Efficacy of Platelet-Rich Plasma on Fat Grafts in the Repair of Tympanic Membrane Perforations: An Experimental Study

We conducted a prospective study to assess the effectiveness of a platelet-rich plasma hourglass graft in the repair of small tympanic membrane perforations as an office-based procedure. Our study population was made up of 25 patients—10 men and 15 women, aged 19 to 45 years (mean: 30.4 ± 7.2)—who each underwent repair of one eardrum. After administration of topical anesthesia, a single piece of platelet-rich plasma approximately double the size of the perforation was obtained from each patient's intravenous blood sample. After the margin of the perforation was freshened, the platelet-rich plasma was placed in an hourglass configuration, with equal portions lying medial and lateral to the perforation. Successful perforation repair was achieved in 21 of the 25 ears (84%). No patient developed an infection, hearing impairment, tinnitus, vertigo, bleeding, taste disturbance, or hyperkeratosis. We conclude that office-based platelet-rich plasma myringoplasty is a safe and effective minimally invasive procedure that is suitable for repairing small tympanic membrane perforations.

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Abstract P2-14-04: Is the combination of fat grafts and platelet rich plasma effective and safe? An experimental study in rats

10.1158/1538-7445.sabcs14-p2-14-04 ◽

2015 ◽

Author(s):

Ruffo Freitas-Junior ◽

Alexandre R Blumenschein ◽

Marise AR Moreira ◽

Maria-Auxiliadora PC Cysneiros ◽

Roseana N Pereira

Keyword(s):

Experimental Study ◽

Platelet Rich Plasma ◽

Fat Grafts

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Mastoid Reconstruction With Platelet-Rich Plasma and Bone Pate After Canal Wall Down Mastoidectomy: A Preliminary Report

Ear Nose & Throat Journal ◽

10.1177/0145561319879789 ◽

2019 ◽

pp. 014556131987978 ◽

Cited By ~ 1

Author(s):

Sherif M. Askar ◽

Ibrahim M. Saber ◽

Mohammad Omar

Keyword(s):

Tympanic Membrane ◽

Cortical Bone ◽

Platelet Rich Plasma ◽

Case Series ◽

Stressful Events ◽

Mastoid Cavity ◽

Canal Wall ◽

Tympanic Membrane Perforations ◽

Canal Wall Down ◽

Canal Wall Down Mastoidectomy

Objectives: Mastoid reconstruction principle had been described to overcome problems of chronic discharging cavity. Different materials were used; nonbiologic materials seem to be less preferred. Platelet-rich plasma (PRP) could promote the regeneration of mineralized tissues. In this work, the authors present a simple and easy technique for mastoid reconstruction with PRP and cortical bone pate. Methods: The study design is a case series. Patients had mastoid reconstruction after canal wall down mastoidectomy using PRP and cortical bone pate. Results: This study included 21 patients: 9 males, and 12 females. Sixteen patients had left side disease. All surgical procedures were conducted smoothly within 90 to 135 minutes with no stressful events had been reported. At 12 to 16 months of follow-up, external canal stenosis and mastoid fistulas were not reported. Good healing of the tympanic membrane was seen in 18 patients. No radiological signs suggestive of recurrence were detected and the reconstructed mastoid cavity was smooth and well aerated. Residual tympanic membrane perforations were detected in 3 patients. Conclusion: Autologous materials (PRP and bone pate pate) after canal wall down mastoidectomy appear to be a reliable and effective choice for mastoid reconstruction.

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Platelet-rich plasma improves healing of tympanic membrane perforations: experimental study

The Journal of Laryngology & Otology ◽

10.1017/s0022215108003848 ◽

2008 ◽

Vol 123 (5) ◽

pp. 482-487 ◽

Cited By ~ 23

Author(s):

E Erkilet ◽

M Koyuncu ◽

S Atmaca ◽

M Yarim

Keyword(s):

Tympanic Membrane ◽

Histopathological Examination ◽

Platelet Rich Plasma ◽

Healing Time ◽

Tympanic Membrane Perforation ◽

Control Group ◽

Tympanic Membrane Perforations ◽

Significant Difference ◽

Membrane Perforation ◽

Tympanic Membranes

AbstractObjective:The aim of this study was to investigate the effect of local application of platelet-rich plasma to perforated rat tympanic membranes, in terms of healing time and histopathological outcome.Methods:Eighty-eight tympanic membranes of 44 rats were given a standard 3 mm perforation, and platelet-rich plasma was applied to the right tympanic membrane perforations. The left tympanic membranes were left to heal spontaneously, as controls. The 44 rats were divided into two groups. In group one, comprising 20 rats, daily otomicroscopic examination of the tympanic membrane perforations was performed. The 24 rats in group two were subdivided into four subgroups of six rats each; these subgroups were sacrificed sequentially on days three, seven, 14 and 28 for histopathological examination, regardless of tympanic membrane healing stage.Results:In group one, the mean tympanic membrane healing times for tympanic membrane perforations receiving platelet-rich plasma and controls were respectively 10.2 ± 2.1 and 13.0 ± 2.9 days (mean ± standard deviation). This difference was statistically significant (p < 0.001). In group two, histopathological evaluation of tympanic membrane perforation healing at days three, seven, 14 and 28 did not reveal any statistically significant difference, individually or within the four groups as a whole.Conclusion:These findings suggest that earlier healing of tympanic membrane perforations occurred in the platelet-rich plasma group compared with the control group. These findings suggest that platelet-rich plasma is effective in accelerating tympanic membrane perforation healing, and that it may be effective in human subjects, particularly as it is an autologous material.

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Platelet-Rich Plasma Fat Graft versus Cartilage Perichondrium for Repair of Medium-Size Tympanic Membrane Perforations

Otolaryngology ◽

10.1177/0194599818789146 ◽

2018 ◽

Vol 160 (1) ◽

pp. 116-121 ◽

Cited By ~ 3

Author(s):

Mahmoud Fawzy Mandour ◽

Mohamed Nasser Elsheikh ◽

Mohamed Fathy Khalil

Keyword(s):

Tympanic Membrane ◽

Platelet Rich Plasma ◽

Neck Surgery ◽

Tympanic Membrane Perforation ◽

Fat Graft ◽

Controlled Study ◽

Medium Size ◽

Tympanic Membrane Perforations ◽

Group 2 ◽

Group 1

Objectives To compare clinical and audiologic outcomes among patients who underwent myringoplasty with fat graft enriched with platelet-rich plasma and cartilage perichondrium graft. Study Design Prospective randomized controlled study. Setting Department of Otolaryngology–Head and Neck Surgery, Tanta University, Egypt. Subjects and Methods The study comprised 50 patients who had tympanic membrane perforation of medium size (25%-50% of its surface area) without evidence of active otitis media or ossicular abnormalities and who had surgery between December 2016 and December 2017. Patients randomly underwent myringoplasty with fat graft enriched with platelet-rich plasma (group 1) or cartilage perichondrium graft (group 2). Results Twenty-five patients were included in each group. Rates of successful closure at 3-month follow-up were 88% (group 1) and 92% (group 2). The mean overall improvement in pure tone average was 18.08 dB (95% CI, 16.9-19.25) for group 1 and 18.24 dB (95% CI, 16.94-19.53) for group 2. Conclusion Fat graft enriched with platelet-rich plasma can be recommended as an alternative choice for first-line treatment of medium-size central perforations of the tympanic membrane, with healing and hearing results comparable to those of conventional cartilage perichondrium myringoplasty.

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