scholarly journals Absorbance Correction Method for Simultaneous Estimation of Nifedipine and Metoprolol Succinate in Their Synthetic Mixture Using From Spectrophotometry

2015 ◽  
Vol 1 (3) ◽  
pp. 151
Author(s):  
Sojitra Rajanit ◽  
Paras Virani ◽  
Hashumati Raj
Keyword(s):  
Accurate Method ◽  
Correction Method ◽  
Synthetic Mixture ◽  
Simultaneous Estimation ◽  
Metoprolol Succinate ◽  
Absorption Correction ◽  
Ich Guidelines ◽  
Development And Validation ◽  
Tablet Dosage

A new simple, economical, precise and accurate method are described for the simultaneous determination of Nifedipine (NIF) and Metoprolol Succinate (MET) in combined tablet dosage form. The proposed method was applied for the determination of Nifedipine and Metoprolol Succinate in synthetic mixture, for determination of sampling wavelength, 10?g/ml of each of NIF and MET were scanned in 200-400 nm range and sampling wavelengths were 313nm for NIF and 275.40nm for MET are selected for development and validation of absorption correction method. For this method linearity observed in the range of 5-25?g/ml for NIF and 25-125?g/ml for MET, and in their pharmaceutical formulation with mean percentage recoveries 100.68 and 100.33, respectively. The method was validated according to ICH guidelines and can be applied for routine quality control testing.

scholarly journals A NEW VALIDATED THIRD ORDER DERIVATIVE SPECTROSCOPIC METHOD FOR SIMULTANEOUS ESTIMATION OF METOPROLOL SUCCINATE AND RAMIPRIL IN TABLET DOSAGE FORM

2020 ◽  
pp. 54-59
Author(s):  
ALEKHYA B. ◽  
M. SINDHUSHA ◽  
SORAJ K. RAUL ◽  
GOPAL K. PADHY
Keyword(s):  
Quantitative Analysis ◽  
Dosage Form ◽  
Spectroscopic Method ◽  
Simultaneous Estimation ◽  
Third Order ◽  
Metoprolol Succinate ◽  
Zero Crossing ◽  
Ich Guidelines ◽  
Tablet Dosage

Objective: The objective of the present work is to develop and validate a new UV derivative spectrophotometric method for simultaneous estimation of metoprolol succinate and ramipril in methanol: water (50:50v/v). Methods: “Zero crossing technique” was chosen for quantitative determination. The zero-crossing points (ZCP’s) were found to be 209 nm where metoprolol succinate was quantified and 211 nm where ramipril was quantified. This method was then subjected to accuracy, linearity, sensitivity and reproducibility according to ICH guidelines to ensure and confirm its validity. Results: The method was found to be obeying Beer’s law in the range of 10-50 µg/ml and 5-25 µg/ml for metoprolol succinate and ramipril, respectively. The % recoveries were observed between the range of 99.2-100.2 for metoprolol succinate and 99.57-99.86 for ramipril. The intra-day and inter-day results showed reproducibility. Conclusion: It can be concluded that the developed third-order UV derivative spectroscopic method for the simultaneous determination of metoprolol succinate and ramiprilcan be recommended for routine quantitative analysis.

Analytical Method Development and Validation for Telmisartan, Chlorthalidone and Amlodipine by UV- Spectroscopic Method.

2021 ◽  
pp. 6049-6054
Author(s):  
Rutuja M. Sanap ◽  
Sarika R. Wavhale ◽  
Vaibhavi V. Kunjir ◽  
Rajkumar V. Shete
Keyword(s):  
Simultaneous Determination ◽  
Analytical Method ◽  
Dosage Form ◽  
Method Development ◽  
Correction Method ◽  
Absorption Correction ◽  
Method Development And Validation ◽  
Development And Validation ◽  
Tablet Dosage

