scholarly journals PADRONIZAÇÃO DA CITOLOGIA DE IMPRESSÃO DA SUPERFÍCIE OCULAR CANINA

2005 ◽  
Vol 10 (1) ◽  
Author(s):  
C.A.L. GODOY-ESTEVES ◽  
J.N. BARROS ◽  
L.S. CUNHA ◽  
V.L.D. MASCARO ◽  
A.L. HOFLING-LIMA ◽  
...  
Keyword(s):  
Filter Paper ◽  
Ocular Surface ◽  
Goblet Cells ◽  
Eye Disease ◽  
Conjunctival Epithelium ◽  
Human Beings ◽  
Impression Cytology ◽  
Feasible Method ◽  
Veterinary Hospital ◽  
Goblet Cell Density

Técnica de exame de citologia de impressão foi padronizada em olhos de cães sem alterações oculares. Foram realizados exames de citologia de impressão do epitélio corneano, conjuntival e tarsal em 30 olhos de 21 animais de raças e idades variadas. As amostras foram colhidas de cães atendidos no Hospital Veterinário da FMVZ-USP entre fevereiro e julho de 2003, sendo coradas e avaliadas no Laboratório de Doenças Externas Oculares da UNIFESP. A colheita foi bem tolerada pelos cães e o papel filtro utilizado removeu células em quantidade e morfologia adequadas para estudo citológico. Foi observado em 100% dos casos que o epitélio da conjuntiva bulbar canina apresenta aspecto “metaplasia-like”, com ausência de células caliciformes. Estas só foram encontradas na conjuntiva tarsal em 21,4% das amostras avaliadas dessa região. A citologia de impressão é um método factível para avaliação da superfície ocular em cães. Entretanto, a celularidade das amostras obtidas do tarso mostrou-se inadequada. Além disso, a pesquisa da densidade de células caliciformes em áreas bulbares, embora usada em seres humanos, pode não servir como indicador de alteração da superfície ocular para a espécie canina. Standardization of canine ocular surface impression cytology Abstract Impression cytology technique in dog eyes without ocular disease was standardized. Impression cytology was performed in corneal, conjunctival and tarsal epithelium in 30 eyes of 21 animals with different races and ages. Samples were obtained from dogs attended in FMVZ-USP Veterinary Hospital between February to July 2003, being stained and evaluated at UNIFESP´s External Eye Disease Laboratory. Sampling was well tolerated by dogs and the filter paper used removed cells with adequate morphology and quantity for cytologyc evaluation. In all cases canine bulbar conjunctival epithelium showed metaplasia-like features without goblet cells. Impression cytology is a feasible method for ocular surface evaluation in dogs. However, celularity was considered inadequated in samples obtained from tarsal conjunctiva. Furthermore, seeking goblet cell density in bulbar areas, although used in human beings, may not be used as an ocular surface disease indicator in canine species.

scholarly journals Tear Ferning Test and Pathological Effects on Ocular Surface before and after Topical Cyclosporine in Vernal Keratoconjunctivitis Patients

2018 ◽  
Vol 2018 ◽  
pp. 1-11
Author(s):  
Marcella Nebbioso ◽  
Marta Sacchetti ◽  
Guia Bianchi ◽  
Anna Maria Zicari ◽  
Marzia Duse ◽  
...  
Keyword(s):  
Cell Density ◽  
Goblet Cell ◽  
Ocular Surface ◽  
Goblet Cells ◽  
Significant Alteration ◽  
Vernal Keratoconjunctivitis ◽  
Eye Drops ◽  
Before And After ◽  
Tear Ferning Test ◽  
Goblet Cell Density

Background. Vernal keratoconjunctivitis (VKC) is a rare ocular surface inflammatory disease that affects mainly boys in the first decade of life. Clinical observations show that it generally regresses spontaneously with the onset of puberty, but therapeutic measures must be taken before then to control the course of the disease. Purpose.To evaluate the role of the lacrimal mucous component in VKC patients and compare tear ferning test (TFT) modifications, MUC5AC levels in tears, and density of conjunctival goblet cells to clinical characteristics before and after treatment with cyclosporine A (CY) in eye drops. Methods. Forty-seven patients affected by VKC and 30 healthy subjects aged between 3 and 16 years of life were enrolled. All individuals were submitted to complete eye examination and skin prick test (SPT) for the most common allergens. Then, they were subjected to collection of the tears and to impression cytology to evaluate TFT, MUC5AC levels, and conjunctival goblet cell density, before and after treatment with CY in eye drops. Results. Comparing the VKC group vs. the control group at baseline, a significant alteration in the degree of the ferns was found, indicating a pathological condition of the lacrimal mucous layer. In addition, an increased number of goblet cells were observed in the patients. The concentration of lacrimal secretory mucins (MUC5AC) did not show significant differences between the 2 groups. Patients treated with CY have reported improvements of some signs and symptoms of disease activity, including TFT, and a tendency of conjunctival goblet cell density to normalise. Conclusions. The results obtained demonstrated for the first time a significant alteration of the lacrimal mucin component evaluated in the VKC group, and an improvement of the latter after CY therapy.

