scholarly journals Application of chromato-mass-spectrometric methods of determination of exposure markers in biomonitoring researches in workers of productions of polyvinyl chloride and aluminum

2020 ◽  
Vol 99 (10) ◽  
pp. 1159-1164
Author(s):  
Salim F. Shayakhmetov ◽  
Olga M. Zhurba ◽  
Anton N. Alekseenko ◽  
Alexey V. Merinov
Keyword(s):  
Polyvinyl Chloride ◽  
Mass Spectrometric ◽  
Aluminum Production ◽  
Control Groups ◽  
Biological Media ◽  
Mass Selective Detector ◽  
Selective Detector ◽  
Highly Sensitive ◽  
Polycyclic Aromatic

Introduction. The presence of highly toxic polycyclic aromatic hydrocarbons (PAHs) in the production of aluminum and organochlorine compounds (OCC) in the production of polyvinyl chloride (PVC) poses a serious threat to the health of workers, necessitates biological monitoring of toxicants and their metabolites in biological media to assess health risks on basis of modern methods chemical analysis. Material and methods. Biomonitoring studies of the content of marker metabolite OCC - thiodiacetic acid (TDAA) in urine were performed in 65 workers of PVC production and PAHs metabolite - 1-hydroxypyrene (1-OHPyr) in 144 workers of the aluminum smelter using developed own methods of gas-chromato-mass-spectrometric (GC-MS) analysis. Sample analysis was performed on an Agilent 7890A gas chromatograph with an Agilent 5979 mass selective detector. Results. Methodological methods and parameters of GC-MS measurement of TDAA and 1-OHPyr in urine, which provided high selectivity and sensitivity of the analysis of samples, were examined and considered. Reliable differences in the levels of marker metabolites among the groups of main and auxiliary occupations and persons in the control groups, their dependence on the degree of exposure to PAHs, and OCC were established. The highest concentrations of TDAA in urine were observed in unit operators of PVC workshop and 1-OHPyr - in anode workers of aluminum production. Discussion. The revealed reliable intergroup and interindividual differences in the contents of TDAA and 1-OHPyr in the urine of workers indicate the reliability and informativeness of the results of the analysis of biological media. The results are consistent with data from foreign studies, confirm the occupational - production nature of the exposure of toxicants among workers in the main professions of enterprises. Conclusion. The results of approbation of highly sensitive and selective methods for the determination of TDAA and 1-OHPyr in urine samples developed on the base of GC-MS method demonstrate the possibility of their use in biomonitoring studies of workers of productions and the population to adequately assess the exposure of highly toxic PAHs and OCC.

scholarly journals Determina tion of diacetyl and 2,3-pentanedione in beer by gc/ms using solid-phase extraction columns

2002 ◽  
pp. 45-54 ◽  
Author(s):  
Jelena Pejin ◽  
Olgica Grujic ◽  
Nikola Marjanovic ◽  
Djura Vujic ◽  
Suncica Kocic-Tanackov
Keyword(s):  
Gas Chromatography ◽  
Solid Phase Extraction ◽  
Simultaneous Determination ◽  
Solid Phase ◽  
Phase Extraction ◽  
Mass Selective Detector ◽  
Selective Detector

A new GC/MS method for the determination of diacetyl and 2,3-pentanedione was investigated. Diacetyl and 2,3-pentanedione were derivatized with 1,2-diaminobenzene to form 2,3-dimetylquinoxaline and 2-ethyl-3-methylquinoxaline. respectively. The amounts of formed 2.3-dimetylqu:inoxaline and 2-ethyl-3,.methylquinoxaline were proportional to the concentrations of diacetyl and 2,3-penianedione present in the sample. 2,3-Dimetylquinoxaline and 2-ethyl-3-methylquinoxaline were extracted by solid-phase extraction (SPE) columns and determined by gas chromatography using a mass selective detector. This method was applied for the determination of diacetyl and 2,3-pentanedione concentrations in beer. Extraction by SPE columns proved to be very simple and reliable. The method can be used for simultaneous determination of diacetyl and 2,3-pentanedione concentrations in beer in a great number of beer samples.

Determination of Twenty-one Chloroanisoles in Water and Sediment Samples

1988 ◽  
Vol 71 (4) ◽  
pp. 803-807
Author(s):  
Hing-Biu Lee
Keyword(s):  
Molecular Ions ◽  
Sediment Samples ◽  
Mass Selective Detector ◽  
Water And Sediment ◽  
Soxhlet Apparatus ◽  
Selective Detector ◽  
Electron Impact Mass ◽  
Impact Mass ◽  
Water And Sediment Samples

Abstract A simple and sensitive method for the determination of 19 chloroanisoles and 2 chloromethylanisoles was developed for water; another method was developed for sediment samples. Water samples were extracted with dichloromethane; sediments were extracted with a mixture of hexane and acetone in a Soxhlet apparatus. The extracts were concentrated on a Snyder column and then were cleaned up on an activated Florisil column. The anisoles were separated by either an OV-1 or SPB-5 capillary column and were detected by an electroncapture or a mass selective detector. Recoveries of chloroanisoles in fortified water and sediment samples generally ranged between 70 and 85%. The method detection limits were 0.02 /tg/L and 0.002 Mg/g for mono- and di-chloroanisoles in water and sediments and 0.002 jug/L and better than 0.001 jig/g for tri-, tetra-, and pentachloroanisoles in those matrixes. The electron-impact mass spectra of all chloroanisoles exhibited intense peaks for the molecular ions (M+ ), as well as (M - 43)+ and either (M - 15)+ or (M - 30)+ fragments. These masses were used as characteristic ions for quantitative and confirmation purposes.

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