scholarly journals A greenhouse screening technique for bean golden mosaic virus

1969 ◽  
Vol 80 (1-2) ◽  
pp. 65-72
Author(s):  
Cristóbal Adames-Mora ◽  
James S. Beaver ◽  
O. Díaz
Keyword(s):  
Mosaic Virus ◽  
Dry Bean ◽  
Bean Golden Mosaic Virus ◽  
Inoculation Method ◽  
Snap Beans ◽  
Stage Of Development ◽  
Bean Plants ◽  
Resistant Lines ◽  
The Caribbean ◽  
Southern Florida

Dry bean (Phaseolus vulgaris L.) yield in the Caribbean and snap bean production in southern Florida are threatened by bean golden mosaic virus (BGMV). Effective screening techniques are needed in order to transfer BGMV resistance into red kidney, red mottied and snap beans. A greenhouse inoculation method for BGMV using viruiiferous whiteflies (Bemisia tabaci Genn.) has been developed. The use of this inoculation method insures that bean plants are inoculated at the same stage of development with a uniform amount of inoculum. In the greenhouse, the reaction of bean plants to BGMV could be determined within 30 days after planting, whereas field evaluations require up to 65 days. In addition, the BGMV reaction of bean genotypes inoculated in different experiments was found to be repeatable. In susceptible genotypes, such as PC50, typical BGMV symptoms began to appear between five to eight days after inoculation (DAI). In the resistant line DOR364, symptoms did not begin to appear until 10 to 12 DAI and the severity of the symptoms was less than in susceptible genotypes. Pompadour G and lines derived from crosses with DOR303 showed a dwarfing response when inoculated with BGMV.The BGMV resistant lines derived from crosses with A429 did not develop symptoms when inoculated with the viruliferous whiteflies.This greenhouse inoculation technique may facilitate the effort to combine different forms of BGMV resistance.

scholarly journals Phenotypic and molecular screening of dry bean (Phaseolus vulgaris L.) breeding lines for resistance to bean common mosaic virus and bean common mosaic necrosis virus

2021 ◽  
Vol 20 (6) ◽  
pp. 7-18
Author(s):  
İlyas Deligoz ◽  
Miray Arlı-Sökmen ◽  
Mucella Tekeoglu
Keyword(s):  
Mosaic Virus ◽  
Agricultural Research ◽  
Bean Common Mosaic Virus ◽  
Virus Species ◽  
Necrosis Virus ◽  
Dry Bean ◽  
Breeding Lines ◽  
Bean Plants ◽  
Resistant Lines ◽  
Recessive Genes

Bean common mosaic virus (BCMV) and bean common mosaic necrosis virus (BCMNV) are among the most economically important virus species infecting common bean. The use of resistant plant cultivars is the most effective way to control these viruses. National dry bean breeding studies have been conducted by seven different governmental agricultural research institutes in Turkey, and advanced breeding lines have been developed by using the selected local dry bean populations and crossing studies. In this study, 204 breeding lines were tested for resistance levels to BCMV and BCMNV. Initially, BCMNV NL-3 and BCMV NL-4 strains were individually sap-inoculated onto the leaves of bean plants belonging to each breeding lines with 10 replications, and the reactions of plants were evaluated for symptomatic appearance of virus infection 21 days after inoculation. Additionally, phenotypic evaluation was confirmed by molecular markers linked to resistance genes. As a result of the study, 153 breeding lines were found to involve the dominant I gene whereas four and five of the tested lines had the recessive genes bc-1² and bc-2², respectively. In conclusion, it was emphasized that these breeding lines could be registered after evaluating them in terms of yield and quality. Also, the use of seeds of the resistant lines to supply the source of virus-resistance in breeding studies and maintaining their seeds at the national genebank were recommended.

scholarly journals A Codominant Randomly Amplified Polymorphic DNA (RAPD) Marker Useful for Indirect Selection of Bean Golden Mosaic Virus Resistance in Common Bean

