Evaluation of a Reversed Passive Latex Agglutination Test Kit for Clostridium perfringens Enterotoxin

1986 ◽  
Vol 49 (7) ◽  
pp. 523-525 ◽  
Author(s):  
STANLEY M. HARMON ◽  
DONALD A. KAUTTER
Keyword(s):  
Clostridium Perfringens ◽  
Immunosorbent Assay ◽  
Culture Supernatant ◽  
Food Poisoning ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Special Test ◽  
Clostridium Perfringens Enterotoxin ◽  
Test Kit

A reversed passive latex agglutination (RPLA) test kit for Clostridium perfringens enterotoxin (CPE) marketed by the Denka-Seiken Co., Tokyo, Japan, was evaluated by using culture supernatant fluids and extracts from feces of food poisoning patients. Nanograms of CPE were detectable with the assay and the reaction was specific, as shown by parallel activity in a double antibody enzyme-linked immunosorbent assay (ELISA). Although less sensitive, the RPLA method is easier to perform than the ELISA and counterimmunoelectrophoresis, both of which require special test reagents and equipment not generally available.

Prevalence of the Enterotoxin Gene and Clonality of Clostridium perfringens Strains Associated with Food-Poisoning Outbreaks

1998 ◽  
Vol 61 (2) ◽  
pp. 240-243 ◽  
Author(s):  
J. RIDELL ◽  
J. BJÖRKROTH ◽  
H. EISGRŰBER ◽  
B. SCHALCH ◽  
A. STOLLE ◽  
...  
Keyword(s):  
Clostridium Perfringens ◽  
Food Poisoning ◽  
Latex Agglutination ◽  
Pulsed Field Gel Electrophoresis ◽  
Enterotoxin Gene ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Clostridium Perfringens Enterotoxin ◽  
Polymerase Chain

The prevalence of the enterotoxin gene in a well-characterized collection of 71 Clostridium perfringens strains from 36 separate food-poisoning cases or outbreaks was analyzed with the polymerase chain reaction (PCR). The clonality of 39 strains originating from 14 outbreaks where at least two isolates were available was studied with pulsed-field gel electrophoresis (PFGE) using SmaI and ApaI restriction endonucleases. The cpe gene PCR assay was found to correlate well with Clostridium perfringens enterotoxin (CPE) production in vitro with reverse passive latex agglutination. Of the C. perfringens food and clinical food-poisoning isolates 24 (86%) and 38 (88%) were cpe-positive, respectively. Different PFGE patterns indicated that multiple cpe-positive clones are frequently present within one outbreak. The existence of cpe-positive and negative isolates with identical or nearly identical PFGE patterns in a single outbreak suggests that the cpe gene may be in a movable genetic element.

Seroepidemiology of Toxoplasma gondii in pet dogs and cats in Beijing, China

2008 ◽  
Vol 53 (3) ◽  
Author(s):  
Jinhai Yu ◽  
Jun Ding ◽  
Zhaofei Xia ◽  
Degui Lin ◽  
Yili Li ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Age Groups ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Pet Dogs ◽  
Significant Difference ◽  
Beijing China

AbstractSera from 534 pet dogs and 335 pet cats from Beijing (China) were tested for anti-Toxoplasma gondii antibodies using an enzyme-linked immunosorbent assay or the latex agglutination test. The seropositivity by year, season, sex and age was analysed. Overall, 128 dogs (24.0%) and 50 cats (14.9%) had antibodies to T. gondii. When analysed by season, the highest seroprevalence was found in spring for dogs (31.3%) and cats (25.1%), and the differences in seroprevalence by season was statistically significant in cats (P<0.01) but not in dogs. The seroprevalence in male dogs (23.7%) and cats (15.1%) were slightly higher than their female counterparts (18.0% in dogs and 12.3% in cats). There was no obvious pattern of seropositivity or significant difference in different age groups in dogs or cats; nonetheless, a high proportion of dogs at 4 years of age were positive to T. gondii (31.8%) while cats with relatively high seropositivity rates were at 1 or 3.4 years of age (13.14%).

scholarly journals Serological diagnosis of Toxoplasmosis by specific antibody in camels of Wasit province, Iraq

2018 ◽  
Vol 1 (29) ◽  
pp. 412-423
Author(s):  
Basim Mohammed Hanon
Keyword(s):  
Public Health ◽  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Specific Antibody ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Serological Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Blood Samples ◽  
Zoonotic Parasite

