UV SPECTROPHOTOMETRIC ABSORPTION CORRECTION METHOD FOR SIMULTANEOUS ESTIMATION OF OLMESA RTAN MEDOXOMIL, AMLODIPINE BESYLATE AND HYDROCHLOROTHIAZIDE IN COMBINED TABLET DOSAGE FORM

A simple, accurate, precise, economical and validated uv spectrometric absorption correction method was developed for olmesartan, medoxomil amlodipine besylate and hydrochlorothiazide in bulk and its dosage form. The stock solutions were prepared in methanol followed by further dilutions with double distilled water. The λmax for olmesartan, amlodipine besylate and hydrochlorothiazide were 256.5 nm, 362 nm and 316 nm, respectively. Beer’s law was obeyed in the concentration range of 3-18 μg/mL, 1- 6μg/mL and 2-12 μg/mL, respectively. The method was validated by following the analytical performance parameters suggested by the ICH Guidelines. The percentage assay in commercial formulation was found to be in the range olmesartan 100.06%, amlodipine 99.17% and hydrochlorothiazide 100.14% by the proposed method. The method was validated with respect to linearity, precision and accuracy. recovery was found to be in the range of olmesartan 99.65%,amlodipine 99.97% and hydrochlorothiazide 99.81% by absorbance corrected method. The high recovery and low coefficients of variation conforms the suitability of the method for simultaneous analysis of three drugs in combined tablets.

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Absorbance Correction Method for Simultaneous Estimation of Nifedipine and Metoprolol Succinate in Their Synthetic Mixture Using From Spectrophotometry

International Journal of Advances in Scientific Research ◽

10.7439/ijasr.v1i3.1774 ◽

2015 ◽

Vol 1 (3) ◽

pp. 151

Author(s):

Sojitra Rajanit ◽

Paras Virani ◽

Hashumati Raj

Keyword(s):

Accurate Method ◽

Correction Method ◽

Synthetic Mixture ◽

Simultaneous Estimation ◽

Metoprolol Succinate ◽

Absorption Correction ◽

Ich Guidelines ◽

Development And Validation ◽

Tablet Dosage

A new simple, economical, precise and accurate method are described for the simultaneous determination of Nifedipine (NIF) and Metoprolol Succinate (MET) in combined tablet dosage form. The proposed method was applied for the determination of Nifedipine and Metoprolol Succinate in synthetic mixture, for determination of sampling wavelength, 10?g/ml of each of NIF and MET were scanned in 200-400 nm range and sampling wavelengths were 313nm for NIF and 275.40nm for MET are selected for development and validation of absorption correction method. For this method linearity observed in the range of 5-25?g/ml for NIF and 25-125?g/ml for MET, and in their pharmaceutical formulation with mean percentage recoveries 100.68 and 100.33, respectively. The method was validated according to ICH guidelines and can be applied for routine quality control testing.

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VALIDATED ABSORPTION CORRECTION METHOD FOR SIMULTANEOUS ESTIMATION OF ROSUVASTATIN CALCIUM AND EZETIMIBE IN TABLET DOSAGE FORM

World Journal of Pharmaceutical Research ◽

10.20959/wjpr20176-8433 ◽

2017 ◽

pp. 679-690

Author(s):

V. Raut

Keyword(s):

Dosage Form ◽

Correction Method ◽

Simultaneous Estimation ◽

Absorption Correction ◽

Tablet Dosage ◽

Rosuvastatin Calcium

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Q-Absorbance Ratio Spectrophotometric Method for the Simultaneous Estimation of Metformin Hydrochloride and Voglibose in Tablet Dosage form

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00554 ◽

2021 ◽

pp. 3179-3183

Author(s):

Kedar Tejashree R. ◽

A.R. Dashetwar ◽

D.P. Kardile ◽

A.P. Jadhav ◽

V.C. Bhagat ◽

...