1996 ◽  
Vol 121 (6) ◽  
pp. 1035-1039 ◽  
Author(s):  
Carlos A. Urrea ◽  
Phillip N. Miklas ◽  
James S. Beaver ◽  
Ronald H. Riley
Keyword(s):  
Common Bean ◽  
Mosaic Virus ◽  
Partial Resistance ◽  
Recombinant Inbred Lines ◽  
Rapd Marker ◽  
The Other ◽  
Dry Bean ◽  
Snap Bean ◽  
Codominant Marker ◽  
Bean Golden Mosaic Virus

Bean golden mosaic virus (BGMV) is a devastating disease of common bean (Phaseolus vulgaris L.) in tropical America. The disease is effectively controlled by combinations of genetic resistances. The most widely deployed source of resistance to BGMV is a recessive gene (bgm-1) derived from the dry bean landrace cultivar Garrapato (Mexico) that conditions a nonmosaic partial resistance response to the pathogen. To expedite introgression of partial resistance into snap bean for southern Florida and other susceptible dry bean market classes for the Caribbean and Central American regions, a RAPD marker tightly linked to bgm-1 has been identified. Two contrasting DNA bulks, one consisting of five BGMV-resistant and the other five susceptible F6 recombinant inbred lines, were used to screen for polymorphic fragments amplified by 300 decamer primers in the polymerase chain reaction. RAPDs generated between the bulks were analyzed across F2 populations segregating for the marker and the gene. One codominant RAPD marker (R2570/530) tightly linked to the recessive resistance gene bgm-1 was found. The 530-base pair (bp) fragment was linked in repulsion with bgm-1 and the other 570-bp fragment was linked in coupling. No recombinants between R2570/530 and bgm-1 were observed among 91 F2 progeny from one dry bean population, and there were two recombinants (4.2 cM) observed among 48 F2 progeny combined across four snap bean populations. Assays of R2570/530 across susceptible germplasm and lines likely to have the `Garrapato'-derived partial resistance to BGMV have revealed that the codominant marker is gene-pool nonspecific and maintains its original linkage orientation with the recessive bgm-1 gene through numerous meioses. The codominant marker is useful for rapidly introgressing partial resistance to BGMV into susceptible germplasm.

scholarly journals Reaction of Select Tepary Bean to Bean Golden Mosaic Virus

HortScience ◽  
1996 ◽  
Vol 31 (3) ◽  
pp. 430-432 ◽  
Author(s):  
Phillip N. Miklas ◽  
Jose Santiago
Keyword(s):  
Puerto Rico ◽  
Mosaic Virus ◽  
Seed Yield ◽  
Seed Quality ◽  
Tepary Bean ◽  
Phaseolus Acutifolius ◽  
Dry Bean ◽  
Bean Golden Mosaic Virus ◽  
Dry Seasons ◽  
The Tropics

Cultivated tepary bean (Phaseolus acutifolius A. Gray var. latifolius Freeman) has potential for production during the hot, dry seasons in the tropics. Bean golden mosaic virus (BGMV), however, seriously limits production of Phaseolus spp. in such environments. Twelve select tepary beans were evaluated for reaction to BGMV across four field nurseries near Isabela, Puerto Rico. Disease reaction was principally determined by measurement of seed yield (kg·ha–1) and weight (g 100/seeds). All tepary beans possessed some tolerance to BGMV, as they produced comparatively moderate seed yield despite expression of severe foliar yellow mosaic symptoms. On average, tepary bean yielded 133% of the BGMV-resistant dry bean (Phaseolus vulgaris L.) control `Dorado'. Four teparies, Neb-T-6-s, GN-610-s, Neb-T-8a-s, and PI 321637-s, expressed superior tolerance to BGMV as they yielded above the trial mean in at least three of four trials. Harvested seed quality was uniformly poor across all lines, averaging 18% less weight than in the non-BGMV trials. The combination of the observed tolerance with escape mechanisms and cultural disease control practices may enable production of tepary bean in regions and seasons that experience moderate to severe BGMV epidemics.

