scholarly journals Genetic diversity assessment of aegilops germplasm by RAPD molecular markers

2011 ◽  
Vol 2 (2) ◽  
pp. 197-202 ◽  
Author(s):  
Amin Baghizadeh ◽  
Sahar Khosravi
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Diversity Assessment

scholarly journals Genetic diversity assessment of sesame core collection in China by phenotype and molecular markers and extraction of a mini-core collection

BMC Genetics ◽  
2012 ◽  
Vol 13 (1) ◽  
pp. 102 ◽  
Author(s):  
Yanxin Zhang ◽  
Xiurong Zhang ◽  
Zhuo Che ◽  
Linhai Wang ◽  
Wenliang Wei ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Core Collection ◽  
Mini Core Collection ◽  
Diversity Assessment

scholarly journals Genetic Diversity Assessment of Luffa aegyptiaca Landraces Endemic in Egypt Based on Some Molecular Markers

2016 ◽  
Vol 26 (2) ◽  
pp. 209-217
Author(s):  
Mohammad Abdel Sttar Al Tahlawy ◽  
Mahmoud Abdel Aziz Ibrahim ◽  
Mohamed Ahmed Matter ◽  
Mervat El Sayed Mohamed ◽  
Mahmoud Mohamed Sakr
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Total Proteins ◽  
Pcr Marker ◽  
Polymorphic Loci ◽  
Average Percentage ◽  
Rapd Pcr ◽  
Diversity Assessment ◽  
Sds Page ◽  
Luffa Aegyptiaca

Luffa aegyptiaca is a popular climbing herb endemic in Egypt. We studied the genetic diversity among ten Luffa landraces (Cairo, Beni Suef, Menoufiya, Damietta, Banha, Aswan, Kafr el?Sheikh, Bir el?Abd, MarsaMatruh and Asyut) collected from different districts in Egypt. The results obtained from DNA fingerprinting revealed that there were polymorphic loci with average percentage of 44.6 among collected landraces whereas polymorphic loci obtained from SDS?PAGE were 23%. Discrimination between landraces was more efficient by using RAPD?PCR marker than total proteins SDS?PAGE which showed a limited level of intraspecific diversity.Plant Tissue Cult. & Biotech. 26(2): 209-217, 2016 (December)

scholarly journals Genetic Diversity Assessment of Lines and Varieties in Winter Rapeseed (Brassica napus L.) using RAPD and SSR Molecular Markers

2016 ◽  
Vol 8 (17) ◽  
pp. 139-131 ◽  
Author(s):  
حسن هدایتی مرزونی ◽  
حبیب اله سمیع زاده لاهیجی ◽  
◽  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Brassica Napus ◽  
Brassica Napus L ◽  
Diversity Assessment ◽  
Winter Rapeseed

Genetic diversity assessment of summer squash landraces using molecular markers

2013 ◽  
Vol 40 (7) ◽  
pp. 4269-4274 ◽  
Author(s):  
Emad A. Mady ◽  
Alaa Al-Din Helaly ◽  
Abdel Naem Abu El-Hamd ◽  
Arafa Abdou ◽  
Shamel A. Shanan ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Diversity Assessment ◽  
Summer Squash

Genetic Diversity Assessment of Upland Cotton Variety Resources in China Based on Phenotype Traits and Molecular Markers

Crop Science ◽  
2016 ◽  
Vol 57 (1) ◽  
pp. 290-301 ◽  
Author(s):  
Cheng-qi Li ◽  
Li Song ◽  
Ya-juan Zhu ◽  
Yong-jie Zhai ◽  
Qing-lian Wang
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Upland Cotton ◽  
Cotton Variety ◽  
Diversity Assessment

scholarly journals Genetic diversity and population structure of Vernonia [Vernonia galamensis (Cass.) Less] populations from Ethiopia revealed by SSR markers

2020 ◽  
Author(s):  
Alemneh Mideksa Egu ◽  
Kassahun Tesfaye ◽  
Kifle Dagne New ◽  
Xuebo Hu New
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Ssr Markers ◽  
Polymorphic Information Content ◽  
In Situ Conservation ◽  
Germplasm Conservation ◽  
Pharmaceutical Products ◽  
Gene Pools ◽  
Vernonia Galamensis ◽  
Diversity Assessment

