Postharvest Treatments with Three Yeast Strains and Their Combinations to Control Botrytis cinerea of Snap Beans

Three yeast strains, namely Cryptococcus albidus (Ca63), Cryptococcus albidus (Ca64), and Candida parapsilosis (Yett1006), and their combinations, including single yeast agent, two combined yeast strains, single yeast agent + NaHCO3, single yeast agent + chitosan, single yeast agent + ascorbic acid, and single yeast agent + konjac powder, were evaluated for their activity against Botrytis cinerea, the most economically important fungal pathogens causing postharvest disease of snap beans. In in vitro tests, no inhibition zone was observed in dual cultures of three yeast strains and B. cinerea. The mycelial growth inhibition rates of B. cinerea for Ca63, Ca64, and Yett1006 were 97%, 95%, and 97%, respectively. In in vivo tests, the optimal combination of the lowest disease index of snap beans with B. cinerea was Ca63 + Ca64, with a preventing effect of 75%. The decay rate and rust spots index of Ca64 + ascorbic acid combination were 25% and 20%, respectively, which were the lowest. The activities of defense-related enzymes increased, while malondialdehyde (MDA) content was suppressed in snap beans after different treatments. Our results highlight the potential of the three yeast strains and their combinations as new nonpolluting agents for the integrated control of B. cinerea on snap beans.

Shrub legume green manure intercropped with maize preceding organic snap bean cultivation

ABSTRACT This study aimed to evaluate strategies for growing pigeon pea and tephrosia intercropped with maize in different spatial arrangements for green manure in organic snap bean farming in succession. The experimental design was a randomized block, in a 2x3 + 1 factorial scheme, corresponding to the two species and the cropping system (monocropping; intercropping with sowing in the same row or between rows). The treatments consisted of pigeon pea and tephrosia monocropping, pigeon pea or tephrosia intercropped with maize in the same planting furrow, pigeon pea or tephrosia intercropped with maize between rows, and maize monocropping (control). After cutting the pre-crops, snap beans were sown. The authors verified that the introduction of shrub leguminous plants in intercropping did not interfere with the phytotechnical characteristics of maize. The tephrosia monocropping and the intercropping of this species with maize, regardless of sowing location, provided, respectively, the highest productivities of pods and dry beans of snap beans, compared with pigeon pea pre-cropping.

Perancangan Cold Storage Untuk Sayuran Buncis Dengan Kapasitas 10 Ton

Abstrak Buncis merupakan salah satu produk pertanian di Indonesia yang diekspor ke luar negeri. Setelah dipanen, buncis disimpan untuk diproses sebelum diekspor ke konsumen. Kesegaran buncis umumnya hanya bertahan selama 1 minggu, oleh karena itu diperlukan alat khusus untuk mempertahankan kesegaran buncis sebelum diekspor ke konsumen. Untuk mempertahankan kesegaran buncis, temperatur udara 4°C-7°C dengan kelembaban 90%-95% perlu dipertahankan. Dengan menggunakan cold strorage, kondisi ruang penyimpanan dapat diatur sedemikian rupa agar memenuhi kriteria tersebut. Pada penelitian ini dirancang sebuah cold storage dengan kapasitas 10 ton untuk tanaman buncis. Cooling Load Temperatur Difference (CLTD) pada perancangan ini diatur bulan dan waktunya yang disesuaikan dengan posisi dari cold storage. Beban pendinginan total untuk 10 ton buncis adalah sebesar 46,73 kW. Cold storage hasil rancangan menggunakan siklus kompresi uap dengan fluida refrigeran R134a tanpa menggunakan humidifier. Untuk mempertahankan kondisi udara pada cold storage agar sesuai dengan kebutuhan, kompressor AC dengan kapasitas 12,7 kW digunakan pada siklus kompresi uap. Performa dari siklus kompresi uap dengan kondisi operasi tersebut ditentukan oleh Coefficient of Performance (COP). Semakin besar nilai COP, maka sistem semakin efisien. Coefficient of Performance (COP) dari siklus tersebut adalah sebesar 3,84. Kata kunci: Buncis, CLTD, Refrigeran, COP, Siklus kompresi uap Abstract Snap beans are one of Indonesian acgricultural product exported to overseas. After harvested, snap beans were stored before exported to consumers. The freshness of the snap beans only lasted for one week, therefor special equipment were required to maintain the snap beans freshness. To maintain the freshness, snap beans must be storage in a room with 4-7°C air temperature and 90-95% humidity. In this research, cold storage was designed for 10 tons of snap beans. Cooling Load Temperature Difference method was used to determine the load of the cold storage based on the position of the building. The total cooling load for 10 tons of snap beans were 46,73 kW. The cold storage was using vapor compression cycle with refrigerant 134a without humidifier . The cycle requires compressor power of 12,7 kW to maintain the condition in the cold storage room. The performance of the cycle was determined from the Coefficient of Performance (COP). The higher value of the COP, the system will be more efficient. The COP of the vapor compression cycle was 3,84. Key words: Snap Beans, Export, Refrigerant, Storage, Humidity.

