Gene Expression in Citrus Plant Cells Using Helios® Gene Gun System for Particle Bombardment

2019 ◽  
pp. 219-228
Author(s):  
Yosvanis Acanda ◽  
Chunxia Wang ◽  
Amit Levy
Keyword(s):  
Gene Expression ◽  
Particle Bombardment ◽  
Plant Cells ◽  
Gene Gun ◽  
Citrus Plant

scholarly journals Transient Expression of β-Glucuronidase (GUS) Activity in Embryogenic Callus Tissue of Alkaligrass (Puccinellia distans) using Particle Bombardment

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 913A-913
Author(s):  
Aaron Brown ◽  
Harrison G. Hughes
Keyword(s):  
Gene Expression ◽  
Transient Expression ◽  
Particle Bombardment ◽  
Callus Induction ◽  
Embryogenic Callus ◽  
Callus Tissue ◽  
Transient Gene Expression ◽  
Plant Cells ◽  
Gus Activity ◽  
Puccinellia Distans

Callus induction and regeneration of alkaligrass (Puccinellia distans) was developed in our laboratory for use in transformation studies of turfgrass. Particle bombardment of the embryogenic callus is being evaluated using a helium particle inflow gun constructed at Colorado State Univ., according to the design of Philippe et al. (Ohio State Univ., 1993). Its utility in delivering DNA to plant cells is being tested by measuring the frequency of transient gene expression of a reporter gene (GUS pBI121) in embryogenic callus of alkaligrass. Varying pressure of helium and the distance of the calli in the chamber are also being evaluated for efficiency in transformation.

Tracking Gene Expression in Plant Cells

2013 ◽  
pp. 277-310
Author(s):  
S. Bolte ◽  
Y. Boutté ◽  
S. Kluge ◽  
S. Brown ◽  
B. Satiat-Jeunemaître
Keyword(s):  
Gene Expression ◽  
Plant Cells

The Circadian Clock inArabidopsisRoots Is a Simplified Slave Version of the Clock in Shoots

Science ◽  
2008 ◽  
Vol 322 (5909) ◽  
pp. 1832-1835 ◽  
Author(s):  
Allan B. James ◽  
José A. Monreal ◽  
Gillian A. Nimmo ◽  
Ciarán L. Kelly ◽  
Pawel Herzyk ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Circadian Clock ◽  
Clock Genes ◽  
Plant Cells ◽  
Feedback Loops ◽  
Circadian Oscillator ◽  
Inhibit Gene Expression ◽  
Organ Specific ◽  
Plant Clock

The circadian oscillator in eukaryotes consists of several interlocking feedback loops through which the expression of clock genes is controlled. It is generally assumed that all plant cells contain essentially identical and cell-autonomous multiloop clocks. Here, we show that the circadian clock in the roots of matureArabidopsisplants differs markedly from that in the shoots and that the root clock is synchronized by a photosynthesis-related signal from the shoot. Two of the feedback loops of the plant circadian clock are disengaged in roots, because two key clock components, the transcription factors CCA1 and LHY, are able to inhibit gene expression in shoots but not in roots. Thus, the plant clock is organ-specific but not organ-autonomous.

Control of gene expression in plant cells using a 434:VP16 chimeric protein

1994 ◽  
Vol 24 (2) ◽  
pp. 381-388 ◽  
Author(s):  
Robin J. Wilde ◽  
Susan E. Cooke ◽  
William J. Brammar ◽  
Wolfgang Schuch
Keyword(s):  
Gene Expression ◽  
Plant Cells ◽  
Chimeric Protein ◽  
Control Of Gene Expression

Calreticulin, a multifunctional Ca2+ binding chaperone of the endoplasmic reticulum

1998 ◽  
Vol 76 (5) ◽  
pp. 779-785 ◽  
Author(s):  
Marek Michalak ◽  
Paola Mariani ◽  
Michal Opas
Keyword(s):  
Gene Expression ◽  
Endoplasmic Reticulum ◽  
Posttranslational Modification ◽  
Storage Capacity ◽  
Higher Plants ◽  
Plant Cells ◽  
Steroid Sensitive ◽  
Endoplasmic Reticulum Chaperone ◽  
Precise Role ◽  
Cellular Ca

Calreticulin is a ubiquitous endoplasmic reticulum Ca2+ binding chaperone. The protein has been implicated in a variety of diverse functions. Calreticulin is a lectin-like chaperone and, together with calnexin, it plays an important role in quality control during protein synthesis, folding, and posttranslational modification. Calreticulin binds Ca2+ and affects cellular Ca2+ homeostasis. The protein increases the Ca2+ storage capacity of the endoplasmic reticulum and modulates the function of endoplasmic reticulum Ca2+-ATPase. Calreticulin also plays a role in the control of cell adhesion and steroid-sensitive gene expression. Recently, the protein has been identified and characterized in higher plants but its precise role in plant cells awaits further investigation.Key words: calreticulin, endoplasmic reticulum, chaperone, Ca2+ binding protein.

scholarly journals In Vivo Analysis of Glucocorticoid-Induced Reporter Gene Expression Using Gene Gun DNA Delivery

2002 ◽  
Vol 25 (8) ◽  
pp. 1115-1118 ◽  
Author(s):  
Kiyoshi Tanigawa ◽  
Katsunao Tanaka ◽  
Hidetaka Nagase ◽  
Hidekazu Miyake ◽  
Mamoru Kiniwa ◽  
...  
Keyword(s):  
Gene Expression ◽  
Reporter Gene ◽  
Dna Delivery ◽  
Reporter Gene Expression ◽  
Gene Gun ◽  
In Vivo Analysis

scholarly journals Construction and screening of subtracted cDNA libraries from limited populations of plant cells: a comparative analysis of gene expression between maize egg cells and central cells

2005 ◽  
Vol 44 (1) ◽  
pp. 167-178 ◽  
Author(s):  
Quyên Lê ◽  
José F. Gutièrrez-Marcos ◽  
Liliana M. Costa ◽  
Stephanie Meyer ◽  
Hugh G. Dickinson ◽  
...  
Keyword(s):  
Gene Expression ◽  
Comparative Analysis ◽  
Plant Cells ◽  
Cdna Libraries ◽  
Subtracted Cdna Libraries
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