Genetic Diversity Assessment of Luffa aegyptiaca Landraces Endemic in Egypt Based on Some Molecular Markers

Luffa aegyptiaca is a popular climbing herb endemic in Egypt. We studied the genetic diversity among ten Luffa landraces (Cairo, Beni Suef, Menoufiya, Damietta, Banha, Aswan, Kafr el?Sheikh, Bir el?Abd, MarsaMatruh and Asyut) collected from different districts in Egypt. The results obtained from DNA fingerprinting revealed that there were polymorphic loci with average percentage of 44.6 among collected landraces whereas polymorphic loci obtained from SDS?PAGE were 23%. Discrimination between landraces was more efficient by using RAPD?PCR marker than total proteins SDS?PAGE which showed a limited level of intraspecific diversity.Plant Tissue Cult. & Biotech. 26(2): 209-217, 2016 (December)

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Genetic diversity assessment of sesame core collection in China by phenotype and molecular markers and extraction of a mini-core collection

BMC Genetics ◽

10.1186/1471-2156-13-102 ◽

2012 ◽

Vol 13 (1) ◽

pp. 102 ◽

Cited By ~ 25

Author(s):

Yanxin Zhang ◽

Xiurong Zhang ◽

Zhuo Che ◽

Linhai Wang ◽

Wenliang Wei ◽

...

Keyword(s):

Genetic Diversity ◽

Molecular Markers ◽

Core Collection ◽

Mini Core Collection ◽

Diversity Assessment

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Genetic diversity of the Colombian Creole donkey in the Department of Sucre

Veterinarska stanica ◽

10.46419/vs.51.6.2 ◽

2020 ◽

Vol 51 (6) ◽

pp. 611-619

Author(s):

Adrian Medina-Montes ◽

Darwin Hernández-Herrera ◽

Javier Beltrán-Herrera ◽

Donicer Montes-Vergara

Keyword(s):

Genetic Diversity ◽

Population Structure ◽

Molecular Markers ◽

Structure Analysis ◽

Genetic Distances ◽

Entire Population ◽

Polymorphic Loci ◽

Population Structure Analysis

The objective of this study was to evaluate the genetic diversity of the Colombian Creole donkey in the Department of Sucre using Random Amplified Microsatellites (RAM) molecular markers. In 100 individuals from the five subregions of the department, DNA was extracted and five RAM primers were amplified by PCR. In all, 291 bands were found, on average 11.96±1.45 per primer, the highest value in CCA (18±2.23) and the lowest in TG and GT (8.8±0.44). CA was the most polymorphic primer (88.09±10.91%) with the highest heterozygosity value (He) (0.376±0.021), while the lowest was GT (0.341±0.076 and 0.101±0.040, respectively). Intrapopulation analysis showed an average of 66.50±1.72 bands, of which 89.86±24.04% were polymorphic. The highest number of bands (63±3.84) was found in the Gulf of Morrosquillo (GO) subpopulation, and the lowest in Mojana (MO) (48±2.88); however, the highest value of polymorphic loci (81.16%) and He (0.335±0.022) were found in the Montes de María (MM) subpopulation, making it the most diverse. The average genetic diversity for the entire population was 0.351±0.021 bands. The population structure analysis showed a 10% variation between subpopulations, with an FST value of 0.17±0.01 (P<0.05). Genetic distances between subpopulations showed that MO and GO were the most distant. The RAM markers are effective in assessing the genetic diversity of the Creole donkey, which has high values of genetic diversity, particularly the MM subpopulation. The genetic revealed structure could be the result of natural geographical barriers between the subregions.

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Genetic diversity assessment of aegilops germplasm by RAPD molecular markers

Agriculture and Biology Journal of North America ◽

10.5251/abjna.2011.2.2.197.202 ◽

2011 ◽

Vol 2 (2) ◽

pp. 197-202 ◽

Cited By ~ 1

Author(s):

Amin Baghizadeh ◽

Sahar Khosravi

Keyword(s):

Genetic Diversity ◽

Molecular Markers ◽

Diversity Assessment

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Genetic Diversities within Camellia Species Confirmed by Random Amplified Polymorphic DNA (RAPD) Markers

HortScience ◽

10.21273/hortsci.40.4.1105a ◽

2005 ◽

Vol 40 (4) ◽

pp. 1105A-1105

Author(s):

Lianghong Chen ◽

Shizhou Wang ◽

Mack Nelson

Keyword(s):

