scholarly journals Microscopic and spectroscopic investigations of uranium(VI) reduction by <i>Desulfosporosinus hippei</i> DSM 8344

2021 ◽  
Vol 1 ◽  
pp. 155-156
Author(s):  
Stephan Hilpmann ◽  
Robin Steudtner ◽  
Björn Drobot ◽  
René Hübner ◽  
Frank Bok ◽  
...  
Keyword(s):  
Radioactive Waste ◽  
Reduction Process ◽  
Opalinus Clay ◽  
Disposal Site ◽  
Luminescence Spectroscopy ◽  
Worst Case ◽  
High Level Radioactive Waste ◽  
Sulfate Reducing ◽  
High Level

Abstract. Clay formations are potential host rocks for the long-term storage of high-level radioactive waste in a deep geological repository. Bentonites are supposed to serve as backfill material, not only for a final disposal site in clay formations but also in crystalline rock. For a long-term safety assessment, various aspects must be taken into account. Besides geological, geochemical and geophysical considerations, naturally occurring microorganisms also play a crucial part in the environment of such a repository. In the event of a worst-case scenario when water enters the disposal site, they can interact with the radionuclides and change for example the chemical speciation or the oxidation state (Lloyd et al., 2002). Desulfosporosinus spp. are an important representative of anaerobic, sulfate-reducing microorganisms, which are present in clay formations as well as in bentonites. Various studies have shown that they play a major role in the microbial communities of these surroundings (Bagnoud et al., 2016; Matschiavelli et al., 2019). A closely related microorganism to the isolated species is Desulfosporosinus hippei DSM 8344, which was originally found in permafrost soil (Vatsurina et al., 2008). This bacterium was used to investigate its interactions with uranium(VI) especially regarding the reduction to the less mobile uranium(IV). Time-dependent reduction experiments in artificial Opalinus Clay pore water (Wersin et al., 2011) (100 µM uranium(VI), pH 5.5) showed the removal of about 80 % of the uranium(VI) from the supernatants within 48 h. Corresponding UV/Vis measurements of the dissolved cell pellets exhibited an increasing proportion of uranium(IV) in the cell-bound uranium. Calculations with the inclusion of extinction coefficients led to a ratio of 39 % uranium(IV) after 1 week. Therefore, a combined sorption-reduction process is a possible interaction mechanism. Time-resolved laser-induced luminescence spectroscopy verified the presence of two uranium(VI) species in the supernatant. A comparison with reference spectra led to an assignment to a uranyl(VI) lactate and a uranyl(VI) carbonate complex. The species distribution showed a decrease of the proportion of the lactate species with time, whereas the proportion of the carbonate species remained almost constant. Uranium aggregates are formed on the cell surface during the process, as determined by transmission electron microscopy (TEM). Furthermore, uranium occurs inside and outside the cells as well as vesicles containing uranium. These findings help to close existing gaps in a comprehensive safeguard concept for a repository for high-level radioactive waste in clay rock. Moreover, this study provides new insights into the interactions of sulfate-reducing microorganisms with uranium(VI).

scholarly journals Microscopic and spectroscopic bioassociation study of uranium(VI) with an archaeal Halobacterium isolate

PLoS ONE ◽  
2022 ◽  
Vol 17 (1) ◽  
pp. e0262275
Author(s):  
Stephan Hilpmann ◽  
Miriam Bader ◽  
Robin Steudtner ◽  
Katharina Müller ◽  
Thorsten Stumpf ◽  
...  
Keyword(s):  
Radioactive Waste ◽  
Rock Salt ◽  
Luminescence Spectroscopy ◽  
Spectroscopic Techniques ◽  
High Level Radioactive Waste ◽  
Long Term Storage ◽  
High Level ◽  
The Impact ◽  
Term Storage

