scholarly journals Testing the infectivity of a Begomovirus by particle bombardment method using a gene gun

2019 ◽  
Vol 35 (2) ◽  
pp. 58
Author(s):  
A.L. Abubakar ◽  
M.M. Abarshi ◽  
M.N. Maruthi
Keyword(s):  
Particle Bombardment ◽  
Gene Gun

Transfection by particle bombardment: Delivery of plasmid DNA into mammalian cells using gene gun

2009 ◽  
Vol 1790 (8) ◽  
pp. 754-764 ◽  
Author(s):  
Masaki Uchida ◽  
Xiong Wei Li ◽  
Peter Mertens ◽  
H. Oya Alpar
Keyword(s):  
Particle Bombardment ◽  
Plasmid Dna ◽  
Mammalian Cells ◽  
Gene Gun

scholarly journals Cloning of SjCA gene and its expression analysis on upland cottons

2016 ◽  
Vol 2 (2) ◽  
pp. 150
Author(s):  
Jingjing Kong ◽  
Xuke Lu ◽  
Xiaojie Zhao ◽  
Zujun Yin ◽  
Shuai Wang ◽  
...  
Keyword(s):  
Transient Expression ◽  
Particle Bombardment ◽  
Expression System ◽  
Salt Resistance ◽  
Complete Sequence ◽  
Sequence Information ◽  
Gene Gun ◽  
Salt Tolerant ◽  
Germination Ability

Acquisition of salt-tolerant genes from exogenous plants to improve cotton salt resistance has always been a hotspot of research on cotton salt resistance. However, the information regarding the method of conversion of living cotton pollens by portable gene gun technology is still little. Complete sequence information of the Carbonic Anhydrase (CA) gene was obtained from NCBI database, and its full ORF length sequence was cloned by RT-PCR technology. After the construction of pBI121-CA::GFP fusion expression vector, we used cotton pollens from upland cotton varieties Y-2067, ZA-23, and GZ-2, which have weaker autofluorescence, to conduct the research on the transient expression of cotton pollens in vivo via particle bombardment (gene gun technology). The results indicated that green fluorescence enhancement of the three kinds of cotton pollens was realized after the CA gene transformation, meaning that the CA gene expression level was increased. Besides, the salt tolerance germination ability of transgenic T1 seeds was also improved. Our research initially established a transient expression system of cotton pollen in vivo via particle bombardment technology, laying the theoretical foundation for further research of cotton genetic transformation and creation of cotton salt-tolerant germplasm.

Gene Transfer via Particle Bombardment: Applications of the Accell Gene Gun

1994 ◽  
pp. 193-209 ◽  
Author(s):  
Ning-Sun Yang ◽  
Carolyn De Luna ◽  
Liang Cheng
Keyword(s):  
Gene Transfer ◽  
Particle Bombardment ◽  
Gene Gun

Construction and Use of a Simple Gene Gun for Particle Bombardment

2004 ◽  
pp. 265-272
Author(s):  
Dennis Gray ◽  
Michael Compton ◽  
Ernest Hiebert ◽  
Chia-Min Lin ◽  
Victor Gaba
Keyword(s):  
Particle Bombardment ◽  
Gene Gun

scholarly journals (283) Biolistic Genetic Transformation of Carica papaya L. using the Helios™ Gene Gun

HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 1053A-1053
Author(s):  
Salvador Guzmán-González ◽  
Pedro Valadez-Ramírez ◽  
Rosa-Edith Robles-Berber ◽  
Laura Silva-Rosales ◽  
José-Luis Cabrera-Ponce
Keyword(s):  
Genetic Transformation ◽  
Particle Bombardment ◽  
Carica Papaya ◽  
Resistance Mechanism ◽  
35S Promoter ◽  
Helium Pressure ◽  
Gene Gun ◽  
Gold Particle Size ◽  
Key Factor ◽  
Gus Transient Expression

Biolistic genetic transformation of plants with viral genes is a method for controlling plant virus diseases; however, optimization of the particle bombardment parameters according to the transformation system is a key factor for an appropiate transgene expression and, therefore, a stronger resistance mechanism in transgenic plants. In order to optimize biolistic parameters, somatic papaya (Carica papaya L.) cv. Maradol embryo masses were bombarded with the CAMBIA 1301 plasmid construction that contains the coat protein gene (CP) of the papaya ringspot virus isolate of Colima, Mexico, driven by the double constitutively CaMV 35S promoter and flanked for the GUS and hygromycin (hpt) resistance genes. Particle bombardment protocol was carried out using the Helios™ Gene Gun device (BioRad) and the manufacturer's instruction manual. Helium pressure (50, 100, and 150 psi) and gold particle size (0.6, 1.0, and 1.6 μm) were evaluated. Five days after bombardment, somatic embryo clusters were used for GUS transient expression and, during 2 months, were selected into 50, 75, and 150 mg·L-1 hygromycin-containing media to its later CP-PCR detection. Results showed that 50 psi and 1.0 μm were the two optimal values for the assayed analyses. This is the first report of genetic transformation of papaya using the Helios™ Gene Gun device as a new tool compared to conventional PDS-1000/He.

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