Phytosanitary and Technical Quality Challenges in Export Fresh Vegetables and Strategies to Compliance with Market Requirements: Case of Smallholder Snap Beans in Kenya

Kenya is one of the leading exporters of snap beans (Phaseolus vulgaris) to Europe, but the export volume has remained below potential mainly due to failure to meet the market quality standards. The quality concerns include the presence of regulated and quarantine pests, pesticide residues, harmful organisms, and noncompliance with the technical standards. These challenges call for the development of alternative approaches in overcoming the phytosanitary and quality challenges in the export of snap beans and other fresh vegetables. These may include integrated pest management (IPM) approaches that incorporate non synthetic chemical options, such as diversified cropping systems, plant and microbial-based pesticides, varieties with multiple disease and pest resistance, insecticidal soaps, pheromones and kairomones, entomopathogens and predators. These approaches, coupled with capacity-building and adherence to the set quality standards, will improve compliance with export market requirements. The aim of this paper is to increase knowledge on implementing good practices across the value chain of fresh vegetables that would lead to improved quality and thereby meeting institutional requirements for the export market. The novelty of the current review is using snap beans as a model vegetable to discuss the challenges that must be mitigated for the quest of achieving high quality and increased volume of fresh export products. Whilst many of the publications have focused on alternatives to synthetic pesticides in addressing MRLs in fresh vegetable exports, there is a disconnect between research and industry in achieving chemical residue and pest free export vegetables. This review describes the phytosanitary and technical challenges faced by smallholder farmers in accessing export markets, evaluates the phytosanitary and quality requirements by the niche markets, and explores the strategies that could be used to enhance compliance to the institutional and market requirements for fresh vegetables.

Expected mean squares.

This chapter focuses on expected mean squares. Expected mean squares are formulas based on statistical theory identifying the components of variability in sources of variation of an ANOVA (analysis of variance) table. Their theoretical derivation is beyond the scope of this text, thus they are presented in a simpler way here, providing a method for deriving expected mean squares without a background in statistical theory. The productivity of 10 cultivars of snap beans was used as an example.

Biofortification efficiency with magnesium salts on the increase of bioactive compounds and antioxidant capacity in snap beans

ABSTRACT: Biofortification of food crops is implemented through the application of mineral fertilizers, to improve the levels of essential mineral elements for human nutrition. Magnesium is a key macronutrient in crop production and quality; however, worldwide, it is the most limiting macronutrient in agriculture. Magnesium plays an important role in manipulating physiological and biochemical processes in plants. Therefore, the objective of this study was to evaluate the efficacy of biofortification with magnesium chloride and sulfate on the accumulation of bioactive compounds and antioxidant capacity in snap bean cv. Strike. Two sources of Magnesium were applied via edaphic route: Magnesium chloride and magnesium sulfate at doses of 0, 50, 100 and 200 ppm during 2018 in Mexico. Accumulation of bioactive compounds (total phenols, total flavonoids and total anthocyanins) and antioxidant capacity in snap bean fruits were evaluated. Results obtained indicate that the concentration of total flavonoids, total anthocyanins and antioxidant capacity in the edible parts of snap beans were higher in the MgSO4 treatment than in the MgCl2 treatments, exceeding 30, 59 and 6% respectively. This is one of the first studies on the edaphic agronomic biofortification of Mg+ and its effect on nutraceutical quality in snap bean. An interesting result is that MgSO4 produced high concentrations of anthocyanins in edible snap beans. These results can be applied as a new strategy to reduce malnutrition and improve the health of the population in poor urban and rural communities in developing countries.

