This paper examines the level of difficulty lecturers experience in constructing test items for various types of tests based on gender and institutional mode of delivery in an Open and Distance Learning higher education context. Descriptive cross-sectional survey research was adopted as the research paradigm, using lecturers from one single mode and one dual mode ODL institution in South West Nigeria as the study population. Stratified simple random sampling techniques were used to select 240 lecturers as the sample. Three research questions and two hypotheses guided the study’s investigation. A questionnaire with a reliability coefficient of 0.78 using Cronbach’s alpha value was used as the research instrument. Frequency, percentages, t-test statistics and charts were utilised to analyse the data. The results showed that ODL lecturers exhibited moderate levels of difficulty in test item construction. Female ODL lecturers found case study, multiple choice, matching, essay, and completion items more difficult to generate than their male counterparts did. There was a significant difference in the difficulty male and female ODL lecturers experienced in constructing test items. Significant differences were also found in the difficulty experienced by single and dual mode ODL lecturers in construct test items. The findings of the study have implications for the capacity development of lecturers, ODL institution management, and test experts in order to improve lecturers’ capacities in test items construction.