Biodiversity assessment of African locust bean (Parkia biglobosa) accessions from Savanna and Forest zones of Nigeria as revealed by seed storage proteins and RAPD markers

Understanding the level and distribution of genetic diversity in African locust bean (Parkia biglobosa) would strengthen breeding and conservation programmes towards domestication and sustainable use of this species. Sixteen accessions of P. globosa were assessed for variability based on seed morphology, seed protein and DNA profiling. Significant variation in seed characteristics were observed across locations. Seed protein profiling by SDS-PAGE revealed homogeneity as most bands were found common in all accessions, indicating that the protein profiles are highly conserved. Protein profiling separated the 16 accessions into four major clusters at 0.93 similarity coefficient. Most accessions grouping into Cluster 1 had a similarity coefficient of close to 100% and were from the Derived Savanna suggesting the presence of duplicates. Accessions NH/2016/P14, NH/2016/P03 and NH/2016/P04 grouped into clusters II, III and IV; respectively. Sixteen RAPD markers generated a total of 256 bands of which 63.67% were polymorphic. Gene diversity ranged from 0.41 to 0.93 and Polymorphic Information Content (PIC) from 0.39 to 0.93. The RAPD-based dendrogram separated accessions into six groups at 0.68 similarity coefficient. Based on a polymorphic seed storage protein marker a genetically distinct accession NH/2016/P04 could be exploited for breeding purposes. The homogeneity of alleles and narrow genetic base as revealed by RAPD and SDS-PAGE analyses suggests possible loss of intraspecific genetic diversity. Thus, intensification of germplasm collections across the different agroecological zones and characterization using specific markers will give a better understanding of diversity of P. biglobosa in order to enhance selection towards conservation, breeding and sustainable utilization.

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Flow cytometric analysis of nuclear DNA content, mitotic chromosome number and protein separation by SDS-PAGE in three accessions of African locust bean (Parkia biglobosa Benth.)

Journal of Crop Science and Biotechnology ◽

10.1007/s12892-011-0043-1 ◽

2011 ◽

Vol 14 (4) ◽

pp. 227-232 ◽

Cited By ~ 4

Author(s):

Edak Aniedi Uyoh ◽

Ikootobong Sunday Urua ◽

Valentine Otang Ntui ◽

Elza Cletus Okpako

Keyword(s):

Protein Separation ◽

Mitotic Chromosome ◽

Nuclear Dna ◽

Flow Cytometric Analysis ◽

Nuclear Dna Content ◽

Parkia Biglobosa ◽

Flow Cytometric ◽

Sds Page ◽

Locust Bean ◽

African Locust Bean

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Assessment of genetic diversity among chickpea (Cicer arietinum L.) genotypes using seed storage protein profiling and rapd markers

Applied Biological Research ◽

10.5958/0974-4517.2017.00037.4 ◽

2017 ◽

Vol 19 (3) ◽

pp. 248

Author(s):

H.J. Maisuria ◽

R.M. Patel ◽

K.P. Suthar

Keyword(s):

Genetic Diversity ◽

Cicer Arietinum ◽

Storage Protein ◽

Rapd Markers ◽

Seed Storage Protein ◽

Seed Storage ◽

Protein Profiling ◽

Cicer Arietinum L

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Evaluating genetic biodiversity of Parkia biglobosa (Jacq.) R. Br. Ex Don (African locust bean) accessions from Nigeria using seed protein electrophoresis

Journal of Crop Science and Biotechnology ◽

10.1007/s12892-014-0119-9 ◽

2015 ◽

Vol 18 (3) ◽

pp. 171-180 ◽

Cited By ~ 1

Author(s):

Adenubi I. Adesoye ◽

Kayode A. Apo

Keyword(s):

Seed Protein ◽

Protein Electrophoresis ◽

Parkia Biglobosa ◽

Seed Protein Electrophoresis ◽

Genetic Biodiversity ◽

Locust Bean ◽

African Locust Bean

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Fermentation of African locust bean (Parkia biglobosa) seeds: involvement of different species of Bacillus

Food Microbiology ◽

10.1016/0740-0020(88)90018-4 ◽

1988 ◽

Vol 5 (4) ◽

pp. 195-199 ◽

Cited By ~ 17

Author(s):

L.J Ogbadu ◽

R.N Okagbue

Keyword(s):

Parkia Biglobosa ◽

Locust Bean ◽

African Locust Bean

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Technologies traditionnelles de transformation des graines de néré (Parkia biglobosa Jacq. R.Br.) en Afrique de l’Ouest : revue des principaux produits dérivés et contraintes de production.

