scholarly journals Sterilization and Callus Formation of Rubber Meristem (Hevea brasiliensis Muell. Arg.)

2018 ◽  
Vol 6 (4) ◽  
pp. 1
Author(s):  
Dwi Sucianingtyas Sukamto ◽  
Lila Maharani ◽  
Siti Amalia ◽  
Sholeh Avivi ◽  
Didik Pudji Restanto
Keyword(s):  
Tissue Culture ◽  
Callus Induction ◽  
Hevea Brasiliensis ◽  
Callus Formation ◽  
Rubber Plantation ◽  
Sterile Water ◽  
Mass Propagation ◽  
Culture Techniques ◽  
Embryonic Callus ◽  
Tissue Culture Techniques

Rubber plant (Hevea brasiliensis Muell Arg) is one of the important plantation commodities in Indonesia because of its role as a source of income. It stimulates the economic growth around the rubber plantation area. The propagation of rubber is still using conventional methods like grafting. The technique of tissue culture through callus induction is one of the alternatives of mass propagation of rubber seedling with quick and efficient time. The sterilization method is very important to determine the success of tissue culture techniques. Therefore, the aim of this research is to know the best method of sterilization and callus formation in rubber explants. The basic media used were WPM and MS, with BAP of 2 ppm and NAA 0.1 ppm. The best result of sterilization is by soaking 5% fungicide solution for 5 minutes, 5% Clorox solution for 15 minutes, betadin 10% solution for 5 minutes, and finally it rinsed with sterile water three times. The best medium uses WPM medium for callus induction, with 0.5 cm callus length and embryonic callus. In contrast, the MS medium has 0.4 cm callus length and non embryonic callus.

scholarly journals Perbandingan Konsentrasi ZPT (BAP dan NAA) Pada Media MS Terhadap Pertumbuhan Kalus Eksplan Daun Muda Tanaman Karet (Hevea brasiliensis Muell. Arg)

bionature ◽  
2018 ◽  
Vol 18 (2) ◽  
Author(s):  
Dwi Sucianingtyas Sukamto ◽  
Lila Maharani ◽  
Inget Puji Lestari
Keyword(s):  
Hevea Brasiliensis ◽  
Callus Formation ◽  
Colonial Period ◽  
Seed Plants ◽  
Ms Medium ◽  
Plant Leaves ◽  
Cross Sectional ◽  
Culture Techniques ◽  
The World ◽  
Tissue Culture Techniques

Abstract. Rubber is known in Indonesia since the Dutch colonial period. Indonesia is the second largest natural rubber producer in the world after Thailand, rubber production contributes greatly to the Indonesian economy. Propagation of rubber seedlings to date is still done by grafting is by using the eyes of buds and seed plants, one of the alternatives to meet the demand for rubber seedlings that are increasing and not dependent on the season and to produce the clonal rootstock is homogeneous by tissue culture techniques. This study aims to find out the response of callus formation of young leaf rubber plant leaves (Hevea brasiliensis Muell, Arg) on MS medium with different concentration of ZPT (BAP and NAA). This research uses Cross Sectional type of observation research, with combination of MS and ZPT media used ie MS with BAP 1.5 ppm + NAA 0,05 ppm, BAP 2 ppm + NAA 0,1 ppm, BAP 2.5 ppm + NAA 0.2 ppm. The parameters observed were the forming of callus, callus texture and callus color for 21 days. Based on the results of the study showed that MS treatment with ZPT concentration (BAP 1.5 ppm + NAA 0.05ppm) gave the best result for callus formation response with highest explanation percentage of 0,06%.Keywords. Calli, Concentration ZPT, Hevea brasiliensis Muell.Arg. 

scholarly journals The regeneration of Acer rubrum L. "October Glory" through embryonic callus

2020 ◽  
Author(s):  
Chongwen Dai ◽  
Yang-yang YAN ◽  
Yumin Liu ◽  
Ya-min LIU ◽  
Yuan-wei DENG ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Callus Induction ◽  
Callus Formation ◽  
Acer Rubrum ◽  
Adventitious Bud ◽  
Naphthaleneacetic Acid ◽  
Stem Segment ◽  
Embryonic Callus ◽  
Bud Induction ◽  
Culture Process

