Perbandingan Konsentrasi  ZPT (BAP dan NAA) Pada Media MS Terhadap Pertumbuhan Kalus Eksplan Daun Muda Tanaman Karet (Hevea brasiliensis Muell. Arg)

Abstract. Rubber is known in Indonesia since the Dutch colonial period. Indonesia is the second largest natural rubber producer in the world after Thailand, rubber production contributes greatly to the Indonesian economy. Propagation of rubber seedlings to date is still done by grafting is by using the eyes of buds and seed plants, one of the alternatives to meet the demand for rubber seedlings that are increasing and not dependent on the season and to produce the clonal rootstock is homogeneous by tissue culture techniques. This study aims to find out the response of callus formation of young leaf rubber plant leaves (Hevea brasiliensis Muell, Arg) on MS medium with different concentration of ZPT (BAP and NAA). This research uses Cross Sectional type of observation research, with combination of MS and ZPT media used ie MS with BAP 1.5 ppm + NAA 0,05 ppm, BAP 2 ppm + NAA 0,1 ppm, BAP 2.5 ppm + NAA 0.2 ppm. The parameters observed were the forming of callus, callus texture and callus color for 21 days. Based on the results of the study showed that MS treatment with ZPT concentration (BAP 1.5 ppm + NAA 0.05ppm) gave the best result for callus formation response with highest explanation percentage of 0,06%.Keywords. Calli, Concentration ZPT, Hevea brasiliensis Muell.Arg.

Download Full-text

Sterilization and Callus Formation of Rubber Meristem (Hevea brasiliensis Muell. Arg.)

Journal of Agricultural Studies ◽

10.5296/jas.v6i4.13796 ◽

2018 ◽

Vol 6 (4) ◽

pp. 1

Author(s):

Dwi Sucianingtyas Sukamto ◽

Lila Maharani ◽

Siti Amalia ◽

Sholeh Avivi ◽

Didik Pudji Restanto

Keyword(s):

Tissue Culture ◽

Callus Induction ◽

Hevea Brasiliensis ◽

Callus Formation ◽

Rubber Plantation ◽

Sterile Water ◽

Mass Propagation ◽

Culture Techniques ◽

Embryonic Callus ◽

Tissue Culture Techniques

Rubber plant (Hevea brasiliensis Muell Arg) is one of the important plantation commodities in Indonesia because of its role as a source of income. It stimulates the economic growth around the rubber plantation area. The propagation of rubber is still using conventional methods like grafting. The technique of tissue culture through callus induction is one of the alternatives of mass propagation of rubber seedling with quick and efficient time. The sterilization method is very important to determine the success of tissue culture techniques. Therefore, the aim of this research is to know the best method of sterilization and callus formation in rubber explants. The basic media used were WPM and MS, with BAP of 2 ppm and NAA 0.1 ppm. The best result of sterilization is by soaking 5% fungicide solution for 5 minutes, 5% Clorox solution for 15 minutes, betadin 10% solution for 5 minutes, and finally it rinsed with sterile water three times. The best medium uses WPM medium for callus induction, with 0.5 cm callus length and embryonic callus. In contrast, the MS medium has 0.4 cm callus length and non embryonic callus.

Download Full-text

334 Meristem Isolation of Garlic (Allium sativum L.) Cultivars Sao Marcos and Sao Valentim

HortScience ◽

10.21273/hortsci.35.3.449d ◽

2000 ◽

Vol 35 (3) ◽

pp. 449D-449

Author(s):

Nilvane T.G. Müller ◽

Gerson R. de L. Fortes ◽

Gioconda C. Nascimento ◽

Julio Daniels

Keyword(s):