scholarly journals Maize resistance to Sugarcane mosaic virus

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 542-544
Author(s):  
R. Pokorný ◽  
M. Porubová
Keyword(s):  
Mosaic Virus ◽  
Systemic Infection ◽  
Sugarcane Mosaic Virus ◽  
Resistant Line ◽  
Mechanisms Of Resistance ◽  
Long Distance ◽  
Maize Hybrids ◽  
Resistant Lines ◽  
Maize Resistance ◽  
Long Distance Movement

Under greenhouse conditions 12 maize hybrids derived from crosses of four resistant lines with several lines of different level of susceptibility were evaluated for resistance to Czech isolate of Sugarcane mosaic virus (SCMV). These hybrids were not fully resistant to isolate of SCMV, but the symptoms on their newly growing leaves usually developed 1 to 3 weeks later in comparison with particular susceptible line, the course of infection was significantly slower and rate of infection lower. As for mechanisms of resistance, the presence of SCMV was detected by ELISA in inoculated leaves both of resistant and susceptible lines, but virus was detected 7 days later in resistant line. Systemic infection developed only in susceptible lines. These results indicate restriction of viral long distance movement in the resistant line.

Evidence for a divided genome in bean golden mosaic virus, a geminivirus

Nature ◽  
1981 ◽  
Vol 289 (5795) ◽  
pp. 324-326 ◽  
Author(s):  
S. Haber ◽  
M. Ikegami ◽  
N. B. Bajet ◽  
Robert M. Goodman
Keyword(s):  
Mosaic Virus ◽  
Bean Golden Mosaic Virus

scholarly journals Seleção de progênies de feijoeiro F4 resistentes ao Bean golden mosaic virus

2005 ◽  
Vol 30 (3) ◽  
pp. 279-285 ◽  
Author(s):  
Fernando C. Juliatti ◽  
Simone A. Moraes ◽  
Heyder D. Silva ◽  
Márcio H. C. Borges
Keyword(s):  
Phaseolus Vulgaris ◽  
Mosaic Virus ◽  
Bean Golden Mosaic Virus

O objetivo deste trabalho foi identificar progênies de feijoeiro (Phaseolus vulgaris) na geração F4 provenientes do cruzamento entre cultivares resistentes ao Bean golden mosaic virus (BGMV). Os parentais foram as cultivares Carioca-MG (suscetível) e IAPAR-57, IAPAR-72 e IAPAR-65 (resistentes). A população de 480 progênies foi obtida pelo modelo dialélico completo. Estas foram semeadas em campo sob inoculação natural e estudada ao nível de plantas individuais para cálculo do índice de doença de BGMV (I.D.) Em relação ao I.D. médio, as progênies foram mais resistentes do que seus parentais (2,62 e 2,87), respectivamente. O processo de seleção foi realizado pelo I.D. (sigmag² = 0,2729 e h a² = 0,3953), onde foram obtidas famílias com grãos do tipo carioca e com I.D. inferior aos parentais resistentes. Segundo o GS% estimado, não serão necessárias muitas progênies ou famílias para se obter progresso com seleção para BGMV. O genótipo IAPAR-72 foi o parental superior na obtenção de progênies de maior resistência (menores I.D.). Possivelmente o mecanismo de resistência é do tipo resistência parcial.

scholarly journals Detection of Bean golden mosaic virus in Fabaceae family plants / Detecção de Bean golde nmosaic vírus em plantas da família Fabaceae

2021 ◽  
Vol 4 (1) ◽  
pp. 1021-1032
Author(s):  
Aline Taiane de Freitas-Vanzo ◽  
Camila de Cassia da Silva ◽  
Vitória Carolina Antunes Chaves ◽  
Mateus Henrique Garcia ◽  
Luana Thomaz de Aquino ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Bean Golden Mosaic Virus
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