Acute Granulocytic Ehrlichiosis in a Rottweiler

Acute granulocytic ehrlichiosis was identified in a 6-year-old rottweiler that was presented for possible pancreatitis. Intracytoplasmic inclusion bodies were identified within neutrophils on a peripheral blood smear. Serology was ineffective in identifying the disease in the acute state. The diagnosis and identification of the organism were confirmed by polymerase chain reaction (PCR) and deoxyribonucleic acid (DNA) sequencing. Based on elevations in amylase and lipase and the presence of right cranial-quadrant abdominal pain, concurrent pancreatitis was diagnosed. It is unknown if there was any association between the acute granulocytic ehrlichiosis and the pancreatitis. The dog recovered well following doxycycline therapy.

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Vivax malaria presenting with cerebral malaria and convulsions

Acta Parasitologica ◽

10.2478/s11686-010-0013-7 ◽

2010 ◽

Vol 55 (1) ◽

Cited By ~ 4

Author(s):

Anupkumar Anvikar ◽

Dinesh Singh ◽

Ruchi Singh ◽

Aditya Dash ◽

Neena Valecha

Keyword(s):

Polymerase Chain Reaction ◽

Platelet Count ◽

Vivax Malaria ◽

Laboratory Tests ◽

Enteric Fever ◽

Blood Smear ◽

Peripheral Blood Smear ◽

Chain Reaction ◽

Low Platelet Count ◽

Polymerase Chain

AbstractA patient was admitted with fever, vomiting, restlessness and convulsions. He was febrile and unconscious. Laboratory tests showed a low platelet count and ruled out enteric fever and dengue. His peripheral blood smear was positive for Plasmodium vivax. The presence of P. vivax monoinfection was confirmed by polymerase chain reaction and DNA sequencing. The report highlights the importance of considering the possibility of complications even in P. vivax malaria and formulation of strategies accordingly.

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Leishmaniasis Panamensis Masquerading as Myiasis and Sporotrichosis: A Clinical Pitfall

Case Reports in Pathology ◽

10.1155/2015/949670 ◽

2015 ◽

Vol 2015 ◽

pp. 1-3 ◽

Cited By ~ 1

Author(s):

Peter G. Pavlidakey ◽

Thy Huynh ◽

Kristopher Michael McKay ◽

Naveed Sami

Keyword(s):

Polymerase Chain Reaction ◽

Clinical Picture ◽

Blood Smear ◽

Peripheral Blood Smear ◽

Giemsa Stain ◽

Sodium Stibogluconate ◽

Chain Reaction ◽

Upper Arm ◽

Central Ulceration ◽

Polymerase Chain

We report a case of cutaneous leishmaniasis panamensis in nonendemic Costa Rica. A 19-year-old female presented with nonhealing, unilateral eruption of erythematous papules with superficial central ulceration in a sporotrichoid pattern on right upper arm and back. Given the clinical picture and geographic locale, the patient was initially diagnosed with myiasis or human botfly infestation; however, the sporotrichoid pattern of the bites is an unlikely finding in myiasis. Peripheral blood smear, Giemsa stain, and polymerase chain reaction (PCR) were consistent forLeishmaniaspp. Ulceration resolved with 20-day course of IV sodium stibogluconate.

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PCR - based diagnosis to evaluate the performance of malaria reference centers

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46652004000400002 ◽

2004 ◽

Vol 46 (4) ◽

pp. 183-187 ◽

Cited By ~ 15

Author(s):

Silvia Maria Di Santi ◽

Karin Kirchgatter ◽

Karen Cristina Sant'Anna Brunialti ◽

Alessandra Mota Oliveira ◽

Sergio Roberto Santos Ferreira ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Gold Standard ◽

Blood Smear ◽

Plasmodium Species ◽

Thick Blood Smear ◽

Chain Reaction ◽

Polymerase Chain ◽

Positive Results ◽

Ssrrna Gene

Although the Giemsa-stained thick blood smear (GTS) remains the gold standard for the diagnosis of malaria, molecular methods are more sensitive and specific to detect parasites and can be used at reference centers to evaluate the performance of microscopy. The description of the Plasmodium falciparum, P. vivax, P. malariae and P. ovale ssrRNA gene sequences allowed the development of a polymerase chain reaction (PCR) that had been used to differentiate the four species. The objective of this study was to determine Plasmodium species through PCR in 190 positive smears from patients in order to verify the quality of diagnosis at SUCEN's Malaria Laboratory. Considering only the 131 positive results in both techniques, GTS detected 4.6% of mixed and 3.1% of P. malariae infections whereas PCR identified 19.1% and 13.8%, respectively.

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Human papillomavirus deoxyribonucleic acid may not be detected in non-genital benign papillomatous skin lesions by polymerase chain reaction

Indian Journal of Dermatology ◽

10.4103/0019-5154.135475 ◽

2014 ◽

Vol 59 (4) ◽

pp. 334 ◽

Cited By ~ 2

Author(s):

Davoodi Kaveh ◽

Ayatollahi Hossein ◽

Ghanadan Alireza ◽

Damavandi Maede ◽

Aghazadeh Nessa ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Human Papillomavirus ◽

Deoxyribonucleic Acid ◽

Skin Lesions ◽

Chain Reaction ◽

Polymerase Chain

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Evaluation on the presence of Anaplasma, Ehrlichia, and Babesia spp. in goats (Capra hircus) in Cebu, the Philippines

Veterinary World ◽

10.14202/vetworld.2019.774-777 ◽

2019 ◽

Vol 12 (6) ◽

pp. 774-777 ◽

Cited By ~ 1

Author(s):

Adrian P. Ybañez ◽

Orgil V. Arrabis ◽

Dennis Justin M. Alvarez ◽

Eloiza May S. Galon ◽

Rhea Mae P. Jayag ◽

...

Keyword(s):

Blood Smear ◽

Peripheral Blood Smear ◽

The Philippines ◽

Capra Hircus ◽

Chain Reaction ◽

Nested Polymerase Chain Reaction ◽

Blood Samples ◽

Blood Smear Examination ◽

Polymerase Chain ◽

Babesia Spp

Background: Tick-borne diseases are caused by a wide variety of viruses, pathogens, and diseases. Anaplasma, Ehrlichia, and Babesia spp. are among the most known tick-borne pathogens in Asia. In the Philippines, these pathogens were already reportedly present in dogs and large ruminants, but no study has been reported yet evaluating their presence in goats. Aim: The present study aimed to evaluate the presence of Anaplasma, Ehrlichia, and Babesia spp. in goats in Cebu, the Philippines. Materials and Methods: A total of 100 blood samples from goats were collected in Cebu, the Philippines. Profile of sampled goats including age, body score, and sex was obtained. Peripheral blood smear examination and DNA extraction were performed. Nested polymerase chain reaction assay was used to evaluate the presence of Anaplasma, Ehrlichia, and Babesia spp. Results: None of the samples were found positive with Anaplasma, Ehrlichia, and Babesia spp. infection. Conclusion: Tested goats were negative with Anaplasma, Ehrlichia, and Babesia spp. and calls for continuous surveillance of these pathogens due to the reported detection of these pathogens in other livestock animals in the area.

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