Polymerase Chain Reaction (PCR) Detection Of The Genome Of African Swine Fever Virus (ASFV) From Natural Infection In A Nigerian Baby Warthog (<i>Phacochoereus Aethiopicus</i>)

African swine fever (ASF) suspected clinically in Madagascar (1998–9) was confirmed by polymerase chain reaction (PCR) and nucleotide sequencing, following virus isolation. No haemadsorption or cytopathic effect could be detected following leukocyte inoculation, but viral growth in cells was confirmed by PCR. Detection of ASF virus genome was carried out by amplification of a highly conserved region coding for the p72 protein. Nucleotide sequencing of the amplicon revealed 99·2% nucleotide identity between the recent Malagasy strains and a virus recovered from the 1994 outbreak in Mozambique (SPEC265). A serological survey performed on 449 sera, revealed that only 5·3% of the sera taken from pigs between 1998 and 1999 were positive.

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Polymerase Chain-Reaction (PCR) Detection of Rhinoviruses

Diagnosis of Human Viruses by Polymerase Chain Reaction Technology - Frontiers of Virology ◽

10.1007/978-3-642-84766-0_21 ◽

1992 ◽

pp. 272-284 ◽

Cited By ~ 1

Author(s):

Tim Skern ◽

Heinrich Kovar ◽

Gunhild Jug ◽

Herbert Auer ◽

Helge Torgensen ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Pcr Detection ◽

Chain Reaction ◽

Polymerase Chain

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DETECTION OF Leishmania (Viannia) IN Nyssomyia neivai AND Nyssomyia whitmani BY MULTIPLEX POLYMERASE CHAIN REACTION, IN SOUTHERN BRAZIL

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46652014000500004 ◽

2014 ◽

Vol 56 (5) ◽

pp. 391-395 ◽

Cited By ~ 9

Author(s):

Herintha Coeto Neitzke-Abreu ◽

Kárin Rosi Reinhold-Castro ◽

Mateus Sabaini Venazzi ◽

Regiane Bertin de Lima Scodro ◽

Alessandra de Cassia Dias ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Multiplex Pcr ◽

Multiplex Polymerase Chain Reaction ◽

Natural Infection ◽

Epidemiological Studies ◽

Leishmania Infection ◽

Southern Brazil ◽

Chain Reaction ◽

Polymerase Chain ◽

Sensitivity Specificity

Sandflies transmit pathogens of leishmaniasis. The natural infection of sandflies by Leishmania (Viannia) was assessed in municipalities, in the state of Paraná, in Southern Brazil. Sandflies were collected with Falcão and Shannon traps. After dissection in search of flagellates in digestive tubes and identification of the species, female sandflies were submitted to the Multiplex Polymerase Chain Reaction (multiplex PCR) for detection of the fragment of the kDNA of Leishmania (Viannia) and the fragment from the IVS6 cacophony gene region of the phlebotomine insects. The analysis was performed in pools containing seven to 12 guts from females of the same species. A total of 510 female sandflies were analyzed, including nine Migonemyia migonei, 17 Pintomyia fischeri, 216 Nyssomyia neivai, and 268 Nyssomyia whitmani. Although none of the females was found naturally infected by flagellates through dissection, the fragment of DNA from Leishmania (Viannia) was shown by multiplex PCR in one sample of Ny. neivai (0.46%) and three samples of Ny. whitmani (1.12%). It was concluded that Ny. neivai and Ny. whitmani are susceptible to Leishmania infection, and that multiplex PCR can be used in epidemiological studies to detect the natural infection of the sandfly vector, because of its sensitivity, specificity and feasibility.

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