ISOLASI DAN IDENTIFIKASI Vibrio parahaemolyticus PADA UDANG VANAME (Litopenaeus vannamei) PENYEBAB PENYAKIT VIBRIOSIS

Penelitian ini bertujuan sebagai informasi yang berkaitan dengan isolasi identifikasi Vibrio parahaemolyticus yang dilakukan sebagai langkah dalam menjamin keamanan pangan hasil perikanan sehingga aman untuk di konsumsi. Penelitian ini dilakukan dengan metode ISO/TS 21872-1:2007 langkah yang dilakukan yaitu pre enrichment dengan menggunakan sampel sebanyak 25gram yang dilarutkan dengan menggunakan alkaline saline peptone water 225ml, lalu diinkubasi selama 6jam dengan suhu 41,50C sedangkan untuk sampel segar memerlukan waktu±1 jam, selanjutnya enrichment dengan suhu 41,5 0C selama 18 jam ± 1 jam, selanjutnya dilakukan isolasi serta identifikasi dengan menggunakan media Thiosulfate Citrate Bile Salts Sucrose (TCBS), lau dilakukan inkubasi pada suhu 37 0C selama 24 jam ± 3 jam, setelah itu dilakukan pengamatan dengan ciri-ciri koloni pada V. parahaemolyticus lembut warna hijau (sukrosa negatif) dengan diameter 2-3mm, dan dilakukan uji biokimia dengan melakukan inokulasi pada koloni dengan menggunakan media saline nutrient agar,lalu dilakukan pemeriksaan uji biokimia. Hasil penelitian dari pemeriksaan sampel yang dilakukan di laboratorium Stasiun Karantina Ikan Pengendalian Mutu dan Keamanan Hasil Perikanan Medan II (SKIPM) dapat disimpulkan bahwa dari 7 sampel yang di uji tidak ada yang menunjukkan hasil positif bakteri Vibrio parahaemolyticus dari sampel laut, untuk media dan reagen uji yang digunakan benar dan sesuai dengan pengujian pada kontrol positif bakteri Vibrio parahaemolyticus

Download Full-text

Phage therapy against Vibrio parahaemolyticus infection in the whiteleg shrimp (Litopenaeus vannamei) larvae

Aquaculture ◽

10.1016/j.aquaculture.2014.08.018 ◽

2014 ◽

Vol 434 ◽

pp. 208-211 ◽

Cited By ~ 37

Author(s):

Carlos O. Lomelí-Ortega ◽

Sergio F. Martínez-Díaz

Keyword(s):

Litopenaeus Vannamei ◽

Vibrio Parahaemolyticus ◽

Phage Therapy ◽

Whiteleg Shrimp

Download Full-text

Ocorrência de Vibrio parahaemolyticus em mexilhões (Perna perna, Linnaeus, 1758) de banco natural do litoral do município de Palhoça, Santa Catarina, Brasil

Cadernos de Saúde Pública ◽

10.1590/s0102-311x1994000300017 ◽

1994 ◽

Vol 10 (3) ◽

pp. 379-386 ◽

Cited By ~ 6

Author(s):

Rita Maria B. Archer ◽

Eliane Moretto

Keyword(s):

Vibrio Parahaemolyticus ◽

Bile Salts ◽

Perna Perna ◽

Santa Catarina

Foi estudada a ocorrência de Vibrio parahaemolyticus em quarenta amostras de mexilhões (Perna perna, Linnaeus, 1758) procedentes de banco natural da praia da Pinheira, município de Palhoça, Santa catarina, durante um período de três meses. Na metodologia de isolamento foi utilizada a técnica do Número Mais Provável (NMP) com enriquecimento em água alcalina peptonada e subseqüente plaqueamento em ágar TCBS (thiosulfate citrate bile salts sucrose). Em 52,5% das amostras de mexilhões foi constatada a presença de Vibrio parahaemolyticus, com níveis de contaminação entre <3 e 93 NMP/g. Na caracterização sorológica de 61 culturas, 36,1% não permitiram a identificação das estruturas antigênicas O e K; 54,1% somente da estrutura K e apenas 8,2% tiveram ambas as estruturas definidas. Nenhuma dessas culturas apresentou positividade para o teste de Kanagawa.