scholarly journals Toxicity of Povidone-Iodine to the Ocular Surface of Rabbits

2019 ◽  
Author(s):  
Sun Young Kim ◽  
Yong Sun Ahn ◽  
Yeo Jin Lee ◽  
Hyun Seung Kim
Keyword(s):  
Ocular Surface ◽  
Povidone Iodine ◽  
Study Groups ◽  
Ultrastructural Changes ◽  
Control Group ◽  
Dependent Manner ◽  
Impression Cytology ◽  
Schirmer Test ◽  
Phosphate Buffered Saline ◽  
Goblet Cell Density

Abstract Background We evaluated the toxicity of 5% (w/v) povidone-iodine (PI) applied to the ocular surface of rabbits. Methods Twenty three white rabbits were divided into four groups; these were a control group and three study groups in which the ocular surface was exposed to PI for different times. In control group, phosphate-buffered saline (PBS) was applied once for 10 minutes. In study groups, 5% (w/v) PI was topically applied once for 1 minute, 3 minutes, and 10 minutes, and then the animals were observed for 7 days. The Schirmer test, Rose Bengal staining, corneal fluorescein staining and conjunctival impression cytology were performed on day 0, 3, and 7. After 7 days, the rabbits were sacrificed and conjunctiva and cornea were collected and evaluated by light and electron microscope. Immunofluorescence staining was also performed to detect mucin 5 subtype AC (MUC5AC). Results The decrease in goblet cell density, reductions in MUC5AC level and histopathological and ultrastructural changes of conjunctiva and cornea were more prominent in the 5% (w/v) PI groups than the control group (p < 0.05). Moreover, these changes were more prominent when PI was applied for 3 and 10 minutes rather than 1 minute (both p values < 0.05). Conclusions 5% (w/v) povidone-iodine caused damages to the ocular surface in a time-dependent manner. Therefore, we should be aware of that excessive PI exposure during ophthalmic procedures could be a pathogenic factor of dry eye syndrome after surgery.

IMPRESSIONABLE CYTOLOGY METHOD IN DIAGNOSTICS OF DRY EYE SYNDROME

2019 ◽  
Vol 64 (6) ◽  
pp. 348-350
Author(s):  
N. M. Zakharova ◽  
I. P. Shabalova ◽  
G. H. Ali-Zade ◽  
A. A. Rjabtseva ◽  
O. N. Vetchinnikova ◽  
...  
Keyword(s):  
Dry Eye ◽  
Squamous Epithelium ◽  
Goblet Cells ◽  
Dry Eye Syndrome ◽  
Eye Disease ◽  
Control Group ◽  
Impression Cytology ◽  
Cytological Examination ◽  
Main Component ◽  
Cytological Sample

Analysis of the cellular composition of the eyeball conjunctival prints and evaluation of the informative of the cytomorphological method of research in the diagnosis of dry eye syndrome (DES). Cytological examination using impression cytology was performed in 72 patients: 60 patients with DES and 12 without eye disease (control group). The main component of cytological sample in patients with DES are (a) squamous epithelium (hyperkeratocytes); (b) groups and clusters of flattened epithelial cells; (c) a small amount (up to five in the preparation) of goblet cells. Impression cytology method improves the accuracy of diagnosis of DES.

The analysis of human conjunctival epithelium proteome in ocular surface diseases using impression cytology and 2D-DIGE

2018 ◽  
Vol 167 ◽  
pp. 31-43 ◽  
Author(s):  
Javier Soria ◽  
Arantxa Acera ◽  
Juan A. Durán ◽  
Ana Boto-de-los-Bueis ◽  
Almudena Del-Hierro-Zarzuelo ◽  
...  
Keyword(s):  
Ocular Surface ◽  
Conjunctival Epithelium ◽  
Impression Cytology ◽  
2D Dige ◽  
Ocular Surface Diseases

FTY720 ameliorates Dry Eye Disease in NOD mice: Involvement of leukocytes inhibition and goblet cells regeneration in ocular surface tissue

