Serological and molecular detection of Theileria equi in horses from Sinop, Mato Grosso state, Brazil

ABSTRACT: The increasing expansion of visceral leishmaniasis (VL) in the Brazilian territory evidences the need for studies focused on the main reservoir of this parasite: the dog. This study aimed to conduct an epidemiological survey in the municipality of Barão de Melgaço, Pantanal region of the state of Mato Grosso (MT), Brazil. Conventional polymerase chain reaction (PCR) and qualitative SYBR®Green real-time PCR (qPCR) were used to diagnose canine VL (CVL) and characterize the factors associated with this infection. Of the 402 dogs that had blood samples collected, 31 presented the parasite DNA, representing a prevalence of 7.71% in the population studied. Positivity indices for PCR and qPCR were 3.48 (14/402) and 7.21% (29/402), respectively. Comparison of the results obtained by both techniques showed moderate agreement (Kappa = 0.5364). Of the independent variables analyzed, presence of clinical signs (p≤0.05) was the only one associated with CVL. Based on this study, we conclude that VL is a circulating disease, with relatively low prevalence, in dogs of Barão de Melgaço/MT, and that the presence of clinical signs is the only variable associated with canine infection.

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Molecular detection of Rickettsia spp., Anaplasma platys and Theileria equi in ticks collected from horses in Tayrona National Park, Colombia

Experimental and Applied Acarology ◽

10.1007/s10493-019-00354-8 ◽

2019 ◽

Vol 77 (3) ◽

pp. 411-423

Author(s):

Adriana Santodomingo ◽

Keyla Sierra-Orozco ◽

Andrea Cotes-Perdomo ◽

Lyda R. Castro

Keyword(s):

National Park ◽

Molecular Detection ◽

Anaplasma Platys ◽

Theileria Equi

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Molecular detection of feline arthropod-borne pathogens in cats in Cuiabá, state of Mato Grosso, central-western region of Brazil

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612013000300011 ◽

2013 ◽

Vol 22 (3) ◽

pp. 385-390 ◽

Cited By ~ 25

Author(s):

Natasha Gandolfi Miceli ◽

Fernando Antonio Gavioli ◽

Luiz Ricardo Gonçalves ◽

Marcos Rogério André ◽

Valéria Régia Franco Sousa ◽

...

Keyword(s):

Molecular Detection ◽

Western Region ◽

Mato Grosso ◽

Animal Shelters ◽

Blood Samples ◽

Cytauxzoon Felis ◽

The City ◽

Veterinary Hospital

Hemotrophic mycoplasmas (hemoplasmas), Bartonellasp., Hepatozoon sp. and Cytauxzoon felis are prominent pathogens that circulate between cats and invertebrate hosts. The present study aimed to detect the presence of DNA from hemoplasmas,Bartonella sp., Hepatozoon sp. andCytauxzoon felis, and then confirm it by means of sequencing, in blood samples from cats in Cuiabá, MT, Brazil. From February 2009 to February 2011, blood samples with added EDTA were collected from 163 cats that were being housed in four different animal shelters in the city of Cuiabá, state of Mato Grosso, Brazil and from 15 cats that were admitted to the veterinary hospital of the Federal University of Mato Grosso (UFMT). Out of the 178 cats sampled, 15 (8.4%) were positive for hemoplasmas: four (2.2%) forMycoplasma haemofelis, 12 (6.7%) for ‘Candidatus M. haemominutum’ and one (0.5%) for ‘Candidatus M. turicensis’. One cat (0.5%), a patient that was attended at the veterinary hospital, was coinfected with M. haemofelis, ‘Candidatus M. haemominutum’ and ‘Candidatus M. turicensis’, based on sequencing confirmation. Four cats were positive for Bartonella spp.: three (1.7%) for B. henselae and one (0.5%) for B. clarridgeiae. None of the animals showedCytauxzoon sp. or Hepatozoon sp. DNA in their blood samples. This study showed that cats housed in animal shelters in the city of Cuiabá, state of Mato Grosso, are exposed to hemoplasmas andBartonella species.

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Serological and molecular detection of Theileria equi infection in horses in Hungary

Veterinary Parasitology ◽

10.1016/j.vetpar.2012.09.035 ◽

2013 ◽

Vol 192 (1-3) ◽

pp. 143-148 ◽

Cited By ~ 18

Author(s):

R. Farkas ◽

B. Tánczos ◽

M. Gyurkovszky ◽

G. Földvári ◽

N. Solymosi ◽

...

Keyword(s):

Molecular Detection ◽

Theileria Equi

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Molecular detection of Hepatozoon canis and Babesia canis vogeli in domestic dogs from Cuiabá, Brazil

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612011000300015 ◽

2011 ◽

Vol 20 (3) ◽

pp. 253-255 ◽

Cited By ~ 16

Author(s):

Mariana Granziera Spolidorio ◽

Mariana de Medeiros Torres ◽

Wilma Neres da Silva Campos ◽

Andréia Lima Tomé Melo ◽

Michelle Igarashi ◽

...

Keyword(s):

18S Rrna ◽

Molecular Detection ◽

Domestic Dogs ◽

Hepatozoon Canis ◽

Babesia Canis ◽

Mato Grosso ◽

First Time ◽

The City ◽

Babesia Spp

The objective of this study was to report for the first time infection by Hepatozoon spp. and Babesia spp. in 10 dogs from the city of Cuiabá, State of Mato Grosso, central-western Brazil. A pair of primers that amplifies a 574 bp fragment of the 18S rRNA of Hepatozoon spp., and a pair of primers that amplifies a 551 bp fragment of the gene 18S rRNA for Babesia spp. were used. Six dogs were positive for Babesia spp., and 9 were positive for Hepatozoon spp. Co-infection of Babesia spp. and Hepatozoon spp. was seen in 5 dogs. Sequenced samples revealed 100% identity with B. canis vogeli, and H. canis. This is the first molecular detection of H. canis in domestic dogs from Cuiabá. Additionally, it is described for the first time the presence of B. canis vogeli circulating among dogs in Cuiabá.

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