Glucoamylase Production by a Newly Isolated Strain of Aspergillus niger

1982 ◽  
Vol 45 (7) ◽  
pp. 586-589 ◽  
Author(s):  
VILAS P. SINKAR ◽  
NORMAN F. LEWIS
Keyword(s):  
Aspergillus Niger ◽  
Submerged Culture ◽  
Crude Enzyme ◽  
Glucoamylase Production

A newly isolated indigenous fungus, Aspergillus niger #57 was found to be a prolific producer of glucoamylase. Stationary cultivation led to significantly higher yields than those obtained using submerged culture. The crude enzyme exhibited temperature and pH optima of 60°C and 4.0, respectively.

Diauxic production of glucose oxidase by Aspergillus niger in submerged culture

2002 ◽  
Vol 31 (5) ◽  
pp. 615-620 ◽  
Author(s):  
J. Mirón ◽  
M.P. González ◽  
L. Pastrana ◽  
M.A. Murado
Keyword(s):  
Aspergillus Niger ◽  
Glucose Oxidase ◽  
Submerged Culture

Induction and Repression Kinetics of Fungal Beta-Fructofuranosidase in Submerged Culture

2009 ◽  
Vol 5 (2) ◽  
Author(s):  
Armando Robledo-Olivo ◽  
Juan C Contreras-Esquivel ◽  
Raul Rodriguez Herrera ◽  
Cristobal N Aguilar
Keyword(s):  
Aspergillus Niger ◽  
Enzymatic Activity ◽  
Biomass Production ◽  
Submerged Culture ◽  
Enzyme Production ◽  
Maximum Rate ◽  
Sucrose Concentration ◽  
Viable Option ◽  
Initial Concentration ◽  
Kinetics Of

Invertase production by Aspergillus niger in submerged culture was evaluated under different concentrations of sucrose and glucose. When the initial concentration of sucrose was increased from 6.25 to 50 gL-1, a higher biomass production (6.1 gL-1) was observed. The biomass production was increased 4 times more when a glucose-sucrose combination was used as substrate (26.31 gL-1). The strain A. niger produced extracellular beta-fructofuranosidase activity at all test concentrations of the substrate and the highest enzymatic activity (3873 UL-1) was obtained when sucrose was used at 12.5 gL-1. However, with a glucose-sucrose concentration of 25 gL-1 the beta-fructofuranosidase activity was of 23706 UL-1. The maximum rate of invertase enzyme production in presence of sucrose by Aspergillus niger in submerged culture was 3.67 UL-1h-1 at 12.5 gL-1 concentration, while in the case of glucose-sucrose mixture, it was 13.95 UL-1h-1 at a concentration of 25 gL-1. It was observed that the enzyme yield (YE/X) was 1.25 times more in presence of sucrose than with glucose-sucrose combination. In addition, the results suggested that an addition of lower concentration of glucose is a viable option to increase the enzyme secretion by the fungi.

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