Background: toxoplasma gondii is a zoonotic parasite, more added a major public health is worldwide because have high distribution in livestock. Objectives: the main aim of this study determine the occurrence of the seroepidemiological toxoplasmosis in camels in waist province of Iraq from November 2016 to April 2017. Materials and Methods: blood samples collected of animals randomly were included six different groups of animals were diagnosed by A Latex agglutination test (LAT) and indirect enzyme linked immunosorbent assay (ELISA) kits.

scholarly journals An investigation of sheep toxoplasmosis in Basrah Province / Iraq

2010 ◽  
Vol 9 (3) ◽  
pp. 128
Author(s):  
K. M. Al-Saad ◽  
Saad Hashim Al-Husseiny
Keyword(s):  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Indirect Elisa ◽  
Elisa Test ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Study Results ◽  
Significant Difference ◽  
The Difference

The objective of this study was to investigate Toxoplasma gondii antibodies among sheep in different regions of Basrah province (including Al-Mdayna, Shatt Al-Arab, Al-Basrah, Al-Zubayr, and Abu Al-Khasib). The study was started in Oct. 2008 and was finished in May 2009, using latex agglutination test (LAT) and indirect enzyme linked immunosorbent assay (ELISA) IgG, 309 adult sheep were randomly selected from 15 herds among different ages and both sexes and used in this study, including 62 pregnant ewes, 185 non-pregnant ewes, 14 aborted ewes, and 48 rams. Results showed, that 60.84% were seropositive by LAT, whereas 51.11% were seropositive by ELISA IgG test, among animals used in this study, results detected that 79.03% pregnant ewes (highest value), 56.75% nonpregnant ewes,71.40% aborted ewes and 50% Rams (lowest value) were seropositive by LAT, whereas 56.52% pregnant ewes, 51.11% non-pregnant ewes, 83.33% aborted ewes (highest value), and 31.25% Rams (lowest value) were seropositive by indirect ELISA IgG. Moreover, among regions of Basrah province, the details of percentage of T.gondii antibodies were 54.54% in AL-Basrah , 71.43% in Abu Al-Khasib (highest value), 57.35% in Al- Mdayna, 47.83% in Shatt Al-Arab (lowest value), and 67.16% in Al-Zubayr by LAT, whereas 63.64% in AL-Basrah (highest value), 22.73% in Abu Al-Khasib (lowest value), 57.89% in Al-Mdayna, 50% in Shatt Al-Arab and 61.90% in Al-Zubayr by indirect ELISA test. Although the difference observed in the percentage of Toxoplasma gondii antibodies among different regions of Basrah, there was no significant difference P>0.05 detected LAT, whereas in the indirect ELISA IgG test there was significant difference P<0.05. Ewes showed high percentages 62.83%, 55.40% of toxoplasmosis than rams 50 %, 31.25% by LAT and ELISA test respectively. The highest titer was 1/4 28.57% were detected in pregnant ewes and lowest titers were 1/2, 1/8, and 1/256 0.0% were detected in aborted ewes and in ramsrespectively.

Comparison of LFCI, LAT and ELISA for the diagnosis of toxoplasmosis in goats

2015 ◽  
Author(s):  
Sadaf Niaz ◽  
Rafi Ullah ◽  
Bahrawar Said ◽  
Sumaira Shams ◽  
Naser M. AbdEl-Salam ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Lateral Flow ◽  
Latex Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Blood Samples ◽  
Male And Female ◽  
Toxoplasma Gondii Infection

This study was carried out in order to investigate the seroprevalence of Toxoplasma gondii infection in goats of seven different regions of District Dir (Lower), Pakistan. Lateral Flow Chromatographic Immunoassay (LFCI), Latex Agglutination Test (LAT) and Enzyme-Linked Immunosorbent Assay (ELISA) were used for the detection of T. gondii infection in goats. A total of 175 blood samples were collected from goats. Out of which 70 (40%) were found positive for T. gondii. The prevalence of toxoplasmosis among goats in seven areas of District Dir (Lower) was found high at Samarbagh (48%), Balambat (44%) followed by khall (40%), Munda (40%), LalQilla (36%) and lowest in Adenzai and Timergara (36%). This study demonstrated that the prevalence in older goats was significantly (P< 0.05) higher (48.91%) than younger ones (30.12%). The seroprevalence in male and female goats were 16 (29%) and 54 (45%) respectively.

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