Determination of Thiamine Hydrochloride in Commonly used food Materials-Rice by UV-Spectrophotometric Methods

Author(s):  
Senthilraj. R ◽  
Harikrishnan. N ◽  
Swetha. V ◽  
Kavitha. S ◽  
Gous Basha. T ◽  
...  

Vitamins and their coenzymes roles are indispensable for normal functioning of biochemical metabolic pathways. The water soluble vitamin thiamine plays an important role in energy metabolism and its continuous intake is very essential. The source of vitamins can be obtained from many foods like milk, legumes, peas and rice and which is depends on availability, cost. Although most part of the south Indians especially in Tamilnadu the people predominantly depends on rice as a major source of food. So in order to identify the recommended dietary allowance of thiamine the following rice samples were collected from provisional store in Chennai. The collected five rice samples were Deluxe, Bapatla, Karnataka, Rupali ponni rice and Basmati rice. The concentration of thiamine present in the rice was estimated by UV Spectrophotometrically at 246 nm using 0.1M hydrochloride as blank. In order to measure the actual content the high heat and pressure not used and washing is not used. Among the tested 10gm of rice samples, Rupali ponni rice has127 µg which is very less quantity and Bapatla, Deluxe, Karnataka ponni rice has 420 to 430 µg. The high content of was present in Basmati rice-983 µg. Anyhow normal consuming rice quantity is more sufficient to meet Recommended Daily Allowance- RDA level but more washing of rice, prolong boiling will reduce the amount of thiamine content in rice during usage.

2017 ◽  
Vol 100 (5) ◽  
pp. 1511-1515
Author(s):  
Frédéric Martin ◽  
Liliane Meyer ◽  
Konstantinos Zelianos ◽  
Esther Campos Gimenez

Abstract The purpose of this work was to understand low recoveries of thiamine (vitamin B1) when extracted from cocoa-containing beverage powders fortified with water-soluble vitamin B1, and to develop and validate a new procedure to improve these results. Based on the literature, previous trials have focused on two main factors: pH value prior to paper filtration and the need for solid-phase extraction (SPE) clean up. We demonstrate that by following European Standard EN 14122, recovery of thiaminein cocoa-containing beverage powders is low and dependent on the test portion (86 and 72% for 0.5 and 1.5 g test portions, respectively). Our improved method resolved this problem by keeping the pH low (around 1) prior to paper filtration, leading to a 96.3% recovery and high precision (RSDr of 3.5%). The use of strong cation-exchange SPE cartridges for cleanup prior to the thiamine oxidation reaction proved to be essential. A comparison between our improved method and EN 14122 on nine cocoa-containing beverage samples available on local market from different manufacturers showed a systematic increase in thiamine content (up to 70%) when the improved methodwas applied. The highest difference was observed forthe sample that contained the highest amount of cocoa. However, for beverage powders that contained bothcocoa and milk, no difference was observed.


2021 ◽  
Vol 17 ◽  
Author(s):  
Rana Amir ◽  
Hind Hadi

Background: Thiamine hydrochloride (THY), also known as vitamin B1, is a water soluble vitamin usually found in food, and considered as a dietary supplement and treatment in case of vitamin deficiency for example in malabsorption and chronic diarrhea. Objective: Rapid, green and sensitive methods for the assay of vitamin B1 (thiamine hydrochloride, THY) in pharmaceutical forms, using normal and reverse flow injection manifolds and sulfonamide drugs as green and safe reagents were reported. Sulfamethoxazole and sulfadimidine drugs were used rather than toxic and expensive reagents. Methods: nFIA and rFIA manifolds were used for automated the reaction of THY with diazotized sulfamethoxazole (DSMZ) and diazotized sulfadimidine (DSDM) in alkaline medium respectively. The absorbance of the resultant red-orange azo dyes was monitored spectrophotometrically at λmax 494 and 496 nm for both methods respectively. Results: The linearity of the suggested methods was in the ranges 2–80 µg/mL (LOD 0.69 µg/mL, % RSD 0.32, n=6) for nFIA method and 0.5-70 µg/mL (LOD 0.29 µg/mL, %RSD 0.85, n=6) for rFIA. Sampling frequency was 84 and 51 injections per hour for nFIA and rFIA methods respectively. Chemical and physical variables for both methods were studied carefully. Conclusion: Both flow injection modes were effectively applied in assay of THY in its pharmaceutical forms. The results were compared with those of standard pharmacopeia method and the statistical analysis indicated insignificant differences in accuracy and precision between the methods.


2020 ◽  
Vol 5 (12) ◽  
pp. 2160-2167 ◽  
Author(s):  
Nora Schwotzer ◽  
Michiko Kanemitsu ◽  
Sebastien Kissling ◽  
Roger Darioli ◽  
Mohammed Benghezal ◽  
...  

Nutrients ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1913
Author(s):  
Alessandra Vincenti ◽  
Laura Bertuzzo ◽  
Antonio Limitone ◽  
Giuseppe D’Antona ◽  
Hellas Cena

Vitamin B12 (also known as cobalamin) is an essential water-soluble vitamin that plays a pivotal role for several physiologic functions during one’s lifespan. Only certain microorganisms are able to synthetize B12, thus humans obtain cobalamin exclusively from their diet, specifically from animal-derived foods. Specific sub-group populations are at risk of vitamin B12 subclinical deficiency due to different factors including poor intake of animal source foods and age-dependent decrease in the capacity of intestinal B12 uptake. Consumption of animal products produces some negative health issues and negatively impacts sustainability while a plant-based diet increases the risk of B12 deficiency. Taking a cue from the aforementioned considerations, this narrative review aims to summarize facts about B12 deficiency and the burden of inadequate dietary intake in elderly population, as well as to discuss sustainable approaches to vitamin B12 deficiency in aging population.


Author(s):  
Zheng Zhang ◽  
Nicharee Wisuthiphaet ◽  
Nitin Nitin ◽  
Luxin Wang ◽  
Ryan Kawakita ◽  
...  

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