scholarly journals HPV genotyping and p16/Ki-67 test significantly improve detection rate of high-grade cervical squamous intraepithelial lesion

2020 ◽  
Vol 16 (1) ◽  
pp. 87-93
Author(s):  
Piotr Lewitowicz ◽  
Anna Nasierowska-Guttmejer ◽  
Wojciech Rokita ◽  
Olga Adamczyk-Gruszka ◽  
Stanisław Gluszek ◽  
...  
Keyword(s):  
Detection Rate ◽  
High Grade ◽  
Squamous Intraepithelial Lesion ◽  
Ki 67 ◽  
Hpv Genotyping ◽  
Intraepithelial Lesion ◽  
Cervical Squamous Intraepithelial Lesion

scholarly journals Biomarker Expression in Multifocal Vulvar High-Grade Squamous Intraepithelial Lesions

Cancers ◽  
2021 ◽  
Vol 13 (22) ◽  
pp. 5646
Author(s):  
Nikki B. Thuijs ◽  
Willemijn A. M. Schonck ◽  
Linde L. J. Klaver ◽  
Guus Fons ◽  
Marc van Beurden ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Expression Profiles ◽  
High Grade ◽  
Squamous Intraepithelial Lesion ◽  
Predictive Diagnostics ◽  
Ki 67 ◽  
Intraepithelial Lesion ◽  
Prospective Longitudinal ◽  
Intraepithelial Lesions ◽  
Six Genes

In patients with high-grade squamous intraepithelial lesion (HSIL) of the vulva, the presence of multiple lesions, called multifocal HSIL, is common. The aim of this exploratory study was to investigate biomarker expression profiles in multifocal HSIL. In total, 27 lesions from 12 patients with high-risk human papillomavirus (HPV)-positive multifocal HSIL were tested for HPV genotype, expression of p16INK4a and Ki-67, and DNA methylation of six genes. HPV16 was found most commonly in 21 (77.8%) HSILs. In two (16.4%) patients, HPV genotype differed between the lesions. All lesions demonstrated diffuse p16INK4a staining, of which three (11.1%) were combined with patchy staining. One patient (8.3%) demonstrated markedly different DNA methylation levels between lesions. Generally, heterogeneity in methylation profiles was observed between different patients, even when other biomarkers showed similar expression. In conclusion, this study is the first to demonstrate heterogeneity of individual lesions in patients with multifocal HSIL. The studied biomarkers have the potential to refine prognostic and predictive diagnostics. Future prospective, longitudinal studies are needed to further explore the potential of a biomarker profile for management of patients with multifocal HSIL.

scholarly journals The utility of p16INK4aand Ki-67 to identify high-grade squamous intraepithelial lesion in adolescents and young women

2012 ◽  
Vol 55 (3) ◽  
pp. 339 ◽  
Author(s):  
José Eleutério ◽  
DianeM Cavalcante ◽  
IaraM Linhares ◽  
MargaridaM.L Pompeu ◽  
PauloC Giraldo
Keyword(s):  
Young Women ◽  
High Grade ◽  
Squamous Intraepithelial Lesion ◽  
Ki 67 ◽  
Intraepithelial Lesion

p16 and Ki-67 Immunostaining in Atypical Immature Squamous Metaplasia of the Uterine Cervix: Correlation With Human Papillomavirus Detection

2007 ◽  
Vol 131 (9) ◽  
pp. 1343-1349 ◽  
Author(s):  
Lori Iaconis ◽  
Elizabeth Hyjek ◽  
Lora H. Ellenson ◽  
Edyta C. Pirog
Keyword(s):  
Polymerase Chain Reaction ◽  
Squamous Metaplasia ◽  
Human Papillomaviruses ◽  
High Grade ◽  
Squamous Intraepithelial Lesion ◽  
Chain Reaction ◽  
Ki 67 ◽  
Intraepithelial Lesion ◽  
Polymerase Chain ◽  
Cervical Mucosa

Abstract Context.—Atypical immature squamous metaplasia (AIM) of the cervix is a loosely defined entity characterized by immature metaplastic cells with mild cytologic atypia. Objective.—To examine whether a combination of immunostaining for p16 and Ki-67 could be used to stratify AIM cases into 3 categories: benign, cases with nondiagnostic atypia, and high-grade squamous intraepithelial lesion (HSIL). Design.—The study consisted of 37 cases of AIM, 23 cases of benign cervical mucosa (NEG), and 36 cases of HSIL. All cases were tested for high-risk human papillomaviruses using SPF 10 polymerase chain reaction and immunostained for p16 and Ki-67. Results.—All cases of HSIL were positive for both p16 and Ki-67. All but 2 benign control cases were negative for both p16 and Ki-67. Seven cases of AIM (19%) displayed a pattern of immunostaining identical to HSIL, and these most likely represent a spectrum of HSIL. A total of 54% of cases of AIM were negative for both p16 and Ki-67, consistent with benign reactive atypia. Two AIM cases (5%) were negative for p16 and positive for Ki-67 in the area adjacent to an ulcer, representing regeneration. Finally, 22% of AIM cases were positive for p16 and negative for Ki-67; such cases may represent a precursor of HSIL or, alternatively, a regressing HSIL. Conclusion.—The combination of immunostaining for p16 and Ki-67 is helpful in limiting of the number of cases with nondiagnostic atypia of the cervix.

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