Allium Sativum ◽

Callus Formation ◽

New Variety ◽

Culture Techniques ◽

Mediterranean Countries ◽

Significant Difference ◽

The Mean ◽

Shoot Base ◽

Tissue Culture Techniques ◽

Number Of Shoots

Garlic (Allium sativum L.) belongs to the Alliaceae family and originated from Asia and Mediterranean countries. Their bulblets are rich in starch and aromatic substances. The rate of garlic propagation in field conditions takes several years for the production of a certain number of seed bulbs for the release of a new variety. The use of tissue culture techniques is a useful tool for overcoming this problem. The aim of this work was to increase the mean number of shoots derived from the meristem isolation and to verify the percentage of callus formation and to analyze vigor of the material. The initial meristems were inoculated in a salt and vitamin B5 media except for the iron element, which was provided by MS medium added to in mg·L-1: myo-inositol (100.0), nicotinic acid (1.0), piridoxine (1.0), thiamine (10.0), sucrose (20.0 g·L-1), agar (6.0 g·L-1). BAP and TDZ were added at: 0.0; 1.0; 1.5; 2.0; and 2.5 μM This material remained in a growth room for a 16-h photoperiod, radiation of 20 μMol·m-2·s-1 and 25 °C for 40 days. Although `Sao Marcos' produced more vigorous shoots, no significant difference was found for the mean number of shoots. `Sao Valentim' cultivar shows more callus at the shoot base, making this cultivar more prone to somaclonal variation On the other hand, BAP estimulates the appearance of callus, but it has been shown that this is cultivar-dependent.

Download Full-text

The Effect of different concentration of growth regulater on micropropagtion of Lisianthus (Eustoma grandiflora)

Baghdad Science Journal ◽

10.21123/bsj.9.3.378-385 ◽

2012 ◽

Vol 9 (3) ◽

pp. 378-385

Author(s):

Baghdad Science Journal

Keyword(s):

Culture Medium ◽

Shoot Multiplication ◽

Shoot Length ◽

Peat Moss ◽

Ms Medium ◽

River Sand ◽

Culture Techniques ◽

Leaf Discs ◽

Shoot Number ◽

Tissue Culture Techniques

Plant tissue culture techniques were exploited for the micropropigation of Lisianthus (Eustoma grandiflora). Different concentrations of Benzyl adenine (BA), 6- Furfural amino purine (Kinetin), Indol butyric acid (IBA), were investigated in their effects at different micropropagation stages. Three explants (apical shoots, internodes, leaf discs) were used in this study. The effect of the interaction between BA and IBA on shoot multiplication was investigated in increasing the number of shoots on explants. Rooting was also studied after inclusion of IBA and NAA to Murashige and Skoog, 1962 culture medium (MS). During acclimatization stage, different ratios of river sand and peat moss as agricultural media were tested and plantlets survival was recorded. Maximum mean shoot number was recorded when leaf discs were cultured on MS medium supplemented with 0.3 mg/l of BA giving 6.8 shoots. Highest mean shoot length was obtained when BA at 0.1 mg/l was added to the culture medium giving 1.16 cm. Internodes achieved maximum mean shoot length reached 1.6 cm in the medium supplemented with 0.1 mg/l of BA, while the same concentration of KIN extended the mean shoot length up to 1.6 cm. Shoots that transferred to MS medium containing 1.0 mg/l of IBA achieved the highest root number (5.5) with mean length of 2.5 cm and achieved 70% rooting percentage. Plantlets were acclimatized achieving 90% survival when transferred to agricultural medium consisted of rivers and peat moss at 2:1 v/v ratio.

Download Full-text

Dwindling status of Epimedium Elatum (Morren & Decne) and its geographical distribution in Kashmir Himalaya, India

Current Botany ◽

10.25081/cb.2018.v9.20180106 ◽

2018 ◽

pp. 47-52

Keyword(s):

Medicinal Plant ◽

Biological Activities ◽

Conservation Status ◽

Natural Populations ◽

Culture Techniques ◽

Kashmir Himalayas ◽

Near Future ◽

First Time ◽

Tissue Culture Techniques

Epimedium elatum (Morren & Decne) of family Berberidaceace is a rare perennial medicinal plant, endemic to high altitude forests of Northwestern Himalayas in India. Ethnobotanically, it has been used as an ingredient for treatment of bone-joint disorders, impotence and kidney disorders in Kashmir Himalayas. Phytochemically, it is rich in Epimedin ABC and Icariin; all of these have been demonstrated to possess remarkable biological activities like PDE-5 inhibition (treatment of erectile dysfunction), anticancer, antiosteoporosis antioxidant and antiviral properties. The present investigation reports its traditional usage, comprehensive distribution and conservation status from twenty ecogeographical regions in Kashmir Himalayas, India. The species was reported from Gurez valley for the first time. Numerous threats like excessive grazing, deforestration, habitat fragmentation, tourism encroachment, landslides and excessive exploitation have decreased its natural populations in most of the surveyed habitats. Consequently, its existence may become threatened in near future if timely conservation steps are not taken immediately by concerned stakeholders involved in medicinal plant research. Moreover, use of plant tissue culture techniques is recommended for development of its in vitro propagation protocols. Therefore, introduction of this medicinal plant in botanical gardens, protected sites and development of monitoring programmes are needed for its immediate conservation in Northwestern Himalayas, India.

