Azo137, ASL4) significantly increased the root (10.84 cm) and shoot (8.10 cm) length, whereas biomass (3.60 g) was the highest in the case of C1 (GmR8, Azo137, ASL3, ASL4) of seedlings as compared to the control (6.80 cm, 2.60 cm, and 3.30 g, respectively). Overall, the study revealed a better performance of composite or mixed culture over individual bacteria.

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Propagação in vitro de Zingiber spectabile

Ornamental Horticulture ◽

10.14295/oh.v23i3.1035 ◽

2017 ◽

Vol 23 (3) ◽

pp. 270

Author(s):

Michele Valquíria Dos Reis ◽

Fernanda Carlota Nery ◽

Débora De Oliveira Prudente ◽

Patricia Duarte de Oliveira Paiva ◽

Renato Paiva ◽

...

Keyword(s):

Plant Growth ◽

Activated Charcoal ◽

In Vitro Propagation ◽

Culture Media ◽

In Vitro Germination ◽

Phase Pattern ◽

High Germination ◽

Length Width ◽

Ornamental Species

Zingiber spectabile is a tropical ornamental species with difficulties to obtain efficient propagation system. Thus, this study aimed to assess the in vitro propagation of Zingiber spectabile. Seed characterization was determined by measuring length, width and thickness, the weight of 1000 seeds and imbibition curve. In vitro germination of seeds was at constant (25 °C) or alternating temperatures (20-30 ºC). For optimization of in vitro multiplication, different concentrations of activated charcoal (0.0, 0.1 and 0.3%) and sucrose (0.0, 0.1, 0.3, 0.5 and 0.7 M) were evaluated. Plantlets were inoculated in flasks with different sealing systems (PVC covers with or without filters at the center) and culture media (MS or WPM). The plants were acclimatized in Plantmax® substrate. Seeds were of 6.06 mm length, 3.22 mm wide and 2.83 mm thick. The weight of 1,000 seeds corresponded to 46.4 g. The seed imbibition curve approaches to a tree phase pattern. Alternating temperatures induced high germination rates (68%). The addition of 0.3% activated charcoal provided higher root growth and plants with smaller number of senescent leaves. The best plant growth was obtained by the use of 0.1 M sucrose. All acclimatized plants survived (100%). The results demonstrate that Z. spectabile respond well to in vitro propagation.

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Amelioration of Saline Stress on Chia (Salvia hispanica L.) Seedlings Inoculated With Halotolerant Plant Growth-Promoting Bacteria Isolated From Hypersaline Environments

Frontiers in Agronomy ◽

10.3389/fagro.2021.665798 ◽

2021 ◽

Vol 3 ◽

Author(s):

María Florencia Yañez-Yazlle ◽

Neli Romano-Armada ◽

Verónica Beatriz Rajal ◽

Verónica Patricia Irazusta

Keyword(s):

