Multiple Target Localisation at over 100 FPS

2009 ◽  
Author(s):  
Simon Taylor ◽  
Tom Drummond
Keyword(s):  
Multiple Target ◽  
Target Localisation

Signal transduction therapy of colon tumors with multiple target kinase inhibitors

2009 ◽  
Vol 47 (05) ◽  
Author(s):  
G Kéri ◽  
L Őrfi ◽  
Z Greff ◽  
Z Varga ◽  
B Szokol ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Kinase Inhibitors ◽  
Multiple Target ◽  
Colon Tumors ◽  
Signal Transduction Therapy

Research on multiple-target tracking of heliborne radar

2009 ◽  
Author(s):  
Chang Jun ◽  
Zhang Lin-yi ◽  
Yang Yong
Keyword(s):  
Target Tracking ◽  
Multiple Target Tracking ◽  
Multiple Target

scholarly journals Multiple target data-driven models to enable sustainable process manufacturing: An industrial bioprocess case study

2021 ◽  
Vol 296 ◽  
pp. 126242
Author(s):  
Oliver J. Fisher ◽  
Nicholas J. Watson ◽  
Laura Porcu ◽  
Darren Bacon ◽  
Martin Rigley ◽  
...  
Keyword(s):  
Data Driven ◽  
Multiple Target ◽  
Process Manufacturing ◽  
Target Data

scholarly journals Split NanoLuc technology allows quantitation of interactions between PII protein and its receptors with unprecedented sensitivity and reveals transient interactions

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Rokhsareh Rozbeh ◽  
Karl Forchhammer
Keyword(s):  
The Novel ◽  
Multiple Target ◽  
Interacting Protein ◽  
Effector Molecule ◽  
Target Proteins ◽  
Pii Protein ◽  
Protein X ◽  
Cellular Context ◽  
Pii Proteins ◽  
Transient Interactions

AbstractPII proteins constitute a widespread signal transduction superfamily in the prokaryotic world. The canonical PII signal proteins sense metabolic state of the cells by binding the metabolite molecules ATP, ADP and 2-oxoglutarate. Depending on bound effector molecule, PII proteins interact with and modulate the activity of multiple target proteins. To investigate the complexity of interactions of PII with target proteins, analytical methods that do not disrupt the native cellular context are required. To this purpose, split luciferase proteins have been used to develop a novel complementation reporter called NanoLuc Binary Technology (NanoBiT). The luciferase NanoLuc is divided in two subunits: a 18 kDa polypeptide termed “Large BiT” and a 1.3 kDa peptide termed “Small BiT”, which only weakly associate. When fused to proteins of interest, they reconstitute an active luciferase when the proteins of interest interact. Therefore, we set out to develop a new NanoBiT sensor based on the interaction of PII protein from Synechocystis sp. PCC6803 with PII-interacting protein X (PipX) and N-acetyl-L-glutamate kinase (NAGK). The novel NanoBiT sensor showed unprecedented sensitivity, which made it possible to detect even weak and transient interactions between PII variants and their interacting partners, thereby shedding new light in PII signalling processes.

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