scholarly journals DETERMINATION OF NICOTINE IN DOMESTIC AND IMPORTED CIGARETTES IN ADEN (YEMEN) MARKETS

2021 ◽  
Vol 2 (3) ◽  
pp. 131-138
Author(s):  
Ahmed Thabet Ahmed Alsarahe ◽  
Bassam Nasr Mgammal Sanad
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Statistical Analysis ◽  
High Performance ◽  
Uv Detector ◽  
International Standard ◽  
Nicotine Content

The current study aimed at determining the nicotine content of some domestic and imported cigarettes and comparing the nicotine content among 12 types of familiar cigarettes sold in the markets of Aden Governorate (Yemen) using high-performance liquid chromatography linked with a UV detector, (HPLC-UV). The results of this study showed that the amount of nicotine in each cigarette was from 7.29 to 18.74 mg/g cigarettes with an average of 12.75mg/g cigarettes, in imported cigarettes, and between 11.34 to 17.52 mg/g cigarettes with an average of 14.40 mg/g in domestic cigarettes. Although the amount of nicotine in both domestic and imported cigarettes showed a large variation, but when comparing the averages of local and imported cigarettes, the statistical analysis showed that there were no statistically significant differences between the averages of local and imported cigarettes. The Nicotine content of all tested cigarettes (imported and local brands) was higher than the international standard (1mg/g tobacco).

Determination of Mimosine and 2,3-Dihydroxypyridine in Leucaena leucocephala by Reversed Phase High-Performance Liquid Chromatography

2011 ◽  
Vol 140 ◽  
pp. 296-301 ◽  
Author(s):  
Cai Mei Wu ◽  
Hong Min Yuan ◽  
Gang Jia ◽  
Zhi Sheng Wang ◽  
Xiu Qun Wu
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Leucaena Leucocephala ◽  
High Performance ◽  
Limit Of Detection ◽  
Reversed Phase ◽  
Quantitative Detection ◽  
Uv Detector ◽  
Chromatography Method

A reversed high performance liquid chromatography method was developed for the quantitative determination of mimosine and 2,3-DHP in leaves ofLeucaena Leucocephala. Mimosine and 2,3DHP were extracted using 0.1N HCl.The chromatograph conditions were investigated and optimized. The optimal HPLC conditions as follows: Agilent HC-C18 column （4.6×150mm,5μm） was used at 30°C. The method used a variable wavelength UV detector at 280nm, the mobile phase consisted of 0.2 % (w/v) orthophosphoric acid and methanol, the gradient elution was adopted. The injection volume was 10μL. The linearity is favorable in the range of 1.0 to 50μg mL-1with a correlation coefficient of 0.99998 for mimosine and 0.99902 for 2,3DHP. Under the optimal conditions, the method limit of detection (LOD) of mimosine and 2,3DHP were 0.40mg/kg and 0.55mg/kg respectively. The recovery of mimosine was 87.00-94.70% with the RSD (n=5) of 2.75-3.81% in the spiked levels 0,1, 5, 20mg/g. At the same time, the recovery of 2,3DHP was 88-95.4% with the RSD (n=5) of 2.24-4.90%. The method was found to be simple, sensitive, fast and accurate, and has been applied successfully for the quantitative detection of mimosine and 2,3-DHP in leaves ofLeucaena Leucocephala, plasma and excretion of ruminant.

scholarly journals Validation and statistical analysis of two high performance liquid chromatography methods for the determination of indinavir sulfate raw material and capsules

Química Nova ◽  
2005 ◽  
Vol 28 (1) ◽  
pp. 50-53 ◽  
Author(s):  
Breno de Carvalho e Silva ◽  
Lígia Maria Moreira de Campos ◽  
Elzíria Aguiar Nunan ◽  
Cristina Duarte Vianna Soares ◽  
Gisele Rodrigues Silva ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Statistical Analysis ◽  
High Performance ◽  
Raw Material ◽  
Indinavir Sulfate

scholarly journals Certification of amburana in the aging of cachaça

2020 ◽  
Vol 9 (12) ◽  
pp. e3891210644
Author(s):  
Amazile Biagioni Maia ◽  
Lorena Simão Marinho ◽  
David Lee Nelson
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Rapid Method ◽  
High Performance ◽  
Aging Process ◽  
Uv Detector ◽  
Standard Curve ◽  
Chromatographic Profile ◽  
Phenolic Derivatives

Cachaça is defined as sugarcane spirit produced in Brazil, but it still lacks references that valorize the aging in native woods. Amburana (Amburana cearensis) is a Brazilian tree widely used in making casks for storing cachaça. Among its phenolic derivatives, coumarin stands out. Its content is determinant in the sensorial characteristics developed in the beverage during the aging process. We propose a simple and rapid method (15 min) that allows the certification of the storage of cachaça in amburana barrels and the determination of the coumarin content. Using high-performance liquid chromatography, the identity of the wood is attested by the peculiarities of the chromatographic profile obtained with a UV detector (274 nm) in an isothermal environment (40 oC). Coumarin was easily identified among the peaks obtained, and it could be quantified using a standard curve.

scholarly journals High-performance liquid chromatography for determination of α-tocopherol in vegetables

2013 ◽  
Vol 42 (1-2) ◽  
pp. 197-205 ◽  
Author(s):  
Marcin Horbowicz
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Chromatographic Determination ◽  
Uv Detector ◽  
Elution Time ◽  
Simple Method ◽  
Silica Column ◽  
Minimum Detectable Amount

A simple method for the determination of α-tocopherol in vegetables is described. The procedure consists of the following steps: saponification, extraction, silica-column clean-up, and high-performance liquid chromatography. Elution time for D, L-α-tocopherol was 9.0 min using a Zorbax Sil (250 x 4.6 mm) column and an isocratic mobile phase of hexane-methanol (99.3 + 0.7), with a flow rate of 1 ml/min, and detection at 292 nm using a variable UV detector. The average recovery of α-tocopherol was 91.2%, and the minimum detectable amount was 0.1 mg/100 g of fresh vegetable tissue. This method is comparable to gas-chromatographic determination of α-tocopherol, but has fewer analytical steps and gives more reproducible results.

Analysis of Sesquiterpene Lactones by High Performance Liquid Chromatography

1980 ◽  
Vol 35 (11-12) ◽  
pp. 915-918 ◽  
Author(s):  
Dieter Strack ◽  
Peter Proksch ◽  
Paul-Gerhard Gülz
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Crude Extract ◽  
High Performance ◽  
Stationary Phases ◽  
Sesquiterpene Lactones ◽  
Gradient Elution ◽  
Uv Detector ◽  
Laurus Nobilis

High performance liquid chromatography on octyl- and octadecyl-silica (RP-8 and RP-18) stationary phases offers an efficient and rapid method for analysis of sesquiterpene lactones. A mixture of 11 lactones was analyzed using gradient elution with increasing acetonitrile concentra­tion in water and monitoring the column effluent at 210 nm with an UV detector. The described method has been applied to the determination of costunolide in a crude extract from leaves of Laurus nobilis.

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