Comparison of Media for Isolation of Bacillus cereus from Foods

1985 ◽  
Vol 48 (11) ◽  
pp. 969-970 ◽  
Author(s):  
MATS PETERZ ◽  
CHRISTER WIBERG ◽  
PER NORBERG
Keyword(s):  
Bacillus Cereus ◽  
Egg Yolk ◽  
Food Products ◽  
Bromothymol Blue ◽  
Blood Agar ◽  
The Media ◽  
Dry Food ◽  
Better Than ◽  
Quantitative Recovery

Three media for isolation of Bacillus cereus from foods were compared: mannitol-egg yolk-polymyxin (MYP) agar, polymyxin pyruvate-egg yolk-mannitol-bromothymol blue agar (PEMBA) and non-selective blood agar. Twenty-six of 45 samples of different reconstituted and incubated dry food products and 18 of 29 samples of milk and cream (incubated overnight) contained B. cereus. None of the media performed significantly better than the others as regards quantitative recovery or selectivity.

OCCURRENCE OF BACILLUS CEREUS IN SELECTED DRY FOOD PRODUCTS1,2

1971 ◽  
Vol 34 (1) ◽  
pp. 12-15 ◽  
Author(s):  
H. U. Kim ◽  
J. M. Goepfert
Keyword(s):  
Bacillus Cereus ◽  
Egg Yolk ◽  
The United States ◽  
Food Samples ◽  
Confirmatory Test ◽  
Foodborne Disease ◽  
Biochemical Tests ◽  
Precipitin Test ◽  
Dry Food

One hundred seventy samples of dried food products in national distribution were examined for the incidence and level of contamination by Bacillus cereus. Twenty-five per cent of the samples yielded B. cereus at a level not exceeding 4000 per gram. Mannitol-egg yolk-polymyxin (MYP) agar was used as a presumptive test for the presence of B. cereus in the food samples. Various biochemical tests for the confirmation of suspicious colonies appearing on MYP agar were evaluated. A precipitin test employing spore precipitinogens was investigated as a confirmatory test for B. cereus. The possible role of B. cereus in outbreaks of foodborne disease in the United States is discussed.

scholarly journals DETECTION OF HBLD TOXIN GENE BY BACILLUS CEREUS ISOLATED FROM MEAT CURRY FOOD SAMPLES IN MALAYSIAN RESTAURANTS

2020 ◽  
Vol 4 (2) ◽  
pp. 68-72
Author(s):  
Marwan Msarah ◽  
Ahmed Alsier ◽  
Sahilah, A.M.
Keyword(s):  
Bacillus Cereus ◽  
Foodborne Pathogen ◽  
Food Poisoning ◽  
Egg Yolk ◽  
Food Samples ◽  
Blood Agar ◽  
Toxin Gene ◽  
Biochemical Tests ◽  
Isolation Medium ◽  
Hemolysin Bl

Bacillus cereus is a ubiquitous foodborne pathogen, can cause food poisoning, leading to infections, have two major types of food poisoning emetic and diarrheal. Foods rich in protein such as meat are associated with foodborne outbreaks of diarrhea caused by B. cereus. The aim of this study is to isolate and identify B. cereus from ready to eat (RTE) meat curry from restaurants in Malaysia and to detect hblD pathogenic gene of B. cereus isolates. Mannitol egg yolk polymyxin agar was used as a selective isolation medium. Commercially available kits and boiling methods were used for DNA extraction, samples acquired from restaurants were examined for the presence of Hemolysin BL gene by polymerase chain reaction (PCR). Among all isolates, twenty-four of B. cereus isolates detected for HBL enterotoxin production by the discontinuous pattern on HBL sheep blood agar then confirmed by biochemical tests. More than 58.33 % of the isolate showed discontinuous hemolysis pattern on HBl blood agar and 29.16% of the samples were shown positive for hblD gene that can cause diarrhea with the size of 807bp on gel. This study demonstrated that RTE meat curry was a potential source for entero-toxigenic B. cereus and the presence of the hblD toxin genes for the HBL complex in the isolates tested were highly associated. Therefore, these meat curry isolates should be regarded as potential toxin producers.