2015 ◽  
Vol 138 ◽  
pp. 145-152 ◽  
Author(s):  
Weibao Xiao ◽  
Guo-Tong Xu ◽  
Jingfa Zhang ◽  
Jiaying Zhang ◽  
Yu Zhang ◽  
...  
Keyword(s):  
Dry Eye ◽  
Ocular Surface ◽  
Nod Mice ◽  
Goblet Cells ◽  
Dry Eye Disease ◽  
Eye Disease

scholarly journals Conjunctival Goblet Cell Responses to TLR5 Engagement Promote Activation of Local Antigen-Presenting Cells

2021 ◽  
Vol 12 ◽  
Author(s):  
Abiramy Logeswaran ◽  
Laura Contreras-Ruiz ◽  
Sharmila Masli
Keyword(s):  
Dendritic Cells ◽  
Goblet Cell ◽  
Ocular Surface ◽  
Primary Cultures ◽  
Goblet Cells ◽  
Microbial Colonization ◽  
Mucosal Inflammation ◽  
Conjunctival Epithelium ◽  
Cell Responses ◽  
Mucosal Homeostasis

Conjunctival epithelium forms a barrier between the ocular surface microbial flora and the ocular mucosa. In addition to secreting gel-forming mucins, goblet cells, located in the conjunctival epithelium, help maintain local immune homeostasis by secreting active TGFβ2 and promoting tolerogenic phenotype of dendritic cells in the vicinity. Although dendritic cell subsets, characteristic of mucosal tissues, are found in the conjunctiva, previous studies provided limited information about their location within the tissue. In this study, we examine immunostained conjunctiva explants to determine the location of CD11c-positive dendritic cells in the context of MUC5AC-positive goblet cells. Considering that conjunctival goblet cells are responsive to signaling induced by pathogen recognition receptors, we also assess if their responses to microbial product, flagellin, can contribute to the disruption of ocular mucosal homeostasis that promotes activation of dendritic cells and results in chronic ocular surface inflammation. We find that dendritic cells in the conjunctiva with an increased microbial colonization are located adjacent to goblet cells. While their cell bodies in the stromal layer are immediately below the epithelial layer, several extensions of dendritic cells are projected across the epithelium towards the ocular surface. Such trans-epithelial dendrites are not detectable in healthy ocular mucosa. In response to topically applied flagellin, increased proportion of CD11c-positive cells in the conjunctiva strongly express MHC class II relative to the untreated conjunctiva. This change is accompanied by reduced immunoreactivity to TGFβ-activating Thrombospondin-1 in the conjunctival epithelium. These findings are supported by in vitro observations in primary cultures of goblet cells that respond to the TLR5 stimulation with an increased expression of IL-6 and reduced level of active TGFβ. The observed changes in the conjunctiva after flagellin application correspond with the development of clinical signs of chronic ocular mucosal inflammation including corneal epitheliopathy. Collectively, these findings demonstrate the ability of ocular mucosal dendritic cells to extend trans-epithelial dendrites in response to increased microbial colonization at the ocular surface. Moreover, this study provides key insight into how goblet cell responses to microbial stimuli may contribute to the disruption of ocular mucosal homeostasis and chronic ocular mucosal inflammation.

scholarly journals Toxicity of Povidone-Iodine to the Ocular Surface of Rabbits

2020 ◽  
Author(s):  
Sun Young Kim ◽  
Yong Sun Ahn ◽  
Yeo Jin Lee ◽  
Hyun Seung Kim
Keyword(s):  
Ocular Surface ◽  
Povidone Iodine ◽  
Study Groups ◽  
Ultrastructural Changes ◽  
Control Group ◽  
Dependent Manner ◽  
Impression Cytology ◽  
Schirmer Test ◽  
Phosphate Buffered Saline ◽  
Goblet Cell Density

Abstract Background : We evaluated the toxicity of 5% (w/v) povidone-iodine (PI) applied to the ocular surface of rabbits. Methods: Twenty-three white rabbits were divided into four groups; these were a control group and three study groups in which the ocular surface was exposed to PI for different times. In control group, one drop of phosphate-buffered saline (PBS) was applied once for 10 minutes. In study groups, one drop of 5% (w/v) PI was topically applied once for 1 minute, 3 minutes, and 10 minutes, and then the animals were observed for 7 days. The Schirmer test, Rose Bengal staining, corneal fluorescein staining and conjunctival impression cytology were performed on day 0, 3, and 7. After 7 days, the rabbits were sacrificed and conjunctiva and cornea were collected and evaluated by light and electron microscope. Immunofluorescence staining was also performed to detect mucin 5 subtype AC (MUC5AC). Results: The decrease in goblet cell density, reductions in MUC5AC level and histopathological and ultrastructural changes of conjunctiva and cornea were more prominent in the 5% (w/v) PI groups than the control group ( p < 0.05). Moreover, these changes were more prominent when PI was applied for 3 and 10 minutes rather than 1 minute (both p values < 0.05). Conclusions: 5% (w/v) povidone-iodine caused damages to the ocular surface in a time-dependent manner. Therefore, we should be aware of that excessive PI exposure during ophthalmic procedures could be a pathogenic factor of dry eye syndrome after surgery.

