Supplementary material to "Environmental DNA simultaneously informs hydrological and biodiversity characterization of an Alpine catchment"

2020 ◽  
Author(s):  
Elvira Mächler ◽  
Anham Salyani ◽  
Jean-Claude Walser ◽  
Annegret Larsen ◽  
Bettina Schaefli ◽  
...  
Keyword(s):  
Environmental Dna ◽  
Supplementary Material

Catalytic Fluorination of Boron Compounds at a Bismuth Redox Platform

2019 ◽  
Author(s):  
Oriol Planas ◽  
Feng Wang ◽  
Markus Leutzsch ◽  
Josep Cornella
Keyword(s):  
Transition Metal ◽  
Main Group ◽  
Group Element ◽  
Oxidation States ◽  
Boron Compounds ◽  
Main Text ◽  
Main Group Element ◽  
Rational Ligand Design ◽  
Supplementary Material

The ability of bismuth to maneuver between different oxidation states in a catalytic redox cycle, mimicking the canonical organometallic steps associated to a transition metal, is an elusive and unprecedented approach in the field of homogeneous catalysis. Herein we present a catalytic protocol based on bismuth, a benign and sustainable main-group element, capable of performing every organometallic step in the context of oxidative fluorination of boron compounds; a territory reserved to transition metals. A rational ligand design featuring hypervalent coordination together with a mechanistic understanding of the fundamental steps, permitted a catalytic fluorination protocol based on a Bi(III)/Bi(V) redox couple, which represents a unique example where a main-group element is capable of outperforming its transition metal counterparts.<br>A main text and supplementary material have been attached as pdf files containing all the methodology, techniques and characterization of the compounds reported.<br>

scholarly journals Assessing high-throughput environmental DNA extraction methods for meta-barcode characterization of aquatic microbial communities

2018 ◽  
Vol 17 (1) ◽  
pp. 37-49 ◽  
Author(s):  
Abdolrazagh Hashemi Shahraki ◽  
Subba Rao Chaganti ◽  
Daniel Heath
Keyword(s):  
Microbial Community ◽  
Microbial Communities ◽  
High Throughput ◽  
Environmentally Friendly ◽  
Environmental Dna ◽  
Magnetic Bead ◽  
Extraction Methods ◽  
Environmental Research ◽  
Rrna Gene

Abstract The characterization of microbial community dynamics using genomic methods is rapidly expanding, impacting many fields including medical, ecological, and environmental research and applications. One of the biggest challenges for such studies is the isolation of environmental DNA (eDNA) from a variety of samples, diverse microbes, and widely variable community compositions. The current study developed environmentally friendly, user safe, economical, and high throughput eDNA extraction methods for mixed aquatic microbial communities and tested them using 16 s rRNA gene meta-barcoding. Five different lysis buffers including (1) cetyltrimethylammonium bromide (CTAB), (2) digestion buffer (DB), (3) guanidinium isothiocyanate (GITC), (4) sucrose lysis (SL), and (5) SL-CTAB, coupled with four different purification methods: (1) phenol-chloroform-isoamyl alcohol (PCI), (2) magnetic Bead-Robotic, (3) magnetic Bead-Manual, and (4) membrane-filtration were tested for their efficacy in extracting eDNA from recreational freshwater samples. Results indicated that the CTAB-PCI and SL-Bead-Robotic methods yielded the highest genomic eDNA concentrations and succeeded in detecting the core microbial community including the rare microbes. However, our study recommends the SL-Bead-Robotic eDNA extraction protocol because this method is safe, environmentally friendly, rapid, high-throughput and inexpensive.

scholarly journals Supplementary material to "Characterization of NR-PM<sub>1</sub> and source apportionment of organic aerosol in Krakow, Poland"

2021 ◽  
Author(s):  
Anna K. Tobler ◽  
Alicja Skiba ◽  
Francesco Canonaco ◽  
Griša Močnik ◽  
Pragati Rai ◽  
...  
Keyword(s):  
Source Apportionment ◽  
Organic Aerosol ◽  
Supplementary Material

scholarly journals Supplementary material to "Characterization of the Particle Emission from Ships Operating at Sea Using Unmanned Aerial Vehicles"

2017 ◽  
Author(s):  
Tommaso F. Villa ◽  
Reece Brown ◽  
E. Rohan Jayaratne ◽  
L. Felipe Gonzalez ◽  
Lidia Morawska ◽  
...  
Keyword(s):  
Unmanned Aerial Vehicles ◽  
Particle Emission ◽  
Aerial Vehicles ◽  
Supplementary Material

scholarly journals Supplementary material to "Performance Characterization of Low-cost Air Quality Sensors for Off-grid Deployment in Rural Malawi"

2021 ◽  
Author(s):  
Ashley S. Bittner ◽  
Eben S. Cross ◽  
David H. Hagan ◽  
Carl Malings ◽  
Eric Lipsky ◽  
...  
Keyword(s):  
Air Quality ◽  
Low Cost ◽  
Performance Characterization ◽  
Supplementary Material

scholarly journals Metagenomic Profiling: Microarray Analysis of an Environmental Genomic Library

2003 ◽  
Vol 69 (8) ◽  
pp. 4927-4934 ◽  
Author(s):  
Jonathan L. Sebat ◽  
Frederick S. Colwell ◽  
Ronald L. Crawford
Keyword(s):  
Genomic Dna ◽  
Genomic Library ◽  
Hydrogen Oxidation ◽  
Metagenomic Library ◽  
Environmental Dna ◽  
Cosmid Library ◽  
Genomic Libraries ◽  
Novel Approach ◽  
Ecological Importance

ABSTRACT Genomic libraries derived from environmental DNA (metagenomic libraries) are useful for characterizing uncultured microorganisms. However, conventional library-screening techniques permit characterization of relatively few environmental clones. Here we describe a novel approach for characterization of a metagenomic library by hybridizing the library with DNA from a set of groundwater isolates, reference strains, and communities. A cosmid library derived from a microcosm of groundwater microorganisms was used to construct a microarray (COSMO) containing ∼1-kb PCR products amplified from the inserts of 672 cosmids plus a set of 16S ribosomal DNA controls. COSMO was hybridized with Cy5-labeled genomic DNA from each bacterial strain, and the results were compared with the results for a common Cy3-labeled reference DNA sample consisting of a composite of genomic DNA from multiple species. The accuracy of the results was confirmed by the preferential hybridization of each strain to its corresponding rDNA probe. Cosmid clones were identified that hybridized specifically to each of 10 microcosm isolates, and other clones produced positive results with multiple related species, which is indicative of conserved genes. Many clones did not hybridize to any microcosm isolate; however, some of these clones hybridized to community genomic DNA, suggesting that they were derived from microbes that we failed to isolate in pure culture. Based on identification of genes by end sequencing of 17 such clones, DNA could be assigned to functions that have potential ecological importance, including hydrogen oxidation, nitrate reduction, and transposition. Metagenomic profiling offers an effective approach for rapidly characterizing many clones and identifying the clones corresponding to unidentified species of microorganisms.

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