scholarly journals The usefulness of microscopic observation for drug susceptibility of Mycobacterium tuberculosis complex in routine clinical microbiology laboratory

2009 ◽  
Vol 11 (2) ◽  
Author(s):  
SE Mshana ◽  
C Imirzalioglu ◽  
E Domann ◽  
T Chakraborty
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Microscopic Observation ◽  
Clinical Microbiology ◽  
Mycobacterium Tuberculosis Complex ◽  
Drug Susceptibility ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory ◽  
Tuberculosis Complex

scholarly journals Tuberculous Meningitis: The Microbiological Laboratory Diagnosis and Its Drug Sensitivity Patterns

2019 ◽  
Vol 3 (2) ◽  
pp. 35
Author(s):  
Titiek Sulistyowati ◽  
Deby Kusumaningrum ◽  
Eko Budi Koendhori ◽  
Ni Made Mertaniasih
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Tuberculous Meningitis ◽  
Drug Sensitivity ◽  
Mycobacterium Tuberculosis Complex ◽  
Laboratory Diagnosis ◽  
Clinical Microbiology Laboratory ◽  
Tuberculosis Complex ◽  
First Line ◽  
Sensitivity Pattern ◽  
Microbiological Laboratory

Background: Tuberculosis continues one of the major challenges to global health. Mycobacterium tuberculosis complex can affect any organ other than the lung parenchyma, include central nervous system. The mortality rate of tuberculous meningitis (TBM) are high worldwide with up to half of survivors suffering irreversible sequelae. Diagnosis of TBM is difficult due to paucibacillary, various clinical manifestation, and invasive procedure to appropriate specimens. Objective: The objectiveis to study the positivity rate of microbiological laboratory diagnosis and its drug sensitivity patterns of TBM patients in Dr. Soetomo Hospital Surabaya during October 2015 until September 2016. Methods: Specimens were cerebrospinal fluids. Identification and drug anti TB sensitivity test were done by BACTEC MGIT 960 system in Clinical Microbiology Laboratory Dr. Soetomo Hospital Surabaya. Result: Most patients with TBM were women (54.29%). Based on age groups, most dominant was adult population (65.71%). Proportion percentage of positive M. tuberculosis complex among 180 specimens were 19.44%. First line anti TB drug sensitivity pattern of 35 isolates were 1 monoresistant, 1 poly-resistant, no multiple drug resistant (MDR), and 33 pan-susceptible. Conclusion: Positivity rate of Mycobacterium tuberculosis complex laboratory diagnosis from TBM suspect patients were low. There was no MDR TB in this study, but mono-resistant and poly-resistant. Microbiological diagnosis was important to give information of active disease and drug sensitivity pattern. Resistance to first line anti TB drugs is alarming to properly manage TBM patients.

scholarly journals Trends in Testing for Mycobacterium tuberculosis Complex From US Public Health Laboratories, 2009–2013

2016 ◽  
Vol 132 (1) ◽  
pp. 56-64 ◽  
Author(s):  
Frances Tyrrell ◽  
Cortney Stafford ◽  
Mitchell Yakrus ◽  
Monica Youngblood ◽  
Andrew Hill ◽  
...  
Keyword(s):  
Public Health ◽  
Mycobacterium Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
The United States ◽  
Drug Susceptibility ◽  
Turnaround Time ◽  
Nucleic Acid Amplification ◽  
Tuberculosis Complex ◽  
Complex Detection ◽  
Susceptibility Tests

Objective: We investigated data from US public health laboratories funded through the Centers for Disease Control and Prevention’s Tuberculosis Elimination and Laboratory Cooperative Agreement to document trends and challenges in meeting national objectives in tuberculosis (TB) laboratory diagnoses. Methods: We examined data on workload and turnaround time from public health laboratories’ progress reports during 2009-2013. We reviewed methodologies, laboratory roles, and progress toward rapid detection of Mycobacterium tuberculosis complex through nucleic acid amplification (NAA) testing. We compared selected data with TB surveillance reports to estimate public health laboratories’ contribution to national diagnostic services. Results: During the study period, culture and drug susceptibility tests decreased, but NAA testing increased. Public health laboratories achieved turnaround time benchmarks for drug susceptibility tests at lower levels than for acid-fast bacilli smear and identification from culture. NAA positivity in laboratories among surveillance-reported culture-positive TB cases increased from 26.6% (2355 of 8876) in 2009 to 40.0% (2948 of 7358) in 2013. Public health laboratories provided an estimated 50.9% (4285 of 8413 in 2010) to 57.2% (4210 of 7358 in 2013) of culture testing and 88.3% (6822 of 7727 in 2011) to 94.4% (6845 of 7250 in 2012) of drug susceptibility tests for all US TB cases. Conclusions: Public health laboratories contribute substantially to TB diagnoses in the United States. Although testing volumes mostly decreased, the increase in NAA testing indicates continued progress in rapid M tuberculosis complex detection.

scholarly journals Susceptibilities of Mycobacterium tuberculosis Complex Isolates to First-line Antituberculosis Drugs at Dokuz Eylül University Hospital

2021 ◽  
Author(s):  
Nazlı Arslan ◽  
Müge Hacer Özkarataş ◽  
Nuran Esen ◽  
Aydan Özkütük
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
University Hospital ◽  
Surveillance Program ◽  
Tuberculosis Complex ◽  
First Line ◽  
Antituberculosis Drugs ◽  
Number Of Patients

Objective: Tuberculosis retains its importance as the only infectious disease in the world that affects 10 million people and causes 1.5 million deaths per se. The major obstacle in the elimination and control of tuberculosis is the emergence and spread of resistant tuberculosis cases. It was aimed to determine the current Mycobacterium tuberculosis complex and its susceptibility to antituberculosis drugs at Dokuz Eylül University Hospital. Method: In our study, the results of all samples sent between January 2013 and November 2019 were examined retrospectively for the presence of M. tuberculosis complex and drug susceptibility results. The samples were cultured in Löwenstein Jensen media and BACTEC MGIT 960 system. Drug susceptibility testing was performed with the BACTEC MGIT 960 SIRE kit in accordance with the recommendations of the manufacturer. Results: In a total of 473 (2.2%) of 21620 specimens M. tuberculosis complex was reproduced. The samples were classified as pulmonary (n:300; 63.4%) and extrapulmonary (n:173; 36.6%), samples. When repeated samples of the same patient, were excluded, positive culture test results were determined in a total of 365 patients. Susceptibility to all primary antituberculosis drugs was shown in 275 of 321 (85.7%) patients, while total rates of resistance to streptomycin, isoniazid, rifampicin and ethambutol were found in respective number of patients as follows: (n:24 (7.5%), 22 (6.8%), (n:7; 2.2%) and (n:2; 0.6%). The rate of MDR was 0.6% in 2 patients. Conclusion: In our hospital, streptomycin is the first-line antituberculosis drug with the highest resistance rate. All susceptibility rates were seen lower than the data reported in Turkey Tuberculosis Control Report and other studies of Turkey. Implementing drug surveillance program plays an important role for maintaining these low rates and for the management of tuberculosis.

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