Journal of Applied Biosciences ◽

10.35759/jabs.152.8 ◽

2020 ◽

Vol 152 ◽

pp. 15698-15708

Author(s):

COULIBALY DIAKITE Mariam ◽

PARKOUDA Charles ◽

COMPAORE Sidbewendé Clarisse ◽

SAVADOGO Aly

Keyword(s):

West Africa ◽

Burkina Faso ◽

Parkia Biglobosa ◽

Afrique De L’Ouest ◽

Time Data ◽

Research Perspectives ◽

Traditional Technologies ◽

Locust Bean ◽

African Locust Bean ◽

Afrique De L'ouest

Les graines de néré (Parkia biglobosa) sont transformées traditionnellement en Afrique de l’Ouest en des condiments fermentés sous différentes appellations : soumbala au Burkina Faso, nététu au Sénégal, soumbara en Guinée Conakry, dawadawa et iru au Nigéria, afitin, iru et sonru au Bénin. Ces produits sont caractérisés par la variabilité de leur qualité et des contraintes liées à leur production. La présente revue a pour objectif d’analyser les technologies traditionnelles utilisées pour la production de ces condiments fermentés à base de graines de néré et de faire ressortir les contraintes/difficultés de production de ces condiments. Les données ont été collectées à travers les documents physiques consultés (thèses et mémoires) et à partir des articles scientifiques et d’autres documents trouvés en ligne. Il en résulte que les technologies de production traditionnelle des condiments à base de graine de néré en Afrique de l’Ouest, incluent principalement selon les zones et les ethnies, une première cuisson des graines allant de 12-48 h, un décorticage manuel des graines cuites, une seconde cuisson des graines décortiquées et lavées variant de 1-4 h et enfin, une fermentation des cotylédons cuits allant de 24-120 h. Ces technologies ont également en commun, des contraintes majeures de pénibilités du processus de cuisson, du décorticage/lavage, de la consommation importante d’énergie, d’eau et de temps. Les données de cette revue ouvrent ainsi de nouvelles pistes de recherche pour l’amélioration des procédés traditionnels de transformation des graines de néré. Mots clés : Graines de néré, condiment, soumbala, procédé, pénibilité. Coulibaly Diakite et al., J. Appl. Biosci. 2020 Technologies traditionnelles de transformation des graines de néré (Parkia biglobosa Jacq. R.Br.) en Afrique de l’Ouest : revue des principaux produits dérivés et contraintes de production 15699 Traditional technologies for processing African locust bean seeds (Parkia biglobosa Jacq. R.Br.) in West Africa: review on the main derivatives and production constraints. ABSTRACT African locust bean (Parkia biglobosa) seeds are traditionally transformed in West Africa into fermented condiments under different names: soumbala in Burkina Faso, netetu in Senegal, soumbara in Guinea Conakry, dawadawa and iru in Nigeria, afitin, iru and sonru in Benin. These products are characterized by the variability of their quality and the constraints linked to their production. The objective of this study is to analyze the traditional technologies used for the production of these fermented condiments and to highlight the constraints/difficulties associated to their production. The data were collected through the physical documents consulted (theses and dissertations) and from scientific articles and other documents found online. It appear that the traditional technologies used to produce P. biglobosa seeds based-condiments in West Africa, mainly include, according to the zones and ethnic groups, a first cooking of the seeds ranging from 12-48 h, a manual dehulling of the cooked seeds, a second cooking of the dehulled and washed seeds varying from 1-4 h and finally, a fermentation of the cooked cotyledons ranging from 24-120 h. These technologies have in common the major constraints of hardness of the process of cooking and dehulling/washing, significant consumption of energy, water and time. Data from this review opens research perspectives for the improvement of the traditional processing of P. biglobosa seeds. Keywords: P. biglobosa seeds, condiment, soumbala, process.

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The intravarietal relationship among different accessions of Dolichos biflorus was checked based on protein profiling using SDS-PAGE.

American Journal of Biotechnology and Bioinformatics ◽

10.28933/ajobb-2020-12-2505 ◽

2021 ◽

pp. 9

Author(s):

Keyword(s):

Genetic Diversity ◽

Protein Profiling ◽

Similarity Matrix ◽

High Genetic Diversity ◽

Dolichos Biflorus ◽

Band Matrix ◽

Sds Page ◽

Upgma Cluster Analysis ◽

Clustering Pattern ◽

100 Seed Weight

In this study, the phylogenetic relationship within the selected Eleven Indian (Dolichos biflorus (horse gram) varieties was analyzed for total soluble seed protein. Twenty-five bands were documented through SDS PAGE based on 100 seed weight of each variety and were studied for genetic diversity. Jaccard’s similarity matrix was acquired and used in UPGMA cluster analysis based on the polymorphism generated by the presence (1) or absence (0) of protein bands. Thus, the dendrogram showed four major groups that corre-spond to an earlier study on polymorphisms of 11 accessions of Indian Dolichos. Signifi-cant correspondence between the clustering pattern and the pedigree was observed; thus, a high genetic diversity could be kept within the Dolichos varieties. A similarity matrix among the targeted genotypes and phylogenetic analysis is considered a unique feature in the present work. Therefore, the current investigation was carried out to analyse Protein diversity of unexplored Dolichos genotypes at the molecular level, construct a dendro-gram based on similarity band matrix and generate efficiency in genetic divergence analy-sis among Dolichos. This study underlines the importance of using genetic diversity based in the Dolichos breeding program.

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