Abstract. Secondary Organic Aerosol (SOA) particles have been found to be efficient ice nucleating particles under the cold conditions of (tropical) upper tropospheric cirrus clouds. Whether they also are efficient at initiating freezing at slightly warmer conditions as found in mixed phase clouds remains undetermined. Here, we study the ice nucleating ability of photo-chemically produced SOA particles with the combination of the Manchester Aerosol and Ice Cloud Chambers. Three SOA systems were tested resembling biogenic/anthropogenic particles and particles of different phase state. After the aerosol particles were formed, they were transferred into the cloud chamber where subsequent quasi-adiabatic cloud evacuations were performed. Additionally, the ice forming abilities of ammonium sulfate and kaolinite were investigated as a reference to test the experimental setup. Clouds were formed in the temperature range of −20 °C to −28.6 °C. Only the reference experiment using dust particles showed evidence of ice nucleation. No ice particles were observed in any other experiment. Thus, we conclude that SOA particles produced under the conditions of the reported experiments are not efficient ice nucleating particles starting at liquid saturation under mixed-phase cloud conditions.