The safe disposal of high-level radioactive waste in a deep geological repository is a huge social and technical challenge. So far, one of the less considered factors needed for a long-term risk assessment, is the impact of microorganisms occurring in the different host rocks. Even under the harsh conditions of salt formations different bacterial and archaeal species were found, e. g. Halobacterium sp. GP5 1–1, which has been isolated from a German rock salt sample. The interactions of this archaeon with uranium(VI), one of the radionuclides of major concern for the long-term storage of high-level radioactive waste, were investigated. Different spectroscopic techniques, as well as microscopy, were used to examine the occurring mechanisms on a molecular level leading to a more profound process understanding. Batch experiments with different uranium(VI) concentrations showed that the interaction is not only a simple, but a more complex combination of different processes. With the help of in situ attenuated total reflection Fourier-transform infrared spectroscopy the association of uranium(VI) onto carboxylate groups was verified. In addition, time-resolved laser-induced luminescence spectroscopy revealed the formation of phosphate and carboxylate species within the cell pellets as a function of the uranium(VI) concentration and incubation time. The association behavior differs from another very closely related halophilic archaeon, especially with regard to uranium(VI) concentrations. This clearly demonstrates the importance of studying the interactions of different, at first sight very similar, microorganisms with uranium(VI). This work provides new insights into the microbe-uranium(VI) interactions at highly saline conditions relevant to the long-term storage of radioactive waste in rock salt.

Significance of long-term climate evolution and associated impacts on the long-term safety of a high-level radioactive waste repository within the German siting process

2021 ◽  
Author(s):  
Marc Wengler ◽  
Astrid Göbel ◽  
Eva-Maria Hoyer ◽  
Axel Liebscher ◽  
Sönke Reiche ◽  
...  
Keyword(s):  
Radioactive Waste ◽  
Site Selection ◽  
Phase 1 ◽  
High Level Radioactive Waste ◽  
Safety Requirements ◽  
Climate Evolution ◽  
Safety Assessments ◽  
High Level ◽  
Host Rocks

&lt;p&gt;According to the 'Act on the Organizational Restructuring in the Field of Radioactive Waste Disposal' the BGE was established in 2016. The amended 'Repository Site Selection Act' (StandAG) came into force in July 2017 and forms the base for the site selection by clearly defining the procedure. According to the StandAG the BGE implements the participative, science-based, transparent, self-questioning and learning procedure with the overarching aim to identify the site for a high-level radioactive waste (HLW) repository in a deep geological formation with best possible safety conditions for a period of one million years.&lt;/p&gt;&lt;p&gt;The German site selection procedure consists of three phases, of which Phase 1 is divided into two steps. Starting with a blanc map of Germany, the BGE completed Step 1 in September 2020 and identified 90 individual sub-areas that provide favorable geological conditions for the safe disposal of HLW in the legally considered host rocks; rock salt, clay and crystalline rock. Based on the results of Step 1, the on-going Step 2 will narrow down these sub-areas to siting regions for surface exploration within Phase 2 (&amp;#167; 14 StandAG). Central to the siting process are representative (Phase 1), evolved (Phase 2) and comprehensive (Phase 3) preliminary safety assessments according to &amp;#167; 27 StandAG.&lt;/p&gt;&lt;p&gt;The ordinances on 'Safety Requirements' and 'Preliminary Safety Assessments' for the disposal of high-level radioactive waste from October 2020 regulate the implementation of the preliminary safety assessments within the different phases of the siting process. Section 2 of the 'Safety Requirements' ordinance provides requirements to evaluate the long-term safety of the repository system; amongst others, it states that all potential effects that may affect the long-term safety of the repository system need to be systematically identified, described and evaluated as &amp;#8220;expected&amp;#8221; or &amp;#8220;divergent&amp;#8221; evolutions. Additionally, the ordinance on 'Preliminary Safety Assessments' states in &amp;#167; 7, amongst others, that the geoscientific long-term prediction is a tool to identify and to evaluate geogenic processes and to infer &amp;#8220;expected&amp;#8221; and &amp;#8220;divergent&amp;#8221; evolutions from those. Hence, considering the time period of one million years for the safe disposal of the HLW and the legal requirements, it is essential to include long-term climate evolution in the German site selection process to evaluate the impact of various climate-related scenarios on the safety of the whole repository system.&lt;/p&gt;&lt;p&gt;To better understand and evaluate the influence of climate-related processes on the long-term safety of a HLW repository, climate-related research will be a part of the BGE research agenda. Potential research needs may address i) processes occurring on glacial &amp;#8211; interglacial timescales (e.g. the inception of the next glaciation, formation and depth of permafrost, glacial troughs, sub-glacial channels, sea-level rise, orbital forcing) and their future evolutions, ii) effects on the host rocks and the barrier system(s) as well as iii) the uncertainties related to these effects but also to general climate models and predictions.&lt;/p&gt;

