scholarly journals Propagação in vitro de Zingiber spectabile

2017 ◽  
Vol 23 (3) ◽  
pp. 270
Author(s):  
Michele Valquíria Dos Reis ◽  
Fernanda Carlota Nery ◽  
Débora De Oliveira Prudente ◽  
Patricia Duarte de Oliveira Paiva ◽  
Renato Paiva ◽  
...  
Keyword(s):  
Plant Growth ◽  
Activated Charcoal ◽  
In Vitro Propagation ◽  
Culture Media ◽  
In Vitro Germination ◽  
Phase Pattern ◽  
High Germination ◽  
Length Width ◽  
Ornamental Species

Zingiber spectabile is a tropical ornamental species with difficulties to obtain efficient propagation system. Thus, this study aimed to assess the in vitro propagation of Zingiber spectabile. Seed characterization was determined by measuring length, width and thickness, the weight of 1000 seeds and imbibition curve. In vitro germination of seeds was at constant (25 °C) or alternating temperatures (20-30 ºC). For optimization of in vitro multiplication, different concentrations of activated charcoal (0.0, 0.1 and 0.3%) and sucrose (0.0, 0.1, 0.3, 0.5 and 0.7 M) were evaluated. Plantlets were inoculated in flasks with different sealing systems (PVC covers with or without filters at the center) and culture media (MS or WPM). The plants were acclimatized in Plantmax® substrate. Seeds were of 6.06 mm length, 3.22 mm wide and 2.83 mm thick. The weight of 1,000 seeds corresponded to 46.4 g. The seed imbibition curve approaches to a tree phase pattern. Alternating temperatures induced high germination rates (68%). The addition of 0.3% activated charcoal provided higher root growth and plants with smaller number of senescent leaves. The best plant growth was obtained by the use of 0.1 M sucrose. All acclimatized plants survived (100%). The results demonstrate that Z. spectabile respond well to in vitro propagation.

scholarly journals The Response of in Vitro Propagation of Marumi Kumquat (fortunella Japonica Thunb.) to Different Culture Media, Plant Growth Regulators And Different Fructose Concentration

2020 ◽  
Vol 23 (1) ◽  
pp. 178-190
Author(s):  
Jeillan Hussein ◽  
Diaa ibraheam
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Nutritional Value ◽  
Culture Media ◽  
Shoot Length ◽  
Ms Medium ◽  
Surface Sterilization

Marumi kumquat (Fortunella Japonica) is culture for its valuable nutritional value and medicinal importance in many regions of the world. The current study aimed to evaluate the effect of two types of media enriched with different concentrations of fructose and different plant growth regulators and different fructose concentration on in vitro propagation of Fortunella Japonica. The findings showed that the most effective treatment for explant surface sterilization was by using 0.1% HgCl2 for ten minutes which give best results for production contamination-free explants at the initiation cultures. At multiplication stage, WPM medium gave better results at all tested BA levels as compared with MS medium. No significant differences were showed by using BA alone or in combination with GA3 in the measured parameters. It has been observed that WPM medium supplemented with 0.5mgl-1 BA with the presence of 30mgl-1 fructose was able to give the highest shoot length (1.56cm) with maximum shoots number/explant 9.0 and highest leaves number/explant (21.0). The proliferated shoots were exposed to full strength MS medium salts supplemented with 2mgl-1 NAA which showed the highest ratio of rooting. In vitro rooted plantlets were gradually acclimatized and transferred to open air conditions, which recorded a high survive rate reached to 92%

scholarly journals In vitro germination of Abricó-de-Macaco (Couroupita guianensis aubl.)zygotic embryos in different culture media and light spectra

2022 ◽  
Vol 52 (1) ◽  
Author(s):  
Christian Aparecido Demétrio ◽  
Jéssica Fernanda de Oliveira Jacob ◽  
Guilherme Bovi Ambrosano ◽  
Paulo Hercílio Viegas Rodrigues
Keyword(s):  
Plant Growth ◽  
Culture Media ◽  
Zygotic Embryos ◽  
In Vitro Germination ◽  
Forest Species ◽  
In Vitro Morphogenesis ◽  
Light Spectra ◽  
Couroupita Guianensis ◽  
Secondary Roots

