Disinfectant and Antibiotic Susceptibility Profiles of Escherichia coli O157:H7 Strains from Cattle Carcasses, Feces, and Hides and Ground Beef from the United States†

2013 ◽  
Vol 76 (1) ◽  
pp. 6-17 ◽  
Author(s):  
ROSS C. BEIER ◽  
TONI L. POOLE ◽  
DAYNA M. BRICHTA-HARHAY ◽  
ROBIN C. ANDERSON ◽  
KENNETH M. BISCHOFF ◽  
...  
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Antibiotic Susceptibility ◽  
Ground Beef ◽  
The United States ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Inducible Resistance ◽  
Susceptibility Profiles

The disinfectant and antibiotic susceptibility profiles of 344 Escherichia coli O157:H7 strains from cattle carcasses, feces, and hides and ground beef from the United States were determined. A low prevalence of antibiotic resistance was observed (14%). The highest prevalences of resistance were to sulfisoxazole (10.5%), tetracycline (9.9%), streptomycin (7%), and chloramphenicol (4.9%). Four strains were resistant to eight antibiotics (two strains from ground beef and one strain each from hide and preevisceration carcass swabs of cull cattle at harvest). Pulsed-field gel electrophoresis analysis of the E. coli O157:H7 strains revealed two major groups (designated 1 and 2) composed of 17 and 20 clusters, respectively. Clusters 1A, 1B, 1C, and 1G.1 were associated with multidrug-resistant strains. There was no observed correlation between disinfectant resistance and antibiotic resistance. Sixty-nine (20%) of the 344 strains were resistant to chlorhexidine or benzalkonium chloride or the MICs of benzyldimethyldodecylammonium chloride were elevated. Inducible resistance was observed at elevated concentrations of antibiotics (1.4%) and disinfectants (6.1%). The highest rate of disinfectant inducible resistance was to OdoBan, quaternary ammonium chlorides, and the surface disinfectants F25, FS512, and MG, which are used in dairies, restaurants, and food processing plants. High MICs (1,024 to 4,096 μg/ml) of acetic, lactic, and citric acids were found. The decreasing order of acid potency based on molar MICs (MICsmolar) was acetic, citric, and lactic acid. The correlation of the concentration of dissociated organic acids and MICsmolar strongly suggests that the observed inhibition of E. coli O157:H7 was primarily due to dissociated forms of the acids.

scholarly journals Comparison of the Reveal 20-Hour Method and the BAM Culture Method for the Detection of Escherichia coli O157:H7 in Selected Foods and Environmental Swabs: Collaborative Study

2001 ◽  
Vol 84 (3) ◽  
pp. 737-751 ◽  
Author(s):  
Charles B Bird ◽  
Rebecca J Hoerner ◽  
Lawrence Restaino ◽  
G Anderson ◽  
W Birbari ◽  
...  
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
Collaborative Study ◽  
Culture Method ◽  
The United States ◽  
Escherichia Coli O157 ◽  
Private Industry ◽  
E Coli ◽  
Different Levels

Abstract Four different food types along with environmental swabs were analyzed by the Reveal for E. coli O157:H7 test (Reveal) and the Bacteriological Analytical Manual (BAM) culture method for the presence of Escherichia coli O157:H7. Twenty-seven laboratories representing academia and private industry in the United States and Canada participated. Sample types were inoculated with E. coli O157:H7 at 2 different levels. Of the 1095 samples and controls analyzed and confirmed, 459 were positive and 557 were negative by both methods. No statistical differences (p <0.05) were observed between the Reveal and BAM methods.

Fecal Shedding of Escherichia coli O157:H7 in North Dakota Feedlot Cattle in the Fall and Spring

2006 ◽  
Vol 69 (5) ◽  
pp. 1154-1158 ◽  
Author(s):  
MARGARET L. KHAITSA ◽  
MARC L. BAUER ◽  
GREGORY P. LARDY ◽  
DAWN K. DOETKOTT ◽  
REDEMPTA B. KEGODE ◽  
...  
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
North Dakota ◽  
The United States ◽  
Extreme Weather ◽  
Early Spring ◽  
Feedlot Cattle ◽  
Escherichia Coli O157 ◽  
Fecal Shedding ◽  
E Coli