ISOLATION OF STAPHYLOCOCCI FROM DRIED MILK1

1965 ◽  
Vol 28 (4) ◽  
pp. 117-120 ◽  
Author(s):  
R. T. Marshall ◽  
C. D. Neighbors ◽  
J. E. Edmondson
Keyword(s):  
Salt Concentration ◽  
Egg Yolk ◽  
Food Products ◽  
Good Recovery ◽  
Coagulase Negative Staphylococci ◽  
Dry Milk ◽  
The Media ◽  
Quantitative Analyses ◽  
Coagulase Positive Staphylococci

Summary This study was made to evaluate, for use with dry milk, several media used in selective quantitative analyses for staphylococci in food products. The principal problems involved in culturing staphylococci from dry milk are (a) growth of Bacillus organisms and (b) obtaining good recovery of coagulase-positive staphylococci while eliminating growth of micrococci and coagulase-negative staphylococci, or at least providing a means of differentiating colonies of these organisms on the plates. Tellurite-polymyxin-egg yolk (TPEY) agar produced highest counts of coagulase-positive staphylococci for two-thirds of 40 strains tested in comparisons with Staphylococcus 110 (S110) and tellurite-glycine (TG) media. Highest counts of coagulase-negative staphylococci and of micrococci were found on S110 medium for 9 to 12 cultures. TG agar gave the poorest recovery in both experiments. TG and Vogel-Johnson (VJ) media were found too inhibitory to be used for staphylococcus counts on dry milk. Mannitol salt (MS) agar reacted very similarly to S110 agar. Colonies of Bacillus were easily discernible on TPEY medium but often were mistaken for staphylococci on the media which depend upon salt concentration for selectivity.

Improvement of Polymyxin–Egg Yolk–Mannitol–Bromothymol Blue Agar for the Enumeration and Isolation of Bacillus cereus in Various Foods

2017 ◽  
Vol 80 (3) ◽  
pp. 502-505
Author(s):  
Il-Byeong Kang ◽  
Jung-Whan Chon ◽  
Dong-Hyeon Kim ◽  
Dana Jeong ◽  
Hong-Seok Kim ◽  
...  
Keyword(s):  
Synergistic Effect ◽  
Bacillus Cereus ◽  
Egg Yolk ◽  
Bromothymol Blue ◽  
Food Samples ◽  
Red Pepper ◽  
Soybean Paste ◽  
Contaminated Food ◽  
Pepper Powder ◽  
Red Pepper Powder

ABSTRACT A modified polymyxin–egg yolk–mannitol–bromothymol blue agar (mPEMBA) was developed by supplementing polymyxin–egg yolk–mannitol–bromothymol blue agar (PEMBA) with trimethoprim to improve the selectivity for and recoverability of Bacillus cereus from naturally and artificially contaminated food samples. The number of B. cereus in mPEMBA was significantly higher than in PEMBA, indicating better recoverability (P < 0.05) in red pepper powder (PEMBA 0.80 ± 0.22 log CFU/g versus mPEMBA 1.95 ± 0.17 log CFU/g) and soybean paste (PEMBA 2.19 ± 0.18 log CFU/g versus mPEMBA 3.09 ± 0.13 log CFU/g). In addition, mPEMBA provided better visual differentiation of B. cereus colonies than PEMBA, which is attributable to the reduced number of competing microflora. We conclude that the addition of trimethoprim to PEMBA could generate a synergistic effect to improve selectivity for B. cereus.