Impression Cytology on Conjunctiva and Cornea in Dry Eye Patients Establishes a Correlation between Squamous Metaplasia and Dry Eye Clinical Severity

2003 ◽  
Vol 13 (2) ◽  
pp. 115-127 ◽  
Author(s):  
J. Murube ◽  
L. Rivas
Keyword(s):  
Epithelial Cells ◽  
Cell Size ◽  
Dry Eye ◽  
Ocular Surface ◽  
Squamous Metaplasia ◽  
Goblet Cells ◽  
Clinical Severity ◽  
Grading System ◽  
Impression Cytology ◽  
Nuclear Alterations

Purpose To obtain deeper knowledge of the cellular transition in squamous metaplasia, and to look for a correlation between the clinical grade of severity of dry eye and the grade of squamous metaplasia of the corneal and conjunctival epithelium, studied by impression cytology. Methods A total of 143 patients with dry eye disorders of different grades of clinical severity and 33 control subjects of matched age and sex were studied. Symptoms, clinical tests (including Schirmer test, slit-lamp examination, break-up time, rose Bengal staining, vanishing lacunar sulci, and neovascularization), and tear osmolarity were used to establish the diagnosis of dry eye. The subjects were classified into six clinical grades, grade 0 indicating normal and grades 1 to 5 progressively more severe dry eye. Impression cytology specimens were taken from the central cornea and different areas of the conjunctiva of one eye from all patients. A morphologic and morphometric study of the photographs obtained by light microscopy showed cell size, nuclear size, nuclear-cytoplasmic ratio (N:C) in nonsecretory epithelial cells, and density of goblet cells. Results Morphometric and morphologic studies of the ocular surface cells indicated significant differences, mainly in cell sizes, nuclear alterations, and the N:C ratio, in nonsecretory epithelial cells of the conjunctiva and cornea, and in goblet cell densities from the conjunctiva, between the clinically normal eyes and those with the five grades of clinical severity of dry eye, with different degrees of squamous metaplasia. Conclusions A morphologic and morphometric analysis of the ocular surface from patients with dry eye obtained by impression cytology led us to draft a new grading system containing one normal level and five levels of squamous metaplasia. This new grading system is based on a significant decrease in the number of goblet cells with less periodic acid-Schiff-hematoxylin-positive staining, an increase in nonsecretory cell size, more marked cell separation, a lower N:C ratio, and an increase in nuclear alterations. The clinical severity of the dry eye correlates with these alterations.

scholarly journals Toxicity of Povidone-Iodine to the Ocular Surface of Rabbits

2020 ◽  
Author(s):  
Sun Young Kim ◽  
Yong Sun Ahn ◽  
Yeo Jin Lee ◽  
Hyun Seung Kim
Keyword(s):  
Ocular Surface ◽  
Povidone Iodine ◽  
Study Groups ◽  
Ultrastructural Changes ◽  
Control Group ◽  
Dependent Manner ◽  
Impression Cytology ◽  
Schirmer Test ◽  
Phosphate Buffered Saline ◽  
Goblet Cell Density

Abstract Background: We evaluated the toxicity of 5% (w/v) povidone-iodine (PI) applied to the ocular surface of rabbits.Methods: Twenty-three white rabbits were divided into four groups; these were a control group and three study groups in which the ocular surface was exposed to PI for different times. In control group, one drop of phosphate-buffered saline (PBS) was applied once for 10 minutes. In study groups, one drop of 5% (w/v) PI was topically applied once for 1 minute, 3 minutes, and 10 minutes, and then the animals were observed for 7 days. The Schirmer test, Rose Bengal staining, corneal fluorescein staining and conjunctival impression cytology were performed on day 0, 3, and 7. After 7 days, the rabbits were sacrificed and conjunctiva and cornea were collected and evaluated by light and electron microscope. Immunofluorescence staining was also performed to detect mucin 5 subtype AC (MUC5AC).Results: The decrease in goblet cell density, reductions in MUC5AC level and histopathological and ultrastructural changes of conjunctiva and cornea were more prominent in the 5% (w/v) PI groups than the control group (p < 0.05). Moreover, these changes were more prominent when PI was applied for 3 and 10 minutes rather than 1 minute (both p values < 0.05).Conclusions: 5% (w/v) povidone-iodine caused damages to the ocular surface in a time-dependent manner. Therefore, we should be aware of that excessive PI exposure during ophthalmic procedures could be a pathogenic factor of dry eye syndrome after surgery.

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