Keyword(s):

Spectrophotometric Method ◽

Dosage Form ◽

Ratio Method ◽

Metformin Hydrochloride ◽

Simultaneous Estimation ◽

Absorbance Ratio ◽

Ich Guidelines ◽

Highly Sensitive ◽

Stock Solutions ◽

Tablet Dosage

A new, simple, accurate, precise and reproducible UV-Spectrophotometric method is being developed for the simultaneous estimation of Metformin Hydrochloride and Voglibose in tablet dosage form. The stock solutions were prepared in methanol. The λmax for Metformin Hydrochloride and Voglibose were found to be248 nm and 287nm respectively. The Metformin Hydrochloride and Voglibose obeyed Beer’s law in concentration range of 8-16µg/ml and 4-20µg/ml respectively. Results of analysis of absorbance ratio method were analysed and validated for various parameters according to ICH guidelines for accuracy, precision, linearity, robustness, LOD and LOQ. The proposed method is highly sensitive, precise and accurate, therefore can be used for intended purpose.

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Simultaneous Spectrophotometric Estimation of Telmisartan and Amlodipine Besylate in Tablet Dosage Form

Indian Journal of Pharmaceutical and Biological Research ◽

10.30750/ijpbr.3.3.8 ◽

2015 ◽

Vol 3 (03) ◽

pp. 50-54 ◽

Cited By ~ 1

Author(s):

N. K. Gupta ◽

A. Peepliwal ◽

D. S. Rathore ◽

P. Gupta

Keyword(s):

Quality Control ◽

Dosage Form ◽

Simultaneous Equation ◽

Dosage Forms ◽

Simultaneous Estimation ◽

Amlodipine Besylate ◽

Spectrophotometric Methods ◽

Ich Guidelines ◽

Tablet Dosage ◽

Routine Quality Control

A simple, accurate and reproducible spectrophotometric methods have been developed for the simultaneous estimation of Telmisartan (TEL) and Amlodipine Besylate (AML) in combined tablet dosage forms. The method involves determination using the simultaneous equation method, the sampling wavelengths selected are ‘TLM’ = 297nm.and ‘AML’ =238nm., over the concentration ranges of 8-48μg/ml for ‘TEL’ and 1-6 μg/ml for ‘AML’ respectively. The method was validated for linearity, accuracy, precision, robustness and application for assay as per ICH guidelines. The proposed method is simple, economical, accurate and precise, and could be successfully employed in routine quality control for the simultaneous analysis of Telmisartan (TEL) and Amlodipine Besylate (AML).

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Development and Validation of Absorption Correction Method for Simultaneous Estimation of Paracetamol and Nimesulide in Bulk and Combined Tablet Dosage Form

Asian Journal of Pharmaceutical Analysis ◽

10.5958/2231-5675.2018.00006.6 ◽

2018 ◽

Vol 8 (1) ◽

pp. 33

Author(s):

Dnyaneshwari D. Waichal ◽

Vrushali S. Tambe

Keyword(s):

Dosage Form ◽

Correction Method ◽

Simultaneous Estimation ◽

Absorption Correction ◽

Development And Validation ◽

Tablet Dosage

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STABILITY-INDICATING RP-HPLC METHOD FOR THE SIMULTANEOUS DETERMINATION OF AMLODIPINE BESYLATE AND AZILSARTAN MEDOXOMIL IN TABLET DOSAGE FORM

INDIAN DRUGS ◽

10.53879/id.53.06.10500 ◽

2016 ◽

Vol 53 (06) ◽

pp. 51-61

Author(s):

J. G Modi ◽

◽

J. K. Patel

Keyword(s):

Mobile Phase ◽

Dosage Form ◽

Hplc Method ◽

Simultaneous Estimation ◽

Good Resolution ◽

Amlodipine Besylate ◽

Stability Indicating ◽

Rp Hplc ◽

Tablet Dosage ◽

Azilsartan Medoxomil

A novel, simple, rapid, and highly selective stability indicating RP-HPLC method was developed and validated for simultaneous estimation of azilsartan medoxomil (AZL) and amlodipine besylate HCl (AMLO) in tablet dosage form having strength of 20 mg and 2.5 mg, respectively. The effective chromatographic separation was achieved on a Phenomenex luna ODS C18 (15 cm X 4.6 mm internal diameter, 3.5 μm Particle size) with a mobile phase composed of phosphate buffer (pH-2.5) adjusted with ortho phosphoric acid : acetonitrile in the ratio of 60:40 v/v. The mobile phase was pumped using an isocratic HPLC system at a flow rate of 0.7 mL/min with injection volume 20μl and quantification of the analytes was done at detection wavelength 254 nm. The retention times were found to be 5.918 min and 14.901 min for AMLO and AZL, respectively. The proposed HPLC method was validated with respect to linearity, ranges, precision, accuracy, specificity, robustness, LOD, and LOQ as per ICH Q2 (R1) guideline. Calibration plots were linear over the concentration range of 75-125 µg/mL and 600-1000 µg/mL with correlation coefficients 0.9966 and 0.9948 for AMLO and AZL, respectively. Forced degradation studies were performed using hydrolysis, oxidation, photolytic, and thermal degradation conditions with good resolution between the degradants and analytes. Degradation products resulting from the stress studies did not interfere with the detection of AMLO and AZL, thus the proposed method is sensitive and stability-indicating. The validated HPLC method was successfully applied to the analysis of AMLO and AZL in tablet dosage form.