A simple, sensitive and accurate UV- spectrophotometric absorption correction method has been developed for simultaneous determination of Telmisartan, Amlodipine and Chlorthalidone in combined tablet dosage form. Analytical method development and validation plays important role in the discovery manufacture of pharmaceuticals and development. In this paper, absorption correction method is used for multi-component analysis. The wavelengths selected for the analysis were 311nm for Telmisartan, 228nm for Chlorthalidone and 253nm for Amlodipine. Beer’s law obeyed the concentration range of 2-10 µg /ml, 2-10 µg /ml and 5-25 µg/ ml for Telmisartan, Amlodipine and Chlorthalidone. Methanol is used as a solvent. The accuracy of the method was assessed by recovery studies and was found between the range of 100% to 110% for Telmisartan, 85% to 110% for Amlodipine and 85% to 105% Chlorthalidone. The % RSD value was found to be less than 2. Thus, the method was simple, precise, economic, rapid, accurate and can be successfully applied for simultaneous determination of Telmisartan, Amlodipine and Chlorthalidone in combined tablet dosage form.

scholarly journals Stability indicating RP-HPLC method development and validation for the simultaneous estimation of ceftriaxone and tazobactum in sterile powder for injection

2019 ◽  
Vol 1 (2) ◽  
pp. 40-43
Author(s):  
T. Vimalakkannan ◽  
P. Shaik Parveen ◽  
Salomi ◽  
K. Ravindra Reddy
Keyword(s):  
Method Development ◽  
Pharmaceutical Formulations ◽  
Accurate Method ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
Ich Guidelines ◽  
Method Development And Validation ◽  
Hplc Method Development ◽  
Development And Validation

A simple, rapid, precise and accurate method is developed for the quantitative simultaneous determination of ceftriaxone and tazobactum in bulk and pharmaceutical formulations. Separation of ceftriaxone and tazobactum was successfully achieved by using Inertsil C18 ODS column 250X4.6mm, 5µm in an isocratic mode using water and acetonitrile (80:20) at a flow rate of 1.0 ml/min and was monitored at 254 nm with a retention time of 3.049 minutes and 4.317 minutes for ceftriaxone and tazobactum respectively. The method was validated and the response was found to be linear in the drug concentration range of 20µg/ml to 80 µg/ml for ceftriaxone and 5 µg/ml to 35 µg/ml for tazobactum. The values of the correlation coefficient were found to be 0.999 for ceftriaxone and 0.999 for tazobactum respectively. The LOD and LOQ for ceftriaxone were found to be 0.021 and 0.064 respectively. The LOD and LOQ for tazobactum were found to be 0.030 and 0.091 respectively. The percentage recovery for ceftriaxone and tazobactum were found to be 98-102% respectively which indicates that the proposed method is highly accurate. The specificity of the method shows good correlation between retention times of standard with the sample. The method was extensively validated according to ICH guidelines for Linearity, Accuracy, Precision, Specificity and Robustness.  Stability of the drugs was determined by using acid/base, thermal, oxidative stress testing.

DEVELOPMENT AND VALIDATION OF A NEW ABSORBANCE CORRECTION METHOD FOR ESTIMATION OF TELMISARTAN AND METOPROLOL SUCCINATE IN COMBINED TABLET DOSAGE FORM

INDIAN DRUGS ◽  
2014 ◽  
Vol 51 (08) ◽  
pp. 40-43
Author(s):  
Nimisha S Priolkar ◽  
◽  
L. Almeida
Keyword(s):  
Hydrochloric Acid ◽  
Dosage Form ◽  
Correction Method ◽  
Metoprolol Succinate ◽  
International Conference ◽  
Ich Guidelines ◽  
Development And Validation ◽  
Tablet Dosage ◽  
Correction Equations

A simple and accurate absorbance correction method was developed for the estimation of telmisartan and metoprolol succinate in combined tablet dosage form. The method is based on use of absorbance correction equations for analysis of both the drugs using ethanol and hydrochloric acid (0.1N) in the ratio 1:1 as a solvent. The wavelengths used for analysis were 334 nm and 275 nm respectively, for telmisartan and metoprolol succinate. Linearity was found in the concentration range of 10 -100 µg/ mL, for both the drugs. The method was validated in accordance with the International Conference on Harmonisation (ICH) guidelines. The proposed method was found to be simple, accurate and sensitive for estimation of telmisartan and metoprolol succinate in combined tablet dosage form.

