Fermentation Quality and in Vitro Nutrient Digestibility of Fresh Rice Straw-Based Silage Treated with Lactic Acid Bacteria

The objective of this study was to investigate alginate microencapsulated lactic acid bacteria (LAB) fermentation quality of radish kimchi sample and its potential survivability in different acidic and alkaline environments. Initially, we isolated 45 LAB strains. One of them showed fast growth pattern with potential probiotic and antifungal activities against Aspergillus flavus with a zone of inhibition calculated with 10, 8, 4mm for the 4th, 5th, and 6th day, respectively. Therefore, this strain (KCC-42) was chosen for microencapsulation with alginate biopolymer. It showed potential survivability in in-vitro simulated gastrointestinal fluid and radish kimchi fermentation medium. The survival rate of this free and encapsulated LAB KCC-42 was 6.85 × 105 and 7.48× 105 CFU/ml, respectively; the viability count was significantly higher than nonencapsulated LAB in simulated gastrointestinal juices (acid, bile, and pancreatin) and under radish kimchi fermentation environment. Kimchi sample added with this encapsulated LAB showed increased production of organic acids compared to nonencapsulated LAB sample. Also, the organic acids such as lactic acid, acetic acid, propionic acid, and succinic acid production in fermented kimchi were measured 59mM, 26mM, 14mM, and 0.6mM of g/DW, respectively. The production of metabolites such as lactic acid, acetic acid, and succinic acid and the bacteria population was high in microencapsulated LAB samples compared with free bacteria added kimchi sample. Results of this study indicate that microencapsulated LAB KCC-42 might be a useful strategy to develop products for food and healthcare industries.

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Fermentation quality and in vitro methane production of sorghum silage prepared with cellulase and lactic acid bacteria

Asian-Australasian Journal of Animal Sciences ◽

10.5713/ajas.16.0502 ◽

2017 ◽

Vol 30 (11) ◽

pp. 1568-1574 ◽

Cited By ~ 9

Author(s):

Waroon Khota ◽

Suradej Pholsen ◽

David Higgs ◽

Yimin Cai

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Methane Production ◽

Fermentation Quality ◽

Sorghum Silage

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Manipulation of Rice Straw Silage Fermentation with Different Types of Lactic Acid Bacteria Inoculant Affects Rumen Microbial Fermentation Characteristics and Methane Production

Veterinary Sciences ◽

10.3390/vetsci8060100 ◽

2021 ◽

Vol 8 (6) ◽

pp. 100

Author(s):

Ehsan Oskoueian ◽

Mohammad Faseleh Jahromi ◽

Saeid Jafari ◽

Majid Shakeri ◽

Hieu Huu Le ◽

...

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Rice Straw ◽

Methane Production ◽

Dry Matter ◽

In Vitro Study ◽

Microbial Populations ◽

Different Types ◽

Silage Quality

Bacterial inoculants are known to improve the quality of silage. The objectives of the present study were to evaluate the effects of different types of lactic acid bacteria (LAB; L. plantarum, L. salivarius, L. reuteri, L. brevi, and S. bovis) inoculation (106 cfu/ DM) on rice straw silage quality and to determine these effects on ruminal fermentation characteristics, digestibility and microbial populations in an in vitro condition. Inoculated rice straw was ensiled for 15 and 30 days. For the in vitro study, rumen fluid was obtained from three rumen-fistulated bulls fed on mixed forage and concentrate at 60:40 ratio twice daily. Inoculation with LAB improved (p < 0.05) the rice straw silage quality as indicated by higher dry matter and crude protein contents, decreased pH and butyric acid, and increased propionic acid and LAB numbers, especially after 30 days of ensiling. Results from the in vitro study revealed that starting with the addition of LAB to rice straw silage improved in vitro fermentation characteristics such as increased total volatile fatty acids and dry matter digestibility (p < 0.05). LAB treatments also decreased methane production and methane/total gas ratio after 15 and 30 days of ensiling. From the rumen microbial population perspective, cellulolytic, and fungal zoospores were enhanced, while protozoa and methanogens were decreased by the LAB treatments. Based on these results, it could be concluded that inoculating rice straw silage with LAB (especially for L. plantarum and S. bovis) improved silage quality, rumen fermentation parameters and microbial populations in vitro.

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Using Lactic Acid Bacteria as Silage Inoculants or Direct-Fed Microbials to Improve In Vitro Degradability and Reduce Methane Emissions in Dairy Cows

Agronomy ◽

10.3390/agronomy10101482 ◽

2020 ◽

Vol 10 (10) ◽

pp. 1482

Author(s):

Nguyen Thi Huyen ◽

Ines Martinez ◽

Wilbert Pellikaan

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Dry Matter ◽

Gas Production ◽

Nutrient Digestibility ◽

Ch4 Production ◽

Silage Quality ◽

Silage Inoculants ◽

Silage Inoculant

The current study has two objectives: (1) To determine the effect of different lactic acid bacteria (LAB) strains’ inoculant on silage quality of fresh ryegrass (FR) and rain-treated ryegrass (RTR), and (2) to find the optimal way (silage inoculant vs. direct-fed microbial (DFM)) to use LAB strains in order to improve nutrient digestibility and reduce methane emission (CH4) in ruminant production. Five LAB strains were tested, Lactiplantibacillus plantarum AGR-1, L. plantarum AGR-2, Lactococcus lactis subsp. lactis biovar diacetylactis AGR-3, L. lactis subsp. lactis AGR-4 and L. lactis subsp. lactis AGR-5. Each LAB strain was inoculated at 106 cfu/g fresh weight into the FR and the RTR and ensiled for 60 days. After ensiling, the effect of LAB strains included as a DFM or silage inoculant on rumen digestibility and CH4 production were measured using an in vitro gas production system with three separate runs. The in vitro experiment consisted of 24 treatments (2 grasses (FR and RTR) × 2 ways (inoculant or DFM) × 6 strains (5 LAB strains + 1 Control)). The results indicated that the LAB strains’ inoculant treatments reduced (p < 0.0001) the dry matter (DM) losses, the NH3 concentration (p < 0.0001) and the pH (p = 0.0019) upon ensiling in both the FR and the RTR. The lowest values in dry matter (DM) loss and NH3 concentration were found in the L. plantarum (AGR-2) and L. lactis (AGR-5). The in vitro CH4 production was lower for silages inoculated with L. plantarum (AGR-1, p = 0.0054), L. lactis (AGR-4, p = 0.026), L. lactis (AGR-5, p = 0.029) and L. plantarum (AGR-2, p = 0.090), compared to the control. Methane production was lower (p = 0.0027) for LABs when used as silage inoculants, compared to being used as DFM. Lactic acid bacteria used as silage inoculants increased (p ≤ 0.0001) the in vitro DM and organic matter (OM) degradability both in the FR and the RTR, whereas LAB strains used as DFM showed no such effect. The DM and OM digestibility were highest in the L. plantarum (AGR-1, p = 0.0175). Among the five LAB strains used in the current study, L. plantarum (AGR-2) was the best candidate to improve silage quality. Our observations suggest that these LAB strains are most promising when used as silage inoculants and to be confirmed in vivo.

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