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SIMULTANEOUS ESTIMATION OF AMLODIPINE BESYLATE AND INDAPAMIDE BY DUAL WAVELENGTH SPECTROPHOTOMETRIC METHOD FOR COMBINED PHARMACEUTICAL DOSAGE FORM

INDIAN DRUGS ◽

10.53879/id.51.04.p0050 ◽

2014 ◽

Vol 51 (04) ◽

pp. 50-54

Author(s):

M. P Patel ◽

◽

M. R Patel ◽

R Hasumati ◽

M. N Noolvi ◽

...

Keyword(s):

Spectrophotometric Method ◽

Dosage Form ◽

Dual Wavelength ◽

Simultaneous Estimation ◽

Uv Spectrophotometry ◽

Amlodipine Besylate ◽

Pharmaceutical Dosage Form ◽

First Order ◽

Absorbance Difference ◽

Tablet Dosage

A simple, accurate, precise, rapid, economical UV spectrophotometry method, dual wavelength spectrophotometry, has been developed and validated for estimation of Indapamide (IND) and Amlodipine besylate (AML) in combined tablet dosage form and can be used in routine analysis. In this method, the absorbance at 360 nm and 256 nm of AML were same and no interference of IND at 360 nm. was observed. So, absorbance difference at 256-360 is used for estimation of IND and absorbance at 360 nm used for AML. The method was found to be linear in the concentration range of 3-18 μg/mL for IND (r2>0.99962) and 10-60 μg/mL for AML (r2>0.99969). Mean assay was found to be 99.32% and 101.34% for IND and AML respectively. In first order derivative spectrophotometry, the absorbance at 237.4 nm (ZCP of AML) and 241 nm (ZCP of IND) were used for estimation of IND and AML respectively. The method was found to be linear in the concentration range of 1.5-9 μg/mL for IND(r2=0.99983) and 5-30 μg/mL for AML(r2=0.99966). Mean assay was found to be 99.72% and 100.28% for IND and AML respectively.

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A NEW VALIDATED THIRD ORDER DERIVATIVE SPECTROSCOPIC METHOD FOR SIMULTANEOUS ESTIMATION OF METOPROLOL SUCCINATE AND RAMIPRIL IN TABLET DOSAGE FORM

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2020v12i5.36413 ◽

2020 ◽

pp. 54-59

Author(s):

ALEKHYA B. ◽

M. SINDHUSHA ◽

SORAJ K. RAUL ◽

GOPAL K. PADHY

Keyword(s):

Quantitative Analysis ◽

Dosage Form ◽

Spectroscopic Method ◽

Simultaneous Estimation ◽

Third Order ◽

Metoprolol Succinate ◽

Zero Crossing ◽

Ich Guidelines ◽

Tablet Dosage

Objective: The objective of the present work is to develop and validate a new UV derivative spectrophotometric method for simultaneous estimation of metoprolol succinate and ramipril in methanol: water (50:50v/v). Methods: “Zero crossing technique” was chosen for quantitative determination. The zero-crossing points (ZCP’s) were found to be 209 nm where metoprolol succinate was quantified and 211 nm where ramipril was quantified. This method was then subjected to accuracy, linearity, sensitivity and reproducibility according to ICH guidelines to ensure and confirm its validity. Results: The method was found to be obeying Beer’s law in the range of 10-50 µg/ml and 5-25 µg/ml for metoprolol succinate and ramipril, respectively. The % recoveries were observed between the range of 99.2-100.2 for metoprolol succinate and 99.57-99.86 for ramipril. The intra-day and inter-day results showed reproducibility. Conclusion: It can be concluded that the developed third-order UV derivative spectroscopic method for the simultaneous determination of metoprolol succinate and ramiprilcan be recommended for routine quantitative analysis.

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