A rapid reversed-phase thin layer chromatographic detection protocol for adulteration in some edible fats and oils food formulation

2021 ◽  
Vol 16 (2) ◽  
pp. 48-51
Author(s):  
Mustapha Aliru Olajide ◽  
Adepoju Adenike Rashidat ◽  
Fadipe Victor Olugbenga
Keyword(s):  
Thin Layer ◽  
Vegetable Oil ◽  
Fats And Oils ◽  
Reversed Phase ◽  
Detection Methods ◽  
Blue Band ◽  
Edible Fats ◽  
Animal Fat ◽  
Chromatographic Detection ◽  
Salad Cream

The problems of adulteration in the vegetable oil and fat have been the major draw backs in the food products formulation, in spite of the various adulteration detection methods in different applications that have been reported. However, the detection tools that can be fast and reliable for the routine analysis necessitated the current work. The two groups of three different samples: vegetable fat containing sample (Blue Band, Golden Penny, La Prairie Classic) and animal fat containing samples (Kell Salad Cream, Crosse & Blackwell and Nola) was used for the purity check using the reversed phased - thin layer chromatographic (RPTLC) method of analysis were developed. The average Rf ratio of 0.95 and 0.92, found for the vegetable and animal fat groups were reported, respectively. The Rf = 0.03 difference between the two groups indicated the presence of sistosterol (plant sterol) and cholesterol (animal sterol), an improvement over color detection methods to screen oils and fats to ascertain purity. Keywords: Sistosterol, Cholesterol, Adulteration, Animal fat, Vegetable oil

Evaluation of reversed-phase thin layer chromatographic protocol to detect vegetable oil or fat in milk

2019 ◽  
Vol 72 (04) ◽  
pp. 370-374
Author(s):  
Sukthija . ◽  
Vivek Sharma . ◽  
Sumit Arora . ◽  
Richa Singh . ◽  
P N Raju . ◽  
...  
Keyword(s):  
Thin Layer ◽  
Vegetable Oil ◽  
Reversed Phase

Thin Layer and Gas-Liquid Chromatography of Cholesterol in Fats and Oils. I. Development of Method

1969 ◽  
Vol 52 (4) ◽  
pp. 774-778
Author(s):  
C W Thorpe ◽  
Linda Pohland ◽  
D Firestone
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Unsaponifiable Matter ◽  
Fats And Oils ◽  
Cholesterol Content ◽  
Gas Liquid Chromatography ◽  
Animal Fat ◽  
Low Levels ◽  
Layer Chromatography

Abstract A inethod is described for analysis of sterols by thin layer chromatography-gas liquid chromatography (TLC-GLC). Sterols are isolated from other components of unsaponifiable matter by preparative TLC. The sterols are quantitatively removed from the TLC plate, extracted from the silica gel, and analyzed by GLC. This method has been used to detect low levels (2–3%) of animal fat in vegetable oil by measuring the cholesterol content of the animal fatvegetable oil admixtures.

Influence of phospholipid saturation on classical thin-layer chromatographic detection methods and its effect on amniotic fluid lecithin/sphingomyelin ratio determinations.

1983 ◽  
Vol 29 (2) ◽  
pp. 250-255 ◽  
Author(s):  
T Spillman ◽  
D B Cotton ◽  
S C Lynn ◽  
J P Bretaudiere
Keyword(s):  
Thin Layer ◽  
Amniotic Fluid ◽  
Unsaturated Acid ◽  
Carbon Chain ◽  
Detection Methods ◽  
Detection Techniques ◽  
Color Development ◽  
Fatty Acid Carbon ◽  
Chromatographic Detection ◽  
Layer Chromatography

Abstract We compared seven techniques, commonly used for detection of amniotic fluid phospholipids in thin-layer chromatography, with respect to their sensitivity to saturation of the fatty acid carbon-chain of lecithin. The techniques fell into two classes: sensitive and insensitive; those classed as saturation sensitive were less than or equal to 10% as sensitive to fully saturated lecithin as to lecithin with singly unsaturated acid moieties. Color development increased with the number of carbon-carbon double bonds per molecule but required only a single unsaturated acid ester in either the alpha or the beta position. Mixtures of lecithins with defined saturation, when detected by saturation-sensitive methods, mimicked the uneven coloration of amniotic fluid lecithin bands, supporting the hypothesis that this uneven coloration results from natural saturation heterogeneity. Detection techniques representative of these classes are shown to give widely differing values for the lecithin/sphingomyelin ratio for actual specimens of amniotic fluid.

Reversed-phase thin-layer chromatography of some foodstuff dyes

2003 ◽  
Vol 16 (4) ◽  
pp. 276-279 ◽  
Author(s):  
Dušanka Milojković-Opsenica ◽  
Kristina Lazarević ◽  
Vojkan Ivačković ◽  
Živoslav Tešić
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Reversed Phase ◽  
Layer Chromatography

Chromo-ionophoric Properties of para-(5-phthalhydrazide-azo)-phenylene-N-aza-15-crown-5 in the Presence of Lithium and Sodium Ions

2008 ◽  
Vol 59 (10) ◽  
Author(s):  
Rodica Daniela Baratoiu ◽  
Radu Socoteanu ◽  
Radu Cristian Mutihac ◽  
Anca Elena Barbu ◽  
Adrian Beteringhe ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Reversed Phase ◽  
Complex Stability ◽  
Sodium Ions ◽  
Layer Chromatography

Investigations on the hydrophobic and ionophoric character of para-(5-phthalhydrazide-azo)-phenylene-N-aza-15-crown- (1) were carried out by reversed phase thin layer chromatography (RP-TLC) and spectrophotometric measurements. The study included complexes with Li+ and Na+ cations (as perchlorates in acetonitrile and as dibenzyldithiocarbamate salts in chloroform). In both cases, the stoichiometry of complexes was 1:1:1 (1:M+:anion pair). The complex stability (logKS ) is similar.

Sign in / Sign up

Export Citation Format

Share Document