Cattle are an important reservoir of Escherichia coli O157:H7, which can lead to contamination of food and water, and subsequent human disease. E. coli O157:H7 shedding in cattle has been reported as seasonal, with more animals shedding during summer and early fall than during winter. North Dakota has relatively cold weather, especially in winter and early spring, compared with many other regions of the United States. The objective was to assess fecal shedding of E. coli O157: H7 in North Dakota feedlot cattle over the fall, winter, and early spring. One hundred forty-four steers were assigned randomly to 24 pens on arrival at the feedlot. Samples of rectal feces were obtained from each steer four times (October and November 2003, and March and April 2004) during finishing. On arrival (October 2003), 2 (1.4%) of 144 cattle were shedding E. coli O157:H7. The shedding increased significantly to 10 (6.9%) of 144 after 28 days (November 2003), to 76 (53%) of 143 at the third sampling (March 2004), and dropped significantly to 30 (21%) of 143 at the fourth (last) sampling (March 2004) before slaughter. Unfortunately, we were unable to sample the cattle during winter because of the extreme weather conditions. Sampling time significantly (P < 0.0001) influenced variability in E. coli O157:H7 shedding, whereas herd (P = 0.08) did not. The prevalence of E. coli O157:H7 shedding in North Dakota steers in fall and early spring was comparable to what has been reported in other parts of the United States with relatively warmer weather. Further research into E. coli O157:H7 shedding patterns during extreme weather such as North Dakota winters is warranted in order to fully assess the seasonal effect on the risk level of this organism.

How Does Escherichia coli O157:H7 Testing in Meat Compare with What We Are Seeing Clinically?

2000 ◽  
Vol 63 (6) ◽  
pp. 819-821 ◽  
Author(s):  
DAVID W. K. ACHESON
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
Shiga Toxin ◽  
Food Supply ◽  
The United States ◽  
Escherichia Coli O157 ◽  
Current Policy ◽  
E Coli ◽  
Different Types ◽  
Uremic Syndrome

Escherichia coli O157:H7 is but one of a group of Shiga toxin-producing E. coli (STEC) that cause both intestinal disease such as bloody and nonbloody diarrhea and serious complications like hemolytic uremic syndrome (HUS). While E. coli O157: H7 is the most renowned STEC, over 200 different types of STEC have been documented in meat and animals, at least 60 of which have been linked with human disease. A number of studies have suggested that non-O157 STEC are associated with clinical disease, and non-O157 STEC are present in the food supply. Non-O157 STEC, such as O111 have caused large outbreaks and HUS in the United States and other countries. The current policy in the United States is to examine ground beef for O157:H7 only, but restricting the focus to O157 will miss other important human STEC pathogens.

Epidemiology of Escherichia coli O157 in Feedlot Cattle

1997 ◽  
Vol 60 (5) ◽  
pp. 462-465 ◽  
Author(s):  
DALE D. HANCOCK ◽  
DANIEL H. RICE ◽  
LEE ANN THOMAS ◽  
DAVID A. DARGATZ ◽  
THOMAS E. BESSER
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
The United States ◽  
Feedlot Cattle ◽  
Escherichia Coli O157 ◽  
Fecal Samples ◽  
E Coli ◽  
Geographical Regions ◽  
Flagellar Antigen ◽  
Low Prevalence

Fecal samples from cattle in 100 feedlots in 13 states were bacteriologically cultured for Escherichia coli O157 that did not ferment sorbitol, lacked beta-glucuronidase, and possessed genes coding for Shiga-like toxin. In each feedlot 30 fresh fecal-pat samples were collected from each of four pens: with the cattle shortest on feed, with cattle longest on feed, and with cattle in two randomly selected pens. E. coli O157 was isolated from 210 (1.8%) of 11,881 fecal samples. One or more samples were positive for E. coli O157 in 63 of the 100 feedlots tested. E. coli O157 was found at roughly equal prevalence in all the geographical regions sampled. The prevalence of E. coli O157 in the pens with cattle shortest on feed was approximately threefold higher than for randomly selected and longest on feed pens. Of the E. coli O157 isolates found in this study, 89.52% expressed the H7 flagellar antigen. E. coli O157 was found to be widely distributed among feedlot cattle, but at a low prevalence, in the United States.

The Aerobic Plate Count, Coliform and Escherichia coli Content of Raw Ground Beef at the Retail Level

1976 ◽  
Vol 39 (3) ◽  
pp. 175-178 ◽  
Author(s):  
J. M. GOEPFERT
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
New York ◽  
Ground Beef ◽  
The United States ◽  
Plate Count ◽  
Raw Meat ◽  
E Coli ◽  
Step Procedure ◽  
Aerobic Plate Count

Nine hundred fifty-five samples of raw ground beef obtained from supermarkets throughout the United States were examined for coliforms, Escherichia coli, and Aerobic Plate Count (APC). The results were compared with existent standards for E. coli in raw meat in New York and Oregon. Lack of homogenious distribution of E. coli in fresh ground beef was demonstrated. Observations were made that indicate that a 2 day-two step procedure will detect the same number of E. coli as the more time consuming four step MPN procedure 98% of the time. A comparison of the APC obtained by incubating plates at 20 C and 35 C showed there to be an average 10-fold difference with the 20 C incubation always higher. Questions are raised about the necessity of microbial standards for raw meat and the validity of incorporating E. coli in such standards.