Plant Growth ◽

Culture Media ◽

Saline Stress ◽

Plant Growth Promoting Bacteria ◽

Initial Growth ◽

Hypersaline Environments ◽

Salvia Hispanica ◽

Plant Growth Promoting ◽

Pgp Activities ◽

Growth Promoting

The rhizosphere and microbiome of halotolerant plants could be crucial for alleviating salinity stress during plant growth. The aims of this work were (1) to isolate bacteria from rhizosphere and bulk soil samples from the Salar del Hombre Muerto (Catamarca, Argentina), (2) to characterize different plant growth-promoting (PGP) activities produced by these bacterial isolates, and (3) to evaluate their effect on the initial growth of chia (Salvia hispanica L.) under saline stress. A total of 667 microorganisms were isolated, using different culture media with NaCl, and their abilities for nitrogen fixation, phosphate solubilization, siderophores production, and indole-3-acetic acid production were evaluated. Thirteen strains were selected for showing all the tested PGP activities; they belonged to the genera Kushneria, Halomonass, Pseudomonas, Planomicrobium, and Pseudarthrobacter. The strains Kushneria sp. and Halomonas sp. showed the highest salinity tolerance (from 50 to 2,000 mM NaCl) and biomass and biofilm production. Chia seeds were treated with six of the first 13 selected strains to evaluate their plant growth-promoting effect under saline stress (without and with 50 and 100 mM NaCl). Halomonas sp. 3R.12 and Kushneria sp. T3.7 produced heavier seedlings with a balanced shoot/root length ratio, while Pseudomonas sp. AN23 showed the best effect upon chia seedlings, with a morphological response similar to non-stressed seedlings. On the other hand, seedlings displayed no responses when inoculated with Planomicrobium sp. 3S.31 and Pseudarthrobacter sp. ER25. This study contributes to the knowledge on microorganisms from hypersaline environments as plant growth promoters for their use in the production of salt-sensitive crops, among other potential uses.

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THE ROLL OF SOME PLANT GROWTH REGULATORS ON SHOOTS MULTIPLICATION OF STEVIA PLANTS IN VITRO

IRAQI JOURNAL OF AGRICULTURAL SCIENCES ◽

10.36103/ijas.v48i5.322 ◽

2017 ◽

Vol 48 (5) ◽

Author(s):

Al-Obaidy & Khierallah

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Culture Media ◽

Stevia Rebaudiana ◽

Shoot Multiplication ◽

Dry Weight ◽

Fresh Weight ◽

Number Of Shoots

This research was conducted to study the effect of some plant growth regulators on in vitro shoots multiplication of stevia (Stevia rebaudiana Bertoni). The experiments included tests of various combinations of KIN with IBA or IAA in the shoot multiplication. Results indicated that KIN at 1.0 mg. L-1 plus 0.3 mg. L-1 of IBA produced the highest number of shoots (3.5 shoots) while KIN at 1.5 mg. L-1 plus IBA at 1.0 mg. L-1 produced the lowest shoot length (1.14 cm). Hormone free medium produced the highest rate of the leaves number reached 28.56 leaves. KIN and IBA interaction increased fresh and dry weight significantly. Treatment contained 2.0 mg -1 KIN plus 0.3 mg. L-1 IBA produced the highest fresh weight (1.739 g) while 0.5 mg. L-1 KIN and 0.3 mg. L-1 IBA produced the highest dry weight (0.822 g). As for the effect of interaction between the IAA and KIN it was significant in the number of shoots formed. Interaction between 1.0 mg. L-1 KIN with 0.1 mg. L-1IAA produced the highest number of shoots (3.8 shoots). Shoots length reached 8.10 cm in the media with 0.3 mg. L-1 IAA only. The highest fresh weight (1.267 g) was achieved with the interaction between 1.0 mg. L-1 KIN and 0.3 mg. L-1 IAA while 0.5 mg. L-1IAA without KIN produced the highest dry weight reached 0.138 g. Shoots multiplication was improved by incorporation of the cytokinin TDZ in culture media. Shoots number, fresh and dry weights were increased significantly by adding 0.05 mg. L-1 of TDZ at present of 0.3 mg. L-1 of IBA giving 6.6 shoots, 0.974 g and 0.144 g respectively while shoots length decreased significantly as media without TDZ produced the highest shoots length reached 9.32 cm. The above results can adopt for the successful in vitro shoot multiplication of Stevia plants.

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Effects of plant growth regulators, different culture media and strength MS on production of volatile fraction composition in shoot cultures of Ocimum basilicum

Industrial Crops and Products ◽

10.1016/j.indcrop.2018.02.075 ◽

2018 ◽

Vol 116 ◽

pp. 231-239 ◽

Cited By ~ 16

Author(s):

Lucila Elizabeth Fragoso Monfort ◽

Suzan Kelly Vilela Bertolucci ◽

Andreisa Fabri Lima ◽

Alexandre Alves de Carvalho ◽

Aliyu Mohammed ◽

...

