scholarly journals VOLUME DE MEIO E NÚMERO DE OÓCITOS PARA FECUNDACÃO NA PRODUÇÃO IN VITRO DE EMBRIÕES BOVINOS

2004 ◽  
Vol 9 (2) ◽  
Author(s):  
D.S. BRUM ◽  
F.G. LEIVAS ◽  
M.R. NOEBAUER ◽  
M.L. BERNARDI ◽  
C.A.M. SILVA ◽  
...  
Keyword(s):  
In Vitro Maturation ◽  
Hatching Rate ◽  
Bovine Embryos ◽  
In Vitro Production ◽  
Oviduct Fluid ◽  
Vitro Production ◽  
Hatching Rates

A origem dos Complexos Cumulus-Oócitos (CCO) bovinos e os diferentes protocolos entre equipes geram variações nos índices de produção in vitro (PIV). O processo pode ser influenciado pelo número de CCO e volume das gotas de maturação, fecundação e cultivo. Neste estudo, avaliou-se a densidade de CCO e o volume de meio de fecundação sobre a PIV com 672 oócitos bovinos. A maturação in vitro (MIV) foi conduzida com 20 CCO em 200µL de TCM-199+rFSHh+10% de soro de vaca em estro (SVE), por 24h, em estufa a 39ºC, 5% CO2 em ar e umidade saturada. Na fecundação (Dia 0 =D0) utilizou-se 5, 10 ou 20 CCO e 2 volumes de meio (1:5 e 1:10), num fatorial 3x2, em 6 repetições. O sêmen foi selecionado por migração ascendente e para a fertilização (1x106 espermatozóides/mL) os gametas foram incubados em Fert-Talp, por 18h. O cultivo foi em grupos de 20 zigotos, em 200µL de fluído sintético do oviduto (SOF) aaci+5%SVE, por 8 dias e as avaliações em D2 (clivagem), D7 (Blastocistos), D9 (Blastocistos expandidos, em eclosão e eclodidos). As taxas de clivagem (80, 85 e 87%), embriões em D9 (23, 27 e 28%) e de eclosão (42, 37 e 48%) foram semelhantes (P>0,05) para 5, 10 ou 20 CCO, respectivamente. Não houve diferença (P>0,05) na clivagem (83 e 85%), embriões em D9 (24 e 28%) e eclosão (41 e 44%) entre as proporções 1:5 e 1:10µL. A produção de blastocistos e taxa de eclosão em D9 não são influenciados pelo número de CCO, nem pela proporção de CCO por volume de meio na fecundação. Volume of medium and number of oocytes for fertilization in the in vitro production of bovine embryos Abstract The origin of the bovine Cumulus Oocytes Complexes (COC) and the different protocols among teems are responsible for the variation rates of the in vitro production (IVP). The process is also influenced by COC number and by the volume of the drop in the maturation, fertilization and culture. In this study, the effect of bovine COC density per drop medium and the fertilization medium volume were evaluated during the bovine IVP using 672 COC. The in vitro maturation was performed with 20 COC in 200µL of TCM-199+rFSHh+10% estrus cow serum (ECS), for 24h in an incubator with 5%CO2 in air, saturated humidity, at 39oC. For the fertilization (Day 0 = D0) 5, 10 or 20 COC and 2 volumes ratio of medium (1:5 and 1:10) were used in a factorial outline 3x2, in 6 replications. Thawed semen was prepared by a swim up process and for the fertilization (1x106 spermatozoa/ ml) the gametes were maintained in Fert-Talp for 18h. Groups of twenty presumed zygotes were cultured in 200µL synthetic oviduct fluid (SOFaaci)+5% ECS, for 8 days. The evaluations were carried out in D2 (cleavage), D7 (blastocysts) and D9 (expanded and hatched blastocyts). The rates of cleavage (80, 85 and 87%), embryos in D9/ D2 (23, 27 and 28%) and hatched blastocstys in D9/D7 (42, 37 and 48%) were similar (P>0.05) for 5, 10 and 20 COC, respectively. No difference was observed in cleavage (83 and 85%), embryos in D9 (24 and 28%) and hatching rates (41 and 44%) for 1:5 and 1:10µL of fertilization medium. Blastocysts production and the hatching rate in D9 were neither influenced by the COC number, nor by the proportion of COC per medium volume used during fertilization.

scholarly journals Use of Melatonin in the In Vitro Production of Bovine Embryos

2020 ◽  
Vol 21 ◽  
Author(s):  
Alan da Silva LIRA ◽  
Ricardo de Macedo CHAVES ◽  
Felipe de Jesus MORAES JUNIOR ◽  
Sergio Henrique COSTA JUNIOR ◽  
Brenda Karine Lima do AMARAL ◽  
...  
Keyword(s):  
Oocyte Maturation ◽  
In Vitro Maturation ◽  
Control Group ◽  
Bovine Embryos ◽  
In Vitro Production ◽  
Maturation Medium ◽  
Significant Difference ◽  
Maturation Rate ◽  
Vitro Production