ABSTRACT: Couroupita guianensis Aubl. is an Amazonian forest species with important medicinal and ornamental value. This study evaluated the effect of different culture media and light spectra on the in vitro germination and development of the zygotic embryos of C. guianensis. The culture media, MS and WPM, were evaluated without the addition of plant growth regulators and were associated with four LED light spectra: white (CW), 70% red + 30% blue (R2B), 100% red (R), and 100% blue (B). One hundred percent of the seeds successfully underwent in vitro germination, and the culture media did not interfere with embryo development. In addition to this, the different light spectra induced in vitro morphogenesis and R2B treatment significantly promoted the production of secondary roots. This effect may aid in the rooting and acclimatization of seedlings of this species.

scholarly journals Nodal segments and zygotic embryos in culture media for the in vitro propagation of tamarind

2019 ◽  
Vol 10 (4) ◽  
pp. 438-447
Author(s):  
Antonio Flávio Arruda Ferreira ◽  
Marcela Sant'anna Cordeiro da Silva ◽  
Laís Naiara Honorato Monteiro ◽  
Glaucia Amorim Faria ◽  
Aparecida Conceição Boliani ◽  
...  
Keyword(s):  
Activated Charcoal ◽  
In Vitro Propagation ◽  
Culture Media ◽  
Nodal Segments ◽  
Zygotic Embryos ◽  
Randomized Experimental Design ◽  
Long Time ◽  
Propagation Methods ◽  
Time Required

There has been a lack of research on the propagation methods of tamarind, hindering the availability and supply of its products. Considering the nutraceutical potential of tamarind and the long-time required to initiate production, this study aimed to evaluate the establishment of nodal segments and the germination of in vitro zygotic embryos of tamarind plants in culture media, salt concentrations, and activated charcoal, aiming at the establishment of an in vitro propagation protocol. For this purpose, MS and WPM media with 25, 50, 75 and 100% salt concentrations were used both with and without activated charcoal (2 g L-1) for the inoculation of nodal segments and zygotic embryos of tamarind. The experiment was conducted in a completely randomized experimental design, in a 2 x 4 x 2 factorial scheme (culture media x salt concentrations x activated charcoal) with 20 replications. It was observed that the in vitro establishment of tamarind nodal segments is viable when using the MS medium with 75% of salts plus 2 g L-1 of activated charcoal and that for the germination of zygotic embryos there was no influence of the treatments. It was also observed that in order to increase the length of the formed plantlets, the MS and WPM media can be employed with 75% of salts.

Control of browning during seed germination of Meizotropis pellita: a rare and an endangered plant

2021 ◽  
Vol 16 (8) ◽  
pp. 118-120
Author(s):  
Renu Chandola ◽  
Anshulika Upadhyaya
Keyword(s):  
Seed Germination ◽  
Culture Media ◽  
Light Condition ◽  
Endangered Plant ◽  
In Vitro Germination ◽  
Light Conditions ◽  
Low Intensity ◽  
Browning Reaction ◽  
Threatened Plant Species

Meizotropis pellita is a rare, endangered and threatened plant species found in Patwadanger forest. In nature this species grows from rootstock in precise and sensitive habitats. However, phenolic compounds provoke browning reaction which is responsible for lethal browning in plant tissue culture and hindering in vitro germination. The aim of this study was to minimize the effect of phenolic compound during seed germination. We evaluate different antibrowning treatments in Meizotropis pellita seeds. The seeds were pretreated in Polyvinylpyrrolidone and then treated seeds were cultured on MS media and sterilized seeds were directly inoculated on PVP containing MS media. These culture media incubate under dark and light conditions at different durations respectively. Our results showed that addition of antibrowning agent, PVP inhibit onset of browning. Dark and light condition also played a crucial role on onset of browning. Delayed and low intensity of browning were observed in case of seeds that were inoculated on MS media containing PVP and were kept in dark. The present research offers a positive in vitro seed germination protocol for Meizotropis pellita conservation.