Correlation and path analysis of interaction between snap beans yield and its components with crop management

PURPOSE: To assess the interaction between the applied some agronomic activities for the production of snap beans Phaseolus vulgaris cv. Starozagorski Cher using Path- correlation coefficient of analysis. METHODS: Using path analysis to illustrate real effect of minimizing doses of vegetative herbicide Basagran-200g/l (Bentazon-3-Isopropyl-1H-2,1,3-benzothiadiazin-4(3H)-one 2,2-dioxide) and Fusillade-200g/l (Fluasifop-P-Butil-butyl 2-(4-{[5-(trifluoromethyl)pyridin-2-yl]oxy}phenoxy)propanoate) instead of using Dual 930EK/930g/l-s ( S-metachloras). Collecting data from long years, trials on plants treated with different combinations of foliar suspension fertilizer Lactofol® with pesticides. RESULTS: Growth rate RGR (mg g-1d-1) positively influenced by high statistical significance of the rate of net assimilation NAR– (mg.cm-2d-1). The correlation coefficient between the two indicators is (r = 0.692), followed by the dependence of RGR on the leaf area ratio LAR (cm2 mg-1) with a value of (r = 0.614). High statistical significance ​​of correlation expressing the interaction between the leaf area ratio LAR (cm2 mg-1) and - root weight ratio RWR (g.g-1) (r = 0. 0.837). Leaf area ratio LAR (cm2 mg-1) has a relatively high positive correlation with the rate of net assimilation NAR– (mg.cm-2d-1) (r = 0.508). Results showed that fresh weight of pod/studied variant (+++0.99), fresh weight of one pod (+++0.77), fresh weight of pods/ plant (+++0.67), calcium content in bean pods (++0.57), pods number /plant (++0.51), pollen fertility (++0.44) had positive impact on yield. The partial function of the studied parameters on variability of bean yield is 98.9%. Nitrate content had a major function to the yield (23.3%) followed by Pods/ kg (21.31%).Weeds manifestation caused most negative effect on yield (-0.64). CONCLUSIONS: This study reveals and identifies appropriate changes in applied agricultural techniques in the production of snap beans, which should be paid more attention to decrease water pollution caused by using the soil-applied herbicide without decreasing quantity and quality of yield.

Improvement of Snap Beans Yield through the Application of N, P, K and Granule Organic Fertilizers on Inceptisols Soil

Balanced organic and inorganic fertilization is expected to improve low nutrient on Inceptisols to increased snap bean production. The effect of the combination of N, P, K, and granule organic fertilizer on bean harvests was the purpose of this study. The parameters were leaf area index (LDA), shoot-root ratio, the weight of pods, pod length, pod diameter, percentage of the number of pods, marketable and unmarketable,and percentage of pods by quality class. The experiment was conducted in February to April 2016 at Ciparanje Experimental Farm, Faculty of Agriculture, Padjadjaran Univeristy, Jatinangor. The study was conducted using a randomized block design (RBD) with 10 treatments and 3 replications. The experimental results showed that N, P, K fertilizer and granule organic fertilizer (GOF) in the order of Inceptisols significantly affected the weight of pods. Application 50% of the dosage N, P, K fertilizer combined with 50% dosage of granule organic fertilizer resulted in a higher pod weight per plot, which reached 2 439.84 g. Keywords: an organic fertilizer, granule organic fertilizer, leaf area index, quality grade, shoot-root ratio,

Changes in Sugar Concentrations of Seed and Pod Tissue During Development in Snap and Dry Beans (Phaseolus vulgaris L.)

Sugars, including glucose, fructose, and sucrose, contribute significantly to the flavor and consumer acceptance of snap beans (Phaseolus vulgaris L.). Sugar accumulation and changes in sugar profiles during snap bean development contribute to overall assessments of quality for breeding lines and cultivars. Developing fruit from a diverse group of four snap bean cultivars containing Andean germplasm and one Mesoamerican dry bean cultivar were sampled at 5-day intervals from 10 to 30 days after flowering over 2 years. Glucose, fructose, and sucrose in pod and seed tissue was quantified using high-performance liquid chromatography. Percent seed mass relative to pod mass increased with days after flowering, but the rate of increase was heterogeneous among cultivars. Significant differences in sugar accumulation patterns of mono- and disaccharides were observed with time of development and between pods and seeds. Glucose and fructose decreased rapidly in pods and seeds with time after flowering. In contrast, sucrose concentration increased in pod tissue but remained constant in seeds of the snap bean cultivars with time after flowering. The patterns of changes in pod and seed sugar concentrations with time after flowering were similar among all snap bean cultivars. In contrast to the snap beans, seed sucrose increased with time after flowering in the Mesoamerican dry bean cultivar Puebla 152. No year by day after flowering interactions were observed for sugar accumulation patterns or sugar concentrations. Younger snap beans had the highest sweetness index based on observed sugar concentrations, percent seed mass, and perception of relative sweetness by the human palate. Although mean sweetness varied between cultivars, the rate of decrease in sweetness with time was the same for all five cultivars. These findings indicate that variation for sweetness exists in snap beans and can be exploited by breeding to develop cultivars with a potentially more desirable, sweet flavor.