Genetic Diversity ◽

Genetic Variation ◽

Molecular Markers ◽

Rapd Markers ◽

Random Amplified Polymorphic Dna ◽

Camellia Japonica ◽

Average Percentage ◽

Study Research ◽

Camellia Reticulata ◽

Camellia Species

In this study research was conducted to evaluate the feasibility of characterizing genetic variation within camellia species using random amplified polymorphic DNAs (RAPD) markers. Eight varieties of species Camellia japonica and four varieties of species Camellia reticulata, provided by the America Camellia Society, Fort Valley, Ga., were investigated. RAPD profiles generated by five selected 10-based random primers (out of 20 primers) exhibited distinct patterns of amplified bands for all 12 tested varieties. A total of 344 bands were produced among the eight varieties of species C. japonica, with an average of 8.6 bands, ranging from 220 to 2072 bp in size, scored per primer. Among the 344 amplified bands, 74.4% of the bands presented polymorphic. The four varieties of species C. reticulata produced a total of 180 markers, with an average percentage of 57.8% polymorphisms. The amplified bands were in the range of 236–1760 bp. An average of nine amplified bands was generated per primer. The large percentages of polymorphisms displayed among 12 varieties within the two different species indicate that the expected genetic diversity among varieties within camellia species existed. It was concluded that the RAPD molecular markers are capable of revealing appreciable levels of genetic variation within camellia species.

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Genetic Diversity Assessment of Lines and Varieties in Winter Rapeseed (Brassica napus L.) using RAPD and SSR Molecular Markers

Journal of Crop Breeding ◽

10.18869/acadpub.jcb.8.17.139 ◽

2016 ◽

Vol 8 (17) ◽

pp. 139-131 ◽

Cited By ~ 2

Author(s):

حسن هدایتی مرزونی ◽

حبیب اله سمیع زاده لاهیجی ◽

◽

Keyword(s):

Genetic Diversity ◽

Molecular Markers ◽

Brassica Napus ◽

Brassica Napus L ◽

Diversity Assessment ◽

Winter Rapeseed

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Genetic diversity assessment of cucumber (Cucumis sativus L.) genotypes using molecular markers

Electronic Journal of Plant Breeding ◽

10.5958/0975-928x.2017.00136.3 ◽

2017 ◽

Vol 8 (3) ◽

pp. 986 ◽

Cited By ~ 1

Author(s):

Shailaja Punetha ◽

D.K. Singh ◽

N.K. Singh

Keyword(s):

Genetic Diversity ◽

Molecular Markers ◽

Cucumis Sativus ◽

Cucumis Sativus L ◽

Diversity Assessment

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Protein-Based Genetic Diversity Assessment of Tilapia guineensis and Sarotherodon melanotheron Populations from South-West Nigerian Coastal Waters

Asian Journal of Fisheries and Aquatic Research ◽

10.9734/ajfar/2020/v8i230134 ◽

2020 ◽

pp. 9-15

Author(s):

E. A. Ukenye ◽

I. Megbowon ◽

M. M. A. Akinwale ◽

M. A. Fowora ◽

I. Chidume ◽

...

Keyword(s):

Genetic Diversity ◽

Genetic Variation ◽

Genetic Similarity ◽

Polyacrylamide Gel Electrophoresis ◽

Sodium Dodecyl ◽

Group Method ◽

Cichlid Species ◽

Diversity Assessment ◽

Sds Page ◽

Sarotherodon Melanotheron

The present study was carried out to investigate the genetic differences in the Protein banding pattern of Tilapia guineensis and Sarotherodon melanotheron populations in Southwest Nigeria using Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE). Four populations of Tilapia guineensis and three populations of Sarotherodon melanotheron from Ondo and Lagos states were considered for the study. The sarcoplasmic protein of the studied Cichlid species resolved on 12% SDS-PAGE revealed variations in their genetic diversity indices (number of alleles, shanon information index, heterozygosity and percentage polymorphism). T. guineensis had more proteins and higher genetic diversity as was revealed by the genetic diversity parameters and was found to be more polymorphic with a percentage polymorphism of 78.57% than S. melanotheron (57.14%). The two species had similarity coefficient of 0.82 indicating high genetic similarity between them. UPGMA (Unweighted Pair Group Method with Arithmetic Mean) dendrogram also revealed some level of genetic similarity between the studied populations and among the two species. Analysis of molecular variance (AMOVA) confirmed the low genetic variation among the populations of the cichlid species and demonstrated that genetic variation was mostly within populations in both species. It is established from the study that Tilapia guineensis had higher genetic diversity than Sarotherodon melanotheron and the two species are closely related. Further study involving molecular markers is encouraged to complement this finding.

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