Genetic Diversity Assessment in Chickpea Using Morphological Characters and Start Codon Targeted (SCoT) Molecular Markers

To evaluate the genetic diversity among 10 cultivars of anthurium were performed using three molecular markers such as Start Codon Targeted (SCoT) and Conserved DNA-derived Polymorphism (CDDP), and Random Amplification of Polymorphic DNA (RAPD). Polymorphism index content (PIC) was calculated 0.39, 0.42 and 0.37 for RAPD, SCoT and CDDP, respectively. This result showed all the three molecular markers had almost an identical potential in estimating genetic diversity. Cluster analysis using SCoT, CDDP and RAPD divided the cultivars to three distinct clusters. The similarity matrix obtained through SCoT and CDDP was positively significantly correlated (r = 0.76, p < 0.01). This is the first report in which the efficiency of two targeted DNA region molecular markers (SCoT and CDDP) together with RAPD technique have been compared with each other in a set of anthurium cultivras. Results suggested that SCOT, CDDP and RAPD fingerprinting techniques are of sufficient ability to detect polymorphism in anthurium cultivars. Plant Tissue Cult. & Biotech. 28(2): 171-182, 2018 (December)

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Genetic diversity assessment of sesame core collection in China by phenotype and molecular markers and extraction of a mini-core collection

BMC Genetics ◽

10.1186/1471-2156-13-102 ◽

2012 ◽

Vol 13 (1) ◽

pp. 102 ◽

Cited By ~ 25

Author(s):

Yanxin Zhang ◽

Xiurong Zhang ◽

Zhuo Che ◽

Linhai Wang ◽

Wenliang Wei ◽

...

Keyword(s):

Genetic Diversity ◽

Molecular Markers ◽

Core Collection ◽

Mini Core Collection ◽

Diversity Assessment

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Analysis of genetic diversity in Prunus sibirica L. in inner Mongolia using SCoT molecular markers

Genetic Resources and Crop Evolution ◽

10.1007/s10722-021-01284-4 ◽

2022 ◽

Author(s):

Ha Buer ◽

Sa Rula ◽

Zi Yuan Wang ◽

Shu Fang ◽

Yu´e Bai

Keyword(s):

Genetic Diversity ◽

Molecular Markers ◽

Genetic Differentiation ◽

Inner Mongolia ◽

Start Codon ◽

Group Method ◽

Pair Group ◽

Protection And Utilization ◽

Minimum Number ◽

Polymorphic Bands

AbstractPopulation genetic diversity contributes to the protection and utilization of germplasm resources, especially via genetic breeding. In the present study, start codon targeted polymorphism (SCoT) molecular markers were used to study the genetic diversity of 278 individuals from 10 Prunus sibirica L. populations in Inner Mongolia. A total of 289 polymorphic bands were amplified with 23 SCoT primers, showing a polymorphism percentage of 98.87% and an average of 12.6 polymorphic bands per primer. The SCoT21, SCoT32, and SCoT53 primers amplified up to 17 bands, and the polymorphism percentage was 100%. The minimum number of bands amplified by SCoT25 was 9, and the polymorphism percentage was 90%. Therefore, SCoT molecular markers were shown to be highly polymorphic and suitable for genetic diversity studies of P. sibirica in Inner Mongolia. The analysis of molecular variance showed that 39% of the observed genetic differentiation occurred among populations and 61% occurred within populations, indicating that the genetic differentiation within populations was greater than that among populations. The results of the unweighted pair-group method with an arithmetic cluster analysis, principal coordinate analysis and STRUCTURE analysis were basically the same and divided the 278 individuals from the 10 populations into 2 groups. The results indicated that the efficient SCoT molecular marker-based genetic diversity analysis of P. sibirica in Inner Mongolia can provide a reference for P. sibirica variety breeding and resource development.