An improved selective and diagnostic medium for the isolation and enumeration of Bacillus cereus in foods

1980 ◽  
Vol 26 (7) ◽  
pp. 753-759 ◽  
Author(s):  
R. Holbrook ◽  
Judith M. Anderson
Keyword(s):  
Bacillus Cereus ◽  
Egg Yolk ◽  
Bromothymol Blue ◽  
Food Samples ◽  
Selective Medium ◽  
Confirmatory Test ◽  
Staining Procedure ◽  
Complete Agreement ◽  
Phenol Red ◽  
Biochemical Tests

The use and performance of an improved diagnostic and selective medium, PEMBA (polymyxin pyruvate egg yolk mannitol bromothymol blue agar), for the detection of Bacillus cereus in foods is described. The distinct colonial appearance of B. cereus on PEMBA permitted the recognition of both strains: those that do precipitate egg yolk and those that do not react with egg yolk. A staining procedure, used to demonstrate microscopically both the presence of lipid globules in vegetative cells and spore morphology of isolates, proved a rapid and reliable confirmatory test which gave complete agreement with a battery of biochemical tests used for this purpose. The quantitative recovery of B. cereus on PEMBA from 143 food samples was not significantly different from counts on KG (Kim and Goepfert), MYP (mannitol egg yolk phenol red), and McClung's media, and the selectivity of PEMBA was generally superior.

Efficient Isolation and Identification of Bacillus cereus Group

2012 ◽  
Vol 95 (2) ◽  
pp. 446-451 ◽  
Author(s):  
Sandra M Tallent ◽  
Kristin M Kotewicz ◽  
Errol A Strain ◽  
Reginald W Bennett
Keyword(s):  
Bacillus Cereus ◽  
Dairy Products ◽  
Egg Yolk ◽  
Bromothymol Blue ◽  
Foodborne Illnesses ◽  
Isolation And Identification ◽  
Bacillus Cereus Group ◽  
Gram Positive ◽  
Contaminated Food ◽  
Chromogenic Agar

Abstract Bacillus cereus is a group of ubiquitous facultative anaerobic sporeforming Gram-positive rods commonly found in soil. The spores frequently contaminate a variety of foods, including produce, meat, eggs, and dairy products. Foodborne illnesses associated with toxins produced by B. cereus can result in self-limiting diarrhea or vomiting. Plate enumeration methods recommended by recognized food authorities to detect the presence of B. cereus in potentially contaminated food products do not inhibit other Gram-positive competitive bacteria. This study evaluated the use of Bacara, a new chromogenic agar, as an efficient method to identify and enumerate B. cereus group from food matrixes, even in the presence of background flora. Inclusivity and exclusivity testing was performed using four different selective and differential media for B. cereus, including Mannitol Egg Yolk Polymyxin (MYP), Polymyxin Pyruvate Egg-Yolk Mannitol Bromothymol Blue Agar, Bacillus Chromogenic Media, Brilliance, and Bacara. MYP and Bacara were also used in plate enumeration studies to isolate B. cereus from artificially contaminated foods.

PENGEMBANGAN MEDIA BIG BOOK UNTUK MENINGKATKAN HASIL BELAJAR PECAHAN SENILAI SISWA SD

2018 ◽  
Vol 16 (1) ◽  
pp. 67-76
Author(s):  
Disyacitta Neolia Firdana ◽  
Trimurtini Trimurtini
Keyword(s):  
Learning Outcomes ◽  
Large Scale ◽  
Fourth Grade ◽  
Learning Activities ◽  
Equivalent Fractions ◽  
Fourth Grade Students ◽  
Teacher Needs ◽  
The Media ◽  
Test Result ◽  
Better Than

This research aimed to determine the properness and effectiveness of the big book media on learning equivalent fractions of fourth grade students. The method of research is Research and Development  (R&D). This study was conducted in fourth grade of SDN Karanganyar 02 Kota Semarang. Data sources from media validation, material validation, learning outcomes, and teacher and students responses on developed media. Pre-experimental research design with one group pretest-posttest design. Big book developed consist of equivalent fractions material, students learning activities sheets with rectangle and circle shape pictures, and questions about equivalent fractions. Big book was developed based on students and teacher needs. This big book fulfill the media validity of 3,75 with very good criteria and scored 3 by material experts with good criteria. In large-scale trial, the result of students posttest have learning outcomes completness 82,14%. The result of N-gain calculation with result 0,55 indicates the criterion “medium”. The t-test result 9,6320 > 2,0484 which means the average of posttest outcomes is better than the average of pretest outcomes. Based on that data, this study has produced big book media which proper and effective as a media of learning equivalent fractions of fourth grade elementary school.