Abstract Background: Tissue culture and rapid propagation technology is an important way to solve the difficulties of plant propagation. This experiment aims to explore the appropriate conditions at each stage of the red maple’s tissue culture process and to obtain plantlets, thus providing a theoretical basis for the establishment of the red maple’s tissue culture system. Results: The results showed that the stem segment is the most suitable explant for inducing embryogenic callus. The MS (Murashige&Skoog) +0.8 mg/L TDZ (Thidiazuron) +1.0 mg/L 6-BA (6-Benzylaminopurine) +0.5 mg/L IAA(Indole-3-acetic acid) +35 g/L sucrose+7.5 g/L semi-fixed medium was the best for callus formation. When selecting type Ⅵ callus as embryonic callus induction material, MS+0.6 mg/L TDZ+0.5 mg/L 6-BA +2.0 mg/L IAA +35 g/L sucrose+7.5 g/L semi-fixed medium can get embryonic callus. The optimal medium for adventitious bud induction is MS+1.0 mg/L TDZ+3.0 mg/L 6-BA+0.2 mg/L NAA (1-Naphthaleneacetic acid)+1.2 mg/L IAA+35 g/L sucrose+7.5 g/L semi-fixed medium. The induction rate of adventitious roots in MS+0.6 mg/L TDZ+1.0 mg/L 6-BA+3 mg/L NAA+35 g/L sucrose+7.5 g/L semi-fixed medium was the highest, reaching 76%. Conclusions: In the course of our research, we found that PGRs play an important role in the callus induction stage, and the effect of TDZ is particularly obvious; The callus cells grow and proliferate according to the "S" growth curve, and can be sub-cultured when the highest growth point is reached to maintain the rapid proliferation of the callus cells and to avoid inactivation of callus caused by tight niche.

Cytogenetic Analysis of 39 Prostate Carcinomas and Evaluation of Short Term Tissue Culture Techniques

1998 ◽  
Vol 101 (2) ◽  
pp. 116-122 ◽  
Author(s):  
Josée J König ◽  
Wilma Teubel ◽  
Eric Kamst ◽  
Johannes C Romijn ◽  
Fritz H Schröder ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Cytogenetic Analysis ◽  
Short Term ◽  
Culture Techniques ◽  
Prostate Carcinomas ◽  
Tissue Culture Techniques

DEVELOPMENT OF NEOLAMARCKIA CADAMBA (KELEMPAYAN) TISSUE CULTURE TECHNIQUES FOR SUSTAINABLE SUPPLY OF PLANTING MATERIALS FOR COMMERCIAL PLANTATION

2015 ◽  
Vol 77 (24) ◽  
Author(s):  
Siti Suhaila A. Rahman ◽  
Norwati Muhammad ◽  
Nor Hasnida Hassan ◽  
Haliza Ismail ◽  
Nazirah Abdullah ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Mass Production ◽  
Nodal Segments ◽  
Direct Organogenesis ◽  
Timber Species ◽  
Culture Techniques ◽  
Commercial Plantation ◽  
Planting Materials ◽  
Tissue Culture Techniques

Neolamarckia cadamba (kelempayan) is a multipurpose and fast growing timber species. The tree is grown for timber, paper-making and as ornamental plant. It is reported that its barks and leaves possesed medicinal values and its flowers are used in perfumes. The species is also known to be suitable for plywood, packing case, toys and short-fibred pulp. Therefore, mass production of high quality planting material of N. cadamba is important to support plantation program of this species. Here we presented mass production of N. cadamba through tissue culture techniques. Nodal segments derived from in vitro germinated seeds were used and induced direct organogenesis to produce shoots and roots using MS media (1962) and plant growth regulators (BAP and IBA) that are relatively cheaper than previously used methods. The tissue culture technique of N. cadamba developed may help in ensuring supply of planting materials that are feasible for commercial plantation purposes.

scholarly journals The effect of NAA and coconut water combination on garlic (Allium sativum L.) tissue culture

2021 ◽  
Vol 905 (1) ◽  
pp. 012036
Author(s):  
Nandariyah ◽  
L S Mahmudah ◽  
R B Arniputri ◽  
A T Sakya
Keyword(s):  
Tissue Culture ◽  
Coconut Water ◽  
Emergence Time ◽  
Shoot Height ◽  
Culture Techniques ◽  
Randomized Design ◽  
Two Factors ◽  
Number Of Leaves ◽  
Tissue Culture Techniques ◽  
Number Of Shoots

Abstract Tissue culture techniques can increase the number of garlic seedlings. The purpose of this research is to determine the effect of NAA and coconut water in increasing the number of garlic seeds. This research used a Completely Randomized Design of two factors. The treatment used is NAA with concentrations of 0 ppm, 0.5 ppm, 1 ppm, 1.5 ppm, and coconut water concentrations of 0%, 10%, 20%. The variables observed were shoot emergence time, root emergence time, number of shoots, number of roots, number of leaves, shoot height, root length, and number of plantlets. The results showed that the addition of coconut water 20% without the addition of NAA in 1 bulb can produce 3.33 planlets and the results of explant propagation in 1 bulb can produce the number of shoots as many as 15.33 shoots. Giving coconut water with concentrations of 10% and 20% can increase the number of leaves, shoot height, and some planlets. The concentration of NAA 0.5 ppm can accelerate the root emergence time on garlic explant.

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