scholarly journals Tear Ferning Test and Pathological Effects on Ocular Surface before and after Topical Cyclosporine in Vernal Keratoconjunctivitis Patients

2018 ◽  
Vol 2018 ◽  
pp. 1-11
Author(s):  
Marcella Nebbioso ◽  
Marta Sacchetti ◽  
Guia Bianchi ◽  
Anna Maria Zicari ◽  
Marzia Duse ◽  
...  
Keyword(s):  
Cell Density ◽  
Goblet Cell ◽  
Ocular Surface ◽  
Goblet Cells ◽  
Significant Alteration ◽  
Vernal Keratoconjunctivitis ◽  
Eye Drops ◽  
Before And After ◽  
Tear Ferning Test ◽  
Goblet Cell Density

Background. Vernal keratoconjunctivitis (VKC) is a rare ocular surface inflammatory disease that affects mainly boys in the first decade of life. Clinical observations show that it generally regresses spontaneously with the onset of puberty, but therapeutic measures must be taken before then to control the course of the disease. Purpose.To evaluate the role of the lacrimal mucous component in VKC patients and compare tear ferning test (TFT) modifications, MUC5AC levels in tears, and density of conjunctival goblet cells to clinical characteristics before and after treatment with cyclosporine A (CY) in eye drops. Methods. Forty-seven patients affected by VKC and 30 healthy subjects aged between 3 and 16 years of life were enrolled. All individuals were submitted to complete eye examination and skin prick test (SPT) for the most common allergens. Then, they were subjected to collection of the tears and to impression cytology to evaluate TFT, MUC5AC levels, and conjunctival goblet cell density, before and after treatment with CY in eye drops. Results. Comparing the VKC group vs. the control group at baseline, a significant alteration in the degree of the ferns was found, indicating a pathological condition of the lacrimal mucous layer. In addition, an increased number of goblet cells were observed in the patients. The concentration of lacrimal secretory mucins (MUC5AC) did not show significant differences between the 2 groups. Patients treated with CY have reported improvements of some signs and symptoms of disease activity, including TFT, and a tendency of conjunctival goblet cell density to normalise. Conclusions. The results obtained demonstrated for the first time a significant alteration of the lacrimal mucin component evaluated in the VKC group, and an improvement of the latter after CY therapy.

Ocular surface changes in recurrent pterygium cases post-operatively treated with 5-fluorouracil subconjunctival injections

2018 ◽  
Vol 29 (1) ◽  
pp. 9-14 ◽  
Author(s):  
Amanda García Tirado ◽  
Ana Boto de los Bueis ◽  
Luis Rivas Jara
Keyword(s):  
Cell Density ◽  
Goblet Cell ◽  
Ocular Surface ◽  
Squamous Metaplasia ◽  
Cell Number ◽  
Before And After ◽  
Intralesional Injections ◽  
Recurrent Pterygium ◽  
Goblet Cell Density ◽  
Surface Changes

Introduction: To investigate the ocular surface changes occurring in eyes with recurrent pterygium post-operatively treated with 5-fluorouracil intralesional injections. Methods: Retrospective observational study of recurrent pterygium cases treated with weekly intralesional injections of 0.1 mL (5 mg) of 5-fluorouracil (10 injections). Impression cytology samples taken from the lesion, the healthy conjunctivae (inferior, superior, and contralateral to injury), and the cornea before and after treatment were analyzed. Clinical ocular characteristics (including Schirmer’s test and break-up time) were evaluated during treatment. Results: A total of 15 eyes were treated, with the mean follow-up of 27 ± 8.7 months (mean ± standard deviation). Prior to treatment initiation, the ocular surface citology over the pterygium was found to be abnormal. No epithelial cells (27%) and a lower goblet cell density (73%) compared to the healthy conjunctivae (p < 0.01) were found. Squamous metaplasia was observed to some degree in the cornea (100%), pterygium (81%) and healthy conjunctivae (73%). Following treatment, pterygium composition had changed: epithelial cell number (100%) and goblet cell density (47%) had increased (p < 0.05). Goblet cell density was also increased in healthy conjunctivae (67%; p < 0.05). The degree of squamous metaplasia decreased in the cornea (67%), pterygium (45%), and healthy conjunctivae (60%; p < 0.05). No adverse effects were reported, recurrence progression was arrested, and conjunctival redness and dry-eye severity level were decreased in all cases (p < 0.01). Discussion: The cytology of ocular surface in recurrent pterygium is abnormal. After weekly intralesional 5-fluorouracil injections, it tends to normalize. The 5-fluorouracil compound is a safe and effective treatment to prevent pterygium recurrence.

