scholarly journals Phenotypic and molecular screening of dry bean (Phaseolus vulgaris L.) breeding lines for resistance to bean common mosaic virus and bean common mosaic necrosis virus

2021 ◽  
Vol 20 (6) ◽  
pp. 7-18
Author(s):  
İlyas Deligoz ◽  
Miray Arlı-Sökmen ◽  
Mucella Tekeoglu
Keyword(s):  
Mosaic Virus ◽  
Agricultural Research ◽  
Bean Common Mosaic Virus ◽  
Virus Species ◽  
Necrosis Virus ◽  
Dry Bean ◽  
Breeding Lines ◽  
Bean Plants ◽  
Resistant Lines ◽  
Recessive Genes

Bean common mosaic virus (BCMV) and bean common mosaic necrosis virus (BCMNV) are among the most economically important virus species infecting common bean. The use of resistant plant cultivars is the most effective way to control these viruses. National dry bean breeding studies have been conducted by seven different governmental agricultural research institutes in Turkey, and advanced breeding lines have been developed by using the selected local dry bean populations and crossing studies. In this study, 204 breeding lines were tested for resistance levels to BCMV and BCMNV. Initially, BCMNV NL-3 and BCMV NL-4 strains were individually sap-inoculated onto the leaves of bean plants belonging to each breeding lines with 10 replications, and the reactions of plants were evaluated for symptomatic appearance of virus infection 21 days after inoculation. Additionally, phenotypic evaluation was confirmed by molecular markers linked to resistance genes. As a result of the study, 153 breeding lines were found to involve the dominant I gene whereas four and five of the tested lines had the recessive genes bc-1² and bc-2², respectively. In conclusion, it was emphasized that these breeding lines could be registered after evaluating them in terms of yield and quality. Also, the use of seeds of the resistant lines to supply the source of virus-resistance in breeding studies and maintaining their seeds at the national genebank were recommended.

NL-3 K Strain Is a Stable and Naturally Occurring Interspecific Recombinant Derived from Bean common mosaic necrosis virus and Bean common mosaic virus

2005 ◽  
Vol 95 (9) ◽  
pp. 1037-1042 ◽  
Author(s):  
Richard C. Larsen ◽  
Phillip N. Miklas ◽  
Keri L. Druffel ◽  
Stephen D. Wyatt
Keyword(s):  
Amino Acids ◽  
Mosaic Virus ◽  
Molecular Size ◽  
Bean Common Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Necrosis Virus ◽  
Bean Plants ◽  
Recombination Point ◽  
Russian Strain ◽  
Polymerase Chain

A strain of Bean common mosaic necrosis virus (BCMNV) from Idaho was identified by enzyme-linked immunosorbent assay using monoclonal antibodies and determined to be similar to the NL-3 D strain (of Drifjhout) by reaction of differential bean cultivars. However, this BCMNV strain (designated NL-3 K) caused earlier and more severe symptoms on bean plants representing host groups 0, 4, and 5. The nucleotide sequence encoding the predicted polyprotein of NL-3 K was 9,893 nucleotides (nt) in length, yielding a peptide with a molecular size of 362.1 kDa compared with a 9,626-nt, 350.9-kDa polyprotein for NL-3 D. Sequence analysis of the putative P1 protein suggests that the NL-3 K strain is a recombinant between NL-3 D and the Russian strain (RU1) of Bean common mosaic virus. The P1 protein of NL-3 K consisted of 415 amino acids compared with 317 for NL-3 D. The first 114 predicted amino acids of the NL-3 K P1 region were 98% identical with RU1. The remaining 301 amino acids of the protein shared only 34% identity with RU1 but were 98% identical with NL-3 D. Primers were designed that flanked the recombination point in the P1 coding sequence of NL-3 K. An amplicon of the expected size was produced by reverse-transcriptase polymerase chain reaction of total nucleic acid extracts of bean plants inoculated with NL-3 K, but not from those with NL-3 D or RU1. The increased symptom severity on selected common bean lines induced by NL-3 K suggests that the P1 gene may play a significant role in pathogenicity and virulence.

scholarly journals A greenhouse screening technique for bean golden mosaic virus

1969 ◽  
Vol 80 (1-2) ◽  
pp. 65-72
Author(s):  
Cristóbal Adames-Mora ◽  
James S. Beaver ◽  
O. Díaz
Keyword(s):  
Mosaic Virus ◽  
Dry Bean ◽  
Bean Golden Mosaic Virus ◽  
Inoculation Method ◽  
Snap Beans ◽  
Stage Of Development ◽  
Bean Plants ◽  
Resistant Lines ◽  
The Caribbean ◽  
Southern Florida

