Analysis of Functional Components of Burdock (Arctium lappa root) Standard Water Extract and its Aphrodisiac Effect in Experimental Rats

2020 ◽  
Vol 42 (1) ◽  
pp. 94-94
Author(s):  
Guo qing Ren Guo qing Ren ◽  
Xiao ning Luan and De qiang Dou Xiao ning Luan and De qiang Dou
Keyword(s):  
Amino Acids ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Hplc Analysis ◽  
Water Extract ◽  
Active Components ◽  
Arctium Lappa ◽  
Amino Acid Analyzer ◽  
Functional Components

High performance liquid chromatography (HPLC) analysis showed that the content of chlorogenic acid in burdock standard water extract (BSWE) was about 1.22and#177;0.07 mg/g and no araciin and arctigenin was detected. The inulin was determined to be 174.33and#177;3.68 mg/g in BSWE by colorimetry method. The amino acids were analyzed by the amino acid analyzer and showed that asparagine and arginine are higher and determined to be 3021.00and#177;13.53mg/100g, 2042.33and#177;8.62mg/100g in BSWE, respectively. The results of aphrodisiac pharmacological experiments showed that the number of riding, insertions and the latency of rats in BSWE groups showed significant differences as compared with control, at the 7th day and 15th day after drug administration. Further study indicated that the aphrodisiac effect of BSWE is mostly associated with its regulation on NO, sialic acid and antioxidative ways and arginine should be one of the active components in BSWE.

Determination of sulfur amino acids in feedstuffs by high performance liquid chromatography coupled with pre-column derivatization

2011 ◽  
Vol 29 (3) ◽  
pp. 239-243 ◽  
Author(s):  
Shuxin YU ◽  
Si FENG ◽  
Yuanshe SUN ◽  
Tao TANG ◽  
Jing HAN ◽  
...  
Keyword(s):  
Amino Acids ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Sulfur Amino Acids

Simultaneous Determination of Six Components in Jingzhiguanxin Tablet by High-Performance Liquid Chromatography

2019 ◽  
Vol 15 (2) ◽  
pp. 130-137
Author(s):  
Hui Jiang ◽  
Lianhao Fu ◽  
Yu Wang ◽  
Shaozhi Wang ◽  
Xiaoxu Zhang ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Gradient Elution ◽  
Tanshinone Iia ◽  
Array Detector ◽  
Control Analysis ◽  
Active Components ◽  
Quality Control Analysis

Background: Jingzhiguanxin (JZGX) tablet, a traditional Chinese prescription, is commonly used for treating coronary heart disease and angina pectoris in the clinic. There are six active components (Danshensu (DSS), Protocatechuic aldehyde (PD), Paeoniflorin (PF), Ferulic acid (FA), Salvianolic acid B (Sal B) and Tanshinone IIA (TA)) in JZGX tablet. </P><P> Objective: In this paper, a simple and reliable method was used for simultaneous determining the six active components by high-performance liquid chromatography coupled with diode array detector (HPLC-DAD). Methods: These six active components were separated on an Agilent Zorbax Eclipse XDB-C18 column (150 mmx4.6 mm, 5 µm) at 30 °C. Acetonitrile (A), methanol (B) and 0.5% H3PO4 aqueous solution (C) were used as mobile phase for gradient elution. The flow rate was 1 mL/min and the detection wavelengths were set at 280 nm for DSS, PD and Sal B, 230 nm for PF, 320 nm for FA and 270 nm for TA, respectively. Results: All of the six components showed good linearity regressions (r2≥0.9997) in the detected concentration range. The recovery rates and coefficient of variation (CV) for all analytes were 98.66%- 100.18% and 0.75%-1.89%, respectively. This method was successfully applied to simultaneously determine the six components in JZGX tablet from different batches and manufacturers. Conclusion: The validated method can be used in routine quality control analysis of JZGX tablet without any interference.

HPLC Analysis of Oxytetracycline Residues in Honey

1986 ◽  
Vol 49 (5) ◽  
pp. 383-388 ◽  
Author(s):  
PETER SPORNS ◽  
SUET KWAN ◽  
LAWRENCE A. ROTH
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Aqueous Solutions ◽  
High Performance ◽  
Hplc Analysis ◽  
Extraction Procedure ◽  
Hplc Method ◽  
Limits Of Detection ◽  
Ph Values ◽  
Double Extraction

Oxytetracycline (OTC), also known commercially as Terramycin, was determined to be more stable in honey than in buffered aqueous solutions at similar pH values and temperatures. A rapid high performance liquid chromatography (HPLC) method was developed to detect and quantitate OTC using a 1:1 dilution (wt/wt) of honey samples in water. Using 355 nm as the wavelength of detection, amounts as low as 0.5 μg/ml could be detected in the above solution. The limits of detection were lowered considerably by a double extraction procedure.

Chiral discrimination of Dns- and unmodified d,l-amino acids by copper(II) complexes of terdentate ligands in high-performance liquid chromatography

1998 ◽  
Vol 829 (1-2) ◽  
pp. 101-113 ◽  
Author(s):  
Gianni Galaverna ◽  
Roberto Corradini ◽  
Arnaldo Dossena ◽  
Emma Chiavaro ◽  
Rosangela Marchelli ◽  
...  
Keyword(s):  
Amino Acids ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Chiral Discrimination
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