scholarly journals ACTIVATED CHARCOAL APPLICATION FOR THE MICROPROPAGATION OF Cattleya crispata (Thunb.) Van den Berg

Nativa ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 352-358
Author(s):  
Denys Matheus Santana Costa Souza ◽  
Sérgio Bruno Fernandes ◽  
Letícia Vaz Molinari ◽  
Maria Lopes Martins Avelar ◽  
Gilvano Ebling Brondani
Keyword(s):  
Endemic Species ◽  
Activated Charcoal ◽  
Culture Medium ◽  
Genetic Conservation ◽  
Campo Rupestre ◽  
In Vitro Germination ◽  
Germination Speed

 Micropropagation is an alternative for the genetic conservation and propagation of endemic species from “Campo Rupestre Ferruginoso”, such as the orchid Cattleya crispata. The aim of the present study is to assess the influence of activated charcoal on the in vitro germination, multiplication and elongation phases of C. crispata. Seeds extracted from mature capsules were used for inoculation in the culture medium that was adopted to assess the effect of supplementation, or not, with activated charcoal. Data about germination speed, seedling number, length, vigor, oxidation and contamination (bacterial and/or fungal) were assessed through these phases. Based on the results obtained, the use of activated charcoal was efficient in the in vitro germination and multiplication phases of C. crispata, providing greater speed and percentage of germination, less contamination and oxidation of the tissues, greater number, length and vigor of shoots, being effective for the genetic conservation and production of seedlings of the species. Culture medium without the supplementation of activated charcoal provided the best results for the in vitro elongation, with greater length, vigor and less oxidation of shoots.

scholarly journals In vitro establishment and early development of barueiro (Dipteryx alata Vogel)

2016 ◽  
Vol 37 (4) ◽  
pp. 1779 ◽  
Author(s):  
Herick Fernando de Jesus Silva ◽  
Simone Abreu Asmar ◽  
Rayssa Camargo de Oliveira ◽  
Berildo De Melo ◽  
José Magno Queiroz Luz ◽  
...  
Keyword(s):  
Activated Charcoal ◽  
Large Scale ◽  
Culture Media ◽  
Germination Rate ◽  
Woody Species ◽  
Chlorophyll Contents ◽  
Fruit Species ◽  
In Vitro Establishment ◽  
Dipteryx Alata

The barueiro (Dipteryx alata Vog.) is a native fruit species of the Cerrado ecoregion that has multiple uses. It is a wild species, and its cultivation is difficult. Furthermore, it is threatened with extinction. Plant tissue culture is a major tool for the conservation of germplasm, as well as a means of propagating high-quality seedlings on a large scale. However, this technique has not been used with barueiro, although it might provide valuable contributions to the process of barueiro domestication. The most popular method of cultivation is the use of the Murashige and Skoog medium (MS), which is considered one of the most nutritionally complete media. Woody plant medium (WPM) is indicated for the propagation of woody species, but there are no reports of its use for barueiro cultivation. Woody plants tend to have problems with rust in vitro during the establishment phase. Activated charcoal acts as an adjuvant for the adsorption of phenolic compounds, mitigating its effects in the medium. Thus, the objective of this study was to test four activated charcoal doses (0, 2, 4 and 6 g L-1) and three culture media: MS, WPM, and AA (over water agar) in the in vitro establishment of barueiro. The experimental design was a completely randomised (DIC), 4 × 3 factorial design with three replications. At 60 days after inoculation, the explants were evaluated for dry matter, fresh weight, stem diameter, shoot length, number of leaves, longest root length, germination rate, and chlorophyll contents. The MS medium supplemented with 3,0 g L-1 activated charcoal appeared to be the best for in vitro establishment of barueiro.

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