BINDING PECULIARITIES OF POLY(rA)-POLY(rU) WITH MINOR GROOVE LIGAND HOECHST 33258

2021 ◽  
Vol 55 (2 (255)) ◽  
pp. 144-150
Author(s):  
Marine A. Parsadanyan ◽  
Mariam A. Shahinyan ◽  
Zvart H. Movsisyan ◽  
Ara P. Antonyan
Keyword(s):  
Complex Formation ◽  
Acridine Orange ◽  
Fluorescence Spectra ◽  
Minor Groove ◽  
Hoechst 33258 ◽  
Absorption And Fluorescence Spectra ◽  
Groove Binding

Study on the interaction of DNA-specific ligands – classical intercalator acridine orange (AO) and groove binding compound Hoechst 33258 (H33258) with poly(rA)-poly(rU), being a model for double-stranded (ds-) RNA, has been carried out. The absorption and fluorescence spectra of the complexes of these ligands with ds-polynucleotide were obtained. It was revealed that the optic and fluorescent characteristics of the complexes of both ligands with ds-RNA are similar with those at the complex-formation with DNA.

scholarly journals Impact of Solvents, Magnesium Oxide and Aluminium Oxide Nanoparticles on the Photophysical Properties of Acridine Orange Dye

2021 ◽  
pp. 1-7
Author(s):  
Fairooz Kareem ◽  
Mahasin Al-Kadhemy ◽  
Asrar Saeed
Keyword(s):  
Acridine Orange ◽  
Magnesium Oxide ◽  
Fluorescence Spectra ◽  
Aluminium Oxide ◽  
Blue Shift ◽  
Red Shift ◽  
Oxide Nanoparticles ◽  
Absorption And Fluorescence Spectra ◽  
Aluminium Oxide Nanoparticles ◽  
Al2o3 Nps

Absorption and fluorescence spectroscopy techniques were applied to investigate the photophysical characteristics of acridine orange (AO) dye in solvents that included distilled water, dimethyl sulfoxide (DMSO), acetone and ethanol in various concentrations (1×10-4–1×10-6) M. All of the samples were served at room temperature. The relationships between various parameters describing the strength of optical transitions in atoms and molecules were reviewed. This study expresses various viewpoints by describing how concentration and solvent affect the dye's absorption and fluorescence spectra. The absorption spectra of AO exhibit a band at (490 nm), except for DMSO, which shifts more towards red by 5 nm. The fluorescence spectra show a blue shift in AO aqueous solution around 6 nm until (0.5×10-4) M, followed by a red shift at around 7 nm at (1×10-6) M. There is a blue shift in (1×10-5) M for DMSO at around 4 nm, then a 10 nm red shift in higher concentrations as well as a 9 nm red shift in acetone and 6 nm in ethanol. Adding magnesium oxide nanoparticles (MgO NPs) quenched AO in both absorption and fluorescence spectra, whereas maximum fluorescence and intensity increased when aluminium oxide nanoparticles (Al2O3 NPs) were added to the solution. KEYWORDS Laser dye, absorption spectrum, fluorescence spectrum, MgO NPs, Al2O3 NPs

Drugs and Minor Groove Binding in B-DNA: Netropsin and Hoechst 33258

1987 ◽  
pp. 1-24 ◽  
Author(s):  
M. L. Kopka ◽  
P. E. Pjura ◽  
D. S. Goodsell ◽  
R. E. Dickerson
Keyword(s):  
Minor Groove ◽  
Minor Groove Binding ◽  
Hoechst 33258 ◽  
Groove Binding

ABSORPTION AND FLUORESCENCE SPECTRA OF THE COMPLEXES OF METHYLENE BLUE AND ACRIDINE ORANGE WITH POLY(rA)-POLY(rU)

2021 ◽  
Vol 55 (2 (255)) ◽  
pp. 151-157
Author(s):  
Mariam A. Shahinyan ◽  
Zvart H. Movsisyan ◽  
Gohar G. Hovhannisyan ◽  
Poghos H. Vardevanyan
Keyword(s):  
Methylene Blue ◽  
Fluorescence Spectroscopy ◽  
Acridine Orange ◽  
Fluorescence Spectra ◽  
Isosbestic Point ◽  
Absorption And Fluorescence Spectra ◽  
Absorption And Fluorescence Spectroscopy ◽  
Ru Complexes

The interaction of methylene blue (MB) and acridine orange (AO) with poly(rA)-poly(rU) was studied using absorption and fluorescence spectroscopy methods. The absorption and fluorescence spectra of complexes of these ligands with a polynucleotide were obtained, similar to those of complexes of these ligands with double-stranded (ds-) DNA. It was revealed that the isosbestic point in the spectra of the AO-poly(rA)-poly(rU) and MB-poly(rA)-poly(rU) complexes is not formed, although the binding of AO and MB with ds-RNA by intercalation mode is not excluded.

Minor-Groove Binding Drugs: Where Is the Second Hoechst 33258 Molecule?

2013 ◽  
Vol 117 (19) ◽  
pp. 5820-5830 ◽  
Author(s):  
Louise H. Fornander ◽  
Lisha Wu ◽  
Martin Billeter ◽  
Per Lincoln ◽  
Bengt Nordén
Keyword(s):  
Minor Groove ◽  
Minor Groove Binding ◽  
Hoechst 33258 ◽  
Groove Binding
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