Download Full-text

Selectivity and Specificity of a Chromogenic Medium for Detecting Vibrio parahaemolyticus†

Journal of Food Protection ◽

10.4315/0362-028x-68.7.1454 ◽

2005 ◽

Vol 68 (7) ◽

pp. 1454-1456 ◽

Cited By ~ 20

Author(s):

YI-CHENG SU ◽

JINGYUN DUAN ◽

WEN-HSIN WU

Keyword(s):

Vibrio Parahaemolyticus ◽

Bile Salts ◽

Vibrio Vulnificus ◽

Enterobacter Cloacae ◽

Most Probable Number ◽

Chromogenic Medium ◽

Probable Number ◽

Vibrio Fluvialis ◽

Vibrio Mimicus ◽

Vibrio Spp

The thiosulfate–citrate–bile salts–sucrose agar (TCBS) used in the most-probable-number method for detecting Vibrio parahaemolyticus cannot differentiate growth of V. parahaemolyticus from Vibrio vulnificus or Vibrio mimicus. This study examined the selectivity and specificity of Bio-Chrome Vibrio medium (BCVM), a chromogenic medium that detects V. parahaemolyticus on the basis of the formation of distinct purple colonies on the medium. A panel consisting of 221 strains of bacteria, including 179 Vibrio spp. and 42 non-Vibrio spp., were examined for their ability to grow and produce colored colonies on BCVM. Growth of Salmonella, Shigella, Escherichia coli, Enterobacter cloacae, Yersinia enterocolitica, and Aeromonas was inhibited by both BCVM and TCBS. All 148 strains of V. parahaemolyticus grew on BCVM, and 145 of them produced purple colonies. The remaining 31 Vibrio spp., except one strain of Vibrio fluvialis, were either unable to grow or produced blue-green or white colonies on BCVM. Bio-Chrome Vibrio medium was capable of differentiating V. parahaemolyticus from other species, including V. vulnificus and V. mimicus. Further studies are needed to evaluate the sensitivity and specificity of BCVM for detecting V. parahaemolyticus in foods.

Download Full-text

Two host gut-derived lactic acid bacteria activate the proPO system and increase resistance to an AHPND-causing strain of Vibrio parahaemolyticus in the shrimp Litopenaeus vannamei

Developmental & Comparative Immunology ◽

10.1016/j.dci.2018.08.002 ◽

2018 ◽

Vol 89 ◽

pp. 54-65 ◽

Cited By ~ 13

Author(s):

Sudarat Chomwong ◽

Walaiporn Charoensapsri ◽

Piti Amparyup ◽

Anchalee Tassanakajon

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Litopenaeus Vannamei ◽

Vibrio Parahaemolyticus

Download Full-text

Benefits of a Biofilm System and Low Ammonia Levels to Decrees the Early Mortality Syndrome in Shrimp Larvae (Litopenaeus vannamei) Infected with (Vibrio parahaemolyticus)

Asian Journal of Fisheries and Aquatic Research ◽

10.9734/ajfar/2020/v9i330161 ◽

2020 ◽

pp. 31-40

Author(s):

Guillermo Galindo Reyes

Keyword(s):

Litopenaeus Vannamei ◽

Vibrio Parahaemolyticus ◽

Ammonia Concentration ◽

Early Mortality ◽

Shrimp Aquaculture ◽

Economic Losses ◽

Asian Countries ◽

Early Mortality Syndrome ◽

In Series ◽

System Water

The world shrimp aquaculture, has faced several problems, causing severe losses in shrimp hatcheries; between the most critical has been diseases such as early mortality syndrome (EMS) caused by (Vibrio parahaemolyticus). The EMS was initially detected in Asian countries; after, it was disseminated to Mexico and other countries. In Mexico, EMS caused severe economic losses during 2013-2016; and it has not yet been eradicated. Various causes for EMS have been reported; none is entirely accurate, but water quality is essential for successful shrimp aquaculture; therefore, the aim this work was evaluate the ammonia concentration effect on susceptibility to (EMS) on post-larvae (PL-15) shrimp (Litopenaeus vannamei) infected with (V. parahaemolyticus), using a biofilm system (water with, microalgae, dinoflagellates, protozoa and other planktonic microorganisms). So series of 5 flasks each one were arranged as following: Series S; 900 ml of filtered seawater (FSW) and 10 PL-15 shrimp per flask. Series SB; 840 ml of FSW, 60 ml of biofilm and 10 PL-15 shrimp. Series E; 900 ml of FSW, infected with 2 ml (V. parahaemolyticus) 106 CFU/ ml and 10 PL-15 shrimp. Series EN; fifteen flasks with 900 ml of FSW, 10 PL-15 shrimp, added with NH4Cl (0.535 mg/ml), to get 0.5, 1.0, 1.5, 2.0 and 2.5 mg/l final ammonia concentration in 5 sub-series of 3 flasks each one. During experiment, ammonia concentration and PL-15 shrimp mortality were evaluated in all flask. Ammonia concentration was higher in series EN than in series E; the same was observed in Series S respect to SB, but at lower values. At end of experiment, mortality in series EN was 90% Vs 60% in E. Similarly, mortality in series S was 10% Vs 0% in SB. This results confirm that the ammonia increases PL shrimp mortality, and biofilm system reduce ammonia and consequently PL-15 shrimp mortality.

Download Full-text