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Culture Media ◽

Ocimum Basilicum ◽

Volatile Fraction ◽

Shoot Cultures ◽

Fraction Composition

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Bacteria Isolated from Wastewater Irrigated Agricultural Soils Adapt to Heavy Metal Toxicity While Maintaining Their Plant Growth Promoting Traits

Sustainability ◽

10.3390/su13147792 ◽

2021 ◽

Vol 13 (14) ◽

pp. 7792

Author(s):

Abdul Wahab Ajmal ◽

Saleha Saroosh ◽

Shah Mulk ◽

Muhammad Nadeem Hassan ◽

Humaira Yasmin ◽

...

Keyword(s):

Plant Growth ◽

Agricultural Soils ◽

Culture Media ◽

Growth Promotion ◽

Metal Resistance ◽

Rrna Gene ◽

Gene Sequence Analysis ◽

Plant Growth Promoting ◽

Growth Promoting

The present study explored the plant growth promotion and bioremediation potential of bacteria inhabiting wastewater irrigated agricultural soils. Thirty out of 75 bacterial isolates (40%), 29/75 (39%) and 28/75 (37%) solubilized Zn, K and PO4 during plate essays respectively. Fifty-six percent of the isolates produced siderophores, while 30% released protease in vitro. Seventy-four percent of bacteria resisted Pb, Ni and Cd at various concentrations added to the culture media plates. Sixteen out of 75 (26%) isolates were able to fix N in Nbf medium. Among these 16 N fixers, N fixing nifH, nifD and nifK genes was detected through PCR in 8, 7 and 1 strain respectively using gene specific primers designed in the study with Enterobacter sp. having all three (nifHKD) genes. Isolated bacteria showed resemblance to diverse genera such as Bacillus, Pseudomonas, Enterobacter, Citrobacter, Acinetobacter, Serratia, Klebsiella and Enterococcus based on 16S rRNA gene sequence analysis. In addition to showing the best mineral solubilization and metal resistance potential, Citrobacter sp. and Enterobacter sp. also removed 87%, 79% and 43% and 86%, 78% and 51% of Ni, Cd and Pb, respectively, from aqueous solution. These potent bacteria may be exploited both for bioremediation and biofertilization of wastewater irrigated soils leading to sustainable agriculture.

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Noxious effects of cell surface display glutamate sensors on plant growth and development

10.1101/2020.03.24.005223 ◽

2020 ◽

Cited By ~ 1

Author(s):

Vanessa Castro-Rodríguez ◽

Thomas J. Kleist ◽

Nicoline M. Gappel ◽

Sakiko Okumoto ◽

Mackenzie Machado ◽

...

Keyword(s):

Plant Growth ◽

Cell Surface ◽

Culture Media ◽

Lateral Roots ◽

Surface Display ◽

Cell Surface Display ◽

Quiescent Center ◽

Extracellular Glutamate ◽

Meristem Development ◽

Membrane Tethering

SummaryPlants use electrical and chemical signals for systemic communication. Herbivory, for instance, appears to trigger local apoplasmic glutamate accumulation, systemic electrical signals and calcium waves that travel to report insect damage to neighboring leaves and initiate defense. To monitor extra- and intracellular glutamate concentrations in plants, we generated lines expressing genetically encoded fluorescent glutamate sensors. In contrast to cytosolically localized sensors, extracellularly displayed variants inhibited plant growth and proper development. Phenotypic analyses of high-affinity display sensor lines revealed that root meristem development, particularly the quiescent center (QC), the number of lateral roots, vegetative growth and flower architecture were affected. Notably, the severity of the phenotypes was proportional to the affinity of the displayed glutamate sensors, intimating that their ability to bind extracellular glutamate caused the observed defects. Congruously, root growth defects were suppressed by supplementing culture media with low levels of glutamate. Overall, our data indicate sensor noxiousness was dependent on its membrane-tethering and likely caused by sequestration of extracellular glutamate, specifically at the cell surface, thereby either disrupting the supply of glutamate to meristematic cells and/or by impairing local glutamatergic signaling during development.

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