Download Full-text

HUBUNGAN ANTARA PENDIDIKAN DAN STATUS EKONOMI IBU DENGAN KEJADIAN DIARE PADA BALITA DI PUSKESMAS SEKIP PALEMBANG TAHUN 2014

Jurnal Kebidanan : Jurnal Medical Science Ilmu Kesehatan Akademi Kebidanan Budi Mulia Palembang ◽

10.35325/kebidanan.v4i2.65 ◽

2019 ◽

Vol 4 (2) ◽

Author(s):

Wulan Citra Sari Wulan Citra Sari

Keyword(s):

Economic Status ◽

Chi Square ◽

Cross Sectional ◽

Diarrhea Case ◽

The World ◽

Diarrhea Disease ◽

United Nation ◽

Research Show ◽

Improve Health ◽

Children Under 5

ABSTRACT Diarrhea is cause the number one of a death in the world. The united Nation Children’s Found (UNICEF) thinks about every 30 second there one a child die because diarrhea. Diarrhea disease still be a problem for society healthy in Indonesia well reviewed of morbidity and mortality ant the mark of deaths and “Kejadian Luar Biasa (KLB)” that caused. Accrodin to WHO the mark of diarrhea illness 2010 is 411 patients per 1.000 population. The digit of death diarrhea is 14% from the digit of death over all. The purpose of this research to know a connection between education and mother economic status with toodler (children under 5 years old) diarrhea case at Puskesmas Sekip Palembang in February 2014. Desaign of this research to survey analitic by do coloser Cross Sectional in Accidental Sampling tehnic which one the variable who get research, include education and mothers economic status. The sampel in this research in 33 mothers who come by bring their toodler to MTBS Puskesmas Sekip Palembang at February 2014. Each variabel who get research, then get analysis by use analysis Chi-Square by decisios X² table (3,481). The result of this research show that the respondent by diarrhea as many is 15 respondent (45,5%) and didn’t diarrhea 18 respondent (54,5%). This of high education 13 repondent (39,4%) and less education is many as 20 respondent (60,6%), the respondent based to the high economic status as many as 13 respondent (39,4%). From statistic test Chi-Square show that, there a connection between education with diarrhea case in X² count = 4,313, and there a connection between mothers economic status with case diarrhea at X² count= 4,917. From the results of this study are expected to further improve health education, especially regarding the incidence of diarrhea in infants to mothers who have young children understand more about the incidence of diarrhea. ABSTRAK Diare adalah penyebab nomor satu kematian di dunia. The United Nations Children’s Fund (UNICEF) memperkirakan bahwa setiap 30 detik ada anak meninggal karena diare. Penyakit diare masih merupakan masalah kesehatan masyarakat di Indonesia baik ditinjau dari angka kesakitan dan angka kematian serta kejadian luar biasa (KLB) yang ditimbulkan. MenurutWHO angka kesakitan diare pada tahun 2010 yaitu 411 penderita per 1000 penduduk. Angka kematian diare 14% dari angka kematian balita secara keseluruhan. Tujuan penelitian ini untuk mengetahui hubungan antara pendidikan dan status ekonomi ibu dengan kejadian diare pada balita di Puskesmas SekipPalembang pada bulan Februari Tahun 2014. Desain penelitian ini adalah survey analitik dengan pendekatan Cross Sectional dalam teknik Accidental Sampling dimana variableyang diteliti meliputi pendidikan dan status ekonomi ibu. Sampel pada penelitian ini adalah 33ibu yang datang membawa anak balitanya berobat ke MTBS puskesmas Sekip Palembang pada bulan FebruariTahun 2014. Masing-masing variabel yang diteliti kemudian dianalisa dengan menggunakan analisis Chi-Square dengan ketetapan X² Tabel (3,481). Hasil penelitian ini menunjukan bahwa responden dengan diare sebanyak 15 responden (45,5%) dan tidak diare 18 responden (54,5%), responden berdasarkan pendidikan tinggisebanyak 13responden (39,4%) dan pendidikan rendah sebanyak 20 responden (60,6%), responden berdasarkan status ekonomi tinggi sebanyak 20 responden (60,6%) dan yang status ekonomi rendah sebanyak 13 responden (39,4%). Dari uji statistik Chi-Square menunjukkan ada hubungan antara pendidikan dengan kejadian diare pada X²hitung= 4,313, dan ada hubungan antara status ekonomi ibu dengan kejadian diare pada X²hitung= 4,917. Dari hasil penelitian ini diharapkan agar lebih meningkatkan penyuluhan kesehatan khususnya tentang kejadian diare pada balita agar ibu yang mempunyai balita lebih memahami tentang kejadian diare.

