Early diagnosis of familial nephropathy in English cocker spaniels

1998 ◽  
Vol 34 (3) ◽  
pp. 189-195 ◽  
Author(s):  
GE Lees ◽  
RG Helman ◽  
LD Homco ◽  
NJ Millichamp ◽  
JF Hunter ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Needle Biopsy ◽  
Definitive Diagnosis ◽  
Percutaneous Needle Biopsy ◽  
Biopsy Specimens ◽  
Transmission Electron ◽  
Renal Biopsies ◽  
Familial Nephropathy ◽  
Early Features ◽  
Microscopic Findings

Two litters of English cocker spaniels (ECSs) produced by familial nephropathy (FN) carriers were evaluated to characterize the early features of this disease. Three puppies developed FN. Proteinuria, which began when these puppies were five-to-eight months old, was the first abnormality detected. Proteinuria persisted while each puppy's growth rate slowed, and renal function gradually deteriorated. The interval from onset of proteinuria to development of azotemia was two-to-nine months. Characteristic glomerular capillary basement membrane (GCBM) lesions were seen with transmission electron microscopy (TEM) of renal biopsy specimens obtained during this interval. Ultrastructural GCBM lesions progressed substantially during the interval from biopsy to necropsy. However, routine light microscopic findings did not allow definitive diagnosis of FN in either biopsy or necropsy specimens. Detection of FN can be accomplished by screening at-risk ECSs for proteinuria. Renal biopsies are required to confirm the diagnosis in dogs for which proteinuria cannot be explained otherwise. Percutaneous needle biopsy specimens sufficient for TEM must be used to examine the GCBM to make a definitive diagnosis.

RENAL BIOPSY IN CHILDREN

PEDIATRICS ◽  
1958 ◽  
Vol 22 (6) ◽  
pp. 1033-1034
Author(s):  
ROBERT L. VERNIER ◽  
ROBERT A. GOOD
Keyword(s):  
Kidney Disease ◽  
Renal Biopsy ◽  
Needle Biopsy ◽  
Kidney Biopsy ◽  
Therapeutic Agents ◽  
Renal Diseases ◽  
Kidney Cortex ◽  
Percutaneous Needle Biopsy ◽  
Surgical Exploration ◽  
Renal Biopsies

RENAL biopsy offers invaluable aid in the clinical diagnosis of kidney disease and is an important technique in research designed to clarify the etiology, pathogenesis, and evaluation of therapeutic agents, in a variety of renal diseases. The majority of the scientific reports describing renal biopsy have concerned adult patients. The few available reports of renal biopsy in children do not discuss the risks attending the procedure or the specific problems peculiar to kidney biopsy in children. A review of our experience in 150 renal biopsies in children may afford a basis for evaluation of these questions. The available techniques of renal biopsy include: 1) surgical exploration and removal of a segment of kidney cortex, and 2) percutaneous needle biopsy.

scholarly journals The diagnosis of bilateral primary renal paragangliomas in a cat

2017 ◽  
Vol 88 ◽  
Author(s):  
Ryan B. Friedlein ◽  
Alain J. Carter ◽  
Robert D. Last ◽  
Sarah Clift
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Neuroendocrine Tumour ◽  
Tumour Progression ◽  
Serum Biochemistry ◽  
Needle Aspiration ◽  
Definitive Diagnosis ◽  
Abdominal Ultrasonography ◽  
Leukaemia Virus ◽  
Transmission Electron

A 9-year-old sterilised female domestic short-hair cat was referred with a history of vomiting and anorexia of 3 months’ duration. Biochemistry, full-blood counts, thoracic radiographs, feline pancreatic-specific lipase, abdominal ultrasonography and feline immunodeficiency virus/feline leukaemia virus (FIV/FeLV) SNAP tests had been performed. Mild hypochloraemia and moderate hypokalaemia were evident on initial presentation. Abdominal ultrasonography initially revealed unilateral renal nodules on the left side. These were subjected to fine-needle aspiration and cytological evaluation. A neuroendocrine tumour was suspected, and biopsies via midline coeliotomy were taken to confirm the diagnosis. Initial histopathology diagnosed primary renal carcinomas or neuroendocrine neoplasia; however, the definitive diagnosis became renal paragangliomas after immunohistochemistry and transmission electron microscopy were performed. The cat was regularly monitored with serum biochemistry parameters, blood pressure determinations, thoracic radiographs and subsequent abdominal ultrasonography. Biochemistry, radiography and blood pressures remained normal over a 24-week follow-up period, while subsequent ultrasonography revealed tumour progression in both number and size in both kidneys. Primary neuroendocrine tumours of the kidney are frequently incorrectly diagnosed as other renal tumours such as renal cell carcinoma, mesonephric tumours or undifferentiated carcinomas. This case report highlights the importance of additional testing, including immunohistochemistry and transmission electron microscopy, to obtain a definitive diagnosis of paragangliomas.

Estimation of Quartz in Small Biological Specimens with Transmission Electron Microscopy at 100 KV

1974 ◽  
Vol 32 ◽  
pp. 70-71
Author(s):  
Glenn R. Smith ◽  
Krishna Seshan ◽  
Jerome J. Wesolowski ◽  
Axel G. Berner
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Mass Concentration ◽  
Oxygen Plasma ◽  
Human Lung ◽  
X Ray Diffraction ◽  
X Ray ◽  
Biopsy Specimens ◽  
Transmission Electron

Determination of the mass concentration of quartz in small biopsy specimens for clinical diagnostic purposes often cannot be performed by x-ray diffraction and light microscopy. A technique utilizing transmission electron microscopy (TEM) was used to determine the quartz concentration in a human lung specimen taken from a patient suspected of having silicosis.The sample was prepared for examination by low temperature incineration in an oxygen plasma. The inorganic residue was suspended in 0.5 percent Parlodion in amyl acetate. Grids were coated with the sample suspension and subsequently shadowed with a thin film of carbon. Standard quartz particles were prepared in a similar manner.