Transmission and control of Escherichia coli O157:H7 — A review

2002 ◽  
Vol 82 (4) ◽  
pp. 475-490 ◽  
Author(s):  
S. J. Bach ◽  
T. A. McAllister ◽  
D. M. Veira ◽  
V. P. J. Gannon ◽  
R. A. Holley
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
Foodborne Pathogen ◽  
The United States ◽  
Urban Environments ◽  
Effective Control ◽  
Feed Water ◽  
Escherichia Coli O157 ◽  
Bacteriophage Therapy ◽  
E Coli

Escherichia coli 157:H7 has evolved as an important foodborne pathogen since its initial description in 1982. Outbreaks of illness associated with E. coli O157:H7 have been reported throughout the northern hemisphere, most frequently in Canada, the United States, Japan, and the United Kingdom. In Canada, infections due to E. coli O157: H7 appear to be more common in the western provinces than in the east, in rural vs. urban environments, and during summer as opposed to winter months. Undercooked ground beef has been implicated as the primary vehicle in E. coli O157:H7 infection, but contaminated fruits, vegetables and water have also been linked to E. coli O157:H7 outbreaks. Epidemiological investigations demonstrate that dairy and beef cattle are primary reservoirs of this organism, carrying it asymptomatically and shedding it intermittently and seasonally in their feces. Surveys in Canada and the United States indicate widespread distribution of E. coli O157:H7 in cattle operations. The prevalence of E. coli O157:H7 in cattle has been increasing in recent reports, likely due to the development of more sensitive methods for the detection of the organism. Escherichia coli O157:H7 has been isolated from feed, water for livestock, manure, soil and flies, all of which represent potential sources of contamination for cattle and their environment. To date, effective methods for controlling E. coli O157:H7 in cattle have not been identified, although dietary manipulation, vaccination and bacteriophage therapy have been reported to have potential as intervention strategies. Effective control of E. coli O157:H7 requires reducing the frequency and intensity of fecal shedding of this pathogen by cattle, in addition to targeting environmental sources of the organism. Key words: Escherichia coli O157:H7, cattle, sources, diet, transmission

Prevalence and Enumeration of Escherichia coli O157:H7 and Salmonella in U.S. Abattoirs that Process Fewer than 1,000 Head of Cattle per Day†

2009 ◽  
Vol 72 (6) ◽  
pp. 1272-1278 ◽  
Author(s):  
JOSEPH M. BOSILEVAC ◽  
TERRANCE M. ARTHUR ◽  
JAMES L. BONO ◽  
DAYNA M. BRICHTA-HARHAY ◽  
NORASAK KALCHAYANAND ◽  
...  
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
The United States ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Beef Processing ◽  
Processing Plants

A significant portion (15 to 20%) of beef in the United States is produced in small beef processing plants that harvest fewer than 1,000 cattle per day. However, there are little data on the prevalence and levels of Escherichia coli O157:H7 and Salmonella in these processing plants. To address this lack of data, hides (n = 1,995) and carcasses (n = 1,995) of cattle at seven small processing plants located across the United States were analyzed for E. coli O157:H7 and Salmonella. Across all plants, hide prevalence of E. coli O157:H7 and Salmonella was 71 and 91%, respectively. Twelve percent of hides had E. coli O157:H7 at enumerable levels (≥40 CFU/100 cm2), while 36% of hides had Salmonella at enumerable levels. Across all plants, the prevalence of E. coli O157:H7 on preevisceration carcasses was 33%, with 2% at an enumerable level (≥0.8 CFU/100 cm2). Across all plants, Salmonella prevalence on preevisceration carcasses was 58%, with 8% at an enumerable level. Significant plant-to-plant variations in levels and prevalence of pathogens on carcasses were detected. Reduced levels of pathogens on carcasses were noted among small processors that had incorporated a hide-directed intervention. The results obtained are comparable to those observed previously for larger processors, showing that smaller beef processors face and address the same challenges as do larger beef processors.

scholarly journals Prevalence of Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella in Two Geographically Distant Commercial Beef Processing Plants in the United States†

2004 ◽  
Vol 67 (2) ◽  
pp. 295-302 ◽  
Author(s):  
MILDRED RIVERA-BETANCOURT ◽  
STEVEN D. SHACKELFORD ◽  
TERRANCE M. ARTHUR ◽  
KURT E. WESTMORELAND ◽  
GINA BELLINGER ◽  
...  
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
The United States ◽  
Conveyor Belt ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Beef Processing ◽  
Processing Plants ◽  
Northern United States