UV SPECTROPHOTOMETRIC ABSORPTION CORRECTION METHOD FOR SIMULTANEOUS ESTIMATION OF OLMESA RTAN MEDOXOMIL, AMLODIPINE BESYLATE AND HYDROCHLOROTHIAZIDE IN COMBINED TABLET DOSAGE FORM

INDIAN DRUGS ◽  
2017 ◽  
Vol 54 (07) ◽  
pp. 40-45
Author(s):  
J. Saminathan ◽  
◽  
T. Vetrichelvan
Keyword(s):  
Dosage Form ◽  
Olmesartan Medoxomil ◽  
Correction Method ◽  
Simultaneous Estimation ◽  
Amlodipine Besylate ◽  
Absorption Correction ◽  
Ich Guidelines ◽  
Coefficients Of Variation ◽  
Stock Solutions ◽  
Tablet Dosage

A simple, accurate, precise, economical and validated uv spectrometric absorption correction method was developed for olmesartan, medoxomil amlodipine besylate and hydrochlorothiazide in bulk and its dosage form. The stock solutions were prepared in methanol followed by further dilutions with double distilled water. The λmax for olmesartan, amlodipine besylate and hydrochlorothiazide were 256.5 nm, 362 nm and 316 nm, respectively. Beer’s law was obeyed in the concentration range of 3-18 μg/mL, 1- 6μg/mL and 2-12 μg/mL, respectively. The method was validated by following the analytical performance parameters suggested by the ICH Guidelines. The percentage assay in commercial formulation was found to be in the range olmesartan 100.06%, amlodipine 99.17% and hydrochlorothiazide 100.14% by the proposed method. The method was validated with respect to linearity, precision and accuracy. recovery was found to be in the range of olmesartan 99.65%,amlodipine 99.97% and hydrochlorothiazide 99.81% by absorbance corrected method. The high recovery and low coefficients of variation conforms the suitability of the method for simultaneous analysis of three drugs in combined tablets.

scholarly journals Development and Validation of Absorption Correction UV-Spectrophotometry Method for Simultaneous Estimation of Ranitidine HCL and Dicyclomine Hydrochloride in Their Marketed Formulation

2019 ◽  
pp. 851-863
Author(s):  
Bhumi Kantariya ◽  
Greeva Bhatt ◽  
Mehul Mehta ◽  
Urmi Kanatria ◽  
Ravi Dalsaniya
Keyword(s):  
Dosage Form ◽  
Simultaneous Estimation ◽  
Uv Spectrophotometry ◽  
Rapid Absorption ◽  
Absorption Correction ◽  
Naoh Solution ◽  
Development And Validation ◽  
Tablet Dosage ◽  
Ich Guideline

A Simple, selective, precise and rapid absorption correction Spectrophotometric method has been developed and validated as per ICH guideline for the simultaneous estimation of Ranitidine Hydrochloride and Dicyclomine Hydrochloride in tablet dosage form. Method is base on UV Spectrophotometric for determination of two drug, by using Methanol as a solvent and diluted the same with 0.1N NaOH, solution. In this UV method, the two wavelength were selected, 311.4 nm and at 217 nm for RANTD and DICY, respectively. this method was validated according to ICH guideline and Linearity range, was found to be 7.5-37.5 µg/ml and 1-5 µg/ml for RANTD and DICY, respectively. The method was successfully applied to assay drugs in tablet.