Fluorescence in situ hybridization (FISH) and array-comparative genomic hybridization (a-CGH) from percutaneous needle biopsy compared to renal mass histology.

2013 ◽  
Vol 31 (6_suppl) ◽  
pp. 471-471
Author(s):  
Massimiliano Spaliviero ◽  
Kelly Lynn Stratton ◽  
Timothy F. Donahue ◽  
Banumathy Gowrishankar ◽  
Charles Ma ◽  
...  
Keyword(s):  
Needle Biopsy ◽  
Renal Mass ◽  
Renal Tumors ◽  
Comparative Genomic ◽  
Initial Experience ◽  
Percutaneous Needle Biopsy ◽  
Renal Masses ◽  
Surgical Specimen ◽  
Subtype Classification ◽  
Biopsy Specimens

471 Background: Image-guided percutaneous needle biopsies are increasingly utilized for the diagnosis of renal tumors. Histologic diagnosis of renal mass subtypes, including malignant clear cell (ccRCC), papillary (pRCC), chromophobe (chrRCC) renal cell carcinoma, and benign oncocytomas (OC) can be challenging due to the low cellularity and damaged architecture of needle biopsy specimens. However, each subtype exhibits unique genetic aberrations that can assist in histologic classification and potentially assist in guiding management decisions. We report our initial experience correlating renal mass histology to subtype-associated genomic alterations detected by FISH and a-CGH of percutaneous needle biopsy specimens. Methods: In an ongoing IRB-approved blinded study, 17 samples obtained from 15 patients with known renal masses were submitted to FISH (FReCaD) and targeted a-CGH (UroGenRA-Kidney). Specimens were classified using a subtype classification decision tree algorithm based on the presence/absence of genomic abnormalities. The results were compared to biopsy histology or surgical specimen when available. Results: Histology revealed ccRCC in 6 patients, pRCC in 4, OC in 2, Angiomyolipoma in 1, and unclassified type RCC in 2. In 6 of 9 cases FISH achieved a diagnosis, which correlated with histology in 4. FISH incorrectly classified as ccRCC two cases with pRCC on histology. A-CGH was diagnostic in 14 of 15 cases and correlated with histology in 13. In one case, a-CGH showed a genomic profile not consistent with ccRCC, pRCC, chrRCC, or OC according to the algorithm. Conclusions: The addition of genetic tumor tissue studies to complement histology from biopsy tissues may supplement or improve the accuracy of classification and biological characterization of renal tumor biopsies and influence treatment planning. In our initial experience, a-CGH showed better correlation with histology in subtype classification of malignant and benign renal masses than FISH. Prospective testing will be required to validate these results.

scholarly journals Variability in Hepatic Iron Concentration in Percutaneous Needle Biopsy Specimens From Patients With Transfusional Hemosiderosis

2005 ◽  
Vol 123 (1) ◽  
pp. 146-152 ◽  
Author(s):  
Ellen Butensky ◽  
Roland Fischer ◽  
Mark Hudes ◽  
Laurie Schumacher ◽  
Roger Williams ◽  
...  
Keyword(s):  
Needle Biopsy ◽  
Iron Concentration ◽  
Hepatic Iron ◽  
Percutaneous Needle Biopsy ◽  
Biopsy Specimens ◽  
Hepatic Iron Concentration

Electron microscopy of ultrasound-guided fine-needle biopsy specimens

1987 ◽  
Vol 60 (712) ◽  
pp. 351-353 ◽  
Author(s):  
Maxwell Sehested ◽  
Niels Juul ◽  
Bo Hainau ◽  
Søren Torp-Pedersen
Keyword(s):  
Electron Microscopy ◽  
Needle Biopsy ◽  
Fine Needle Biopsy ◽  
Ultrasound Guided ◽  
Fine Needle ◽  
Biopsy Specimens

Ultrastructural Studies of Acute Rheumatic Valvulitis

1977 ◽  
Vol 35 ◽  
pp. 518-519
Author(s):  
S. Siew
Keyword(s):  
Electron Microscopy ◽  
Aortic Insufficiency ◽  
Elastic Tissue ◽  
Chordae Tendineae ◽  
Valve Mechanism ◽  
Electron Microscopic ◽  
Ultrastructural Studies ◽  
Transmission Electron ◽  
Clinical Diagnoses ◽  
Microscopic Findings

The advent of cardiac surgery has accorded the opportunity of obtaining fresh human tissue suitable for electron microscopy. The percentage of acute rheumatic lesions in such material is understandably small and there has been comparatively little progress with the ultrastructural investigation of this stage of the disease. This report presents the transmission and scanning electron microscopic findings of 3 cardiac valves: a mitral, a tricuspid and an aortic, which had been resected on the basis of the clinical diagnoses of mitral stenosis, tricuspid stenosis and aortic insufficiency, respectively. Gross and histological examination showed the classical features of acute and chronic rheumatic valvulitis.Transmission electron microscopy was performed on the valves and on the related structures of the atrio-ventricu1ar valve mechanism, the chordae tendineae and the papillary muscles. The valves were thickened and showed the presence of abundant collagen, some elastic tissue and foci of calcification.

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