For two large beef processing plants, one located in the southern United States (plant A) and one located in the northern United States (plant B), prevalence of Escherichia coli O157:H7, Listeria spp., Listeria monocytogenes, and Salmonella was determined for hide, carcass, and facility environmental samples over the course of 5 months. The prevalence of E. coli O157: H7 (68.1 versus 55.9%) and Salmonella (91.8 versus 50.3%) was higher (P < 0.05), and the prevalence of Listeria spp. (37.7 versus 75.5%) and L. monocytogenes (0.8 versus 18.7%) was lower (P < 0.05) for the hides of cattle slaughtered at plant A versus plant B. Similarly, the prevalence of Salmonella (52.0 versus 25.3%) was higher (P < 0.05) and the prevalence of Listeria spp. (12.0 versus 40.0%) and L. monocytogenes (1.3 versus 14.7%) was lower (P < 0.05) for the fence panels of the holding pens of plant A versus plant B. The prevalence of E. coli O157:H7 (3.1 versus 10.9%), Listeria spp. (4.5 versus 14.6%), and L. monocytogenes (0.0 versus 1.1%) was lower (P < 0.05) for preevisceration carcasses sampled at plant A versus plant B. Salmonella (both plants), Listeria spp. (plant B), and L. monocytogenes (plant B) were detected on fabrication floor conveyor belts (product contact surfaces) late during the production day. For plant B, 21 of 148 (14.2%) late-operational fabrication floor conveyor belt samples were L. monocytogenes positive. For plant B, E. coli O157:H7 and L. monocytogenes were detected in preoperational fabrication floor conveyor belt samples. Overall results suggest that there are regional differences in the prevalence of pathogens on the hides of cattle presented for harvest at commercial beef processing plants. While hide data may reflect the regional prevalence, the carcass data is indicative of differences in harvest practices and procedures in these plants.

Survival of Listeria monocytogenes and Escherichia coli O157:H7 during Sauerkraut Fermentation

2005 ◽  
Vol 68 (7) ◽  
pp. 1367-1374 ◽  
Author(s):  
MIOMIR NIKSIC ◽  
STEVEN E. NIEBUHR ◽  
JAMES S. DICKSON ◽  
AUBREY F. MENDONCA ◽  
JEFFREY J. KOZICZKOWSKI ◽  
...  
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Titratable Acidity ◽  
The United States ◽  
Escherichia Coli O157 ◽  
Total Acidity ◽  
E Coli ◽  
Acid Tolerant ◽  
Traditional Process

Sauerkraut was produced from shredded cabbage, as is typical in the United States, and from whole head cabbages, which is a traditional process in parts of Eastern Europe. The sauerkraut was inoculated with five strain mixtures of Escherichia coli O157:H7 and Listeria monocytogenes, and the populations of these bacteria, as well as lactic acid bacteria, pH, and titratable acidity, were monitored over the course of fermentation. Fermentation variables were temperature (18 and 22°C) and salt concentration (1.8, 2.25, and 3%). For most of the analyses, the type of cabbage processing was a significant factor, although within cabbage type, neither salt nor fermentation temperature had significant effects. The final pH of the whole-head sauerkraut was lower than the shredded sauerkraut, but the titratable acidity was significantly higher in the shredded sauerkraut. E. coli O157:H7 and L. monocytogenes persisted in the brines for most of the fermentation, although at the end of the fermentations (15 days for shredded, 28 days for whole head), neither pathogen had detectable populations. E. coli populations decreased more rapidly in the shredded sauerkraut even though the pH was higher because of the higher total acidity in the shredded sauerkraut. Acid-tolerant strains of E. coli and L. monocytogenes were isolated from both shredded and whole-head sauerkraut at different salt concentrations and temperatures after 15 days of fermentation and could be detected at 35 days in the whole-head sauerkraut.

scholarly journals Prevalence of Escherichia coli O157:H7 in Organically and Naturally Raised Beef Cattle

2009 ◽  
Vol 75 (16) ◽  
pp. 5421-5423 ◽  
Author(s):  
S. Reinstein ◽  
J. T. Fox ◽  
X. Shi ◽  
M. J. Alam ◽  
D. G. Renter ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Beef Cattle ◽  
Antibiotic Susceptibility ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Antibiotic Susceptibility Patterns ◽  
Susceptibility Profiles ◽  
Susceptibility Patterns

ABSTRACT We determined the prevalence of Escherichia coli O157:H7 in organically and naturally raised beef cattle at slaughter and compared antibiotic susceptibility profiles of the isolates to those of isolates from conventionally raised beef cattle. The prevalences of E. coli O157:H7 were 14.8 and 14.2% for organically and naturally raised cattle, respectively. No major difference in antibiotic susceptibility patterns among the isolates was observed.

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