Download Full-text

“These Shall Come from Far”

10.1093/oso/9780190665135.003.0004 ◽

2017 ◽

Author(s):

Kristina Bross

Keyword(s):

Material Culture ◽

New England ◽

Colonial Period ◽

Global Network ◽

The World ◽

Basket Maker

Chapter 3 analyzes English claims to a central role in a global network of indigenous and English people connected by faith around the world, claims made manifest in Of the Conversion of Five Thousand Nine Hundred Indians on the Island of Formosa, a 1650 publication by Baptist minister Henry Jessey, printed by radical bookseller Hannah Allen. It reports on Dutch missions in Taiwan, comparing them with evangelism efforts in New England. The coda considers the experiences of an Algonquian woman who is unnamed in Jessey’s tract but is identified as a basket maker, speculating on the meaning she may have encoded in her basket designs. Though we cannot “read” them directly, the fact that she made them, coupled with the provocative arguments offered by recent scholars about Native material culture in the colonial period, enables us to reconsider the print archive in which she appears.

Download Full-text

Psychological impact in the time of COVID-19: A cross-sectional population survey study during confinement

Journal of Health Psychology ◽

10.1177/1359105320985580 ◽

2021 ◽

pp. 135910532098558

Author(s):

Carmina Castellano-Tejedor ◽

María Torres-Serrano ◽

Andrés Cencerrado

Keyword(s):

Chronic Conditions ◽

Population Survey ◽

Psychological Impact ◽

Survey Study ◽

Psychological Interventions ◽

Community Based ◽

Cross Sectional ◽

Young Females ◽

The World ◽

The Impact

The transformation that COVID-19 has brought upon the world is unparalleled. The impact on mental health is equally unprecedented and yet unexplored in depth. An online-based survey was administered to 413 community-based adults during COVID-19 confinement to explore psychological impact and identify high risk profiles. Young females concerned about the future, expressing high COVID-related distress, already following psychological therapy and suffering from pre-existing chronic conditions, were those at highest risk of psychological impact due to the COVID-19 situation. Findings could be employed to design tailored psychological interventions in the early stages of the outbreak to avoid the onset/exacerbation of psychopathology.

Download Full-text

The impact of the COVID-19 pandemic on socio-economic and sustainability

Environmental Science and Pollution Research ◽

10.1007/s11356-021-14986-0 ◽

2021 ◽

Author(s):

Xueli Wei ◽

Lijing Li ◽

Fan Zhang

Keyword(s):

World Economy ◽

Social Economy ◽

Secondary Data ◽

Well Being ◽

Cross Sectional ◽

The Social ◽

The World ◽

The Status ◽

Sectional Analysis ◽

The Impact

Pumping elephantThe COVID-19 pandemic has adversely affected the lives of people around the world in millions of ways . Due to this severe epidemic, all countries in the world have been affected by all aspects, mainly economic. It is widely discussed that the COVID-19 outbreak has affected the world economy. When considering this dimension, this study aims to examine the impact of the COVID-19 pandemic on the world economy, socio-economics, and sustainability. In addition, the research focuses on multiple aspects of social well-being during the pandemic, such as employment, poverty, the status of women, food security, and global trade. To this end, the study used time series and cross-sectional analysis of the data. The second-hand data used in this study comes from the websites of major international organizations. From the analysis of secondary data, the conclusion of this article is that the impact of the pandemic is huge. The main finding of the thesis is that the social economy is affected by the pandemic, causing huge losses in terms of economic well-being and social capital.

Download Full-text