Development and Validation of Derivative Spectroscopic Method for Simultaneous Estimation of Cefadroxil and Probenecid

2014 ◽  
Vol 7 (1) ◽  
pp. 2350-2355
Author(s):  
Pratik S Mehta ◽  
Pratik R. Patel ◽  
Rajesh R Parmar ◽  
M M K Modasiya ◽  
Dushyant A Shah
Keyword(s):  
Spectroscopic Method ◽  
Spectral Interference ◽  
Synthetic Mixture ◽  
Simultaneous Estimation ◽  
Zero Crossing ◽  
First Order ◽  
Derivative Spectroscopy ◽  
Development And Validation ◽  
Crossing Point

A novel, simple, accurate, sensitive, precise and economical derivative spectroscopic method was developed and validated for the determination of cefadroxil and probenecid in synthetic mixture. First order derivative spectroscopy method was adopted to eliminate spectral interference. The method obeys Beer’s Law in concentration ranges of 4-36 μg/ml for cefadroxil and of 5-25 μg/ml of probenecid. The zero crossing point for cefadroxil and probenecid was 260 nm and 237.8 nm respectively in 0.1N HCl. The method was validated in terms of accuracy, precision, linearity, limits of detection, limits of quantitation. This method has been successively applied to synthetic mixture and no interference from the synthetic mixture’s excipients was found.   

scholarly journals LC-MS/MS METHOD DEVELOPMENT AND VALIDATION FOR THE DETERMINATION OF CARDIAZOL IN HUMAN PLASMA

2019 ◽  
pp. 380-385
Author(s):  
IRYNA DRAPAK ◽  
BORYS ZIMENKOVSKY ◽  
LINA PEREKHODA ◽  
SERGIY KOVALENKO ◽  
Liliya Logoyda
Keyword(s):  
Formic Acid ◽  
Human Plasma ◽  
Method Development ◽  
Accurate Method ◽  
Sample Volume ◽  
Ich Guidelines ◽  
Coefficients Of Variation ◽  
Method Development And Validation ◽  
Development And Validation

Objective: The main purpose of this study was to develop a simple, precise, rapid and accurate method for the quantification of cardiazol in human plasma. Methods: Chromatography was achieved on Discovery C18, 50 × 2.1 mm, 5 μm column. Samples were chromatographed in a gradient mode (eluent A (acetonitrile-water–formic acid, 5: 95: 0.1 v/v), eluent B (acetonitrile–formic acid, 100: 0.1 v/v)). The initial content of the eluent B of 8%, which increases linearly to 1.0 min to 100%, is maintained up to 1.5 min and returned to the original 8% to 1.51 min. The mobile phase was delivered at a flow rate of 0.400 ml/min into the mass spectrometer ESI chamber. The sample volume was 300 μl. Results: The total chromatographic run time was 2.5 min and the elution of cardiazol and IS (difenoconazole) occurred at ~2.15 and 1.98 min, respectively. A linear response function was established at 1-100 ng/ml for cardiazol and difenoconazole in human plasma. The % mean recovery for cardiazol in LQC, MQC and HQC was 102.8 %, 100.3 % and 95.9 %. The lowest concentration with the RSD<20% was taken as LLOQ and was found to be 1.10 ng/ml for cardiazol. The % accuracy of LLOQ samples prepared with the different biological matrix lots was found 109.7 %, which were found within the range of 80.00-120.00 % for the seven different plasma lots. % CV for LLOQ samples was observed as 11.9 %, which are within 20.00% of the acceptance criteria. The within-run coefficients of variation ranged between 0.311 % and 0.601 % for cardiazol. The within-run percentages of nominal concentrations ranged between 99.80 % and 100.41 % for cardiazol. The between-run coefficients of variation ranged between 0.332 % and 0.615 % for cardiazol. The between-run percentages of nominal concentrations ranged between 98.18 % and 101.21 % for cardiazol. Conclusion: A rapid method was developed for simultaneous determination of cardiazol in human plasma. The method was strictly validated according to the ICH guidelines. Acquired results demonstrate that the proposed strategy can be effortlessly and advantageously applied for routine examination of cardiazol in human plasma.

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