Comparison of Ocular Surface Cytological Appearance in Glaucoma Patient Treated with Timolol Maleat 0,5% Latanoprost 0,005% and Timolol-Latanoprost Fixed Combination Preservative Free Eye Drop

2019 ◽  
Vol 44 (2) ◽  
pp. 82
Author(s):  
Maretha Amrayni ◽  
Elsa Gustianty ◽  
Susi Heryati ◽  
Andika Prahasta ◽  
Maula Rifada ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Cell Density ◽  
Goblet Cell ◽  
Ocular Surface ◽  
Fixed Combination ◽  
Cell Contact ◽  
Corneal Epithelial ◽  
Preservative Free ◽  
Goblet Cell Density ◽  
Epithelial Metaplasia

Introduction : The longterm use of topical antiglaucoma might cause ocular surface instability due to active substance or preservative used. Impression cytology examination may reveal superficial epithelial cells on conjunctiva and cornea, including goblet cells. Goblet cell density decrease is the most important parameter on evaluation of ocular surface disorder. Objective : This study was to understand ocular surface remodeling due to active substance of topical antiglaucoma with impression cytology examination among the patient prior and 3 months after therapy. Methods : This was a randomized controlled trial study with single blind masking. A total of 45 eyes from 31 patients were used as subject and distributed onto three groups treatment, which were timolol maleat 0.5%, latanoprost 0.005%, and latanoprost-timolol maleat fixed combination. All topical antiglaucoma in this study were preservative free. Result : There were differences between 3 groups in goblet cells density after 3 months therapy (p=0,030). Goblet cell density in timolol group was lower than latanoprost (p=0,041) and fixed combination (p=0,045). There was no significantly difference between 3 groups in conjunctival epithelial metaplasia degree (p=0,706) and cell to cell contact degree in corneal epithelial cells (p=0.66) after 3 months therapy. Conjunctival epithelial metaplasia degree were increased among group of timolol (p=0,008) and fixed combination (p=0,046). Conclusion : Timolol maleat 0,5% caused lower goblet cell density after 3 months therapy compare with latanoprost and fixed combination. There was no significantly difference in conjunctival epithelial metaplasia and cell to cell contact degree in corneal epithelial cells among these glaucoma treatment groups.

Distribution and Density of Goblet Cells in the Normal Adult Human Middle Ear

1973 ◽  
Vol 82 (2) ◽  
pp. 240-247 ◽  
Author(s):  
K. Bak-Pedersen ◽  
M. Tos
Keyword(s):  
Cell Density ◽  
Middle Ear ◽  
Goblet Cell ◽  
Anterior Part ◽  
Goblet Cells ◽  
Mastoid Process ◽  
Clinically Normal ◽  
Normal Series ◽  
Temporal Bones ◽  
Goblet Cell Density

The purpose of this study was to map the distribution and density of goblet cells in the clinically normal middle ear. From 12 temporal bones, derived from 12 adult patients with clinically normal middle ears, the mucosa was prepared and stained by the PAS-alcian blue whole-mount method. In six of the patients the middle ears with meticulous gross and microscopic investigation showed mild sequelae of previous otitis media, called the slightly abnormal series, whereas the other six patients who had entirely normal middle ears were designated the normal series. Each middle ear was investigated in 12 different areas: tympanic orifice, hypotympanum anteriorly and posteriorly, round window niche, oval window niche, promontory anteriorly, in the middle, and posteriorly, epitympanum, antrum, and mastoid process. In each middle ear area the goblet-cell density and distribution were determined on the basis of 30 to 50 counts of 0.01768 mm2 epithelial surface, a total of at least 4800 counts. In six patients goblet cells were found in all 12 areas, in three patients in 11, and in three patients in 10. In both series there was a distinct decrease in goblet-cell density from the tympanic orifice, towards the posterior areas of the middle ear and the mastoid process. However, two patients of the slightly abnormal series exhibited a considerably greater density in the posterior than in the anterior part of the hypotympanum. The mean goblet-cell density in the slightly abnormal series was distinctly greater in all middle ear areas than in the normal series. Correspondingly, goblet cells were demonstrated in the antrum and mastoid process in all patients of the slightly abnormal series, but in only half of the normal series. Therefore, the greater goblet-cell density in the slightly abnormal series presumaby results from a previous past disease condition of the middle ear.