ABSTRACT We aimed to assess the effects of melatonin in the in vitro production of bovine embryos. Our experiment was conducted at the Laboratório de Reprodução Animal of the Universidade Estadual do Maranhão. The cumulus-oocyte complexes (COCs) were distributed among treatments at concentrations of 0, 10-1, 10-3 and 10-5 µMol/L melatonin. Our experiment was further divided into two: the first was to assess the effect of different concentrations of melatonin (treatments) on the maturation rate of COCs, and the second was to assess the effects of melatonin treatments on the in vitro production of bovine embryos. The results from the first experiment demonstrated no significant difference between the in vitro maturation rate of the cultivated COCs in treatments with melatonin. In the second experiment, however, melatonin treatments yielded statistically higher cleavage, morula and blastocyst rates in the 10-5 µM group (52.9%, 52.9%, and 35.3%, respectively), and lower rates in the 10-1 µM group (19.5%, 19.5% and 7.8%, respectively), compared to the others. The control group (no melatonin) and the 10-3 µM group showed similar results. We concluded that supplementation of melatonin in the in vitro maturation medium resulted in no improvement in the oocyte maturation rate, but in the in vitro production of embryos at different concentrations, the 10-5 µM group displayed better results, but with no improvement in the variables (P < 0.05).

scholarly journals Supplementation of in vitro maturation medium with serum from dairy cows at early and late lactation in the in vitro production of bovine embryos

2018 ◽  
Vol 12 (4) ◽  
pp. 311
Author(s):  
Letícia Franco Collares ◽  
Jorgea Pradieé ◽  
Morgana Alves Borges ◽  
Bruna Mion ◽  
Patrícia Gindri ◽  
...  
Keyword(s):  
Dairy Cows ◽  
In Vitro Maturation ◽  
Bovine Embryos ◽  
In Vitro Production ◽  
Maturation Medium ◽  
Vitro Production

Noninvasive characterization of metabolites secreted in culture media by bovine embryos during in vitro production

Metabolomics ◽  
2016 ◽  
Vol 12 (5) ◽  
Author(s):  
Érika Cristina dos Santos ◽  
Camila Bruna de Lima ◽  
Kelly Annes ◽  
Marcella Pecora Milazzotto
Keyword(s):  
Culture Media ◽  
Bovine Embryos ◽  
In Vitro Production ◽  
Noninvasive Characterization ◽  
Vitro Production

Animal factors that influence the in-vitro production of bovine embryos

1999 ◽  
Vol 51 (1) ◽  
pp. 334 ◽  
Author(s):  
H Twagiramungu ◽  
N Morin ◽  
C Brisson ◽  
G Carbonneau ◽  
J Durocher ◽  
...  
Keyword(s):  
Bovine Embryos ◽  
In Vitro Production ◽  
Vitro Production

scholarly journals Resveratrol-supplemented holding or re-culture media improves viability of fresh or vitrified-warmed in vitro-derived bovine embryos

2021 ◽  
Vol 10 (14) ◽  
pp. e367101422097
Author(s):  
Arianny Rafaela Neto Silva ◽  
Thaisa Campos Marques ◽  
Elisa Caroline Silva Santos ◽  
Tiago Omar Diesel ◽  
Isabelle Matos Macedo ◽  
...  
Keyword(s):  
Culture Media ◽  
Cell Number ◽  
Time Dependent ◽  
Expansion Rate ◽  
Ros Generation ◽  
Hatching Rate ◽  
Protective Effects ◽  
Bovine Embryos ◽  
In Vitro Production

The effect of resveratrol supplementation on fresh (E1) or vitrified/warmed (E2) in vitro produced bovine embryos was investigated by evaluating the time-dependent response. After in vitro production, resveratrol (0.5 µM) was added to the incubation media and after two incubation periods with or without resveratrol, blastocysts were re-cultured for 24h. The rates of re-expansion, hatching, total cell number (TCN), apoptotic cells (ACN), reactive oxygen species (ROS) and intracellular glutathione (GSH) content were evaluated. For E1, the re-expansion rate differed at 6 and 10h within and between treatments (P<0.05), as did the re-expansion rate after 24h (P<0.01). The hatching rate increased after 10h with resveratrol (P<0.01) with differences within (P<0.05), but not between treatments after 24h of re-cultivation. At E2, hatching rate differed between treatments at 24h (P<0.01), with higher TCN in resveratrol-treated blastocysts after 10h (P<0.01). Resveratrol supplementation reduced ROS generation in E1 and E2 after 10h of incubation and increased GSH content (P<0.01). These results indicate that supplementation of holding re-cultivation medium with resveratrol for treatment of fresh or vitrified/warmed in vitro produced bovine embryos has a positive and time-dependent effect. The reduction of ROS content, the increase of GSH and the anti-apoptotic ability of resveratrol are responsible for its protective effects, allowing an extension of embryo storage time before transfer to recipients.

65 Comparison of SexedULTRA-4M™ X and Y chromosome-bearing semen versus conventional semen for the in vitro production of bovine embryos

2022 ◽  
Vol 34 (2) ◽  
pp. 268
Author(s):  
A. Velázquez-Roque ◽  
H. Álvarez-Gallardo ◽  
M. Kjelland ◽  
M. Pérez-Martínez ◽  
F. Villaseñor-González ◽  
...  
Keyword(s):  
Y Chromosome ◽  
Bovine Embryos ◽  
In Vitro Production ◽  
Vitro Production

Evaluation of different culture systems on the in vitro production of bovine embryos

2005 ◽  
Vol 63 (4) ◽  
pp. 1131-1141 ◽  
Author(s):  
Daniela Costa Pereira ◽  
Margot Alves Nunes Dode ◽  
Rodolfo Rumpf
Keyword(s):  
Bovine Embryos ◽  
In Vitro Production ◽  
Culture Systems ◽  
Vitro Production
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