Dry bean (Phaseolus vulgaris L.) yield in the Caribbean and snap bean production in southern Florida are threatened by bean golden mosaic virus (BGMV). Effective screening techniques are needed in order to transfer BGMV resistance into red kidney, red mottied and snap beans. A greenhouse inoculation method for BGMV using viruiiferous whiteflies (Bemisia tabaci Genn.) has been developed. The use of this inoculation method insures that bean plants are inoculated at the same stage of development with a uniform amount of inoculum. In the greenhouse, the reaction of bean plants to BGMV could be determined within 30 days after planting, whereas field evaluations require up to 65 days. In addition, the BGMV reaction of bean genotypes inoculated in different experiments was found to be repeatable. In susceptible genotypes, such as PC50, typical BGMV symptoms began to appear between five to eight days after inoculation (DAI). In the resistant line DOR364, symptoms did not begin to appear until 10 to 12 DAI and the severity of the symptoms was less than in susceptible genotypes. Pompadour G and lines derived from crosses with DOR303 showed a dwarfing response when inoculated with BGMV.The BGMV resistant lines derived from crosses with A429 did not develop symptoms when inoculated with the viruliferous whiteflies.This greenhouse inoculation technique may facilitate the effort to combine different forms of BGMV resistance.

Bean Common Mosaic Virus and Bean Common Mosaic Necrosis Virus

2015 ◽  
pp. 1-46 ◽  
Author(s):  
Elizabeth A. Worrall ◽  
Francis O. Wamonje ◽  
Gerardine Mukeshimana ◽  
Jagger J.W. Harvey ◽  
John P. Carr ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Bean Common Mosaic Virus ◽  
Necrosis Virus

Distribution and localization of bean common mosaic virus and bean black root virus in stems of doubly infected bean plants

1994 ◽  
Vol 138 (1-2) ◽  
pp. 95-104 ◽  
Author(s):  
J. A. Khan ◽  
H. Lohuis ◽  
R. W. Goldbach ◽  
J. Dijkstra
Keyword(s):  
Mosaic Virus ◽  
Bean Common Mosaic Virus ◽  
Bean Plants

scholarly journals The I Gene of Bean: A Dosage-Dependent Allele Conferring Extreme Resistance, Hypersensitive Resistance, or Spreading Vascular Necrosis in Response to the Potyvirus Bean common mosaic virus

2000 ◽  
Vol 13 (11) ◽  
pp. 1266-1270 ◽  
Author(s):  
Candace Whitmer Collmer ◽  
Marcia Fisher Marston ◽  
Jessica C. Taylor ◽  
Molly Jahn
Keyword(s):  
Mosaic Virus ◽  
Bean Common Mosaic Virus ◽  
Extreme Resistance ◽  
Hypersensitive Resistance ◽  
Plant Host ◽  
Viral Pathogen ◽  
Bean Plants ◽  
Allele Dosage ◽  
Phloem Necrosis ◽  
I Gene

The resistance to the potyvirus Bean common mosaic virus (BCMV) conferred by the I allele in cultivars of Phaseolus vulgaris has been characterized as dominant, and it has been associated with both immunity and a systemic vascular necrosis in infected bean plants under field, as well as controlled, conditions. In our attempts to understand more fully the nature of the interaction between bean with the I resistance allele and the pathogen BCMV, we carefully varied both I allele dosage and temperature and observed the resulting, varying resistance responses. We report here that the I allele in the bean cultivars we studied is not dominant, but rather incompletely dominant, and that the system can be manipulated to show in plants a continuum of response to BCMV that ranges from immunity or extreme resistance, to hypersensitive resistance, to systemic phloem necrosis (and subsequent plant death). We propose that the particular phenotypic outcome in bean results from a quantitative interaction between viral pathogen and plant host that can be altered to favor one or the other by manipulating I allele dosage, temperature, viral pathogen, or plant cultivar.

scholarly journals Bean common mosaic virus and Bean common mosaic necrosis virus in Mexico

Plant Disease ◽  
2003 ◽  
Vol 87 (1) ◽  
pp. 21-25 ◽  
Author(s):  
N. Flores-Estévez ◽  
J. A. Acosta-Gallegos ◽  
L. Silva-Rosales
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Bean Common Mosaic Virus ◽  
Nucleotide Sequences ◽  
Necrosis Virus ◽  
Cp Gene