Goblet Cell Population in the Pathological Middle Ear and Eustachian Tube of Children and Adults

1977 ◽  
Vol 86 (2) ◽  
pp. 209-218 ◽  
Author(s):  
Mirko Tos ◽  
K. Bak-Pedersen
Keyword(s):  
Cell Density ◽  
Eustachian Tube ◽  
Middle Ear ◽  
Goblet Cell ◽  
Newborn Infants ◽  
Goblet Cells ◽  
Secretory Otitis Media ◽  
Temporal Bones ◽  
Different Parts ◽  
Goblet Cell Density

On 64 temporal bones from slightly abnormal and pathological prematures and newborn infants, children, and adults, 35,000–40,000 counts of goblet cells were done in different parts of the Eustachian tube and middle ear. The goblet cells were present in all Eustachian tubes and middle ear areas. In ears previously exposed to infection the density was slightly increased in the tubal orifice and in most parts of the middle ear. In subacute and acute pathological actions, especially tubal occlusion, and in chronic diseases, such as active chronic otitis and in secretory otitis media, the goblet-cell density is appreciably increased.

Scanning Electron Microscopy of Experimental Keratoconjunctivitis Sicca in Dogs: Cornea and Bulbar Conjunctiva

1988 ◽  
Vol 25 (6) ◽  
pp. 468-474 ◽  
Author(s):  
T. J. Kern ◽  
H. N. Erb ◽  
J. M. Schaedler ◽  
E. P. Dougherty
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Replacement Therapy ◽  
Cell Density ◽  
Goblet Cell ◽  
Keratoconjunctivitis Sicca ◽  
Goblet Cells ◽  
Dark Cell ◽  
Scanning Electron ◽  
Goblet Cell Density

Keratoconjunctivitis sicca was produced experimentally in 16 beagles by bilateral surgical removal of the lacrimal and nictitans glands; four dogs were not treated, and 12 received tear-replacement therapy on post-operative days 7 through 28. Keratoconjunctivitis sicca was verified by reduction in Schirmer tear test values by post-operative day 6, and there was no response on day 28 to tear-replacement therapy. Corneas of both normal and tear-deficient dogs had polygonal squamous epithelial cells of light and dark electron density by scanning electron microscopy. Light cells had more microvilli and microplicae than dark cells. Conjunctivae were similar to corneas, except for numerous goblet cells on the surface. Corneal dark-cell density and goblet cell density were not different between groups. Goblet cells most often occurred singly in normal dogs, while they were in clusters in tear-deficient dogs. A hypothesis that petrolatum/mineral oil ointment should provide more effective artificial tear replacement than hydroxymethylcellulose drops for tear-deficient dogs could not be confirmed by objective analysis of corneal dark-cell density or conjunctival goblet cell density.

Goblet cell density association with tear function and ocular surface physiology

2015 ◽  
Vol 38 (4) ◽  
pp. 240-244 ◽  
Author(s):  
Kishor Sapkota ◽  
Sandra Franco ◽  
Paula Sampaio ◽  
Madalena Lira
Keyword(s):  
Cell Density ◽  
Goblet Cell ◽  
Ocular Surface ◽  
Goblet Cell Density

scholarly journals Distribution and density of conjunctival goblet cells

2002 ◽  
Vol 55 (5-6) ◽  
pp. 195-200 ◽  
Author(s):  
Vesna Vujkovic ◽  
Gostimir Mikac ◽  
Risto Kozomara
Keyword(s):  
Cell Density ◽  
Dry Eye ◽  
Goblet Cell ◽  
Tear Film ◽  
Goblet Cells ◽  
Dry Eye Syndrome ◽  
Bulbar Conjunctiva ◽  
Cell Distribution ◽  
Significant Difference ◽  
Goblet Cell Density

There is a regional variation in conjunctival goblet cell distribution and density per unit of measurement. By secreting mucin goblet cells are involved in formation of tear film. Tear film instability is the basic sign of dry eye syndrome. Although dry eye syndrome can be a side effect of a disease, the most common cause is normal aging. The objective of the research was to determine goblet cell density in bulbar and lower forniceal conjunctiva and possible changes in goblet cell density related to age and sex. The research was conducted on 30 conjunctival samples of patients with no clinical changes of the conjunctiva. Tissue sections were stained with hematoxylin eosin (HE), periodic acid-schiff (PAS), alcian blue (AB)/PAS Giemsa methods. Goblet cell density was 1.24?1.62 in bulbar conjunctiva and 30.21?14.32 in lower forniceal conjunctiva. Goblet cell distribution in the conjunctiva was unequal. A correlation between goblet cell density and age was not determined. Goblet cell density doesn't decrease with aging. No significant difference in goblet cell density between men and women was established.

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