Molecular subtyping and phylogeny of Blastocystis sp. isolated from turkey (Meleagris gallopavo) populations in Penang, Malaysia

Background: The aim of the present study is to describe the occurrence of Blastocystis sp. detection among asymptomatic subjects and patients with irritable bowel syndrome in order to evaluate the potential association between irritable bowel syndrome and the parasitic infection. Methods: Cross-sectional study where adult patients with irritable bowel syndrome diagnosed according to Rome IV criteria were included. A control group was formed by asymptomatic subjects older than 18 years. Exclusion criteria were: immunosuppressive condition or having received any drug with demonstrated activity against Blastocystis sp. within the last 6 months before study inclusion. Epidemiological and clinical information was collected from all included participants. Two stool samples were obtained from all participants: one sample for microscopic examination and one sample for Blastocystis sp. PCR detection. Blastocystis sp. infection was defined by the positivity of any of the diagnostic techniques. Results: Seventy-two participants were included (36 asymptomatic subjects and 36 patients with irritable bowel syndrome). Thirty-five (48.6%) were men, and median age of participants was 34 (IQR 29–49) years. The overall rate of Blastocystis sp. carriage was 27.8% (20/72). The prevalence assessed through microscopic examination was 22.2% (16/72), while the prevalence measured by PCR was 15.3% (11/72). When comparing the presence of Blastocystis sp. between asymptomatic subjects and IBS patients, we did not find any statistically significant difference (36.1% vs. 19.4% respectively, p = 0.114). Conclusions: regarding the occurrence of Blastocystis sp., no differences were found between asymptomatic participants and patients with irritable bowel disease irrespective of the diagnostic technique performed.

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Molecular identification and subtyping of Blastocystis sp. in hospital patients in Central China

European Journal of Protistology ◽

10.1016/j.ejop.2021.125796 ◽

2021 ◽

pp. 125796

Author(s):

Junqiang Li ◽

Haiju Dong ◽

Md Robiul Karim ◽

Xianli Yang ◽

Liqin Chao ◽

...

Keyword(s):

Molecular Identification ◽

Central China ◽

Hospital Patients ◽

Blastocystis Sp

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Molecular Subtyping in the Neoadjuvant Setting in Prostate Cancer: Envisioning the Possibilities

European Urology ◽

10.1016/j.eururo.2021.04.029 ◽

2021 ◽

Author(s):

Heather H. Cheng

Keyword(s):

Prostate Cancer ◽

Molecular Subtyping

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Survey of a cDNA library from the turkey (Meleagris gallopavo)

Genome ◽

10.1139/g04-088 ◽

2005 ◽

Vol 48 (1) ◽

pp. 12-17 ◽

Cited By ~ 14

Author(s):

L D Chaves ◽

J A Rowe ◽

K M Reed

Keyword(s):

Single Nucleotide Polymorphism ◽

Expressed Sequence Tags ◽

Expressed Sequence Tag ◽

Meleagris Gallopavo ◽

Whole Genome Sequence ◽

Snp Discovery ◽

Nucleotide Polymorphism ◽

Single Nucleotide ◽

Important Species ◽

Expressed Sequence

Genome characterization and analysis is an imperative step in identifying and selectively breeding for improved traits of agriculturally important species. Expressed sequence tags (ESTs) represent a transcribed portion of the genome and are an effective way to identify genes within a species. Downstream applications of EST projects include DNA microarray construction and interspecies comparisons. In this study, 694 ESTs were sequenced and analyzed from a library derived from a 24-day-old turkey embryo. The 437 unique sequences identified were divided into 76 assembled contigs and 361 singletons. The majority of significant comparative matches occurred between the turkey sequences and sequences reported from the chicken. Whole genome sequence from the chicken was used to identify potential exon–intron boundaries for selected turkey clones and intron-amplifying primers were developed for sequence analysis and single nucleotide polymorphism (SNP) discovery. Identified SNPs were genotyped for linkage analysis on two turkey reference populations. This study significantly increases the number of EST sequences available for the turkey.Key words: turkey, cDNA, expressed sequence tag, single nucleotide polymorphism.

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Cryptosporidium hominis genotypes involved in increased incidence and clusters of cases, Navarra, Spain, 2012

Epidemiology and Infection ◽

10.1017/s0950268814001836 ◽

2014 ◽

Vol 143 (5) ◽

pp. 1033-1036 ◽

Cited By ~ 2

Author(s):

I. FUENTES ◽

C. MARTÍN ◽

X. BERISTAIN ◽

A. MAZÓN ◽

J. M. SAUGAR ◽

...

Keyword(s):

Urban Area ◽

Rural Area ◽

The Other ◽

Single Exposure ◽

Cryptosporidium Hominis ◽

Molecular Subtyping ◽

The Future

SUMMARYTwo clusters of confirmed cryptosporidiosis infections were detected in Navarra, Spain, in the summer of 2012, in the context of an increased incidence in the region. Molecular subtyping of Cryptosporidium hominis determined that one cluster, occurring in an urban area, was due to the predominant circulating subtype IbA10G2R2 and the other cluster, with cases occurring in a rural area, was due to a rare subtype IaA18R3. No single exposure was associated with infection, although exposure to certain children's pools was reported by a majority of patients interviewed in each cluster. Genotyping tools were useful in the investigation and could aid investigation of cryptosporidiosis outbreaks in Spain in the future.

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