A survey was performed in Mexico to study the distribution of Bean common mosaic virus (BCMV) and Bean common mosaic necrosis virus (BCMNV) using a set of primers directed to the coat protein gene (CP) that were designed to detect and characterize the two viral species. Both viral species were present in different locations in the country. BCMV was predominant in the central states of the country, whereas BCMNV proliferated toward the eastern tropical states. The alignment of nine nucleotide sequences for each viral species at the amino region of the CP gene confirmed the identities of the viruses and set the basis to assign them tentatively to pathogroups I, II, and VI.

scholarly journals First Report of Bean common mosaic necrotic virus Infecting Bean Plants in Aguascalientes and Veracruz, Mexico

Plant Disease ◽  
2000 ◽  
Vol 84 (8) ◽  
pp. 923-923 ◽  
Author(s):  
N. Flores-Estévez ◽  
L. Silva-Rosales ◽  
J. A. Acosta-Gallegos
Keyword(s):  
Bean Common Mosaic Virus ◽  
The State ◽  
Accession Number ◽  
Phaseolus Vulgaris L ◽  
Necrosis Virus ◽  
Chain Reaction ◽  
Bean Plants ◽  
Polymerase Chain ◽  
Alignment Analysis ◽  
Viral Isolates

Bean common mosaic necrosis virus (BCMNV) is a pathogen of Phaseolus vulgaris L. BCMNV was previously classified in serogroup A (for necrotic strains) of the Bean common mosaic virus (BCMV) subgroup; serogroup B included non-necrotic strains of BCMV. Both serogroups are currently classified as two different species in the BCMV subgroup of the Poytvirus genus; strains of either species will produce mosaic symptoms and, in the presence of hypersensitive I gene, necrosis in bean plants (1). Prior to this classification, BCMV was reported in Mexico by the presence of mosaic and necrotic symptoms (2). To investigate the presence of BCMNV in Mexico, samples were collected in two of the main bean producing states. The total extracted RNA was used for reverse transcription polymerase chain reaction using primers complementary to the middle portion of the NIa gene (5') and the 3' UTR of the NL3 Michigan isolate (accession number U19287). Two cDNA segments of 2 and 3 kb were obtained from infected plants from the state of Aguascalientes, in the highlands of central Mexico, and from the state of Veracruz, in the lowlands of the Gulf of Mexico, respectively. The cDNAs were cloned and sequenced. Alignment analysis of these sequences with the NL3 strain of BCMNV showed a similarity of 96.4 and 96.7%, respectively. The similarity between the Aguascalientes (accession number AJ01265) and Veracruz isolates was 99.6%, indicating that both are variants of the same species. On the other hand, alignment analysis of these isolates with some published BCMV strain sequences (i.e., accession numbers L15332 and U55315) displayed low similarities of 52.9 and 64.4%, respectively. These comparisons indicate that the Aguascalientes and Veracruz viral isolates belong to the BCMNV species and is the causal agent of mosaic and necrosis observed on the bean plants in those states. References: (1) C. W. Collmer et al. Mol. Plant-Microbe Interact. 9:758, 1996. (2) E. Jimenez-García. SARH. Mexico. D. F. pp: 3–4, 1994.

scholarly journals Occurrence and distribution of viruses infecting the bean in Serbia

2010 ◽  
Vol 62 (3) ◽  
pp. 595-601 ◽  
Author(s):  
Dragana Petrovic ◽  
Maja Ignjatov ◽  
Zorica Nikolic ◽  
Milka Vujakovic ◽  
Mirjana Vasic ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Viral Infections ◽  
Alfalfa Mosaic Virus ◽  
Bean Common Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Rt Pcr ◽  
Necrosis Virus ◽  
Pcr Method ◽  
Virus Isolates ◽  
Viral Isolates

This work describes the incidence and distribution of the most important bean viruses in Serbia: Bean common mosaic virus (BCMV), Bean common mosaic necrosis virus (BCMNV), Bean yellow mosaic virus (BYMV), Cucumber mosaic virus (CMV) and Alfalfa mosaic virus (AMV). The viral isolates were characterized serologically and biologically. BCMV was found in the largest number of plants (30.53%), followed by BCMNV (2.67%), CMV (5.34%), and AMV (3.41%), since BYMV was not determined. Mixed viral infections were found in several samples. The RT-PCR method was used to prove that the tested isolates belong to the BCMV, family Potyviridae and strains Russian and NL-3 D. Results obtained in this work will enable further studies of the genetic variability of bean virus isolates from Serbia. .

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