Selecting packaging material for dry food products by trade‐off of sustainability and performance: A case study on cookies and milk powder

2021 ◽  
Vol 34 (5) ◽  
pp. 303-318
Author(s):  
Maarten Baele ◽  
An Vermeulen ◽  
Dimitri Adons ◽  
Roos Peeters ◽  
Angelique Vandemoortele ◽  
...  
Keyword(s):  
Milk Powder ◽  
Food Products ◽  
Packaging Material ◽  
Trade Off ◽  
Dry Food ◽  
And Performance

Evaluation of iron, copper and zinc concentrations in commercial foods formulated for healthy cats

2021 ◽  
pp. 1098612X2110262
Author(s):  
Stacie Summers ◽  
Jonathan Stockman ◽  
Jennifer A Larsen
Keyword(s):  
Food Products ◽  
Metabolizable Energy ◽  
Life Stages ◽  
Trace Mineral ◽  
Iron Copper ◽  
Copper And Zinc ◽  
Zinc Concentrations ◽  
Dry Food ◽  
Raw Foods ◽  
Mineral Concentrations

Objectives The aim of this study was to analyze iron, copper and zinc concentrations in commercial foods and compare among food formats (dry, canned, raw), foods marketed by age category (adult 1+ years and senior 7+ years) and foods intended for adult maintenance vs all life stages. Methods In total, 112 commercial non-therapeutic food products marketed for healthy adult and senior cats were purchased in the USA. Foods were analyzed for their proximate composition. Trace mineral concentrations were measured using inductively coupled argon plasma–optical emission spectroscopy and described on a calculated metabolizable energy basis using standard modified Atwater values. Results Measured iron (median 58.4 mg/1000 kcal [range 15.7–379.0]), copper (median 5.6 mg/1000 kcal [range 0.8–13.3]) and zinc (median 47.6 mg/1000 kcal [range 7.6–128.1]) concentrations were highly variable among cat foods. When all food products – regardless of their nutritional adequacy substantiation method – were compared with the Association of American Feed Control Officials regulatory minimums, 13/112 food products had a mineral deficiency, of which a majority (n = 11/13) were raw food products. Raw foods had significantly lower trace mineral concentrations compared with dry food products and, except for copper, canned food products. Cat foods marketed for senior cats had higher iron ( P = 0.019) and zinc ( P <0.0001) concentrations than foods marketed for adult cats. Foods intended for adult maintenance had higher iron ( P = 0.003) and zinc concentrations than foods intended for all life stages ( P <0.0001). Conclusions and relevance Iron, copper and zinc concentrations in commercial non-therapeutic foods for adult and senior cats are highly variable. A minority of foods – mainly raw food products – were deficient in these minerals. It is unknown if some foods with high trace mineral concentrations could have adverse effects as studies are needed to establish safe upper limits for dietary intake of trace minerals in healthy cats.

The Social Construction of Corporate Violence: Media Coverage of the Imperial Food Products Fire

1995 ◽  
Vol 41 (1) ◽  
pp. 20-36 ◽  
Author(s):  
John P. Wright ◽  
Francis T. Cullen ◽  
Michael B. Blankenship
Keyword(s):  
Media Coverage ◽  
White Collar Crime ◽  
Food Products ◽  
White Collar ◽  
Newspaper Coverage ◽  
Processing Plant ◽  
Criminal Convictions ◽  
Safety Regulations ◽  
The Social ◽  
The Media

Although investigative reports have contributed to the social movement against white-collar crime, few studies assess the extent to which the media socially construct corporate violence as a “crime.” We examine this issue through a content analysis of newspaper coverage of the fire-related deaths of 25 workers at the Imperial Food Products chicken-processing plant, which resulted in the company's owner pleading guilty to manslaughter. The analysis revealed that newspaper reports largely attributed the deaths to the lax enforcement of safety regulations but did not initially construct the deaths as a crime or subsequently publicize the criminal convictions.

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