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Start codon targeted (SCoT) polymorphism reveals genetic diversity of Manilkara in Thailand

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d210232 ◽

2020 ◽

Vol 21 (2) ◽

Author(s):

Ongkarn Vanijajiva

Keyword(s):

Genetic Diversity ◽

Genetic Resources ◽

Genetic Improvement ◽

Morphological Characters ◽

Start Codon ◽

Similarity Coefficients ◽

Scot Marker ◽

Marker Analysis ◽

Dna Profiles ◽

Relationship Of

Abstract. Vanijajiva O. 2020. Start codon targeted (SCoT) polymorphism reveals genetic diversity of Manilkara in Thailand. Biodiversitas 21: 666-673. Manilkara consists of four species, namely, Manilkara hexandra, M. kauki, M. littoralis, and M. zapota in Thailand. Particularly, M. zapota (sapodilla), commonly known as Lamut in Thailand, is one of commercially notable edible fruits continues to be a highly valued tree and the fruits are a source of food and income. The objective of this research was to determine the diversity and relationship of Manilkara accessions derived from districts in Thailand based on DNA profiles. Genetic diversity and relationship of all accessions were investigated using Start Codon Targeted (SCoT) marker analysis. Of 30 SCoT primers screened, 27 SCoT primers were identified to be polymorphism. A total of 176 DNA bands with size varied from 120 bp to 1200 bp were amplified, and 101 (57.38%) of them were polymorphic. Based on similarity coefficients, total of 36 accessions were clustered into two groups. The cluster of polymorphism generated by SCoT was associated with their locality and morphological characters. Therefore, the present data provide high-valued information for the management of germplasm, genetic improvement, and conservation of the genetic resources of Manilkara accessions.

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Genetic diversity in Jatropha species from different regions of Brazil based on morphological characters and inters-simple sequence repeat (ISSR) molecular markers

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb2014.14368 ◽

2015 ◽

Vol 14 (25) ◽

pp. 2066-2079 ◽

Cited By ~ 3

Author(s):

Silva Rosa Pazeto Mariana ◽

Helena Uneda Trevisoli Sandra ◽

Arcenio Pimentel Correa Aretha ◽

Formice Vianna Viviane ◽

Carvalho Leite Daniel ◽

...

Keyword(s):

Genetic Diversity ◽

Molecular Markers ◽

Simple Sequence Repeat ◽

Morphological Characters ◽

Sequence Repeat ◽

Issr Molecular Markers ◽

Simple Sequence

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Genetic Diversity Assessment of Luffa aegyptiaca Landraces Endemic in Egypt Based on Some Molecular Markers

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v26i2.30571 ◽

2016 ◽

Vol 26 (2) ◽

pp. 209-217

Author(s):

Mohammad Abdel Sttar Al Tahlawy ◽

Mahmoud Abdel Aziz Ibrahim ◽

Mohamed Ahmed Matter ◽

Mervat El Sayed Mohamed ◽

Mahmoud Mohamed Sakr

Keyword(s):

Genetic Diversity ◽

Molecular Markers ◽

Total Proteins ◽

Pcr Marker ◽

Polymorphic Loci ◽

Average Percentage ◽

Rapd Pcr ◽

Diversity Assessment ◽

Sds Page ◽

Luffa Aegyptiaca

Luffa aegyptiaca is a popular climbing herb endemic in Egypt. We studied the genetic diversity among ten Luffa landraces (Cairo, Beni Suef, Menoufiya, Damietta, Banha, Aswan, Kafr el?Sheikh, Bir el?Abd, MarsaMatruh and Asyut) collected from different districts in Egypt. The results obtained from DNA fingerprinting revealed that there were polymorphic loci with average percentage of 44.6 among collected landraces whereas polymorphic loci obtained from SDS?PAGE were 23%. Discrimination between landraces was more efficient by using RAPD?PCR marker than total proteins SDS?PAGE which showed a limited level of intraspecific diversity.Plant Tissue Cult. & Biotech. 26(2): 209-217, 2016 (December)

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