NUMO’s Open Solicitation of Volunteer Municipalities for a Potential Disposal Site

2003 ◽  
Author(s):  
Kazumi Kitayama
Keyword(s):  
Radioactive Waste ◽  
Preliminary Investigation ◽  
Disposal Site ◽  
Nuclear Waste Management ◽  
The Public ◽  
Construction Operation ◽  
High Level ◽  
Year 2000 ◽  
Selection Of

In the year 2000, the Japanese geological disposal program for high-level radioactive waste (HLW) moved from the phase of generic research and development into the phase of implementation. Following legislation entitled the “Specified Radioactive Waste Final Disposal Act” (hereafter “the Act”), the Nuclear Waste Management Organization of Japan (NUMO) was established as the implementing organization in October 2000. The assigned activities of NUMO include repository site selection, developing relevant license applications and construction, operation and closure of the repository. To initiate the first stage, NUMO has chosen an “open solicitation” approach for finding candidate sites in the belief that the support of local communities is essential to the success of this highly public, long-term project extending over more than a century. Based on this concept, NUMO announced the start of open solication for volunteer municipalities for selection of Preliminary Investigation Areas to the public on December 19, 2002. This paper describes NUMO’s open solicitation of volunteer municipalities for a potential disposal site.

The role of microorganisms in the bentonite barrier of high-level radioactive waste repositories

2020 ◽  
Author(s):  
Nicole Matschiavelli ◽  
Magdalena Dressler ◽  
Tom Neubert ◽  
Sindy Kluge ◽  
Ariette Schierz ◽  
...  
Keyword(s):  
Radioactive Waste ◽  
Thermophilic Bacteria ◽  
Disposal Site ◽  
Dissolution Behavior ◽  
Strictly Anaerobic ◽  
Dependent Manner ◽  
Metabolic Potential ◽  
High Level Radioactive Waste ◽  
Geochemical Parameters ◽  
High Level

&lt;p&gt;The global production of 12,000 metric tonnes of high-level radioactive waste (HLW) every year is a big challenge with respect to its safe long-term storage. In the favored multi-barrier system, bentonite is discussed as a geo-technical barrier in many disposal programs worldwide. The bentonite seals the space between the canister containing the HLW and the surrounding host rock, thereby fulfilling two major tasks: 1) slow down the process of corrosion when water enters the disposal site, and 2) hinder the discharge of radionuclides into the bio-geosphere in case of a leaking canister. Due to their metabolic activity, microorganisms could significantly influence the properties of the bentonite barrier. In order to investigate the metabolic potential of naturally occurring microorganisms, we conducted anaerobic bentonite-slurry experiments containing uncompacted bentonite and a synthetic Opalinus Clay pore water solution. Within one-year incubation at 30 and 60&amp;#160;&amp;#176;C, lactate- or H&lt;sub&gt;2&lt;/sub&gt;-stimulated microcosms at 30 &amp;#176;C showed the dominance and activity of strictly anaerobic, sulfate-reducing and spore-forming microorganisms. Consequently, hydrogen sulfide gas was generated in the respective set ups, leading to the formation of fractures and iron-sulfur precipitations. Experiments that incubated at 60 &amp;#176;C, showed the dominance of thermophilic bacteria, independent of the presence of substrates. The respective set-ups showed/revealed no significant changes in the analyzed bio-geochemical parameters. The obtained results clearly show that indigenous microorganisms evolve in a temperature- and substrate-dependent manner. The formed metabolites can potentially affect the dissolution behavior of minerals and ions within the bentonite as well as corrosion processes and require further investigations.&lt;/p&gt;

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