Abstract Background: Vernonia (Vernonia galamensis) is a potential novel industrial crop due to high demand for its natural epoxidized oil, which can be used for the manufacturing of oleochemicals such as paints, plastic formulations (polyvinyl chloride), and pharmaceutical products. This study was initiated for the systematic and intensive genetic diversity assessment of V. galamensis accessions by SSR molecular markers to minimize the existing research gaps, provide a clue for germplasm conservation and further research. Results: Twenty SSR markers were used for genetic diversity analyses of 150 individual V. galamensis accessions representing 10 populations, from which a total of 79 bands were identified for the 20 loci. All the loci used showed high polymorphism that ranged from 0.50 to 0.96, while the mean observed heterozygosity (Ho) was 0.15 across all the 20 markers evaluated. The molecular variance analysis (AMOVA) showed significant variations among populations which accounted for 11% of the variations. Populations clustering showed that the dendrogram and principal coordinate’s analysis roughly classified the accessions into four groups. However, the Bayesian model-based clustering (STRUCTURE) grouped into 6 (K = 6) major gene pools. Since, the cluster and the STRUCTURE analyses did not group the populations into sharply distinct clusters, due to presence of gene flow and mode of reproduction of the plant. Conclusions: The SSR molecular markers used in this study are highly polymorphic. Among the ten populations, East Showa and East Hararghe revealed higher genetic diversity, signaled that these areas are the hotspots for in-situ conservation of V. galamensis. In addition, the values of SSR markers such as heterozygosity, Shannon‘s index, polymorphic information content, and population clusters are important baseline information for future V. galamensis cultivation, breeding and genetic resource conservation endeavors in Ethiopia.

scholarly journals Genetic diversity assessment of ancient mulberry (Morus spp.) in Lebanon using morphological, chemical and molecular markers (SSR and ISSR)

2021 ◽  
Vol 35 (3) ◽  
pp. 243-253
Author(s):  
Aline Kadri ◽  
Shoaib Saleh ◽  
Ahmad Elbitar ◽  
Ali Chehade
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Crop Improvement ◽  
Germplasm Management ◽  
Diversity Assessment ◽  
Geographical Regions ◽  
Relative Separation ◽  
Morus Spp ◽  
High Level ◽  
Issr Primers

Lebanon has ancient mulberry trees which are the remnants of the abundant orchards that dominated its lands during the nineteenth century. Lebanese mulberry germplasm has not been assessed yet. This study aims to collect local old rainfed mulberry accessions from different geographical regions and assess their diversity by using morphological and molecular markers (SSR and ISSR). Genetic diversity of 70 accessions of mulberry were evaluated by using 27 morphological traits. The dendrogram based on the morphological attributes showed a relative separation of the different accessions based on fruits color and taste. Molecular analysis was performed for the accessions by using selected SSR and ISSR primers. The primers marked a high discriminating power (0.7 to 0.89). The dendrogram constructed on the base of UPGMA method showed 13 different groups. The clustering patterns indicated no location nor local name specificity among mulberry accessions. The combination of SSR and ISSR primers was informative for estimating the extent of mulberry genetic diversity. It can be concluded that there is a high level of genetic diversity within mulberry trees in Lebanon. These results will be useful for mulberry germplasm management in terms of biodiversity protection and as a valuable source of gene pool for crop improvement.

scholarly journals Molecular genetic diversity assessment of Citrus species grown in Iran revealed by SSR, ISSR and CAPS molecular markers

2017 ◽  
Vol 2 (8) ◽  
pp. 22
Author(s):  
Ata Allah Sharafi ◽  
Asad Asadi Abkenar ◽  
Ali Sharafi
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Molecular Genetic ◽  
Citrus Species ◽  
Molecular Genetic Diversity ◽  
Diversity Assessment

En este estudio, se analizó la diversidad genética en 19 cultivares de cítricos mediante la repetición de secuencia simple (SSR), la repetición de secuencia inter-simple (ISSR) y los marcadores de secuencia polimórfica amplificada segmentada (CAPS). Se utilizaron nueve cebadores para SSR, nueve cebadores ISSR y dos cebadores para CAPS para la puntuación del alelo. Se analizaron una región de ADN de cloroplasto (rbcL-ORF106) y una región de ADN mitocondrial (18S-5S) usando marcador de secuencia polimórfica amplificada escindida (CAPS) en 19 accesiones de cítricos cultivadas en Irán. En total, se detectaron 45 genes SSR y 131 ISSR polimórficos alelos y organelos del genoma del árbol. El análisis de conglomerados de los datos SSR y ISSR se realizó utilizando el método UPGMA y se basó en el coeficiente de Jaccard. El resultado de esta investigación mostró que los cebadores SSR e ISSR eran altamente informativos y eficientes para detectar la variabilidad genética y las relaciones de las accesiones de los cítricos. Y el análisis de marcadores de CAPS Los resultados mostraron que Bakraee y uno de tipo off cal mexicana tenían un patrón de bandas similar al Clementine Mandarin, mientras que Pummelo se consideraba como padre materno de otros genotipos estudiados Citron considerado como padre padre mostró un patrón de bandas definido entre 19 genotipos estudiados que confirmó Citoplasma herencia de orgánulos celulares de la madre.  

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